[Objective]The paper was to establish a fluorescence quantitative RT-PCR method for detection of equine arteritis virus(EAV).[Method]Primers and probes were developed for the EAV ORF7 gene sequence,and the reaction sy...[Objective]The paper was to establish a fluorescence quantitative RT-PCR method for detection of equine arteritis virus(EAV).[Method]Primers and probes were developed for the EAV ORF7 gene sequence,and the reaction system was optimized.Standard curves were established,leading to the initial development of the EAV fluorescence quantitative RT-PCR assay.The accuracy,specificity,and sensitivity of this method were subsequently evaluated.[Result]The EAV fluorescence quantitative RT-PCR assay demonstrated optimal performance at an annealing temperature of 61 C,with a final concentration of primer and probe set at 0.6μmol/L.The plasmid standard demonstrated a strong linear correlation with Ct values within the range of 1.6×10^(7)-1.6×10^(2)copies/μL.The equation of the standard curve was determined to be y=-2.68x+32.88,with an R^(2) value of 0.9927.Consequently,the EAV fluorescence quantitative RT-PCR assay was successfully established.The methodology employed was effective in detecting EAV,Theileria equi,equine herpesvirus-1(EHV-1),equine herpesvirus-4(EHV-4),and equine influenza virus(EIV).The findings indicated that the method was specifically capable of detecting EAV,while the other pathogens tested yielded negative results.The method demonstrated a high degree of specificity.It was employed to detect the standard plasmid cRNA synthesized through in vitro transcription following a 10-fold dilution.The results indicated that the minimum detection limit of the method was 1.6×10^(2) copies/μL,and it exhibited high sensitivity.The coefficient of variation,both within and between groups,was maintained at 1.8%,indicating good reproducibility.In this study,the fluorescence quantitative RT-PCR assay developed was utilized alongside the EAV fluorescence quantitative RT-PCR assay established by previous researchers to analyze a total of 234 clinical samples.Both methods yielded a positive detection rate of 14.1%,and the coincidence rate between the two techniques was found to be 100%.[Conclusion]The fluorescence quantitative RT-PCR assay developed in this study offers a novel approach and concept for the prevention and control of equine viral arteritis(EVA).展开更多
请先观察以下两对句子:1)a.Fish abound in this stream.b.This stream abounds in fish.这条小溪有大量的鱼。2)a.A curtain hung over the window.b.The window hung with a curtain.窗户上挂着窗帘。上述每对句子中,上下两句句意相同...请先观察以下两对句子:1)a.Fish abound in this stream.b.This stream abounds in fish.这条小溪有大量的鱼。2)a.A curtain hung over the window.b.The window hung with a curtain.窗户上挂着窗帘。上述每对句子中,上下两句句意相同,相映成趣。它们都可归纳为同一句型结构,即“主语+动词+介词+介词宾语”。在此结构中,主语和介词宾语的位置可以互换,但动词不变,且句意基本相同。Otto展开更多
Since ancient times there has been thislegendary saying in China:“It is onlythrough Shen Nong’s efforts in tasting mis-cellaneous herbs that Chinese medicine andpharmacology came to be.”This vividly il-lustrates th...Since ancient times there has been thislegendary saying in China:“It is onlythrough Shen Nong’s efforts in tasting mis-cellaneous herbs that Chinese medicine andpharmacology came to be.”This vividly il-lustrates the historical process of discoveringdrugs and gradually accumulating experi-ence by our ancestors in the course of展开更多
The present paper is aimed to better illustrate the possible association between a particular extremely weak field, the air ions, and the human health response to this solicitation. Methods and instruments for this ki...The present paper is aimed to better illustrate the possible association between a particular extremely weak field, the air ions, and the human health response to this solicitation. Methods and instruments for this kind of analysis are here given in the form of a project： a ＂Ions meter＂, to reveal and characterize the properties of the air ions, and ＂APEC 300＂, an advanced electronic device, both instruments are with a HPR （high power of resolution）. APEC 300 can give the response to the possible health effects by measurements of two electrocuteaneous parameter： the potential level and the impedance. The power of resolution of APEC 300 is much higher than that of other usual devices, for instance, the EAV ones （electroacupuncture）, this instrument will be completely ready within few weeks.展开更多
Located in the east of Gansu Province, Gangu County in Tianshui City is the only way that people went to the Central Plains from the western regions in ancient times, and it's also an important node of the Grotto ...Located in the east of Gansu Province, Gangu County in Tianshui City is the only way that people went to the Central Plains from the western regions in ancient times, and it's also an important node of the Grotto Culture Corridor of the Silk Road. Daxiang Mountain Giant Buddha Grottoes is the landmark of Gangu County. The giant Buddha statue stands among cliffs 200 meters high above the ground, and it is the second largest stone-base clay-sculpture Buddha in China. After going through thousands of years, the Giant Buddha Grottoes needs effective and scientific protection. This article was originated from the actual project—The Research on the Construction Technology of Wooden-structure Grotto Eaves in the Daxiang Mountain. This article f inished the rebuilding plan for the Buddha Grotto Eaves on the basis of analyzing historical documents, hole traces, morphological evolution and image demonstration.展开更多
My neighbor Mrs. Gargan first told me about it. 'Have you seenthe tree?' She asked as I was sitting in the back yard enjoying theOctober twilight. 'The one down at the corner,' she explained. 'It’...My neighbor Mrs. Gargan first told me about it. 'Have you seenthe tree?' She asked as I was sitting in the back yard enjoying theOctober twilight. 'The one down at the corner,' she explained. 'It’a beautifultree——all kinds of colors. Cars are stopping to, look. You ought tosee it.'展开更多
基金Supported by Research Project of General Administration of Customs(2022HK126)Youth Science Foundation(2022D01B08).
