上下颌轻中度拥挤下iTero Element 1口扫数字化模型的准确性

目的评估轻中度拥挤下采用iTero Element 1口内扫描仪获得的数字化模型和传统石膏模型测量数据的准确性。方法选取到昆明医科大学附属口腔医院前兴路门诊部就诊的37名患者,使用iTero Element 1口内扫描仪扫描获得数字化模型和藻酸盐取模获得同一患者传统石膏模型,比较数字化模型自动测量和传统石膏模型人工测量牙冠宽度、牙弓宽度、覆盖的差异。结果数字化模型和石膏模型的3次重复测量一致性检验ICC值均在0.9以上,均具有良好的可重复性。轻度拥挤组11、16、21、31、36、41、43、46牙冠宽度、14~24牙弓宽度、22覆盖,中度拥挤组11、23、16、31牙冠宽度、14~24牙弓宽度、11、12覆盖差异有统计学意义(P<0.05)。结论iTero数字化模型在轻中度拥挤时部分数据与手工测量石膏模型存在一定差异,可能是由于牙齿排列不齐,导致测量误差,但测量数据差异较小,不影响iTero数字化模型在临床中的应用。

转光棚膜对‘翠碧1号’烟苗生长及养分积累的影响

为探究转光膜在南平烟区烟草育苗中的应用效果,以‘翠碧1号’为试验材料,设普通聚乙烯棚膜和转光棚膜2个试验处理,研究转光膜对育苗棚内光照、温度和烟苗生长、养分积累的影响。结果表明,转光膜可调节育苗棚内光照和温度,转光膜处理育苗棚内13:00和14:00的光合有效辐射强度和透光率低于普通膜,而15:00和16:00的光合有效辐射强度和透光率高于普通膜。通过分析育苗棚内温度日变化发现,转光膜处理育苗棚内白天(8:00—16:00)的温度低于普通膜处理,其他时间高于普通膜处理。转光膜促进烟株的生长,与普通膜相比,转光膜处理的根系总根长、根表面积、根系体积、分支数等指标和根系干重、总干重显著高于普通膜处理。转光膜显著影响了养分的吸收和积累,转光膜处理烟苗地上部氮含量、地下部钙含量、整株磷含量和铜含量显著高于普通膜处理,而地上部钙含量和镁含量低于普通膜处理;转光膜处理促进了烟苗氮磷钾的累积。转光膜通过调节育苗棚内的光强和温度,促进烟苗生长和养分吸收,提高烟苗干物质和养分积累量。

Data Analysis of Chang'E-1 Gamma-Ray Spectrometer and Global Distribution of U,K,and Th Elemental Abundances

Gamma-ray spectrometer （GRS） is one of the main payloads on the Chang＇E-1 （CE-1） lunar probe, mainly aimed to detect the elemental abundances and distributions on the lunar surface. At 03：58 on 28 November 2007, it performed the first observation of the lunar gamma rays. As of 24 October 2008, 2105 h of effective gamma rays spectra had been acquired by CE-1 GRS, which covers the whole surface of the moon. This paper mainly describes the data processing procedures and methods of deriving the elemental abundances by using the CE-1 GRS time series corrected spectra： first, to bin data into pixels for mapping; then, to perform a background deduction of the cumulative spectra and obtain a peak area of the elements; and finally, to use the elemental abundances inversion model to produce the elemental abundances. Based on these processing methods, the global abundance maps of U, K, and Th at a 5°×5° equal-area pixel are acquired by CE-1 GRS data. The paper gives a preliminary analysis of the uncertainties of the elemental abundances.

Error estimates of H^1-Galerkin mixed finite element method for Schrdinger equation

An H^1-Galerkin mixed finite element method is discussed for a class of second order SchrSdinger equation. Optimal error estimates of semidiscrete schemes are derived for problems in one space dimension. At the same time, optimal error estimates are derived for fully discrete schemes. And it is showed that the H1-Galerkin mixed finite element approximations have the same rate of convergence as in the classical mixed finite element methods without requiring the LBB consistency condition.

