Turnip mosaic virus(TuMV)constitutes one of the primary diseases affecting Brassica rapa,severely impacting its production and resulting in crop failures in various regions worldwide.Recent research has demonstrated t...Turnip mosaic virus(TuMV)constitutes one of the primary diseases affecting Brassica rapa,severely impacting its production and resulting in crop failures in various regions worldwide.Recent research has demonstrated the significance of plant translation initiation factors,specifically the eIF4E and eIF4G family genes,as essential recessive disease resistance genes.In our study,we conducted evolutionary and gene expression studies,leading us to identify e IF(iso)4E.c as a potential TuMV-resistant gene.Leveraging CRISPR/Cas9 technology,we obtained mutant B.rapa plants with edited eIF(iso)4E.c gene.We confirmed eIF(iso)4E.c confers resistance against TuMV through phenotypic observations and virus content evaluations.Furthermore,we employed ribosome profiling assays on eif(iso)4e.c mutant seedlings to unravel the translation landscape in response to TuMV.Interestingly,we observed a moderate correlation between the fold changes in gene expression at the transcriptional and translational levels(R^(2)=0.729).Comparative analysis of ribosome profiling and RNA-seq data revealed that plant-pathogen interaction,and MAPK signaling pathway-plant pathways were involved in eIF(iso)4E.c-mediated TuMV resistance.Further analysis revealed that sequence features,coding sequence length,and normalized minimal free energy,influenced the translation efficiency of genes.Our study highlights that the loss of e IF(iso)4E.c can result in a highly intricate translation mechanism,acting synergistically with transcription to confer resistance against TuMV.展开更多
It has been made a proposal of new ideal cycle for power plants which is working by a turbine gas, for both closed and open systems. It has been designed a special device for adding heat at constant volume. The aim of...It has been made a proposal of new ideal cycle for power plants which is working by a turbine gas, for both closed and open systems. It has been designed a special device for adding heat at constant volume. The aim of special device is to decrease the amount of added heat for the new cycle. We have made a comparison between the simple gas turbine cycle & the new cycle. The results has been shown, that the efficiency of new cycle is greater than the simple cycle of gas turbine.展开更多
With the purpose of efficiently predicting structural radiated noise of internal combustion engine（I.C.E.）,a new simulation technique is introduced,which is an approach based on boundary element method （BEM）,acous...With the purpose of efficiently predicting structural radiated noise of internal combustion engine（I.C.E.）,a new simulation technique is introduced,which is an approach based on boundary element method （BEM）,acoustic transfer vector（ATV） technique and coupled boundary element model and finite element model （BEM-FEM） approach.Analyses of vibration exciting loads,computing structural dynamic characteristics and dynamic responses have led to theoretical results,which are tested on an L6 diesel engine to validate this proposed technique in engineering practice.展开更多
The adoption of applicable artificial intelligence technologies to library real-time virtual reference services is an innovative experimentation in one of the key areas of library services. Based on the open source so...The adoption of applicable artificial intelligence technologies to library real-time virtual reference services is an innovative experimentation in one of the key areas of library services. Based on the open source software Artificial Linguistic Internet Computer Entity(A.L.I.C.E.) and a combined application of several other relevant supporting technologies for facilitating the use of the current existing library resources, Tsinghua University Library has recently developed a real-time smart talking robot, named Xiaotu,for the enhancement of its various service functions, such as reference services, book searching, Baidu Baike searching, self-directed learning, etc. The operation of Xiaotu is programmed into Renren website(a social networking website), which adds significantly an innovative feature to the modus operandi of the real-time virtual reference service at Tsinghua University Library.展开更多
基金supported by grants from the Scientist Training Program of BAAFS (Grant No.JKZX202406)the Innovation and Capacity-Building Project of BAAFS (Grant No.KJCX20230221)+2 种基金Collaborative innovation program of the Beijing Vegetable Research Center (Grant No.XTCX202302)the National Natural Science Foundation of China (Grant No.32072567)the China Agriculture Research System of MOF and MARA (Grant No.CARS-A03)。
文摘Turnip mosaic virus(TuMV)constitutes one of the primary diseases affecting Brassica rapa,severely impacting its production and resulting in crop failures in various regions worldwide.Recent research has demonstrated the significance of plant translation initiation factors,specifically the eIF4E and eIF4G family genes,as essential recessive disease resistance genes.In our study,we conducted evolutionary and gene expression studies,leading us to identify e IF(iso)4E.c as a potential TuMV-resistant gene.Leveraging CRISPR/Cas9 technology,we obtained mutant B.rapa plants with edited eIF(iso)4E.c gene.We confirmed eIF(iso)4E.c confers resistance against TuMV through phenotypic observations and virus content evaluations.Furthermore,we employed ribosome profiling assays on eif(iso)4e.c mutant seedlings to unravel the translation landscape in response to TuMV.Interestingly,we observed a moderate correlation between the fold changes in gene expression at the transcriptional and translational levels(R^(2)=0.729).Comparative analysis of ribosome profiling and RNA-seq data revealed that plant-pathogen interaction,and MAPK signaling pathway-plant pathways were involved in eIF(iso)4E.c-mediated TuMV resistance.Further analysis revealed that sequence features,coding sequence length,and normalized minimal free energy,influenced the translation efficiency of genes.Our study highlights that the loss of e IF(iso)4E.c can result in a highly intricate translation mechanism,acting synergistically with transcription to confer resistance against TuMV.
文摘It has been made a proposal of new ideal cycle for power plants which is working by a turbine gas, for both closed and open systems. It has been designed a special device for adding heat at constant volume. The aim of special device is to decrease the amount of added heat for the new cycle. We have made a comparison between the simple gas turbine cycle & the new cycle. The results has been shown, that the efficiency of new cycle is greater than the simple cycle of gas turbine.
基金Sponsored by the National Natural Science Foundation of China (50505047)Program for the Top Young Academic Leaders of Higher Learning Institutions of Shanxi
文摘With the purpose of efficiently predicting structural radiated noise of internal combustion engine（I.C.E.）,a new simulation technique is introduced,which is an approach based on boundary element method （BEM）,acoustic transfer vector（ATV） technique and coupled boundary element model and finite element model （BEM-FEM） approach.Analyses of vibration exciting loads,computing structural dynamic characteristics and dynamic responses have led to theoretical results,which are tested on an L6 diesel engine to validate this proposed technique in engineering practice.
基金supported by the Foundation for Humanities and Social Sciences of the Chinese Ministry of Education(Grant No.:11BTQ011)
文摘The adoption of applicable artificial intelligence technologies to library real-time virtual reference services is an innovative experimentation in one of the key areas of library services. Based on the open source software Artificial Linguistic Internet Computer Entity(A.L.I.C.E.) and a combined application of several other relevant supporting technologies for facilitating the use of the current existing library resources, Tsinghua University Library has recently developed a real-time smart talking robot, named Xiaotu,for the enhancement of its various service functions, such as reference services, book searching, Baidu Baike searching, self-directed learning, etc. The operation of Xiaotu is programmed into Renren website(a social networking website), which adds significantly an innovative feature to the modus operandi of the real-time virtual reference service at Tsinghua University Library.
