OBJECTIVE The EBNA1 IgA antibody level of normal and NPC subjects in a high incidence area were analyzed for new diagnostic criteria to improve diagnosis. METHODS A total of 780 normal and 104 NPC sera were tested for...OBJECTIVE The EBNA1 IgA antibody level of normal and NPC subjects in a high incidence area were analyzed for new diagnostic criteria to improve diagnosis. METHODS A total of 780 normal and 104 NPC sera were tested for EBNA1 IgA antibody levels by ELISA. Two diagnostic criteria were obtained from sensitivity and specificity data: 1) lower equivocal limit (rOD =1.10) where sensitivity = 95%; and 2) upper equivocal limit (rOD=1.85) where specificity = 95%. RESULTS The range and distribution of EBNA1 IgA antibody levels are broad with those of normal subjects (0.093-4.726, mean = 0.850 ± 0.637) overlapping those from NPC subjects (0.235-3.721, mean = 2.241 ± 0.875). However, NPC subjects did exhibit significantly higher antibody levels (t = 18.5, P<0.001). Based on the diagnostic criteria, 3 diagnostic categories were established: ① Positive; ② Suspected Positive; and 3) Negative. The percentage of NPC subjects falling into these 3 diagnostic categories were 75.13%, 17.44% and 7.44%, respectively and of normal subjects, 4.81%, 17.31%, 77.88% respectively. CONCLUSION Due to the broad distribution and overlapping of antibody levels between normal and NPC subjects in a high incidence area, it is important to have diagnostic criteria that will categorize those with equivocal results to minimize misdiagnosis. The 3 diagnostic categories established in this study will enhance detection and help physicians in their clinical diagnosis.展开更多
Epstein-Barr virus (EBV) infection is closely associated with nasopharyngeal carcinoma (NPC)and considered one of the major risk factors[1]. A limited number of viral proteins, such as latent membrane proteins (LMP1, ...Epstein-Barr virus (EBV) infection is closely associated with nasopharyngeal carcinoma (NPC)and considered one of the major risk factors[1]. A limited number of viral proteins, such as latent membrane proteins (LMP1, LMP2) and EB nuclear antigen1 (EBNA1), are expressed in NPC cells[2].Recognition epitopes for CD8+and CD4+T cells were included in the LMP2 antigen and EBNA1 C-terminal region (amino acid No. 380-641), respectively[3,4].Both CD4+and CD8+memory T-cell responses were efficiently reactivated by EBNA1 and LMP2[5;6].展开更多
文摘OBJECTIVE The EBNA1 IgA antibody level of normal and NPC subjects in a high incidence area were analyzed for new diagnostic criteria to improve diagnosis. METHODS A total of 780 normal and 104 NPC sera were tested for EBNA1 IgA antibody levels by ELISA. Two diagnostic criteria were obtained from sensitivity and specificity data: 1) lower equivocal limit (rOD =1.10) where sensitivity = 95%; and 2) upper equivocal limit (rOD=1.85) where specificity = 95%. RESULTS The range and distribution of EBNA1 IgA antibody levels are broad with those of normal subjects (0.093-4.726, mean = 0.850 ± 0.637) overlapping those from NPC subjects (0.235-3.721, mean = 2.241 ± 0.875). However, NPC subjects did exhibit significantly higher antibody levels (t = 18.5, P<0.001). Based on the diagnostic criteria, 3 diagnostic categories were established: ① Positive; ② Suspected Positive; and 3) Negative. The percentage of NPC subjects falling into these 3 diagnostic categories were 75.13%, 17.44% and 7.44%, respectively and of normal subjects, 4.81%, 17.31%, 77.88% respectively. CONCLUSION Due to the broad distribution and overlapping of antibody levels between normal and NPC subjects in a high incidence area, it is important to have diagnostic criteria that will categorize those with equivocal results to minimize misdiagnosis. The 3 diagnostic categories established in this study will enhance detection and help physicians in their clinical diagnosis.
基金supported by the Mega Project of Research on the Prevention and Control of HIV/AIDS,Viral Hepatitis Infectious Diseases [2018ZX10711001]
文摘Epstein-Barr virus (EBV) infection is closely associated with nasopharyngeal carcinoma (NPC)and considered one of the major risk factors[1]. A limited number of viral proteins, such as latent membrane proteins (LMP1, LMP2) and EB nuclear antigen1 (EBNA1), are expressed in NPC cells[2].Recognition epitopes for CD8+and CD4+T cells were included in the LMP2 antigen and EBNA1 C-terminal region (amino acid No. 380-641), respectively[3,4].Both CD4+and CD8+memory T-cell responses were efficiently reactivated by EBNA1 and LMP2[5;6].
