Effective extraction of polyribosomes from astrocytes enables future discoveries on translation regulation

Translation regulation is an important layer of gene expression:Generation of genome-wide expression datasets at multi-omics levels in spatial,temporal,and cell-type resolution is essential for deciphering brain complexity.Regulation of gene expression is a highly dynamic process aiming at the production of precise levels of gene products to guarantee optimal cellular function,in response to physiological cues.Speedy advances in next-generation sequencing enabled the understanding of epigenomic and transcriptomic dynamic landscapes of different brain regions along development,aging,and disease progression.However,the correlation of the“transcriptome”with protein levels is poor because numerous mRNAs are subjected to manipulation of their translation efficiency,to warrant a favorable result under certain conditions.Hence,it is widely accepted that regulation at the translation level is a vital layer of gene expression.Quantification of actively translated mRNA populations(i.e.,“translatome”)is a more reliable predictor of the“proteome”(Wang et al.,2020).

A magic organic molecule assembled capping layer enables airprocessed α-FAPbI_(3) perovskite solar cell with state-of-the-art performances

The black-phase formamidine-lead iodide(α-FAPbI_(3)),boasting an optimal bandgap of 1.5 eV,stands out as a premier choice for narrow-bandgap perovskite solar cells(PSCs),achieving a certified power conversion efficiency(PCE)of 26.1%[1−5].This impressive performance hinges on the orderly and homogeneous crystallization ofα-phase pure FAPbI_(3),facilitated by coordinating solvents such as dimethyl sulfoxide(DMSO)to form intermediates like PbI_(2)-DMSO complex(D-complex).The D-complex plays a pivotal role in crystallization thermodynamics,enabling the direct formation of α-FAPbI_(3) without the photoinactiveδ-phase[6−9].However,DMSO,a commonly used coordinating solvent,is highly hygroscopic and prone to hydration upon moisture exposure.This tendency leads to incomplete perovskite crystallization and accelerates the transformation of α-FAPbI_(3) into itsδ-phase[2,10].Consequently,the best-performing α-FAPbI_(3)PSCs must be processed in an inert atmosphere with strictly controlled relative humidity(RH)and suffers from relatively poor reproducibility.Given the hard-to-control atmosphere at industrial scale,it is challenging yet imperative to eliminate the negative effects stemming from hygroscopic coordinating solvents[11−13].

Inspires effective alternatives to backpropagation:predictive coding helps understand and build learning

Artificial neural networks are capable of machine learning by simulating the hiera rchical structure of the human brain.To enable learning by brain and machine,it is essential to accurately identify and correct the prediction errors,referred to as credit assignment(Lillicrap et al.,2020).It is critical to develop artificial intelligence by understanding how the brain deals with credit assignment in neuroscience.

基于XML Enabled数据库技术的应用研究

利用XMLEnabled数据库DB2XMLAPI技术可以实现传统数据库向XML数据的动态转换,并在此基础上对生成的XML文档进行查询和显示。这种方法在保留了现有数据的基础上,减少了数据库读取次数,又满足了XML应用的需求,可作为一种面向B2CXML应用的通用方法。

电信产品运营管理的ENABLE模型和应用探讨

基于ENABLE模型的产品运营管理,就是在产品生命周期管理的基础上,通过探索新场景、开发新功能、采取新模式,促使产品升级,满足用户"求新"需求,也是通过提高产品价值感知、优化用户体验,提升用户满意度,满足用户"求好"需求。

Fano Resonance Enabled Infrared Nano-Imaging of Local Strain in Bilayer Graphene

Detection of local strain at the nanometer scale with high sensitivity remains challenging.Here we report near-field infrared nano-imaging of local strains in bilayer graphene by probing strain-induced shifts of phonon frequency.As a non-polar crystal,intrinsic bilayer graphene possesses little infrared response at its transverse optical phonon frequency.The reported optical detection of local strain is enabled by applying a vertical electrical field that breaks the symmetry of the two graphene layers and introduces finite electrical dipole moment to graphene phonon.The activated phonon further interacts with continuum electronic transitions,and generates a strong Fano resonance.The resulted Fano resonance features a very sharp near-field infrared scattering peak,which leads to an extraordinary sensitivity of-0.002%for the strain detection.Our results demonstrate the first nano-scale near-field Fano resonance,provide a new way to probe local strains with high sensitivity in non-polar crystals,and open exciting possibilities for studying strain-induced rich phenomena.

