<p style="margin-left:10.0pt;"> <span><span>Steroidal plant growth promoters (SPGP) have been continuously studied due to their high activity increasing biomass and resistance to diverse st...<p style="margin-left:10.0pt;"> <span><span>Steroidal plant growth promoters (SPGP) have been continuously studied due to their high activity increasing biomass and resistance to diverse stress fact</span><span>ors. In our hands, a new SPGP family of 22-oxocholestanic compou</span><span>nds stands out at a comparative level to brassinosteroids (BSs). The potential activity of new SPGP against phytopathogens was studied through </span><i><span>in silico</span></i><span> molecular docking, these assays were performed with relevant ensymes of phytopatogens Chitinase B and 1,3-</span></span><i><span>β</span></i><span>-Glucanase. Nine Chitinase B inhibitors and two 1,3-</span><i><span>β</span></i><span>-Glucanase inhibitors were proposed. The launched study analyzed the interactional and spatial level, determining the presence of interactions with key</span><span> </span><span>amino acid</span><span>s</span><span> in receptors in comparison to reference inhibitors. Even more, the AVR4 and ECP6 effectors were also examined. No compound that blocks ECP6 was found;due to, probably, the influence of its highly hydrophilic environment. In the case of AVR4, two SPGP showed a better docking score (DS) than a chitin fragment (endogenous ligand);this fact demonstrates the latent potential of the 22-oxocholestanic derivatives against phytopathogens, with a specific regulation via proliferation inhibition. Moreover, this SPGP do</span><span>es</span><span> not affect the symbiotic fungi that are beneficial for the natural plant system.</span> </p>展开更多
文摘<p style="margin-left:10.0pt;"> <span><span>Steroidal plant growth promoters (SPGP) have been continuously studied due to their high activity increasing biomass and resistance to diverse stress fact</span><span>ors. In our hands, a new SPGP family of 22-oxocholestanic compou</span><span>nds stands out at a comparative level to brassinosteroids (BSs). The potential activity of new SPGP against phytopathogens was studied through </span><i><span>in silico</span></i><span> molecular docking, these assays were performed with relevant ensymes of phytopatogens Chitinase B and 1,3-</span></span><i><span>β</span></i><span>-Glucanase. Nine Chitinase B inhibitors and two 1,3-</span><i><span>β</span></i><span>-Glucanase inhibitors were proposed. The launched study analyzed the interactional and spatial level, determining the presence of interactions with key</span><span> </span><span>amino acid</span><span>s</span><span> in receptors in comparison to reference inhibitors. Even more, the AVR4 and ECP6 effectors were also examined. No compound that blocks ECP6 was found;due to, probably, the influence of its highly hydrophilic environment. In the case of AVR4, two SPGP showed a better docking score (DS) than a chitin fragment (endogenous ligand);this fact demonstrates the latent potential of the 22-oxocholestanic derivatives against phytopathogens, with a specific regulation via proliferation inhibition. Moreover, this SPGP do</span><span>es</span><span> not affect the symbiotic fungi that are beneficial for the natural plant system.</span> </p>