As a part of a basic research project on Xeno-transplantion. we have been engaged in the derivation of embryonic stein cell lines from Chinese mini swine. Here, we reported for the first time the establishment of two ...As a part of a basic research project on Xeno-transplantion. we have been engaged in the derivation of embryonic stein cell lines from Chinese mini swine. Here, we reported for the first time the establishment of two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 and a 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, marker characterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentially useful for further basic studies.展开更多
基金an introductory part of a project supported by National Science Foundation of China(No.39993430).
文摘As a part of a basic research project on Xeno-transplantion. we have been engaged in the derivation of embryonic stein cell lines from Chinese mini swine. Here, we reported for the first time the establishment of two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 and a 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, marker characterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentially useful for further basic studies.