Highly efficient H^1-Galerkin mixed finite element method (MFEM) for parabolic integro-differential equation

A highly efficient H1-Galerkin mixed finite element method （MFEM） is presented with linear triangular element for the parabolic integro-differential equation. Firstly, some new results about the integral estimation and asymptotic expansions are studied. Then, the superconvergence of order O（h^2） for both the original variable u in H1 （Ω） norm and the flux p = u in H（div, Ω） norm is derived through the interpolation post processing technique. Furthermore, with the help of the asymptotic expansions and a suitable auxiliary problem, the extrapolation solutions with accuracy O（h^3） are obtained for the above two variables. Finally, some numerical results are provided to confirm validity of the theoretical analysis and excellent performance of the proposed method.

C^1 natural element method for strain gradient linear elasticity and its application to microstructures

C^1 natural element method （C^1 NEM） is applied to strain gradient linear elasticity, and size effects on mi crostructures are analyzed. The shape functions in C^1 NEM are built upon the natural neighbor interpolation （NNI）, with interpolation realized to nodal function and nodal gradient values, so that the essential boundary conditions （EBCs） can be imposed directly in a Galerkin scheme for partial differential equations （PDEs）. In the present paper, C^1 NEM for strain gradient linear elasticity is constructed, and sev- eral typical examples which have analytical solutions are presented to illustrate the effectiveness of the constructed method. In its application to microstructures, the size effects of bending stiffness and stress concentration factor （SCF） are studied for microspeciem and microgripper, respectively. It is observed that the size effects become rather strong when the width of spring for microgripper, the radius of circular perforation and the long axis of elliptical perforation for microspeciem come close to the material characteristic length scales. For the U-shaped notch, the size effects decline obviously with increasing notch radius, and decline mildly with increasing length of notch.

P_1-nonconforming triangular finite element method for elliptic and parabolic interface problems

The lowest order Pl-nonconforming triangular finite element method （FEM） for elliptic and parabolic interface problems is investigated. Under some reasonable regularity assumptions on the exact solutions, the optimal order error estimates are obtained in the broken energy norm. Finally, some numerical results are provided to verify the theoretical analysis.

Long interspersed nuclear element ORF-1 protein promotes proliferation and resistance to chemotherapy in hepatocellular carcinoma

AIM:To clarify the specific roles and mechanisms of long interspersed nuclear element-1 ORF-1 protein [human long interspersed nuclear element-1(LINE-1),ORF-1p] in chemotherapeutic drug resistance and cell proliferation regulation in hepatocellular carcinoma(HCC) cells.METHODS:MTT assays were performed to identify the effect of the chemotherapeutic drug toxicity on HepG2 cells.Cell proliferation inhibition and the IC 50 were calculated by the Origin 8.0 software.Western blotting assays were performed to investigate whether LINE-1 ORF-1p modulates the expression of some important genes,including p53,p27,p15,Bcl-2,mdr,and p-gp.To corroborate the proliferation and anchor-independent growth results,the HepG2 cells were analyzed by flow cytometry to investigate the effect of LINE-1 ORF1p on the apoptosis regulation.RESULTS:LINE-1 ORF-1p contributed to the resistance to several chemotherapeutic drugs(cisplatin and epirubicin) in HepG2 cells.The IC 50 of the epirubicin and cisplatin increased from 36.04 nmol/L to 59.11 nmol/L or from 37.94 nmol/L to 119.32 nmol/L.Repression of LINE-1 ORF-1p expression by the siRNA could markedly enhance the response of HepG2 cells to the epirubicin and cisplatin.The IC 50 correspondingly decreased from 28.06 nmol/L to 3.83 nmol/L or from 32.04 nmol/L to 2.89 nmol/L.Interestingly,down-regulation of LINE-1 ORF-1p level by siRNA could promote the response of HepG2 cells to the paclitaxel.The IC 50 decreased from 35.90 nmol/L to 7.36 nmol/L.However,overexpression of LINE-1 ORF-1p did not modulate the paclitaxel toxicity in HepG2 cells.Further Western blotting revealed that LINE-1 ORF-1p enhanced mdr and p-gp gene expression.As a protein arrested in the nucleus,LINE-1 ORF-1p may function through modulating transcriptional activity of some important transcription factors.Indeed,LINE-1 ORF-1p promoted HepG2 cell proliferation,anchor-independent growth and protected the cells against apoptosis through modulating the expression of p15,p21,p53,and Bcl-2 genes.CONCLUSION:LINE-1 ORF-1p promotes HepG2 cell proliferation and plays an important role in the resistance of chemotherapeutic drugs.By establishing novel roles and defining the mechanisms of LINE-1 ORF1p in HCC chemotherapeutic drug resistance and cell proliferation regulation,this study indicates that LINE-1 ORF-1p is a potential target for overcoming HCC chemotherapeutic resistance.

