Ammonium glycyrrhizinate is the amide of glycyrrhetinic acid which derives from the glycyrrhizin, the effective component of glycyrrhiza. Recent studies have proved that ammonium glycyrrhizinate plays an important rol...Ammonium glycyrrhizinate is the amide of glycyrrhetinic acid which derives from the glycyrrhizin, the effective component of glycyrrhiza. Recent studies have proved that ammonium glycyrrhizinate plays an important role in some vital pharmacological processes, like immunoregulation. In order to study the mechanisms of action of ammonium glycyrrhizinate at the enzyme molecular level, in this note,展开更多
目的：观察穴位埋线对原发性痛经模型小鼠的影响。方法：取体质量18~22 g雌性成年未孕小鼠40只,随机分成4组,空白对照组、模型组、阿司匹林组、穴位埋线组。实验开始时,空白对照组腹腔皮下注射生理盐水,每日1次（每只0.4 m L）,连续7 d...目的：观察穴位埋线对原发性痛经模型小鼠的影响。方法：取体质量18~22 g雌性成年未孕小鼠40只,随机分成4组,空白对照组、模型组、阿司匹林组、穴位埋线组。实验开始时,空白对照组腹腔皮下注射生理盐水,每日1次（每只0.4 m L）,连续7 d;其余组均连续腹腔皮下注射苯甲酸雌二醇7 d,每日1次（每只0.2 m L）,其中,穴位埋线组在造模第1天予以埋线治疗1次,取穴中极、十七椎下,阿司匹林组于造模第1天开始灌胃给药,连续7 d,每日1次（每只0.2 m L,浓度为0.2 g·m L^-1）,空白对照组和模型组不予以治疗,正常饲养,末次给药1 h后,空白对照组腹腔注射生理盐水每只0.4 m L,其余组腹腔注射缩宫素每只0.2 m L,观察0~20 min内各组小鼠疼痛反应,记录各组扭体次数及扭体发生率。眼眶后静脉丛采血1 m L,迅速注入含有3.8%柠檬酸三钠的塑料指形管中混匀,立即低温离心（4℃3 000 r·min^-1）15 min,取上清,置-70℃低温冰箱,以备PGE2、PG F2α检测。结果：与模型组相比,穴位埋线组小鼠20min内扭体次数显著减少（P〈0.01）,小鼠血浆PGE2含量明显升高（P〈0.01）,小鼠血浆PGF2α含量明显降低（P〈0.01）,差异有统计学意义;与阿司匹林组相比,差异无统计学意义（P〉0.05）。结论：穴位埋线治疗原发性痛经具有明显的镇痛作用;穴位埋线疗法有调节血浆前列腺素分泌的作用。展开更多
文摘Ammonium glycyrrhizinate is the amide of glycyrrhetinic acid which derives from the glycyrrhizin, the effective component of glycyrrhiza. Recent studies have proved that ammonium glycyrrhizinate plays an important role in some vital pharmacological processes, like immunoregulation. In order to study the mechanisms of action of ammonium glycyrrhizinate at the enzyme molecular level, in this note,
文摘目的：观察穴位埋线对原发性痛经模型小鼠的影响。方法：取体质量18~22 g雌性成年未孕小鼠40只,随机分成4组,空白对照组、模型组、阿司匹林组、穴位埋线组。实验开始时,空白对照组腹腔皮下注射生理盐水,每日1次（每只0.4 m L）,连续7 d;其余组均连续腹腔皮下注射苯甲酸雌二醇7 d,每日1次（每只0.2 m L）,其中,穴位埋线组在造模第1天予以埋线治疗1次,取穴中极、十七椎下,阿司匹林组于造模第1天开始灌胃给药,连续7 d,每日1次（每只0.2 m L,浓度为0.2 g·m L^-1）,空白对照组和模型组不予以治疗,正常饲养,末次给药1 h后,空白对照组腹腔注射生理盐水每只0.4 m L,其余组腹腔注射缩宫素每只0.2 m L,观察0~20 min内各组小鼠疼痛反应,记录各组扭体次数及扭体发生率。眼眶后静脉丛采血1 m L,迅速注入含有3.8%柠檬酸三钠的塑料指形管中混匀,立即低温离心（4℃3 000 r·min^-1）15 min,取上清,置-70℃低温冰箱,以备PGE2、PG F2α检测。结果：与模型组相比,穴位埋线组小鼠20min内扭体次数显著减少（P〈0.01）,小鼠血浆PGE2含量明显升高（P〈0.01）,小鼠血浆PGF2α含量明显降低（P〈0.01）,差异有统计学意义;与阿司匹林组相比,差异无统计学意义（P〉0.05）。结论：穴位埋线治疗原发性痛经具有明显的镇痛作用;穴位埋线疗法有调节血浆前列腺素分泌的作用。
