Watermelon fruit undergoes distinct development stages with dramatic changes during fruit ripening.To date,the molecular mechanics of watermelon ripening remain unclear.Genetic and transcriptome evidences suggested th...Watermelon fruit undergoes distinct development stages with dramatic changes during fruit ripening.To date,the molecular mechanics of watermelon ripening remain unclear.Genetic and transcriptome evidences suggested that the ethylene response factor(ERF)gene ClERF069 may be an important candidate factor affecting watermelon fruit ripening.To dissect the roles of ClERF069 in fruit ripening,structure and phylogenetic analysis were performed using the amplified full-length sequence.Normal-ripening watermelon 97103,non-ripening watermelon PI296341-FR and the RIL population were used to analyze ClERF069 expression dynamics and the correlation with fruit ripening indexs.The results indicated that ClERF069 belongs to ERF family group VI and show high homology(83%identity)to melon ERF069-like protein.ClERF069 expression in watermelon flesh was negatively correlated with fruit lycopene content and sugar content during fruit ripening progress.Further transgenic evidences indicated that overexpression of 35S:ClERF069 in tomato noticeably delayed the ripening process up to 5.2 days.Lycopene,β-carotenoid accumulation patterns were altered and ethylene production patterns in transgenic fruits was significantly delayed during fruit ripening.Taken together,watermelon ethylene response factor ClERF069 was concluded to be a negative regulator of fruit ripening.展开更多
Members of the ERF Family of Transcription Factors play an important role in plant development and gene expression that regulates responses to biotic and abiotic stress.This work identified 36 ERF family genes in Coff...Members of the ERF Family of Transcription Factors play an important role in plant development and gene expression that regulates responses to biotic and abiotic stress.This work identified 36 ERF family genes in Coffea arabica within the AP2/ERF full domain,using the EST-based genomic resource of the Brazilian Coffee Genome Project.The ERF family genes were classified into nine of the ten existing groups through phylogenetic analysis of the deduced amino acid sequences and comparison with the sequences of the ERF family genes in Arabidopsis.In addition to the AP2 domain,other conserved domains were identified,typical of members of each group.The in silico analysis and expression profiling showed high levels of expression for libraries derived from tissues of fruits,leaves and flowers as well as for libraries subjected to water stress.These results suggest the participation of the ERF family genes of C.arabica in distinct biological functions,such as control of development,maturation,and responses to water stress.The results of this work imply in the selection of promising genes for further functional characterizations that will provide a better understanding of the complex regulatory networks related to plant development and responses to stress,opening up opportunities for coffee breeding programs.展开更多
AP2/ERF转录因子家族是植物中广泛存在的一类转录因子,AP2/ERF这类转录因子主要参与植物的细胞周期、生长发育以及生物和非生物胁迫相关基因的表达调控。由于拟南芥和油菜同属于芸薹属,具有相似的基因信息,利用油菜UniGene数据库,以拟南...AP2/ERF转录因子家族是植物中广泛存在的一类转录因子,AP2/ERF这类转录因子主要参与植物的细胞周期、生长发育以及生物和非生物胁迫相关基因的表达调控。由于拟南芥和油菜同属于芸薹属,具有相似的基因信息,利用油菜UniGene数据库,以拟南芥ERF转录因子保守序列为探针,通过电子克隆从一个UniGene C luster中分离得到三个同类的AP2/ERF转录因子,从cDNA序列、氨基酸序列的相似性、组成成分、理化性质、疏水性/亲水性分析、序列比对、进化树、功能域、二级结构、三级结构、无序化特性进行了预测和较为全面的分析。结果显示油菜来源的BnaERF1、BnaERF2和BnaERF3属于AP2/ERF转录因子的B-2亚族,是亲水性蛋白,在蛋白质的三级结构上与AtERF1相似。蛋白质无序化分析发现,油菜BnaERF1、BnaERF2和BnaERF3无序化程度小于拟南芥AtERF1。设计引物通过PCR和RT-PCR方法分别从甘蓝型双低油菜沪油15幼苗的DNA和cDNA中扩增了上述基因,初步分析BnaERF2没有内含子,BnaERF1和BnaERF3有内含子。另外,通过EST丰度分析显示,该类转录因子的表达最高峰在种子中,其次为花,在芽、茎和分生组织中没有检测出表达的存在。展开更多
基金This work was financially supported by the National Key R&D Program of China(Grant No.2018YFD0100703)the Beijing Municipal Science and Technology Project(Grant No.D171100007617001)+4 种基金the Beijing Academy of Agricultural and Forestry Sciences(Grant Nos.QNJJ201733,KJCX20200202)the Ministry of Agriculture and Rural Affairs of China(Grant No.CARS-25)the Beijing Scholar Program(Grant No.BSP026)Beijing Innovation Consortium of Agriculture Research System(Grant No.BAIC10-2020)the Bagui Scholar Program(Grant No.2016A11).
