[ Objedlve] To investigate the effects of tea polyphenols （TP） and vitamin E （VE ） on growth performance and antioxidant performance of broilers in the routine breeding conditions. [ Method] A total of 270 broiler...[ Objedlve] To investigate the effects of tea polyphenols （TP） and vitamin E （VE ） on growth performance and antioxidant performance of broilers in the routine breeding conditions. [ Method] A total of 270 broilers at 1 day old were fed on the basic diets supplemented with TP and VE at different proportions. The malondialdehyde （MDA） content, total antioxidant capacity （T-AOC） as well as activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） were measured. The whole weight gain and feed conversion rate were also determined. [ Result] The broilers fed on TP and VE had remarkably higher serum activities of SOD and GSH-Px, stronger T-AOC and lower MDA content than the control ones. The best effects were achieved when the supplementary dosage of -rP and VE was respectively 60 and 80 mg/kg · Diet, because the experi- mental broilers had significantly higher antioxidant indices than the control ones （ P 〈 0.01 ）. Additionally, the boilers at the age of 21 or 42 days old had significantly higher body weight and lower feed conversion rate than the control ones （ P 〈 0.01 ）. [ Conclusion] The study provides a theoreti- cal basis for the applications of TP and VE in animal production.展开更多
Electronic nose has been applied in analysis of food products by monitoring their flavours.But it gives an overall response to a mixture of volatile components.In this work,multianalysis and chemometrics technique wer...Electronic nose has been applied in analysis of food products by monitoring their flavours.But it gives an overall response to a mixture of volatile components.In this work,multianalysis and chemometrics technique were employed to analyse the E-nose and GC-MS data of Longjing tea produced in different locations in Zhejiang province,China.A good discrimination of Longjing tea samples was obtained by E-nose according to the producing area,and a total of 38 compounds were commonly identified from the GC-MS data.Each individual volatile component was related to E-nose response using PLSR and the significance of each component was evaluated by the coefficient R2.Results indicated that isoamyl isovalerate,cis-3-hexenyl hexanoate,cadinene,phenylethylalcohol and linalool played an important role in response on E-nose sensors.It was possible to give a complementary information concerning the individual chemicals interacts with the sensors of E-nose instead of the total volatile components.展开更多
Eucommia ulmoides male flowers are rich in secondary metabolite,which have anti-tumor,sedative hypnotic,hypotensive,hypolipidemic,anti-fatigue,bacteriostatic,antioxidant and anti-aging effects.The conventional process...Eucommia ulmoides male flowers are rich in secondary metabolite,which have anti-tumor,sedative hypnotic,hypotensive,hypolipidemic,anti-fatigue,bacteriostatic,antioxidant and anti-aging effects.The conventional processing of E.ulmoides male flowers leads to the loss of nutrients and active ingredients.In recent years,cell wall breaking technology has been developed and utilized in various fields such as traditional Chinese medicine,good,chemical industry and biology.In order to promote the development of the E.ulmoides industry,the cell wall breaking technology and its characteristics are reviewed,and the application advantages of the cell wall breaking technology in the male flowers of E.ulmoides are discussed,and the prospect of the cell wall breaking of E.ulmoides is proposed in this article.展开更多
