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EMA-PCR检测金黄色葡萄球菌活菌的方法 被引量:4
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作者 赵素君 李江凌 +6 位作者 谢晶 曹冶 王秋实 叶勇刚 罗丹丹 叶健强 廖党金 《中国兽医杂志》 CAS 北大核心 2013年第3期18-20,共3页
根据金黄色葡萄球菌(SA)耐热核酸酶编码基因(nuc基因)设计特异性引物,对试验菌株进行PCR检测,结果显示,金黄色葡萄球菌扩增出大小为480bp的特异性条带,而其他对照菌株未扩增出条带。由于传统PCR技术无法区分样品中的死细菌与活细菌,从... 根据金黄色葡萄球菌(SA)耐热核酸酶编码基因(nuc基因)设计特异性引物,对试验菌株进行PCR检测,结果显示,金黄色葡萄球菌扩增出大小为480bp的特异性条带,而其他对照菌株未扩增出条带。由于传统PCR技术无法区分样品中的死细菌与活细菌,从而使检测结果往往出现很高的假阳性。本试验利用EMA能穿过死细菌的细胞膜并在光激活的作用下能与基因组DNA共价结合,从而能抑制死菌DNA进行PCR扩增的特性,建立了一种快速、有效的检测金黄色葡萄球菌活菌的EMA-PCR方法,较传统PCR大大提高了检测的准确性和可靠性,该方法检测灵敏度可达15CFU/mL。 展开更多
关键词 金黄色葡萄球菌 flue基因 ema—PCR 死细菌 活细菌
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The Impact of the Environment Management Act (EMA) on the Accountability of Companies in Fiji
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作者 Glen Finau Jale Samuwai Masilina Rotuivaqali Clayton Kuma Lusiana Kanaenabogi Tevita Veituna 《Journal of Modern Accounting and Auditing》 2013年第9期1216-1234,共19页
This study seeks to explore the impact of the Environment Management Act (EMA) (2005) on the accountability of three companies in Fiji. The study uses a multi-case study approach based on three subsidiaries of a c... This study seeks to explore the impact of the Environment Management Act (EMA) (2005) on the accountability of three companies in Fiji. The study uses a multi-case study approach based on three subsidiaries of a conglomerate. Data collection methods include semi-structured interviews with accountants, internal auditors, and environmental officers, document reviews, and content analysis of annual reports and websites of the respective companies. The findings suggest that the EMA (2005) had some effects in terms of engendering accountability on the companies studied. Further evidence suggests that while this increased accountability has led to disclosures in annual reports and websites, these disclosures are minimal at best; furthermore, they primarily address that the stakeholder group comprised government regulatory authorities. The study provides policy implications on how environmental legislations could be designed to improve the accountability of commercial entities in developing economies. The experience and issues highlighted are also useful to other developing economies who are contemplating in developing their own environmental legislations. This paper is one of the few papers that explore the impact of environmental legislations on accountability in a developing economy context. 展开更多
关键词 environmental legislation Environment Management Act ema sustainability developing economy ACCOUNTABILITY
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温度对金属/绝缘电介质复合物介电函数的影响
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作者 谢秉川 《铁道师院学报》 2001年第3期43-47,共5页
采用两种最常用的近似方法 (MGT和EMA)研究了金属 /绝缘电介质复合材料有效介电函数随温度变化的规律 ,不同的方法描述的结果是不同的 ;MGT方法得到的介电函数实部始终随温度单调递增 ;而EMA方法得到的介电函数实部随温度增加或减小 ,... 采用两种最常用的近似方法 (MGT和EMA)研究了金属 /绝缘电介质复合材料有效介电函数随温度变化的规律 ,不同的方法描述的结果是不同的 ;MGT方法得到的介电函数实部始终随温度单调递增 ;而EMA方法得到的介电函数实部随温度增加或减小 ,同时两者的数值也相差较大 。 展开更多
关键词 介电函数 温度效应 金属/绝缘电介质复合材料 MGT ema法 电学性质 光学性质
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