miR-26b-3p靶向CREB1调控神经胶质瘤细胞的增殖、迁移及侵袭

目的探讨miR-26b-3p靶向调控环磷酸腺苷效应元件结合蛋白1(CREB1)表达水平影响胶质瘤细胞增殖、迁移和侵袭能力的分子机制。方法运用RT-qPCR和Western blotting检测不同级别胶质瘤中miR-26b-3p和CREB1的表达情况;生物信息学方法分析miR-26b-3p与CREB1结合的靶向序列。采用双荧光素酶报告基因检测miR-26b-3p对CREB1的靶向调控机制;将胶质瘤U251细胞分为对照组、miR-26b-3p mimic组及miR-26b-3p inhibitor组,采用Western blotting检测CREB1的表达变化,采用CCK-8法检测各组细胞增殖能力的影响,采用划痕实验检测各组细胞迁移能力的影响,采用Transwell检测各组细胞侵袭能力的影响,采用流式细胞术检测各组细胞凋亡的影响。结果miR-26b-3p的表达随着胶质瘤级别的增加而降低(P<0.05),而CREB1的表达则逐渐增加,差异有统计学意义(P<0.05);双荧光素酶报告基因结果显示miR-26b-3p可显著影响CREB13′UTR表达载体的荧光素酶活性,CREB1是miR-26b-3p下游靶基因。抑制miR-26b-3p表达可上调CERB1的表达,进而抑制细胞凋亡,促进胶质瘤细胞的增殖和侵袭。过表达miR-26b-3p可下调CERB1的表达,促进细胞凋亡,抑制胶质瘤细胞的增殖和侵袭(P<0.05)。结论miR-26b-3p可靶向调控CREB1的表达调节胶质瘤细胞的凋亡、增殖、迁移和侵袭,进而参与胶质瘤的发生发展。

Trace Element Levels, Cytokine Profile and Immune Activation Status in Plasma among Repeat Blood Donors with Asymptomatic HIV-1, HBV and HCV Infection

Imbalance of essential trace elements viz. Zinc, Selenium, Iron, Copper and Magnesium has been reported to influence disease course in HIV-1, HBV and HCV infections by altering immune status. A study was taken up to examine plasma levels of Th1 (IFN-γ and IL-2) and Th2 (IL-4 and IL-10) categories of cytokines and immune activation markers (TNF-α, TNFR I and TNFR II) in an asymptomatic group of HIV-1, HBV and HCV infected blood donors in relation to trace elements. Plasma levels of Zn, Se and Mg were depressed in all the three groups of blood donors (P < 0.001 for all). Levels of Cu and Fe were depressed in HIV-1 infection (P < 0.001 for both), but elevated in HBV and HCV infections (P < 0.015 and < 0.001 for Cu and < 0.001 for Fe in case of HBV and HCV infections respectively). IL-2 and IFN-γ were depressed in all the three groups of blood donors (P < 0.001). IL-4 and IL-10 levels were elevated in HBV and HCV infections (P < 0.001 for both). Immune activation markers were elevated in all the three groups of blood donors (P < 0.001 for all). HIV-1 infection showed positive correlations between Cu and IL-2, Zn and IFN-γ, and in HBV infection while positive correlations were found between Mg and TNFR I and TNFR II and Se with TNFR II. HCV infection showed a positive correlation between Se and IFN-γ (P < 0.001), Mg and IL-4 (P = 0.02), Fe and IL-10 (P < 0.01). The present study reveals possible relationship between trace element level alterations and alterations in cytokine and immune activation levels in HIV-1, HBV and HCV infection.

