The specific recombinant proteins rOspA B. afzelii, rOspA B. garinii a rOspA B. burgdorferi sensu stricto were used as antigens for construction of ELISA sets. ELISA examination enables determination of specific post-...The specific recombinant proteins rOspA B. afzelii, rOspA B. garinii a rOspA B. burgdorferi sensu stricto were used as antigens for construction of ELISA sets. ELISA examination enables determination of specific post-vaccination antibodies against OspA B. afzelii, B. garinii a B. burgdorferi sensu stricto.Using recombinant DNA technology, genes from B. afzelii, B. garinii and B. burgdorferi sensu stricto were inserted into E. coli-expression vectors and the rOspA proteins were produced. These proteins were used for the construction of ELISA kits for the determination of post-vaccination antibodies against individual Borrelia serovars contained in the vaccine. The antibody response of dogs vaccinated with whole-cell vaccine BORRELYM 3 and non-vaccinated dogs was monitored and compared. The ELISA method proved as highly sensitive for the determination of post-vaccination antibodies against individual Borrelia serovars in vaccinated animals. Detection of these antibodies and their quantification may be used for evaluation of efficiency of vaccines against Lyme borreliosis in dogs caused by Borrelia burgdorferi sensu lato. Prospectively, it will be necessary to establish a correlation between post-vaccination antibody levels and protective immunity of vaccinated dogs.展开更多
[Objective] The aim of this study was to study on expression of Bt protein in transgenic pest-resistant rice. [Method] Enzyme-linked immunosorbent assay （ELISA） was used to measure Bt protein expression in different...[Objective] The aim of this study was to study on expression of Bt protein in transgenic pest-resistant rice. [Method] Enzyme-linked immunosorbent assay （ELISA） was used to measure Bt protein expression in different tissues of transgenic pest-resistant rice at same growth stage. [Result] Absolute content of Bt protein from high to low was as follows： leaves 〉 immature seeds and glumes 〉 roots 〉 stems in different tissues of transgenic rice in grain-filling stage; Bt protein content of trans- genic rice changed a little in different growth stages （including tillering stage, booting stage, and grain-filling stage）; in general, its level declined a little in later growth stage, but the resistibility would not be influenced significantly. [Conclusion] The ex- periment is significant for pest prevention and transgenic rice breeding.展开更多
A novel monoclonal antibody （MAb） against oxytetracycline （OTC） was generated and characterized. The MAb was used in the development of an enzyme-linked immunosorbant assay （ELISA）-based detection system. An OTC...A novel monoclonal antibody （MAb） against oxytetracycline （OTC） was generated and characterized. The MAb was used in the development of an enzyme-linked immunosorbant assay （ELISA）-based detection system. An OTC-bovine serum albumin （BSA） conjugate was prepared and used in the immunization of mice. A conventional somatic cell fusion technique was used to generate MAb-secreting hybridomas denoted 2-4F, 7-3G, and 11-11A. An indirect competitive ELISA （icELISA） was applied to measure the sensitivity and specificity of each MAb in terms of its 50% inhibitory concentration （IC50） and percentage of cross-reactivity, respectively. MAb 2-4F exhibited the highest sensitivity, with an ICs0 of 7.01 ng/ml. This MAb showed strong cross-reactivity to rolitetracycline, but no cross- reactivity to other unrelated antibiotics. When MAb 2-4F was used to detect OTC from shrimp samples, the recoveries were in the range of 82%-118% for an intra-assay and 96%-113% for an inter-assay. The coefficients of variation of the assays were 3.9%-13.9% and 5.5%-14.9%, respectively.展开更多
文摘The specific recombinant proteins rOspA B. afzelii, rOspA B. garinii a rOspA B. burgdorferi sensu stricto were used as antigens for construction of ELISA sets. ELISA examination enables determination of specific post-vaccination antibodies against OspA B. afzelii, B. garinii a B. burgdorferi sensu stricto.Using recombinant DNA technology, genes from B. afzelii, B. garinii and B. burgdorferi sensu stricto were inserted into E. coli-expression vectors and the rOspA proteins were produced. These proteins were used for the construction of ELISA kits for the determination of post-vaccination antibodies against individual Borrelia serovars contained in the vaccine. The antibody response of dogs vaccinated with whole-cell vaccine BORRELYM 3 and non-vaccinated dogs was monitored and compared. The ELISA method proved as highly sensitive for the determination of post-vaccination antibodies against individual Borrelia serovars in vaccinated animals. Detection of these antibodies and their quantification may be used for evaluation of efficiency of vaccines against Lyme borreliosis in dogs caused by Borrelia burgdorferi sensu lato. Prospectively, it will be necessary to establish a correlation between post-vaccination antibody levels and protective immunity of vaccinated dogs.
基金Transgenic cry1C Novel Materials of Japonica Rice Resistant Against Snout Moth’s Larva Cultivated with Biotechnology (201205068)The National Program of Transgenic Variety Development of China (2011ZX08001-001)~~
文摘[Objective] The aim of this study was to study on expression of Bt protein in transgenic pest-resistant rice. [Method] Enzyme-linked immunosorbent assay （ELISA） was used to measure Bt protein expression in different tissues of transgenic pest-resistant rice at same growth stage. [Result] Absolute content of Bt protein from high to low was as follows： leaves 〉 immature seeds and glumes 〉 roots 〉 stems in different tissues of transgenic rice in grain-filling stage; Bt protein content of trans- genic rice changed a little in different growth stages （including tillering stage, booting stage, and grain-filling stage）; in general, its level declined a little in later growth stage, but the resistibility would not be influenced significantly. [Conclusion] The ex- periment is significant for pest prevention and transgenic rice breeding.
基金supported by the Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University(No.RD_50-53_61)the National Research University Development Project(No.AM1023A)the Office of the Higher Education Commission,Thailand
文摘A novel monoclonal antibody （MAb） against oxytetracycline （OTC） was generated and characterized. The MAb was used in the development of an enzyme-linked immunosorbant assay （ELISA）-based detection system. An OTC-bovine serum albumin （BSA） conjugate was prepared and used in the immunization of mice. A conventional somatic cell fusion technique was used to generate MAb-secreting hybridomas denoted 2-4F, 7-3G, and 11-11A. An indirect competitive ELISA （icELISA） was applied to measure the sensitivity and specificity of each MAb in terms of its 50% inhibitory concentration （IC50） and percentage of cross-reactivity, respectively. MAb 2-4F exhibited the highest sensitivity, with an ICs0 of 7.01 ng/ml. This MAb showed strong cross-reactivity to rolitetracycline, but no cross- reactivity to other unrelated antibiotics. When MAb 2-4F was used to detect OTC from shrimp samples, the recoveries were in the range of 82%-118% for an intra-assay and 96%-113% for an inter-assay. The coefficients of variation of the assays were 3.9%-13.9% and 5.5%-14.9%, respectively.