Exploring Enablers and Barriers to Municipal Solid Waste (MSW) Management Technologies Adoption in the Kingdom of Bahrain

Municipal Solid Waste (MSW) becomes a point of focus globally due to its harmful effects on the environment and human health if it is not managed properly. MSW was proved globally to be used as a resource, and it has a major opportunity in the realm of conversion technologies. Accordingly, this study aims to explore the enablers and barriers to six different MSW management (MSWM) technologies adopted in the Kingdom of Bahrain, in order to ensure the successful adoption of these technologies that are Anaerobic Digestion (AD), Incineration, Pyrolysis, Gasification, Composting and Refused Derived Fuel (RDF). This study provides sufficient information to the decision and policy-makers for the processes of selection and adoption of the MSWM technologies in Bahrain, using a qualitative approach “mainly semi-structured interviews” with experts and then Thematic Analysis using nvivo12 software. The results show that the main themes that enablers and barriers fall under are: political (e.g. national waste management strategy), technical (e.g. segregation at source), managerial (e.g. capacity building), social (e.g. public awareness), economic (e.g. incentives to investment) and environmental (e.g. air quality). This study concluded that in order to succeed in the MSWM technologies adoption, the resulted barriers should be overcome. Furthermore, Incineration was recommended as the best solution to manage MSW which has the least barriers and most enablers in Bahrain as per the experts.

Systems Immunology Enabled Immune Engineering

The immune checkpoint blockade has revolutionized cancer treatment.However,not all cancer types are susceptible to this therapy.Even in melanoma,one of the best scenario,about half of the patients do not respond to immune checkpoint blockade.Since CD8+T cell is the main driving force behind cancer elimination,then having a complete and competent T cell repertoire to cover all possible cancer antigens expressed by cancer cells should be a determining factor to the success of this therapy.Conversely,if there are'holes'in patients’T cell repertoire and/or'weak spots'manifested as functional dysregulation or exhaustion on T cells specific to a set of cancer antigens that dominantly expressed by cancer cells,cancer immune escape is inevitable.However,these two types of cancer immune escape might need different treatment strategies:the first group with'holes'in the T cell repertoire,whether the'holes'are taking on a form of missing T cells to cover these cancer antigens or missing high-affinity TCRs that are known to be more sensitive to antigen stimulation,would be benefited from TCR re-directed adoptive cell transfer(ACT)therapy;the other group with T cell repertoire'weak spots'would be benefited from immune checkpoint blockade alone or in combination with additional stimulatory factors such as cytokines and peptide vaccine.In the past decade,we have developed several tools to profile the T cell repertoire from T cell receptor diversity to T cell receptor affinity to high-throughput linking antigen specificity to single T cell receptor sequences in large scale.In this talk,I will first introduce these tools and then give examples on how we use them to answer some of the fundamental questions in systems immunology with a focus on cancer immunology,which in turn help us design new therapeutics immune engineering.

A patterned titania nanorod array enables high fill factor in perovskite solar cells

Perovskite solar cells (PSCs) have emerged as a new-generation photovoltaic technology that features both low manufacturing cost and high power conversion efficiencies (PCEs) [1-3].In the past decade,the PCEs of PSCs have increased from 3.8% to 25.5%,which are now comparable to those of silicon-based solar cells.Nevertheless,by comparing the detailed device parameters of record PSCs reported at different times,Peng et al.