文摘[Objective]The paper was to establish a fluorescence quantitative RT-PCR method for detection of equine arteritis virus(EAV).[Method]Primers and probes were developed for the EAV ORF7 gene sequence,and the reaction system was optimized.Standard curves were established,leading to the initial development of the EAV fluorescence quantitative RT-PCR assay.The accuracy,specificity,and sensitivity of this method were subsequently evaluated.[Result]The EAV fluorescence quantitative RT-PCR assay demonstrated optimal performance at an annealing temperature of 61 C,with a final concentration of primer and probe set at 0.6μmol/L.The plasmid standard demonstrated a strong linear correlation with Ct values within the range of 1.6×10^(7)-1.6×10^(2)copies/μL.The equation of the standard curve was determined to be y=-2.68x+32.88,with an R^(2) value of 0.9927.Consequently,the EAV fluorescence quantitative RT-PCR assay was successfully established.The methodology employed was effective in detecting EAV,Theileria equi,equine herpesvirus-1(EHV-1),equine herpesvirus-4(EHV-4),and equine influenza virus(EIV).The findings indicated that the method was specifically capable of detecting EAV,while the other pathogens tested yielded negative results.The method demonstrated a high degree of specificity.It was employed to detect the standard plasmid cRNA synthesized through in vitro transcription following a 10-fold dilution.The results indicated that the minimum detection limit of the method was 1.6×10^(2) copies/μL,and it exhibited high sensitivity.The coefficient of variation,both within and between groups,was maintained at 1.8%,indicating good reproducibility.In this study,the fluorescence quantitative RT-PCR assay developed was utilized alongside the EAV fluorescence quantitative RT-PCR assay established by previous researchers to analyze a total of 234 clinical samples.Both methods yielded a positive detection rate of 14.1%,and the coincidence rate between the two techniques was found to be 100%.[Conclusion]The fluorescence quantitative RT-PCR assay developed in this study offers a novel approach and concept for the prevention and control of equine viral arteritis(EVA).
文摘请先观察以下两对句子:1)a.Fish abound in this stream.b.This stream abounds in fish.这条小溪有大量的鱼。2)a.A curtain hung over the window.b.The window hung with a curtain.窗户上挂着窗帘。上述每对句子中,上下两句句意相同,相映成趣。它们都可归纳为同一句型结构,即“主语+动词+介词+介词宾语”。在此结构中,主语和介词宾语的位置可以互换,但动词不变,且句意基本相同。Otto
文摘Since ancient times there has been thislegendary saying in China:“It is onlythrough Shen Nong’s efforts in tasting mis-cellaneous herbs that Chinese medicine andpharmacology came to be.”This vividly il-lustrates the historical process of discoveringdrugs and gradually accumulating experi-ence by our ancestors in the course of
文摘The present paper is aimed to better illustrate the possible association between a particular extremely weak field, the air ions, and the human health response to this solicitation. Methods and instruments for this kind of analysis are here given in the form of a project： a ＂Ions meter＂, to reveal and characterize the properties of the air ions, and ＂APEC 300＂, an advanced electronic device, both instruments are with a HPR （high power of resolution）. APEC 300 can give the response to the possible health effects by measurements of two electrocuteaneous parameter： the potential level and the impedance. The power of resolution of APEC 300 is much higher than that of other usual devices, for instance, the EAV ones （electroacupuncture）, this instrument will be completely ready within few weeks.
基金Sponsored by Program of Housing and Urban-rural Construction Department of Gansu Province(JK2014-22)
文摘Located in the east of Gansu Province, Gangu County in Tianshui City is the only way that people went to the Central Plains from the western regions in ancient times, and it's also an important node of the Grotto Culture Corridor of the Silk Road. Daxiang Mountain Giant Buddha Grottoes is the landmark of Gangu County. The giant Buddha statue stands among cliffs 200 meters high above the ground, and it is the second largest stone-base clay-sculpture Buddha in China. After going through thousands of years, the Giant Buddha Grottoes needs effective and scientific protection. This article was originated from the actual project—The Research on the Construction Technology of Wooden-structure Grotto Eaves in the Daxiang Mountain. This article f inished the rebuilding plan for the Buddha Grotto Eaves on the basis of analyzing historical documents, hole traces, morphological evolution and image demonstration.
文摘My neighbor Mrs. Gargan first told me about it. 'Have you seenthe tree?' She asked as I was sitting in the back yard enjoying theOctober twilight. 'The one down at the corner,' she explained. 'It’a beautifultree——all kinds of colors. Cars are stopping to, look. You ought tosee it.'