‘渝城1号’核桃矿质元素周年吸收规律研究

以‘渝城1号’核桃为研究对象,通过测定不同生育期的主要器官样品的生物量和N,P,K,Ca,Mg质量分数,总结分析核桃对5种矿质元素的吸收规律.结果表明:①开花坐果以后,核桃干物质快速增加,增加的干物质主要累积在果实、根和茎中,并在果实膨大期后,果实中的干物质累积量显著高于其他器官;②从各器官矿质元素质量分数上看,茎、营养枝和果枝中的N,P,K质量分数在展叶抽梢期最高,茎和营养枝中的Ca质量分数在果实膨大期最高,营养枝和果枝中Mg质量分数在落叶期最高.③从各器官矿质元素的累积量看,开花坐果前,N,P,K在根部的累积量最高.开花坐果后,果实中N,P,K的累积量逐渐升高.整个生育期,Ca在根部中的累积量最高,而Mg在果实膨大期前以根部累积量最高,之后在果实中的累积量最高;④5个矿质元素中,核桃对N,K,Ca吸收量大于P和Mg,其中核桃对N,P吸收量最大的时间在开花坐果期,对K和Mg吸收量最大的时间在坐果初期,对Ca吸收量最大的时间在果实膨大期.因此,开花坐果期到果实膨大期是核桃施肥的关键时期,在该时期应及时补充相应的矿质元素,以实现核桃的科学高效施肥.

A NOTE ON C^1-CURVED FINITE ELEMENT

This paper shows that the C1-curved finite element developed by Bernadon in general can not satisfy the essential boundary conditions on approximate boundary. Furthermore, a modified C1-curved finite element is given, which is compatible with the element of Argyris triangle and can satisfy the homogeneous Dirichlet boundary conditions onapproximate boundary.

Multiplex modification with rare earth elements and P for hypereutectic A1-Si alloys

The effect of rare earth （RE） elements on the morphologies and sizes of Si phases in the hypereutectic A1-Si alloys modified with P was investigated. The results show that the addition of La element to the hypereutectic A1-Si alloys can enhance the effect of P element on the modification of the primary Si phases. In the multiplex modification of RE-P, the primary Si phase is refiner and the shape of the eutectic Si is changed from long needle-like to short rod-like. Moreover, the agglomeration rate of the primary Si phase is slowed greatly. Even the melt is held for 6 h, the average size of the primary Si phase is still satisfied. The results analyzed by scanning electron microscope （SEM） indicate that La is richer at A1-Si interface than that in α-A1 or primary Si phase. The higher the La content in the A1-Si interface, the smaller the primary Si phase.