Correction to:An endotenon sheath‑inspired double‑network binder enables superior cycling performance of silicon electrodes

The original version of this article unfortunately contained some mistakes.1.The authors found that the data unit in Fig.3a–f is wrong.The corrected version of Fig.3 is given below:2.The authors found that explanation of the data lines in Fig.2e is wrong.The corrected version of the explanation of Fig.2e is given below:The DNB can endure approximately 300%stretching and withstand stress up to about 1.5 MPa,as shown in Fig.2e.

Next Generation Network Enabled by ZTE's Softswitch Technology

Softswitch technology integrates the su-periorities of both an intelligence net-work and the Internet, which embodiesits maturity and advancement. With ahierarchical network model, it effectivelysolves problems of evolution and convergenceof current communication networks. It also fol-

Biologically Enabled Syntheses of Nanostructured 3-D Sensor Materials:the Potential for 3-D Genetically Engineered Microdevices (3-D GEMs)

Three-dimensional (3-D)self-assembly of nanos- tructures and nanodevices on a large scale remains a grand quest for mankind.Freestanding nanostructured assemblies with controlled 3-D shapes can exhibit attractive properties for sensor and other applications. Protocols for 3-D self-assembly that can be scaled up for mass production on a large up to tonnage)scale, while preserving morphological features on a small (down to nanometer)scale,are needed to allow for widespread use of 3-D nanostructures in advanced devices.However,these often conflicting requirements of scalability and precision pose a difficult challenge for synthetic (man-made)processing routes.

Patient Enablement in Chronic Diseases in Primary Health Care, Riyadh City, KSA

Background: Chronic diseases continue to cause high morbidity and mortality in Saudi Arabia. Patients severing from diabetes mellitus, hypertension and associated complications have recently increased and most of these patients find it extremely difficult to understand or cope with their illness. The objective of this study is to determine the level of patients’ enablement in chronic disease and its predictors. Methods: A community based cross-sectional study was conducted between December 2014 and January 2015. Six hundred and four (604) Patients attending the Chronic Disease Clinic in Alwazarat Health center were randomly selected to participate in the study. Patients aged 18 years and above, who willingly agreed to participate, were included in the study. Self-reported questionnaire was used to determine patient level of enablement. Descriptive statistics such as mean and median were calculated and binary logistic regression was employed to determine the predictors of patient’s enablement to chronic disease. Results: Our results show that five hundred and sixty five (565) out of (604) patients participated in the study with 86.6% response rate. Type 2 diabetes mellitus affecting 40.65% while hypertension affecting 37.79% of the patients in Al wazarat health center. Patient’s enablement to chronic disease was very low and ranged between 2.41 and 1.53 out of 5.0. Binary logistic regression shows that age (male: OR;0.84, 95% CI, 0.72 - 1.04, female. OR;1.04, 95% CI 0.88 - 1.39), marital status (male: OR;0.72, 95% CI 0.54 - 1.11, female: OR 1.01;95% CI 0.82 - 1.29), patient educational level and number of problems discussed with physician and consultation length between male patients and their physician were statistically significant and correlated with patients enablement to chronic disease (P < 0.05). Conclusion: This study shows that patient’s enablement in chronic disease is very low but constitutes an important arm in patients care management. It should be considered as a measurable patient outcome from healthcare services. More prospective studies on this important topic are highly recommended.

ZTE Enables Calls from the World's Highest Point

ZTE Corporation, a leading global provider of telecommunications equipment and network solutions, announced that it has successfully deployed eight 3G stations in the Mt. Everest National Park including at Mt Everest base camp, 5,180 meters above sea level, to provide quality voice and 3G broadband services for local residents and mountaineers from all around the world preparing to climb the world＇s highest mountain.

