Background:Herpesvirus-like particles have been reported to be detectable by electron microscopy in lesional biopsy of patients with pityriasis rosea(PR).We report a study investigating the association of PR with huma...Background:Herpesvirus-like particles have been reported to be detectable by electron microscopy in lesional biopsy of patients with pityriasis rosea(PR).We report a study investigating the association of PR with human herpesvirus-8(HHV-8)infection.Methods:Our setting is a teaching clinic affiliated to a university.We recruited eight patients aged 28-47 years(mean:34.5 years)diagnosed with PR during a one-year period.We collected acute blood specimens at presentation and convalescent blood specimens three to four weeks later.We also collected skin scrapings from the herald patch where present and from truncal secondary lesions.Results:We detected HHV-8 DNA by a nested PCR(polymerase chain reaction)targeting,respectively,a 233-bp and a 160-bp fragment of ORF 26.PCR for HHV-8 DNA was negative in the peripheral blood mononuclear cells and plasma of acute and convalescent specimens of all patients,and negative in all skin scrapings.We detected anti-HHV-8 IgG and IgM antibodies by the indirect immunofluorescence.Four patients had IgG antibodies against HHV-8,but with no significant rise of titre.None were positive for anti-HHV-8 IgM antibody.Conclusion:We conclude that PR is not associated with HHV-8 infection.展开更多
文摘0 引言。乙型肝炎病毒(HBV)属嗜肝DNA病毒属，是一组以侵袭肝脏为主的病毒，主要感染哺乳动物和鸟类．HBV DNA的长度为3．2kb，具有4个开放读码框架(ORF)，分别编码HBV的表面抗原蛋白，核心／e抗原蛋白，X蛋白以及HBV DNA多聚酶(HBV DNA P)．HBV DNA P基因在ORF中最长，并且与C、S、X基因区有重叠，其编码的P蛋白含有N-末端蛋白(TP)、逆转录酶(RT)／DNA多聚酶、RNase H和隔离片(spacer，SP)等4个结构域，各结构域分别位于2307—2840nt、133—1128nt、1129—1621nt和2841—0—132nt．目前对多聚酶及其所编码的各个结构域的研究取得了一定的进展．
文摘Background:Herpesvirus-like particles have been reported to be detectable by electron microscopy in lesional biopsy of patients with pityriasis rosea(PR).We report a study investigating the association of PR with human herpesvirus-8(HHV-8)infection.Methods:Our setting is a teaching clinic affiliated to a university.We recruited eight patients aged 28-47 years(mean:34.5 years)diagnosed with PR during a one-year period.We collected acute blood specimens at presentation and convalescent blood specimens three to four weeks later.We also collected skin scrapings from the herald patch where present and from truncal secondary lesions.Results:We detected HHV-8 DNA by a nested PCR(polymerase chain reaction)targeting,respectively,a 233-bp and a 160-bp fragment of ORF 26.PCR for HHV-8 DNA was negative in the peripheral blood mononuclear cells and plasma of acute and convalescent specimens of all patients,and negative in all skin scrapings.We detected anti-HHV-8 IgG and IgM antibodies by the indirect immunofluorescence.Four patients had IgG antibodies against HHV-8,but with no significant rise of titre.None were positive for anti-HHV-8 IgM antibody.Conclusion:We conclude that PR is not associated with HHV-8 infection.
