Roles of Aqueous Extract of Marigold on Arsenic-Induced Oxidative Damage in Pancreatic Islet β-Cells

Roles of Marigold extracts (ME) on arsenic trioxide (ATO)-induced oxidative damage to pancreatic β-cells need to be further elucidated. In this study, NIT-1 cells were treated with different concentrations of and/or ATO, following by the cell viability was detected by CCK8 assay. Then, intracellular reactive oxygen species (ROS) levels, lipid peroxide (MDA) contents and superoxide dismutase (SOD) activity were measured with a fluorescence probe method and colorimetric assay, respectively. The apoptosis rate and morphology was detected and observed with hoechst 33,258 staining assay. The mRNA levels and protein expressions of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were measured by real-time fluorescence quantitative polymerase chain reaction and protein immunoblotting assay, respectively. Our results indicated that Co-treatment with ME and ATO exacerbated the cell viability decreasing reduced by ATO, while the addition of ME after ATO treatment effectively promote the recovery of ATO reduced survival rates. The ATO group increased apoptosis (P P β-cells by modulating the activation of the Nrf2 signaling pathway.

面向电商领域的旗袍知识图谱构建与实证研究

为挖掘旗袍服饰要素的关联关系与隐藏价值,构建一个基于电商数据的细粒度旗袍知识图谱,并开展实证研究。旗袍知识图谱采用自底向上逐层构建的方法,首先根据先验知识设计细粒度本体模型,为后续工作提供逻辑基础;然后采集并预处理原始数据,通过知识抽取、知识融合规范化数据,进行实例填充,完成数据层设计;最后将数据存储到图数据库,实现可视化。在此基础上,围绕旗袍、服饰要素以及用户人群开展深度挖掘、知识推理,借助图分析算法在社区检测、相似度和要素关联上展开实证研究。结果表明:旗袍知识图谱蕴含服饰要素之间隐藏的关系,能够挖掘内在的语义信息;旗袍服饰要素和用户人群之间存在一定的知识关联性,进而辅助设计与销售决策。

基于BIM的自动合规性审查研究综述

随着BIM技术在建筑领域的深度应用,越来越多的建筑设计开始以BIM模型方式进行交付,基于BIM的建筑工程设计自动合规性审查因其客观性、高效性受到理论和实践领域越来越多的关注。文章以基于BIM的自动合规性审查为主题进行综述,在Web of Science核心数据库对建筑领域自动合规性审查相关的研究文献进行全面检索及分析,从基本框架、规范条文信息抽取、BIM模型信息抽取及语义丰富、规范-模型信息匹配、合规性推理等方面对现有基于BIM的自动合规性审查研究进行系统解构,并对未来的研究方向进行讨论。最后,文章指出基于BIM的自动合规性审查在复杂文本信息抽取的性能与深度、模型语义丰富、信息匹配的自动化程度与通用性,以及计算机代码的透明度与灵活性等方面仍面临巨大挑战。

Box-Behnken响应面法结合多指标综合评分法优化俄色叶(变叶海棠)配方颗粒的提取工艺

目的:建立了俄色叶(变叶海棠)配方颗粒的最佳提取工艺条件。方法:采用高效液相色谱法同时测定俄色叶(变叶海棠)中的绿原酸、金丝桃苷、异槲皮苷、槲皮苷、根皮苷、槲皮素、根皮素的含量。在单因素试验的基础上,以浸泡时间、料液比、提取时间、提取次数为考察因素,上述7种成分含量、总黄酮、出膏率的综合评分为考察指标,采用Box-Behnken响应面法优化俄色叶(变叶海棠)配方颗粒的提取工艺。结果:俄色叶(变叶海棠)配方颗粒的最佳提取工艺为俄色叶饮片加水煎煮2次,第一次加15倍量水,浸泡30 min,煎煮45 min;第二次加13倍量水,煎煮45 min,滤过,合并煎液,即得。经过3次实验验证,所得平均综合评分为0.91(RSD=0%,n=3),与预测综合评分(0.938)的相对误差较小(3.08%)结论:Box-Behnken响应面法结合多指标综合评分法可用于俄色叶(变叶海棠)配方颗粒提取工艺优化,且所得最佳提取工艺稳定、简单、重现性好,可为俄色叶(变叶海棠)配方颗粒的制备及工业化大生产提供基础。

