By the methods of quantitative cytophotometry, we have identified the changes in the nucleus and of some intranuclear compartments in the early stages of infection with encephalomyocarditis virus (EMCV). They can be c...By the methods of quantitative cytophotometry, we have identified the changes in the nucleus and of some intranuclear compartments in the early stages of infection with encephalomyocarditis virus (EMCV). They can be characterized as early 1 - 2 hours post infection (hpi) and temporary increase (duration about 1 hour) in the content of the acidic proteins of the nucleolus, changing their decline to the control values. Then (after 1 - 2 hours) follows an increase in RNA content of nucleoli to 4 hours post infection (the process takes about 2 hours). The increase in RNA content in nucleoli is in approximately the same time (slightly behind) with the activation of PML bodies (2 - 4 hpi). Then, the RNA content in nucleoli decreased to the control values, while simultaneously decreasing activity of PML bodies (ranging from 5 - 6 hpi). The early stages of infection EMCV are also characterized by the tendency to increase in the size of the nuclei of infected cells, and preserve at a later time. Then there is an increase in RNA content in the nucleus, roughly coinciding with the increased content of RNA in the nucleoli.展开更多
文摘By the methods of quantitative cytophotometry, we have identified the changes in the nucleus and of some intranuclear compartments in the early stages of infection with encephalomyocarditis virus (EMCV). They can be characterized as early 1 - 2 hours post infection (hpi) and temporary increase (duration about 1 hour) in the content of the acidic proteins of the nucleolus, changing their decline to the control values. Then (after 1 - 2 hours) follows an increase in RNA content of nucleoli to 4 hours post infection (the process takes about 2 hours). The increase in RNA content in nucleoli is in approximately the same time (slightly behind) with the activation of PML bodies (2 - 4 hpi). Then, the RNA content in nucleoli decreased to the control values, while simultaneously decreasing activity of PML bodies (ranging from 5 - 6 hpi). The early stages of infection EMCV are also characterized by the tendency to increase in the size of the nuclei of infected cells, and preserve at a later time. Then there is an increase in RNA content in the nucleus, roughly coinciding with the increased content of RNA in the nucleoli.
文摘【目的】利用网络药理学和分子对接技术发现莪术醇抗脑心肌炎病毒(Encephalomyocarditis virus,EMCV)的作用靶点及机制。【方法】利用PharmMapper、GeneCards数据库获得莪术醇抗EMCV的相关靶点;通过Cytoscape 3.7.2软件、STRING和DAVID数据库构建靶蛋白互作(PPI)网络并筛选关键靶点,对靶点进行GO功能和KEGG通路富集分析,并构建莪术醇抗EMCV靶点-通路网络;通过AutoDock Vina 1.1.2分析莪术醇与靶蛋白的结合能及结合模式。【结果】网络药理学分析结果显示,莪术醇抗EMCV的潜在靶点有9个,其中丝裂原活化激酶14(mitogen-activated protein kinase 14,MAPK14)、信号转导与转录激活因子1(signal transducer and activator of transcription 1,STAT1)、白蛋白(albumin,ALB)、白细胞介素2(interleukin 2,IL2)可能是莪术醇抗EMCV的核心靶点,所得靶点参与C型凝集素受体信号通路、神经营养素信号通路和JAK-STAT信号通路等代谢通路,功能涉及调节炎症反应细胞因子的产生、蛋白激酶活性和药物结合等;分子对接结果显示,4种核心靶蛋白与莪术醇之间存在较强的结合能,均存在疏水形式的结合,其中ALB、STAT1和IL2与莪术醇之间还存在氢键结合。【结论】本研究结果表明,MAPK14、STAT1、ALB和IL2是莪术醇发挥抗EMCV作用的潜在靶点,本研究为莪术醇作为抗EMCV药物的研发提供理论依据和线索。