目的:明确维生素E琥珀酸酯(vitamin E succinate,VES)诱导的人口腔鳞癌Tca8113细胞的凋亡作用,初步探讨其凋亡机制。方法:PI单染色和Annexin Ⅴ-PI双染色后,流式细胞术检测VES对细胞周期的影响及细胞凋亡率;RT-PCR、west-ernblot法检测...目的:明确维生素E琥珀酸酯(vitamin E succinate,VES)诱导的人口腔鳞癌Tca8113细胞的凋亡作用,初步探讨其凋亡机制。方法:PI单染色和Annexin Ⅴ-PI双染色后,流式细胞术检测VES对细胞周期的影响及细胞凋亡率;RT-PCR、west-ernblot法检测促凋亡基因bax在mRNA水平和蛋白水平上的表达。结果:VES能显著抑制Tca8113细胞的生长,诱导出现典型的凋亡峰,bax基因及蛋白表达上调。结论:VES能诱导口腔鳞癌细胞Tca8113的凋亡,该作用可能与bax基因表达上调有关。展开更多
基金supported by Hebei Education Department(2010252)
文摘[ Objedlve] To investigate the effects of tea polyphenols （TP） and vitamin E （VE ） on growth performance and antioxidant performance of broilers in the routine breeding conditions. [ Method] A total of 270 broilers at 1 day old were fed on the basic diets supplemented with TP and VE at different proportions. The malondialdehyde （MDA） content, total antioxidant capacity （T-AOC） as well as activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） were measured. The whole weight gain and feed conversion rate were also determined. [ Result] The broilers fed on TP and VE had remarkably higher serum activities of SOD and GSH-Px, stronger T-AOC and lower MDA content than the control ones. The best effects were achieved when the supplementary dosage of -rP and VE was respectively 60 and 80 mg/kg · Diet, because the experi- mental broilers had significantly higher antioxidant indices than the control ones （ P 〈 0.01 ）. Additionally, the boilers at the age of 21 or 42 days old had significantly higher body weight and lower feed conversion rate than the control ones （ P 〈 0.01 ）. [ Conclusion] The study provides a theoreti- cal basis for the applications of TP and VE in animal production.
基金financial support from the China Agriculture (tea) Research System (CARS)Chinese Universities Scientific FundScientific Research Project of Zhejiang Education Department (Y201018805)
文摘Electronic nose has been applied in analysis of food products by monitoring their flavours.But it gives an overall response to a mixture of volatile components.In this work,multianalysis and chemometrics technique were employed to analyse the E-nose and GC-MS data of Longjing tea produced in different locations in Zhejiang province,China.A good discrimination of Longjing tea samples was obtained by E-nose according to the producing area,and a total of 38 compounds were commonly identified from the GC-MS data.Each individual volatile component was related to E-nose response using PLSR and the significance of each component was evaluated by the coefficient R2.Results indicated that isoamyl isovalerate,cis-3-hexenyl hexanoate,cadinene,phenylethylalcohol and linalool played an important role in response on E-nose sensors.It was possible to give a complementary information concerning the individual chemicals interacts with the sensors of E-nose instead of the total volatile components.
基金Supported by Project of Department of Education of Hunan Province(13CY017)
文摘Eucommia ulmoides male flowers are rich in secondary metabolite,which have anti-tumor,sedative hypnotic,hypotensive,hypolipidemic,anti-fatigue,bacteriostatic,antioxidant and anti-aging effects.The conventional processing of E.ulmoides male flowers leads to the loss of nutrients and active ingredients.In recent years,cell wall breaking technology has been developed and utilized in various fields such as traditional Chinese medicine,good,chemical industry and biology.In order to promote the development of the E.ulmoides industry,the cell wall breaking technology and its characteristics are reviewed,and the application advantages of the cell wall breaking technology in the male flowers of E.ulmoides are discussed,and the prospect of the cell wall breaking of E.ulmoides is proposed in this article.
文摘目的:明确维生素E琥珀酸酯(vitamin E succinate,VES)诱导的人口腔鳞癌Tca8113细胞的凋亡作用,初步探讨其凋亡机制。方法:PI单染色和Annexin Ⅴ-PI双染色后,流式细胞术检测VES对细胞周期的影响及细胞凋亡率;RT-PCR、west-ernblot法检测促凋亡基因bax在mRNA水平和蛋白水平上的表达。结果:VES能显著抑制Tca8113细胞的生长,诱导出现典型的凋亡峰,bax基因及蛋白表达上调。结论:VES能诱导口腔鳞癌细胞Tca8113的凋亡,该作用可能与bax基因表达上调有关。
