The objective of this paper was to analyze the E2 genetic characterization of HB-DCZ strain of Bovine viral diaxrhca Virus （BVDV） which wcrc amplified by RT-PCR and isolated from China. The product of PCK was cloned...The objective of this paper was to analyze the E2 genetic characterization of HB-DCZ strain of Bovine viral diaxrhca Virus （BVDV） which wcrc amplified by RT-PCR and isolated from China. The product of PCK was cloned into pMD18-T vector, and then transfected Escherichia Coli JMI00. The recombinant plasmids were amplified by PCR and were sequenced. From the nucleotide sequence of the amplified products, phylogenetie analyses were performed and genotypes or subgenotypes were identified. The results indicated that the E2 gene fragment of HB-DCZ strain contained 1277bp nucleotides, and had 89.4%, 70.7%, 97.6%, 68.9%, 67.2% sequence similarity with Osloss, OregonC24V, Changchun184, ZM195, NADL, respectively. In conclusion, HB-DCZ strain is closely related to BVDV Osloss, Changchun184, and belongs to subgenotype lb.展开更多
Zm ERECTA-LIKE2 sequences were isolated according to the Zea mays genome. The study indicates that zm ERECTALIKE2 is composed of 2 410 nucleotides and encodes a receptor-like kinase which consists of 774 amino acids b...Zm ERECTA-LIKE2 sequences were isolated according to the Zea mays genome. The study indicates that zm ERECTALIKE2 is composed of 2 410 nucleotides and encodes a receptor-like kinase which consists of 774 amino acids by biological analysis. The results show that zm ERECTA-LIKE2 protein contains 13 domains of leucine-rich repeats and belongs to the PKclike family, it is a liposoluble protein, with a conserved transmembrane region.展开更多
文摘The objective of this paper was to analyze the E2 genetic characterization of HB-DCZ strain of Bovine viral diaxrhca Virus （BVDV） which wcrc amplified by RT-PCR and isolated from China. The product of PCK was cloned into pMD18-T vector, and then transfected Escherichia Coli JMI00. The recombinant plasmids were amplified by PCR and were sequenced. From the nucleotide sequence of the amplified products, phylogenetie analyses were performed and genotypes or subgenotypes were identified. The results indicated that the E2 gene fragment of HB-DCZ strain contained 1277bp nucleotides, and had 89.4%, 70.7%, 97.6%, 68.9%, 67.2% sequence similarity with Osloss, OregonC24V, Changchun184, ZM195, NADL, respectively. In conclusion, HB-DCZ strain is closely related to BVDV Osloss, Changchun184, and belongs to subgenotype lb.
基金Supported by the Importation and Development of High-caliber Talents Project of Beijing Municipal Institutions(CIT and TCD 201304096)
文摘Zm ERECTA-LIKE2 sequences were isolated according to the Zea mays genome. The study indicates that zm ERECTALIKE2 is composed of 2 410 nucleotides and encodes a receptor-like kinase which consists of 774 amino acids by biological analysis. The results show that zm ERECTA-LIKE2 protein contains 13 domains of leucine-rich repeats and belongs to the PKclike family, it is a liposoluble protein, with a conserved transmembrane region.
