Seaweed has been used in traditional cosmetics and as a herbal medicine in treatments for cough,boils,goiters,stomach ailments,and urinary diseases,and for reducing the incidence of tumors,ulcers,and headaches.Despite...Seaweed has been used in traditional cosmetics and as a herbal medicine in treatments for cough,boils,goiters,stomach ailments,and urinary diseases,and for reducing the incidence of tumors,ulcers,and headaches.Despite the fact that seaweeds are frequently used in the practice of human health,little is known about the role of seaweed in the context of inflammation.This study aimed to investigate the influence of Jeju endemic seaweed on a mouse macrophage cell line(RAW 264.7) under the stimulation of lipopolysaccharide(LPS).Ethyl acetate extracts obtained from 14 different kinds of Jeju seaweeds were screened for inhibitory effects on pro-inflammatory mediators.Our results revealed that extracts from five seaweeds,Laurencia okamurae,Grateloupia elliptica,Sargassum thun-bergii,Gloiopeltis furcata,and Hizikia fusiformis,were potent inhibitors of the production of pro-inflammatory mediators such as nitric oxide(NO),prostaglandin E2(PGE2),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α).Based on these results,the anti-inflammatory effects and low cell toxicity of these seaweed extracts suggest potential thera-peutic applications in the regulation of the inflammatory response.展开更多
文摘目的 观察川芎嗪(TMP)对嘌呤2 X(P2 X)受体激动剂[三磷酸腺苷(ATP)和α,β-亚甲三磷酸腺苷(α,β- me ATP) ]、前列腺素E2 (PGE2 )及P物质(SP)所致大鼠足底急性伤害性反应的影响。方法 通过大鼠痛行为反应确定局部应用TMP对ATP等P2 X受体激动剂、PGE2 及SP所致大鼠足底急性伤害性反应和足底炎症水肿的影响。结果 TMP (10 mm ol/L )明显抑制ATP (1μm ol/L )或α,β- m e ATP (0 .6μm ol/L )引起的大鼠足底急性伤害性反应。TMP(10 m mol/L)可抑制PGE2 (5 μmol/L)或α,β- me ATP(0 .2 μm ol/L)加PGE2 (5 μmol/L )引起的伤害性反应。TMP (10 m mol/L )不影响α,β- me ATP (0 .2μmol/L )加SP (10μmol/L )引起的伤害性反应。TMP对PGE2 、SP或α,β- m e ATP分别加PGE2 或SP引起的大鼠足底炎症水肿无明显影响。结论 TMP主要通过抑制P2 X受体兴奋介导的伤害性信息传递产生抗伤害性反应作用。
基金supported by the Jeju Sea-green Program for the Regional Innovation Systemthe Regional Technology Innovation Program (No RTI04-02-07) of the Ministry of Knowledge and Economy,Korea
文摘Seaweed has been used in traditional cosmetics and as a herbal medicine in treatments for cough,boils,goiters,stomach ailments,and urinary diseases,and for reducing the incidence of tumors,ulcers,and headaches.Despite the fact that seaweeds are frequently used in the practice of human health,little is known about the role of seaweed in the context of inflammation.This study aimed to investigate the influence of Jeju endemic seaweed on a mouse macrophage cell line(RAW 264.7) under the stimulation of lipopolysaccharide(LPS).Ethyl acetate extracts obtained from 14 different kinds of Jeju seaweeds were screened for inhibitory effects on pro-inflammatory mediators.Our results revealed that extracts from five seaweeds,Laurencia okamurae,Grateloupia elliptica,Sargassum thun-bergii,Gloiopeltis furcata,and Hizikia fusiformis,were potent inhibitors of the production of pro-inflammatory mediators such as nitric oxide(NO),prostaglandin E2(PGE2),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α).Based on these results,the anti-inflammatory effects and low cell toxicity of these seaweed extracts suggest potential thera-peutic applications in the regulation of the inflammatory response.
