EMP-1 Promotes Tumorigenesis of NSCLC through PI3K/AKT Pathway

This study examined the role of EMP-1 in tumorigenesis of non-small cell lung carcinoma (NSCLC) and the possible mechanism. Specimens were collected from 28 patients with benign lung diseases and 28 with NSCLC, and immunohis to chemically detected to evaluate the correlation of EMP-1 expression to the clinical features of NSCLC. Recombinant adenovirus was constructed to over-express EMP-1 and then infect PC9 cells. Cell proliferation was measured by Ki67 staining. Western blotting was performed to examine the effect of EMP-1 on the PI3K/AKT signaling. Moreover, tumor xeno-grafts were established by subcutaneous injection of PC9 cell suspension (about 5×107/mL in 100 μL of PBS) into the right hind limbs of athymic nude mice. The results showed EMP-1 was significantly up-regulated in NSCLC patients as compared with those with benign lung diseases. Over-expression of EMP-1 promoted proliferation of PC9 cells, which coincided with the activation of the PI3K/AKT pathway. EMP-1 promoted the growth of xenografts of PC9 cells in athymic nude mice. It was concluded that EMP-1 expression may contribute to the development and progress of NSCLC by activating PI3K/AKT pathway.

EMP-1基因对人食管癌细胞系生长的影响

背景与目的:本室曾用正常食管粘膜上皮细胞和食管癌细胞进行微阵列分析,得到一批在正常食管粘膜上皮细胞和食管癌细胞中差异表达的基因,其中包括上皮膜蛋白-1(EMP-1)基因,EMP-1基因的mRNA水平表达差异有6倍。本文进一步研究EMP-1基因在食管癌发生发展中的作用;方法:构建人EMP-1基因的真核表达载体,并转染人食管癌细胞系,Westernblot检测转染效果,RT-PCR检测转染后EMP-1的表达情况,绘制生长曲线,流式细胞检测细胞周期的变化;结果:EMP-1基因成功转入人食管癌细胞系EC9706,EMP-1基因表达量变高,细胞生长变慢,S期减少;结论:EMP-1基因与食管癌发生有一定关系。