“1+X”证书制度下《建筑施工组织与管理》课程思政实践

对“1+X”证书制度下《建筑施工组织与管理》课程进行课程思政实践研究,分析课程思政现状,提出工程造价专业课程《建筑施工组织与管理》思政教学设计理念,将课程思政融入专业知识、专业技能及“1+X”(BIM)职业技能等级证书中。最后,给出该课程在思政设计、教学内容、教学方法、教学考核评价及“1+X”职业技能等级证书考核全过程与思政教育相融合的实践研究方案,从而实现人才培养中技能目标和思政目标的双向融通。

青岛市雾日PM_(1)中金属元素来源及健康风险评估

为了解青岛市雾日PM_(1)中金属元素的污染程度及其来源,并评估其对人类健康的危害,依据能见度及湿度数据对雾日进行划分,结合正定矩阵因子分解法(PMF)源解析模型和健康风险评价模型研究青岛市雾日亚微米颗粒物(PM_(1))中金属元素的来源和健康风险.结果表明,清洁雾日PM_(1)浓度略高于清洁日,而污染雾日PM_(1)浓度是霾日PM_(1)的1.11倍,清洁日的3.07倍.秋冬季雾日金属元素受人为源影响,K元素含量最高;夏季雾日的主要贡献元素是典型的地壳元素Ca、Fe、Al及海盐Na元素.PMF结果表明秋冬季雾日PM_(1)中金属元素主要来自煤/生物质燃烧、机动车源、地壳源、海盐源、船舶源和工业源;夏季雾日PM_(1)中金属元素主要来自煤/生物质燃烧、机动车源、地壳源、海盐源、船舶源和工业源.夏季采样点位临海,海雾频发,海盐源为夏季雾日金属元素的重要贡献.健康风险评估结果表明,成年人与儿童暴露于青岛秋冬季雾日PM_(1)的非致癌风险均低于阈值.成人和儿童呼吸途径Mn的非致癌风险最高,儿童手口摄食As的非致癌风险(HQAS=0.50)最高.As(3.1×10^(-6))和Cr(1.9×10^(-6))的致癌风险均已超过致癌风险阈值,建议加强含Mn、As、Cr排放源的管控.

秋冬季不同天气类型PM_(1)中金属元素污染来源及健康风险评估——以青岛市为例

于2018年~2022年秋冬季(每年11月至次年1月)开展大气PM_(1)逐日采样和20种金属元素分析,分析了不同天气类型下金属元素污染特征及来源,并评估了其健康风险.结果表明,沙尘日金属元素含量(2022.88±2298.00ng/m^(3))高于其他天气,霾日、污染雾日分别为清洁日的2.06倍和1.70倍.致癌金属元素(Ni、Cd、As、Cr、Co、Pb)和非致癌金属元素(Mn、Zn、Cu、V、Al、Ba)在霾日和污染雾日富集程度更高.正定矩阵因子模型结果表明,PM_(1)中金属元素主要来自机动车源、煤/生物质燃烧源、海盐源、地壳源、工业源和船舶源,其中雾日和霾日来自机动车和煤/生物质燃烧源的贡献达72.1%以上,清洁雾日船舶源贡献比上升至1.9%.后向气流轨迹分析表明霾日主要受西北向中长距离污染气团传输影响.污染雾日受海陆环流下本地排放和高污染气团长距离输送共同影响,沙尘日以西北向长、中距离沙尘气团输送为主导,同时受近距离沙尘气团二次回流影响.PM_(1)中金属元素经呼吸途径的非致癌风险可忽略,但As和Cr的终生致癌风险高于10^(-6)的阈值但低于10^(-4),且污染雾日和霾日风险概率最高,建议继续加强燃煤、冶金和电镀等工业过程As和Cr污染控制,重点防控秋冬季重污染事件中的污染雾日、霾日等天气.

Study on Element Contents in Tobacco Leaves from Different Positions of Tobacco CB-1