PRiME HLB固相萃取结合UPLC-QTOF-MS测定烟液与烟丝中的依托咪酯

待测样品经乙腈提取后,使用PRi ME HLB固相萃取柱对样品提取液进行净化,经ACE EXCEL C18(2.1mm×50mm,2μm)色谱柱分离,以甲醇-5mmol·L^(-1)甲酸铵水溶液(含0.1%甲酸)作为流动相,梯度洗脱,采用电喷雾离子源正模式(ESI+),目标物二级离子模式(Targeted MS/MS)进行质谱分析。依托咪酯在2ng·m L^(-1)~200ng·m L^(-1)浓度范围内线性关系良好,相关系数r为0.999,在0.2μg·g^(-1),1.0μg·g^(-1),5.0μg·g^(-1)3个水平的加标回收率为86.6%~94.6%,RSD为3.1%~4.3%(n=9)。本方法具有操作简易快速、定性定量准确等特点。

液液萃取/气相色谱-质谱法结合自动解卷积-Kováts保留指数法定性分析电子烟液中的挥发性成分

基于液液萃取/气相色谱-质谱法结合自动解卷积-Kováts保留指数法,建立了电子烟液中挥发性成分的检测方法。对提取溶剂种类进行了优化,并依据烟液中香气成分的香气特征,分析果味和烟草味烟液的香韵构成和差异,确定不同果味烟液的特征香气成分。结果表明:①以乙醚为提取溶剂能减弱丙三醇对其他组分识别的干扰。②自动解卷积法能准确识别共流出组分和低含量组分;Kováts保留指数法提升了化合物识别的准确度。③在10种烟草味烟液中检出15种成分,以豆香、焦香香韵化合物为主;在21种果味烟液中检出80种成分,以果香、花香香韵化合物为主,确定了16类果味烟液的特征香气成分。通过随机验证实验,该方法可有效分辨不同果味类型的果味烟液和烟草味烟液。该方法样品用量少、前处理过程简单、样品分析用时短,能满足烟液中香气成分的检测需要,可为果味电子烟液的检验识别和监管提供参考。

HPLC-MS/MS测定人脑脊液中多黏菌素E1和E2的方法及临床应用

目的建立一种新的盐析辅助液-液萃取结合高效液相色谱-串联质谱联用技术,快速测定人脑脊液中多黏菌素E1和E2浓度的方法。方法以多黏菌素B1为内标,用乙腈萃取脑脊液样本,甲酸铵作为盐析剂进行液-液萃取。并对盐析剂浓度、盐析剂体积以及萃取剂体积进行了优化。色谱柱采用Waters Xselect■HSS T3(2.1 mm×150 mm,2.5μm),流动相用乙腈和0.1%甲酸水溶液,采用梯度洗脱方式,检测时间6 min。结果在优选实验条件下,脑脊液中多黏菌素E1和E2分别在0.20~8.00μg·ml^(-1)(r=0.9960),0.35~14.12μg·ml^(-1)(r=0.9984)范围线性良好。待测物的准确度均在91.4%~109.0%范围,日内、日间精密度的相对标准偏差均低于9.5%。多黏菌素E1的提取回收率为77.7%~84.3%,多黏菌素E2的提取回收率为60.3%~68.3%。待测物在脑脊液中无明显基质效应。脑脊液样品在采集、储存、预处理过程中均能保持稳定。结论该方法操作简便,实验成本低,准确度高,重现性好,可应用于临床患者脑脊液中多黏菌素E1和E2的浓度测定。