文摘Watermelon fruit undergoes distinct development stages with dramatic changes during fruit ripening.To date,the molecular mechanics of watermelon ripening remain unclear.Genetic and transcriptome evidences suggested that the ethylene response factor(ERF)gene ClERF069 may be an important candidate factor affecting watermelon fruit ripening.To dissect the roles of ClERF069 in fruit ripening,structure and phylogenetic analysis were performed using the amplified full-length sequence.Normal-ripening watermelon 97103,non-ripening watermelon PI296341-FR and the RIL population were used to analyze ClERF069 expression dynamics and the correlation with fruit ripening indexs.The results indicated that ClERF069 belongs to ERF family group VI and show high homology(83%identity)to melon ERF069-like protein.ClERF069 expression in watermelon flesh was negatively correlated with fruit lycopene content and sugar content during fruit ripening progress.Further transgenic evidences indicated that overexpression of 35S:ClERF069 in tomato noticeably delayed the ripening process up to 5.2 days.Lycopene,β-carotenoid accumulation patterns were altered and ethylene production patterns in transgenic fruits was significantly delayed during fruit ripening.Taken together,watermelon ethylene response factor ClERF069 was concluded to be a negative regulator of fruit ripening.
文摘Members of the ERF Family of Transcription Factors play an important role in plant development and gene expression that regulates responses to biotic and abiotic stress.This work identified 36 ERF family genes in Coffea arabica within the AP2/ERF full domain,using the EST-based genomic resource of the Brazilian Coffee Genome Project.The ERF family genes were classified into nine of the ten existing groups through phylogenetic analysis of the deduced amino acid sequences and comparison with the sequences of the ERF family genes in Arabidopsis.In addition to the AP2 domain,other conserved domains were identified,typical of members of each group.The in silico analysis and expression profiling showed high levels of expression for libraries derived from tissues of fruits,leaves and flowers as well as for libraries subjected to water stress.These results suggest the participation of the ERF family genes of C.arabica in distinct biological functions,such as control of development,maturation,and responses to water stress.The results of this work imply in the selection of promising genes for further functional characterizations that will provide a better understanding of the complex regulatory networks related to plant development and responses to stress,opening up opportunities for coffee breeding programs.
文摘AP2/ERF转录因子家族是植物中广泛存在的一类转录因子,AP2/ERF这类转录因子主要参与植物的细胞周期、生长发育以及生物和非生物胁迫相关基因的表达调控。由于拟南芥和油菜同属于芸薹属,具有相似的基因信息,利用油菜UniGene数据库,以拟南芥ERF转录因子保守序列为探针,通过电子克隆从一个UniGene C luster中分离得到三个同类的AP2/ERF转录因子,从cDNA序列、氨基酸序列的相似性、组成成分、理化性质、疏水性/亲水性分析、序列比对、进化树、功能域、二级结构、三级结构、无序化特性进行了预测和较为全面的分析。结果显示油菜来源的BnaERF1、BnaERF2和BnaERF3属于AP2/ERF转录因子的B-2亚族,是亲水性蛋白,在蛋白质的三级结构上与AtERF1相似。蛋白质无序化分析发现,油菜BnaERF1、BnaERF2和BnaERF3无序化程度小于拟南芥AtERF1。设计引物通过PCR和RT-PCR方法分别从甘蓝型双低油菜沪油15幼苗的DNA和cDNA中扩增了上述基因,初步分析BnaERF2没有内含子,BnaERF1和BnaERF3有内含子。另外,通过EST丰度分析显示,该类转录因子的表达最高峰在种子中,其次为花,在芽、茎和分生组织中没有检测出表达的存在。