Enablers, Difficulties and Features to Implement E-Learning Technology in Conventional Higher Education: Case Study and Prototype Implication

Supporting higher education with modern technologies like E-Learning is very important for one country to improve quality of education, to meet student’s expectations and to continue teaching-learning and training when face to face education is impossible. However, it is in its preliminary stage in developing countries like Ethiopia. This study examined the enabling factors, difficulties and opportunities of E-Learning implementation in Assosa University (ASU), Ethiopia. Its purpose is to find the enabling factors, difficulties and opportunities of E-Learning implementation in ASU and developing prototype of E-Learning system to show its practicality and to identify approaches of students’ and lecturers towards E-Learning. The study employed questionnaires, observation and interview to gather the required information. A sample of 309 students and 64 Lecturers randomly selected from 7 colleges and two schools as well as ICT workers and other concerned bodies in the university. Also prototyping as a methodology was used to implement and test the proposed system for proof of concept. This study investigating the possibility of implementing E-Learning in ASU and important enablers, difficulties and opportunities is identified. Also the E-Learning platform of the university is developed and introduced for students and lecturers to show its practicality. Most students and lecturers showed good motivation in E-Learning implementation and they assumed that it is useful for the university. The result shows that although there are difficulties to implement E-Learning in ASU, the possibility of fully implementing E-Learning in the University is relatively high with mixed method. With this, the approaches of students and lecturers are positively viewed and the opportunities are very noticeable in the University. So, conventional higher education can practically implement E-Learning with mixed approach to use as supportive tool for educational improvements and to reduce physical presence.

Widespread haploid-biased gene expression enables sperm-level natural selection

Sperm are haploid,but must be functionally equivalent to distribute alleles equally among progeny.Accordingly,gene products are shared through spermatid cytoplasmic bridges which erase phenotypic differences between individual haploid sperm.Here,we show that a large class of mammalian genes are not completely shared across these bridges.We term these genes"genoinformative markers"(GIMs)and show that a subset can act as selfish genetic elements that spread alleles unevenly through murine,bovine,and human populations.We identify evolutionary pressure to avoid conflict between sperm and somatic function as GIMs are enriched for testis-specific gene expression,paralogs,and isoforms.

基于嵌入式Web Enable的无线分布式测控系统

提出一种基于B/S构架的无线分布式测控的系统方案。该方案可以实现授权用户在Internet网络任何一点运用IE浏览器访问系统,并实现对下位机的测控。该系统已成功运用于"地下水资源无线监控系统"。

Co-opting signalling molecules enables logic-gated control of CAR T cells

Although chimeric antigen receptor(CAR)T cells have altered the treatment landscape for B cell malignancies,the risk of on-target,off-tumour toxicity has hampered their development for solid tumours because most target antigens are shared with normal cells1,2.Researchers have attempted to apply Boolean-logic gating to CAR T cells to prevent toxicity3-5;however,a truly safe and effective logic-gated CAR has remained elusive6.

An enzyme cascade enables production of therapeutic oligonucleotides in a single operation

Therapeutic oligonucleotides have emerged as a powerful drug modality with the potential to treat a wide range of diseases;however,the rising number of therapies poses a manufacturing challenge.Existing synthetic methods use stepwise extension of sequences immobilized on solid supports and are limited by their scalability and sustainability.We report a biocatalytic approach to efficiently produce oligonucleotides in a single operation where polymerases and endonucleases work in synergy to amplify complementary sequences embedded within catalytic self-priming templates.This approach uses unprotected building blocks and aqueous conditions.We demonstrate the versatility of this methodology through the synthesis of clinically relevant oligonucleotide sequences containing diverse modifications.

"Non-Traditional" Undertakings Enable Sichuan Farmers to Get Rich

We are publishing two stories about how in Sichuan Province, southwest China, farmers are getting rich with help from local governments by developing ＂non-traditional undertakings.＂ In China＇s current official terminology, a ＂non-traditional undertaking＂ can be anything other than the undertaking on which farmers, by tradition, depend for the bulk of their incomes-for example, crop and animal farming in most of China＇s farming areas. ＂Non-traditional＂ undertakings are more profitable than ＂traditional＂ ones.