融合特征提取的LMM模型在电商企业营销数量预测中的应用研究

准确的销售预测可以帮助电商企业制定合适的营销计划,以减少业务损失。但传统的预测模型对如今大数据发展迅速的时代来说,已经不能满足电商企业的需求。基于特征提取,对销售数据进行傅里叶变换处理,构建一种线性混合预测模型(Linear Mixed Model),应用至电商企业营销数量的预测中。开展实验对预测模型进行效用分析,实验表明,神经网络、支持向量机模型的MAE为0.04927154,0.04847517,而基于特征提取的线性混合模型的MAE更小,为0.04032,说明模型质量和性能更好。涉及RMSE、MAE、RMSE和RMSPE评估指标时,三种模型误差相差均在0.1以内。电商营销数量数据具有时间序列特性,但是线性混合模型在解决时间序列问题方面更有效,因此能够更加有效地处理电商数据。

基于CNN-GRU的文本数据特征提取及其分类技术设计

针对当下中文文本分类中存在的文本特征提取不足、分类准确率低等缺点,提出一种基于E-TF-IDF(Expand-Term Frequency-Inverse Document Frequency,E-TF-IDF)的关键词提取模型和CNN-GRU(Convolutional Neural Networks-Gated Recurrent Unit,CNN-GRU)的文本分类模型。该模型能够根据关键词邻近词语的出现概率中进行拓展,以实现更好的关键词特征提取。CNN-GRU更适用于序列分类且其具有更少的参数,能够减小在小数据集下的过拟合风险。最终的实验结果显示,CNN-GRU的分类精度较高,平均可达97.88%。

一种基于蓝牙射频取电实现电子纸标签电源技术方案

电子纸行业面临供能和续航挑战,BLE和射频技术的进步促成了蓝牙射频取电实现电子纸标签电源技术方案,有望解决行业痛点。提出蓝牙射频取电实现电子纸标签电源技术方案,介绍方案组成,详细阐述印刷电子纸技术、基于BLE通信的无源物联网系统、新零售管理系统,并对该方案进行展望,从而为电子纸行业的发展提供了新的动力和可能性。

Herbicidal Activities of Eupatorium adenophorum Extracts by 3 Extraction Methods

[ Objective ] The paper was to screen the better extraction method for herbicidal active substances of Eupatorium adenophorum. [ Method ] Three com- mon extraction methods, Soxhlet extraction, ultrasonic extraction and conventional extraction method, were used to prepare E. adenophorum extracts, and the herblcidal activity of E. adenophorum extracts extracted by 3 methods was studied by seed germination method. [ Result] The extraction rate of Soxhlet extraction was the highest of （22.54 ± 0.48 ）%, and the herbicidal activities of E. adenophorum extracted by Soxhlet extraction was relatively stronger. [ Conclusion] Soxhlet extraction was the better extraction method for herbicidal active substances of E. adenophorum.

Effect of diets supplemented with linseed alone or combined with vitamin E and selenium or with plant extracts,on Longissimus thoracis transcriptome in growing-finishing Italian Large White pigs

Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat products.Diet composition has been also demonstrated to influence the gene expression with effect on biological processes and pathways.However,the knowledge on the effect of nutrients at the molecular level is scant.In particular,studies on the effects of antioxidants and polyphenols dietary supplementation have been investigated mainly in rodents,and only scarcely in farm animals so far.RNA-Seq with next-generation sequencing is increasingly the method of choice for studying changes in the transcriptome and it has been recently employed also in pig nutrigenomics studies to identify diet-induced changes in gene expression.The present study aimed to investigate the effect of diets enriched with functional ingredients(linseed,vitamin E and plant extracts) on the transcriptome of pig Longissimus thoracis to elucidate the role of these compounds in influencing genes involved in muscle physiology and metabolism compared to a standard diet.Results: Eight hundred ninety-three significant differentially expressed genes(DEGs)(FDR adjusted P-value ≤ 0.05)were detected by RNA-Seq analysis in the three diet comparisons(D2-D1,D3-D1,D4-D1).The functional analysis of DEGs showed that the diet enriched with n-3 PUFA from linseed(D2) mostly downregulated genes in pathways and biological processes(BPs) related to muscle development,contraction,and glycogen metabolism compared to the standard diet.The diet supplemented with linseed and vitamin E/Selenium(D3) showed to mostly downregulate genes linked to oxidative phosphorylation.Only few genes involved in extracellular matrix(ECM)organization were upregulated by the D3.Finally,the comparison D4-D1 showed that the diet supplemented with linseed and plant extracts(D4) upregulated the majority of genes compared to D1 that were involved in a complex network of pathways and BPs all connected by hub genes.In particular,IGF2 was a hub gene connecting protein metabolism,ECM organization,immune system and lipid biosynthesis pathways.Conclusion: The supplementation of pig diet with n-3 PUFA from linseed,antioxidants and plant-derived polyphenols can influence the expression of a relevant number of genes in Longissimus thoracis muscle that are involved in a variety of biochemical pathways linked to muscle function and metabolism.

