The optimum conditions and effectiveness of extraction of extracellular polymeric substances (EPS) from activated sludge in a membrane bioreactor(MBR) were studied under such conditions, thermal, acid, alkaline treatm...The optimum conditions and effectiveness of extraction of extracellular polymeric substances (EPS) from activated sludge in a membrane bioreactor(MBR) were studied under such conditions, thermal, acid, alkaline treatment methods, and it was concluded that both thermal treatment (80℃,45 min) and EDTA bi-sodium treatment were effective.The sludge cell was damaged seriously by NaOH treatment (1 mol·L-1,2 h), but when formaldehyde was added, the nucleic acid concentration was reduced by 21.5%, and the damage to sludge cell was then decreased.展开更多
Regardless of thermodynamic stability, less complex proteome than plasma, convenient and noninvasive sampling of urine, reproducibility and comparability of the data among laboratories are challenges for the urine pro...Regardless of thermodynamic stability, less complex proteome than plasma, convenient and noninvasive sampling of urine, reproducibility and comparability of the data among laboratories are challenges for the urine proteome analysis. Pretreatment of urine with 10 mM EDTA in conjunction with desalting methods to remove interfering molecules significantly improved the presentation of urine proteome on 2-D gels. Statistical analysis of the average ratio of the spot numbers on 2-D gels between EDTA-treated and non-treated samples were 1.35 ± 0.167, 1.26 ± 0.091, and 1.24 ± 0.095 for dialysis, ultrafiltration, and desalting column, respectively, with the p-value of 5.85 × 10–6 for the overall comparison. This result came from the fact that EDTA-treated samples showed better resolutions with less streaks and clearer spots on 2-D gels than the control samples. The results suggest that a simple and non-fractionating EDTA-treatment can be employed to the procedures for the high resolution 2-D gel analysis and other urinary proteomic analysis.展开更多
使用介质阻挡放电和脉冲电晕放电2种低温等离子体对电厂锅炉乙二胺四乙酸二钠(EDTA)清洗废水进行处理,采用COD降解率、色度去除率为评价指标,探寻放电功率、电介质间距(介质阻挡放电)/电极间距(脉冲电晕放电)、放电时间、处理量等因素对...使用介质阻挡放电和脉冲电晕放电2种低温等离子体对电厂锅炉乙二胺四乙酸二钠(EDTA)清洗废水进行处理,采用COD降解率、色度去除率为评价指标,探寻放电功率、电介质间距(介质阻挡放电)/电极间距(脉冲电晕放电)、放电时间、处理量等因素对COD降解率及色度去除率性能的影响。结果表明,当放电功率为15 k W,电介质间距/电极间距为5 mm时,2 h可使电厂锅炉EDTA清洗废水COD降解率70%以上、色度去除率90%以上。展开更多
文摘The optimum conditions and effectiveness of extraction of extracellular polymeric substances (EPS) from activated sludge in a membrane bioreactor(MBR) were studied under such conditions, thermal, acid, alkaline treatment methods, and it was concluded that both thermal treatment (80℃,45 min) and EDTA bi-sodium treatment were effective.The sludge cell was damaged seriously by NaOH treatment (1 mol·L-1,2 h), but when formaldehyde was added, the nucleic acid concentration was reduced by 21.5%, and the damage to sludge cell was then decreased.
文摘Regardless of thermodynamic stability, less complex proteome than plasma, convenient and noninvasive sampling of urine, reproducibility and comparability of the data among laboratories are challenges for the urine proteome analysis. Pretreatment of urine with 10 mM EDTA in conjunction with desalting methods to remove interfering molecules significantly improved the presentation of urine proteome on 2-D gels. Statistical analysis of the average ratio of the spot numbers on 2-D gels between EDTA-treated and non-treated samples were 1.35 ± 0.167, 1.26 ± 0.091, and 1.24 ± 0.095 for dialysis, ultrafiltration, and desalting column, respectively, with the p-value of 5.85 × 10–6 for the overall comparison. This result came from the fact that EDTA-treated samples showed better resolutions with less streaks and clearer spots on 2-D gels than the control samples. The results suggest that a simple and non-fractionating EDTA-treatment can be employed to the procedures for the high resolution 2-D gel analysis and other urinary proteomic analysis.
文摘使用介质阻挡放电和脉冲电晕放电2种低温等离子体对电厂锅炉乙二胺四乙酸二钠(EDTA)清洗废水进行处理,采用COD降解率、色度去除率为评价指标,探寻放电功率、电介质间距(介质阻挡放电)/电极间距(脉冲电晕放电)、放电时间、处理量等因素对COD降解率及色度去除率性能的影响。结果表明,当放电功率为15 k W,电介质间距/电极间距为5 mm时,2 h可使电厂锅炉EDTA清洗废水COD降解率70%以上、色度去除率90%以上。
