Protoporphyirin-IX (PP-IX) is a precursor of the biosynthesis of the hemo group, most of the cytochromes and the chlorophylls. The PP-IX is used for medical purposes, and recently a report indicated that it exhibits a...Protoporphyirin-IX (PP-IX) is a precursor of the biosynthesis of the hemo group, most of the cytochromes and the chlorophylls. The PP-IX is used for medical purposes, and recently a report indicated that it exhibits a dual action since it can decrease or increase the genetic damage caused by N-nitroso-N-ethylurea (ENU) in somatic cells of Drosophila. PP-IX is known to be able to act as an anti-or pro-oxidant agent. The aim of the present research was to study the role of PP-IX on the lifespan of Drosophila melanogaster, taking into account the fact that increasing levels of ROS can accelerate the aging process. The Canton-S strain (CS) was used as well as Sod and Cat which are deficient in the endogenous enzymes, superoxide dismutase and catalase, respectively. Groups of females and males were treated separately with 5 mg/ml of PP-IX solution. The comparison of survival curves indicates that this pigment extended the lifespan of CS. In contrast, Sod strain showed that the opposite effect and had no effect in Cat strain. The fact that PP-IX reduces the mean lifespan in Sod deficient strain might suggest a pro-oxidant action of PP-IX, and consequently the cumulating of ROS as a superoxide could have a mutagenic effect as was shown recently. The results presented evidence of the dual effect of PP-IX.展开更多
Objective: The purpose of this study is to investigate whether the LE CAT, PROMIS PF CAT, Depression CAT, or Pain CAT can be used as a proxy for the EQ-5D-5L. Background: Patient-reported out-come measures have become...Objective: The purpose of this study is to investigate whether the LE CAT, PROMIS PF CAT, Depression CAT, or Pain CAT can be used as a proxy for the EQ-5D-5L. Background: Patient-reported out-come measures have become vital tools for physicians to understand the effectiveness and value of treatment and care. Methods: This study was conducted in 2012 with 116 patients that took the EQ-5D-5L and a number of patient-reported outcome instruments in a university orthopaedic clinic. Regression analyses were conducted to predict EQ-5D-5L index scores from the LE CAT, PROMIS PF CAT, Depression CAT, and Pain CAT. Results: All predictors, separately or combined, significantly predicted the EQ-5D-5L index scores (p < 0.0001). The LE CAT was the best predictor;it alone accounted for 37% of the variability in the EQ-5D-5L. When combining patient-reported outcome measures, the best predicting model was the one consisting of the LE CAT, Depression CAT and Pain CAT;they explained for 43.9% of the variance in EQ-5D-5L. Conclusions: The findings provide encouraging news that the LE CAT, PF CAT, Depression CAT and Pain CAT can be used alone or in combination as a proxy for the EQ-5D-5L. Researchers have the options of using these patient-reported outcome measures for economic evaluations and medical intervention studies.展开更多
文摘Protoporphyirin-IX (PP-IX) is a precursor of the biosynthesis of the hemo group, most of the cytochromes and the chlorophylls. The PP-IX is used for medical purposes, and recently a report indicated that it exhibits a dual action since it can decrease or increase the genetic damage caused by N-nitroso-N-ethylurea (ENU) in somatic cells of Drosophila. PP-IX is known to be able to act as an anti-or pro-oxidant agent. The aim of the present research was to study the role of PP-IX on the lifespan of Drosophila melanogaster, taking into account the fact that increasing levels of ROS can accelerate the aging process. The Canton-S strain (CS) was used as well as Sod and Cat which are deficient in the endogenous enzymes, superoxide dismutase and catalase, respectively. Groups of females and males were treated separately with 5 mg/ml of PP-IX solution. The comparison of survival curves indicates that this pigment extended the lifespan of CS. In contrast, Sod strain showed that the opposite effect and had no effect in Cat strain. The fact that PP-IX reduces the mean lifespan in Sod deficient strain might suggest a pro-oxidant action of PP-IX, and consequently the cumulating of ROS as a superoxide could have a mutagenic effect as was shown recently. The results presented evidence of the dual effect of PP-IX.
文摘Objective: The purpose of this study is to investigate whether the LE CAT, PROMIS PF CAT, Depression CAT, or Pain CAT can be used as a proxy for the EQ-5D-5L. Background: Patient-reported out-come measures have become vital tools for physicians to understand the effectiveness and value of treatment and care. Methods: This study was conducted in 2012 with 116 patients that took the EQ-5D-5L and a number of patient-reported outcome instruments in a university orthopaedic clinic. Regression analyses were conducted to predict EQ-5D-5L index scores from the LE CAT, PROMIS PF CAT, Depression CAT, and Pain CAT. Results: All predictors, separately or combined, significantly predicted the EQ-5D-5L index scores (p < 0.0001). The LE CAT was the best predictor;it alone accounted for 37% of the variability in the EQ-5D-5L. When combining patient-reported outcome measures, the best predicting model was the one consisting of the LE CAT, Depression CAT and Pain CAT;they explained for 43.9% of the variance in EQ-5D-5L. Conclusions: The findings provide encouraging news that the LE CAT, PF CAT, Depression CAT and Pain CAT can be used alone or in combination as a proxy for the EQ-5D-5L. Researchers have the options of using these patient-reported outcome measures for economic evaluations and medical intervention studies.