Optimization of Extraction Process for Polysaccharides of Urtica fissa E. Pritz.

[Objectives] To study the optimal extraction process for polysaccharides of Urtica fissa E. Pritz. [Methods] Leaves of wild U. fissa E. Pritz. collected in Nyingchi of Tibet were used as raw materials. Water extraction method was used to extract polysaccharides from U. fissa E. Pritz. Besides,comparison was made for the effects of extraction time,extraction temperature and solid-to-liquid ratio on the yield rate of polysaccharides from U. fissa E. Pritz. In addition,using the extraction time,extraction temperature and solid-to-liquid ratio as factors,L9( 33) orthogonal experiment was carried out to determine the optimal process parameters for water extraction of polysaccharides from U. fissa E. Pritz.[Results] The optimal process parameters: hot water extraction time was 4 h,the extraction temperature was 90℃,and the mass ratio of water to leaves of U. fissa E. Pritz. was 1∶ 25. Under such extraction conditions,the yield rate of polysaccharides from U. fissa E. Pritz.was up to 11. 611%. [Conclusions]This experiment is expected to provide certain reference for reasonable development and utilization of wild U. fissa E. Pritz. resources in Tibet.

Antibiotic resistant Esherichia coli strains from seafood and its susceptibility to seaweed extracts

Objective:To determine the prevalence and antibiotic resistance of Escherichia coli(E. coli),in seafood obtained from Cuddalore and Parangipettai fish landing centres.Also,to identify the susceptibility of E.coli against predominant seaweeds red alga Kappaphycus alvarezii(K.alvarezii) and brown alga Padina boergessenii(P.boergessenii) extracts as sulfated polysaccharides and polyphenols respectively.Methods:A total of 48 samples(Two stations Cuddalore and Parangipettai,Tamil Nadu,India).Sampling area are fish landing centre where fishes caught from sea and estuary,seafood processing plants(packing and ice packed fishes) and local fish markets(fish samples).After isolation totally 80 strains were analyzed for its antimicrobial resistance and sensitivity against commercially 10 antibiotics.The ampicillin resistant E.coli CE21 was identified through molecular techniques as I6S rDNA sequencing. Two seaweeds K.alvarezii and P.boergessenii were screened lor antibacterial activity against 12 antibiotic resistant E.coli strains.Results:Totally 48 swabbed samples from two different fish handling area were characterized for total bacterial and E.coli count.Mostly,the E.coli strains were isolated from fish local market and seafood processing plants before and after packaging process.In that maximum 56.25%strains were resistant to ampicillin and the minimum 2.5%strains were resistant to chloramphenicol.Therefore,the E.coli CE21 was identified through molecular techniques E.coli(GenBank accession number GU065251),The MIC value for polyphenol extract was slightly less than sulfated polysaccharides.E.coli strain isolated from Parangipettai was considerably increased MIC value that Cuddalore.Conclusions: The polyphenol and sulfated polysaccharides showed promising inhibitory response against all antimicrobial resistant E.coli strains and in particular the inhibitory response of ampicillin resistant E.coli.

