Objective: To examine if the EB virus appears innasopharyngeal epithelial cells in the course oftumorigenesis in nasopharyngeal carcinoma (NPC).Methods: Based on the annual investigation of SihuiCounty, a cancer-preve...Objective: To examine if the EB virus appears innasopharyngeal epithelial cells in the course oftumorigenesis in nasopharyngeal carcinoma (NPC).Methods: Based on the annual investigation of SihuiCounty, a cancer-prevention base of Sun Yat-senUniversity of Medical Sciences(SUMS) and armorHospital out-patient clinic, more than 450 paraffinembedded NPC tissue specimells, precancerous lesionsand precancerosis from a high--risk population werecollected for detection of an EBV-DNase gene fragmentby an optimized PCR method. Results: In 145 of the149 (97.3%) cases with invasive NPC, and 2 of 4 caseswith in situ NPC, only 2 of the 155 subjects withprecancerosis was DNase gene positive, and all 47 casesexamined in the out-patient department of the TumorHospital with precancerous lesions in the nasopharynxwere negative. Conclusion: The universal presence ofan EBV-DNase gene in NPC tissues and the paucity ofEBV-DNase gene in nonmalignant and precancerouslesions in the nasopharynx in a high-risk populationimply that carcinogenesis of NPC occurs before theappearance of EBV in the nasopharynx.展开更多
In 1975, it was found that the HMPA could induce the nasal tumors in the rats, the carcinogenicity and the Iatency were dose correlated with the HMPA concentration. it was estimated by the National Institute for occu...In 1975, it was found that the HMPA could induce the nasal tumors in the rats, the carcinogenicity and the Iatency were dose correlated with the HMPA concentration. it was estimated by the National Institute for occupation Safety Health that about 5,000 People were occupationally exposed to HMPA in 1975, and more than 90% exposures are in research laboralories. Since then,no one patient suffering from NPC or nasal tumors has been found. In 1987, in a mass survey of Antibodies to Epstein-Barr Virus specific DNase (EDAb). Authors found that there were eight persons who had a long term contact with HMPA (8-10 years) in 1970-1980 in Guangrhou Chemical Engineering Institute. 2 of the 8 were EDAb positive (high titre) but the nasopharyngeal cavity were not found any abnormal. Owing to the history of contact with HMPA and a vigorous elevation of EDAb titre in the duration of 2.5 yeas' follow up was observed. There 2 persons were considered as a high risk population of NPC and a 'Blind Biopsy' of nasopharynx was suggested. A diagnosis of poorly differentiated squamous carcinoma confirmed pathologically.of these 2 Patients were stage 1(TINOMO) and were treated with radiotherapy and live very well till now. The results suggested that the HMPA might induced human nasopharyngeal carcinoma.展开更多
文摘Objective: To examine if the EB virus appears innasopharyngeal epithelial cells in the course oftumorigenesis in nasopharyngeal carcinoma (NPC).Methods: Based on the annual investigation of SihuiCounty, a cancer-prevention base of Sun Yat-senUniversity of Medical Sciences(SUMS) and armorHospital out-patient clinic, more than 450 paraffinembedded NPC tissue specimells, precancerous lesionsand precancerosis from a high--risk population werecollected for detection of an EBV-DNase gene fragmentby an optimized PCR method. Results: In 145 of the149 (97.3%) cases with invasive NPC, and 2 of 4 caseswith in situ NPC, only 2 of the 155 subjects withprecancerosis was DNase gene positive, and all 47 casesexamined in the out-patient department of the TumorHospital with precancerous lesions in the nasopharynxwere negative. Conclusion: The universal presence ofan EBV-DNase gene in NPC tissues and the paucity ofEBV-DNase gene in nonmalignant and precancerouslesions in the nasopharynx in a high-risk populationimply that carcinogenesis of NPC occurs before theappearance of EBV in the nasopharynx.
文摘In 1975, it was found that the HMPA could induce the nasal tumors in the rats, the carcinogenicity and the Iatency were dose correlated with the HMPA concentration. it was estimated by the National Institute for occupation Safety Health that about 5,000 People were occupationally exposed to HMPA in 1975, and more than 90% exposures are in research laboralories. Since then,no one patient suffering from NPC or nasal tumors has been found. In 1987, in a mass survey of Antibodies to Epstein-Barr Virus specific DNase (EDAb). Authors found that there were eight persons who had a long term contact with HMPA (8-10 years) in 1970-1980 in Guangrhou Chemical Engineering Institute. 2 of the 8 were EDAb positive (high titre) but the nasopharyngeal cavity were not found any abnormal. Owing to the history of contact with HMPA and a vigorous elevation of EDAb titre in the duration of 2.5 yeas' follow up was observed. There 2 persons were considered as a high risk population of NPC and a 'Blind Biopsy' of nasopharynx was suggested. A diagnosis of poorly differentiated squamous carcinoma confirmed pathologically.of these 2 Patients were stage 1(TINOMO) and were treated with radiotherapy and live very well till now. The results suggested that the HMPA might induced human nasopharyngeal carcinoma.