[Objectives]This study was conducted to clarify the differences and abundance or deficiency of various elements in tobacco leaves from different positions of CB-1 in Zhangping City,so as to guide the production of precision fertilization.[Methods]The element contents of CB-1 were determined by an X-ray fluorescence spectrometer.[Results]In terms of the contents of carbon,the upper leaves(45.73%)were significantly higher than the cutters(41.53%),and the lower leaves(43.15%)had no significant differences from both of them.For the content of oxygen element,there was no significant difference between the cutters(44.77%)and the lower leaves(43.40%),both of which were significantly higher than the upper leaves(40.75%).The contents of nitrogen,phosphorus,sulfur,boron and nickel all increased with the increase of the position,and they were significantly higher in the upper leaves than in the cutters and lower leaves.The contents of potassium,calcium,magnesium,chlorine and silicon all decreased with the increase of the position,and the lower leaves were significantly higher than the cutters and upper leaves.The contents of aluminum,titanium and chromium increased significantly with the increase of the position.The contents of strontium,zirconium and arsenic decreased significantly with the increase of the position.The element contents of tobacco leaves had significant or extremely significant differences between different parts of CB-1 in Zhangping City mostly.The contents of nitrogen,potassium,calcium,sulfur,chlorine,molybdenum and nickel in tobacco leaves were appropriate,and the contents of manganese and iron were too high,while the contents of phosphorus and copper were slightly low.The contents of magnesium,silicon,boron and sodium were too low.[Conclusions]This study can guide targeted fertilization and regulation of element contents in the tobacco leaves from different parts in production,and is of great significance to improving the production,quality and industrial availability of CB-1.

C1-Conforming Quadrilateral Spectral Element Method for Fourth-Order Equations

This paper is devoted to Professor Benyu Guo's open question on the C1-conforming quadrilateral spectral element method for fourth-order equations which has been endeavored for years. Starting with generalized Jacobi polynomials on the reference square, we construct the C1-conforming basis functions using the bilinear mapping from the reference square onto each quadrilateral element which fall into three categories-interior modes, edge modes, and vertex modes. In contrast to the triangular element, compulsively compensatory requirements on the global C1-continuity should be imposed for edge and vertex mode basis functions such that their normal derivatives on each common edge are reduced from rational functions to polynomials, which depend on only parameters of the common edge. It is amazing that the C1-conforming basis functions on each quadrilateral element contain polynomials in primitive variables, the completeness is then guaranteed and further confirmed by the numerical results on the Petrov-Galerkin spectral method for the non-homogeneous boundary value problem of fourth-order equations on an arbitrary quadrilateral. Finally, a C1-conforming quadrilateral spectral element method is proposed for the biharmonic eigenvalue problem, and numerical experiments demonstrate the effectiveness and efficiency of our spectral element method.

Nonconforming <i>H</i>¹-Galerkin Mixed Finite Element Method for Pseudo-Hyperbolic Equations

Based on H1-Galerkin mixed finite element method with nonconforming quasi-Wilson element, a numerical approximate scheme is established for pseudo-hyperbolic equations under arbitrary quadrilateral meshes. The corresponding optimal order error estimate is derived by the interpolation technique instead of the generalized elliptic projection which is necessary for classical error estimates of finite element analysis.

One‑Step Gas-Solid‑Phase Diffusion‑Induced Elemental Reaction for Bandgap‑Tunable Cu_(a)Agm_(1)Bim_(2)I_(n)/CuI Thin Film Solar Cells

Lead-free inorganic copper-silver-bismuth-halide materials have attracted more and more attention due to their environmental friendliness,high element abundance,and low cost.Here,we developed a strategy of one-step gas-solid-phase diffusioninduced reaction to fabricate a series of bandgap-tunable Cu_(a)Agm_(1)Bim_(2)I_(n)/CuI bilayer films due to the atomic diffusion effect for the first time.By designing and regulating the sputtered Cu/Ag/Bi metal film thickness,the bandgap of Cu_(a)Agm_(1)Bim_(2)I_(n)/CuI could be reduced from 2.06 to 1.78 eV.Solar cells with the structure of FTO/TiO_(2)/Cu_(a)Agm_(1)Bim_(2)I_(n)/CuI/carbon were constructed,yielding a champion power conversion efficiency of 2.76%,which is the highest reported for this class of materials owing to the bandgap reduction and the peculiar bilayer structure.The current work provides a practical path for developing the next generation of efficient,stable,and environmentally friendly photovoltaic materials.