The Protective Effect of Grape Seed Extract on Cardiotoxicity Induced by Doxorubicin Drug in Male Rats

Objective: This work was designed to determine the productive effect of grape seed proanthocynadine extract (GSPE) and Vitamin E against Doxorubicin (DOX) induced myocardial toxicity in 50 male. Wister rates were divided in five groups. The 1st group was untreated and served as a control. The 2nd group was treated with DOX only, the 3rd group was pretreated with GSPE, the 4th group was pretreated with Vitamin E, and the 5th group was pretreated with GSPE and Vitamin E. DOX was administered by single i.p (Intraperitonial) injection of 15 mg/kg/body weight to induce cardio toxicity and Vitamin E was administered at a dose of 400 IU/kg/bodyweight/day, p.o (per oral) for 10 days prior to DOX administration [1]. GSPE was given at a dose of 150 mg/kg/bodyweight/ day, p.o (per oral) for 10 days before treatment with DOX. After 2 weeks experimental period, blood samples and heart tissues were taken from all groups. The general observations, mortality, histopathology, biomarker enzymes like Lactate Dehydrogenase (LDH), Creatine Phosphokinase (CPK), Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Antioxidants such as Glutathione (GSH), Superoxide dismutase (SOD), Catalase (CAT) and Malondialdehyde (MDA) were monitored after 2 weeks of the last dose. Results: Administration of DOX caused cardiomyopathy associated with an antioxidant deficiency. Pretreatment with GSPE and Vitamin E significantly (P < 0.01) protected the myocardium from the toxic effects of DOX by reducing the elevated level of biomarkers and diagnostic enzymes like LDH, CPK, AST, and ALT to normal levels. GSPE and Vitamin E increased the GSH, SOD and CAT levels and decreased the MDA levels in cardiac tissue. Conclusion: These results suggest a cardioprotective effect of GSPE and Vitamin E due to its antioxidant properties.

Therapeutic effects of Byrsocarpus coccineus root bark extract on bacterially and chemically induced diarrhea in the Wistar albino rat(Rattus norvegicus domestica)

Background : Diarrhea can be caused by pathogenic microorganisms and chemicals. In view of this, Byrsocarpus coccineus Schum and Thonn(Connaraceae) was used to treat diarrhea induced by castor oil or bacteria in Wistar albino rats. Methods : Qualitative and quantitative analyses of an aqueous root back extract of B. coccineus were made and the acute toxicity, antidiarrhea properties, and in vitro and in vivo antimicrobial activities of the extract were investigated in rats. Results : The phytochemical analysis of the root bark extract revealed the presence of flavonoids, alkaloid, saponins, tannins, and phenols. The quantitative analysis showed that saponins formed 10.6% of the extract, tannins 7.6%, flavonoids 6.2%, phenol 5.8% and alkaloids 4.4%. A dose limit of 5000 mg/kg was safe to use in the rats. At a dose of 100 mg/kg, the extract decreased distance travelled by activated charcoal in the gastrointestinal tract, frequency of defecation, and number of unformed faeces caused by castor oil-induced diarrhea, and led to 74.96% inhibition of the diarrhea effects. Escherichia coli and Salmonella pullorum were susceptible to higher concentrations of the extract with a minimum inhibitory concentration of 0.3125 mg/m L. E. coli-infected rats showed depression, weight loss, anorexia, diarrhea, and weakness, which was ameliorated by the extract on day 2 post treatment. Observed congestion, cellular infiltration and necrosis of the liver, intestine and kidney following infection were improved by the extract. Conclusion : B. coccineus extract can be used in the treatment of anaemia, and castor oil-and E. coli-induced diarrhea in rats.

Optimization of Ultrasonic-enzymatic Extraction of Total Flavonoids from Cardamine tangutorum O.E.Schulz by Response Surface Methodology and Content Determination

[Objectives]This study aimed to optimize the extraction process of total flavonoids in Cardamine tangutorum O.E.Schulz.[Methods]On the basis of single-factor experiments,the ultrasonic-enzymatic extraction process of total flavonoids in C.tangutorum O.E.Schulz was optimized by Box-Behnken design from aspects of ethanol volume fraction,extraction time,extraction temperature and enzyme addition,with yield of total flavonoids as the evaluation index.[Results]The optimal extraction process of total flavonoids in C.tangutorum O.E.Schulz was as follows:ethanol volume fraction of 70%,ultrasonic extraction time of 43 min,ultrasonic extraction temperature of 37℃and cellulose addition of 0.07 mg.Under the optimal conditions,the yield of total flavonoids from C.tangutorum O.E.Schulz reached 75.90 mg/g.[Conclusions]The optimized extraction process was reasonable and feasible,and it could provide a reference for the extraction of total flavonoids in C.tangutorum O.E.Schulz.This study provided a foundation for the quality control of food/medicinal materials and the development and research of big health products of C.tangutorum O.E.Schulz.

