[ Objective] Based on salicylic acid culture solution, high performance liquid chromatography （HPLC） was used to rapidly count E. coli in water. [Method] Because E. coli metabolism could produce polyatomic phenol （...[ Objective] Based on salicylic acid culture solution, high performance liquid chromatography （HPLC） was used to rapidly count E. coli in water. [Method] Because E. coli metabolism could produce polyatomic phenol （mainly 2, 5-dihydroxybenzoic acid） in salicylic acid culture solu- tion, and generated amount of polyatomic phenol was proportionate to the number of E. coli, we detected 2, 5-dihydroxybenzoic acid by HPLC. Through linear relationship, the number of E. co/i was calculated, realizing fast counting of E. coli, and detection time only needed 3 h. [ Result] Linearity range of the method detecting E. coli was 5 × 10^4 -5 × 10^10cells/ml, and the detection limit was 1 × 10^4 cells/ml. [ Conclusion] HPLC method realized rapid counting of E. coil Key words E. coli; Fast counting; HPLC; Salicylic acid; China展开更多
基金Supported by Plan Item of National Bureau of Quality Inspection,China(2011QK345)Natural Science Foundation Item of Guangxi,China(Guikezi0728048)
文摘[ Objective] Based on salicylic acid culture solution, high performance liquid chromatography （HPLC） was used to rapidly count E. coli in water. [Method] Because E. coli metabolism could produce polyatomic phenol （mainly 2, 5-dihydroxybenzoic acid） in salicylic acid culture solu- tion, and generated amount of polyatomic phenol was proportionate to the number of E. coli, we detected 2, 5-dihydroxybenzoic acid by HPLC. Through linear relationship, the number of E. co/i was calculated, realizing fast counting of E. coli, and detection time only needed 3 h. [ Result] Linearity range of the method detecting E. coli was 5 × 10^4 -5 × 10^10cells/ml, and the detection limit was 1 × 10^4 cells/ml. [ Conclusion] HPLC method realized rapid counting of E. coil Key words E. coli; Fast counting; HPLC; Salicylic acid; China