Effect of the Ginseng extractive E on experimental climacteric disturbance in rats

Objective To search the effects of the extractive E of Ginseng(EG)on experimental climacteric syndrome in rats.Methods Extirpating the both sides of ovary of rats to turbulence estrogen secretion,induce climacteric syndrome.The weight,bite and sup,substance of bone,blood lipid,calcium urine biochemistry,estrodiol,behavior perfomance were also observed.Results The body weight of ovariotomied rat was controlled,bone density was increased,estradiol level increased,the weight of bone increased.Conclusions EG ameliorated climacteric syndrome.Increase bone density and bone mine content,enhanced the level of estradiol.

Effect of Extraction Methods on the Active Compounds and Antioxidant Properties of Ethanolic Extracts of Echinacea purpurea Flower

The extraction yields, active compounds and antioxidant properties of 50%-aqueous-ethanolic extracts of freeze-dried Echinacea purpurea flower with multi-steps and multi-batches extraction methods were assessed. In multi-steps extraction, the extraction yields of 1st, 2nd, and 3rd extracts were 21.52%, 9.33%, and 2.90%, and their total phenols contents were 182.08, 176.33, and 177.08 mg CAE/g, respectively, with cichoric acid (62.07 - 66.57 mg/g) being the main phenolic compound. No differences in the contents of individual and total caffeic acids derivates existed among 1st, 2nd, and 3rd extracts. The dodeca-2E, 4E, 8Z, 10(E/Z)-tetraenoic acid isobutylamide (alkamide 8/9) contents of 1st, 2nd, and 3rd extracts were 505.38, 598.61, and 585.99 &#181g/g, respectively. In multi-batches extraction, the extracted dry weight increased with increasing the sample batches, with the extraction yields and alkamide 8/9 contents of samples decreased from 19.93% to 12.98% and 534.36 to 269.76 &#181g/g, respectively. The total phenol (177.25 - 186.92 mg CAE/g), individual and total caffeic acid derivatives (85.99 - 95.06 mg/g) contents of extracts among different sample batches were not significantly different, with cichoric acid (63.66 - 70.31 mg/g) being the main phenolic compound. All the prepared extracts also exhibited potent antioxidant properties. Overall, the two-step sequential extraction is desirable for extracting bioactive compounds from freeze-dried E. purpurea flower.

A Simple Procedure for Extraction of Surface Protein of Salmonella Serotypes and Escherichia coli Strains Isolated from Poultry and Pigs

Salmonella and E.coli possess different surface protein structures that can induce protective immune responses.Identification of these proteins capacitates development of diverse applications in prevention and diagnosis that contribute to effectively control disease-causing enterobacteria pathogens such as Salmonella and E.coli.A simple procedure for obtaining protein complexes of Salmonella serotypes and E.coli is performed in this study.A sonication process with heat treatment of whole bacteria induced the release of protein complexes.Concentration of the protein extract was quantified using protein quantification Kits-Rapid,and protein complex profile was obtained by SDS-PAGE(Sodium dodecyl sulfate polyacrylamide gel electrophoresis)and silver staining.The concentrations of protein ranged from 29.45 to 45.35μg/mL in the Salmonella protein extracts,and from 25.35 to 36.72μg/mL in the E.coli protein extracts.Six major groups of proteins from E.coli(YfiO,NipB,OmpF,YfgL,Talc,YaeT)and four major groups of proteins from Salmonella(Flagellin,OmpA,Porin,SEF21)were preliminarily determined by a simple procedure of extraction based on the molecular weight.