期刊文献+
共找到89篇文章
< 1 2 5 >
每页显示 20 50 100
Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit Ripening 被引量:11
1
作者 Ming Zhou Shaogui Guo +6 位作者 Shouwei Tian Jie Zhang Yi Ren Guoyi Gong Changbao Li Haiying Zhang Yong Xu 《Horticultural Plant Journal》 SCIE 2020年第4期247-256,共10页
Watermelon fruit undergoes distinct development stages with dramatic changes during fruit ripening.To date,the molecular mechanics of watermelon ripening remain unclear.Genetic and transcriptome evidences suggested th... Watermelon fruit undergoes distinct development stages with dramatic changes during fruit ripening.To date,the molecular mechanics of watermelon ripening remain unclear.Genetic and transcriptome evidences suggested that the ethylene response factor(ERF)gene ClERF069 may be an important candidate factor affecting watermelon fruit ripening.To dissect the roles of ClERF069 in fruit ripening,structure and phylogenetic analysis were performed using the amplified full-length sequence.Normal-ripening watermelon 97103,non-ripening watermelon PI296341-FR and the RIL population were used to analyze ClERF069 expression dynamics and the correlation with fruit ripening indexs.The results indicated that ClERF069 belongs to ERF family group VI and show high homology(83%identity)to melon ERF069-like protein.ClERF069 expression in watermelon flesh was negatively correlated with fruit lycopene content and sugar content during fruit ripening progress.Further transgenic evidences indicated that overexpression of 35S:ClERF069 in tomato noticeably delayed the ripening process up to 5.2 days.Lycopene,β-carotenoid accumulation patterns were altered and ethylene production patterns in transgenic fruits was significantly delayed during fruit ripening.Taken together,watermelon ethylene response factor ClERF069 was concluded to be a negative regulator of fruit ripening. 展开更多
关键词 WATERMELON ethylene response factor erf transgenic tomato RIPENING
下载PDF
Ethylene response factor BnERF2-like (ERF2.4) from Brassica napus L.enhances submergence tolerance and alleviates oxidative damage caused by submergence in Arabidopsis thaliana 被引量:2
2
作者 Yanyan Lv Sanxiong Fu +2 位作者 Song Chen Wei Zhang Cunkou Qi 《The Crop Journal》 SCIE CAS CSCD 2016年第3期199-211,共13页
Ethylene response factor proteins play an important role in regulating a variety of stress responses in plants,but their exact functions in submergence stress are not well understood.In this study,we isolated BnE RF2.... Ethylene response factor proteins play an important role in regulating a variety of stress responses in plants,but their exact functions in submergence stress are not well understood.In this study,we isolated BnE RF2.4 from Brassica napus L.to study its function in submergence tolerance.The expression of the BnE RF2.4 gene in B.napus and the expression of antioxidant enzyme genes in transgenic Arabidopsis were analyzed by quantitative RT-PCR.The expression of BnE RF2.4 was induced by submergence in B.napus and the overexpression of BnE RF2.4 in Arabidopsis increased the level of tolerance to submergence and oxidative stress.A histochemical method detected lower levels of H_2O_2,O^(·-)_2and malondialdehyde(MDA) in transgenic Arabidopsis.Compared to the wild type,transgenic lines also had higher soluble sugar content and higher activity of antioxidant enzymes,which helped to protect plants against the oxidative damage caused by submergence.It was concluded that BnE RF2.4 increased the tolerance of plants to submergence stress and may be involved in regulating soluble sugar content and the antioxidant system in defense against submergence stress. 展开更多
关键词 ethylene response factor SUBMERGENCE OXIDATIVE damage ECTOPIC expression Arabidopsis Antioxidant ENZYME
下载PDF
小麦ERF亚族转录因子参与逆境胁迫的研究进展
3
作者 崔德周 王丽丽 +5 位作者 陈祥龙 李永波 黄琛 隋新霞 楚秀生 樊庆琦 《山东农业科学》 北大核心 2024年第2期176-180,共5页
小麦是中国三大粮食作物之一,其生长发育过程中会受到多种逆境胁迫的影响。AP2/EREBP是植物特有的一个庞大的转录因子超家族,普遍参与生长发育和逆境胁迫应答等生物学进程。ERF类转录因子是AP2/EREBP转录因子超家族的一个亚族。本研究... 小麦是中国三大粮食作物之一,其生长发育过程中会受到多种逆境胁迫的影响。AP2/EREBP是植物特有的一个庞大的转录因子超家族,普遍参与生长发育和逆境胁迫应答等生物学进程。ERF类转录因子是AP2/EREBP转录因子超家族的一个亚族。本研究结合国内外相关研究进展,简要综述了小麦ERF亚族转录因子的结构特征与分布,重点阐述近年来小麦ERF亚族转录因子响应高盐、干旱、低温、重金属、病原菌侵染等逆境胁迫的功能和机制研究进展,最后展望了ERF亚族转录因子的研究方向和应用前景。 展开更多
关键词 小麦 erf亚族 转录因子 胁迫响应 研究进展
下载PDF
乙烯响应因子(ERFs)在植物花青素合成中的调控作用 被引量:3
4
作者 刘菊 张会灵 +2 位作者 张中华 赵亚男 张菊平 《植物遗传资源学报》 CAS CSCD 北大核心 2023年第3期615-623,共9页
花青素是一种天然色素,可以作为清除自由基的重要天然抗氧化剂,其富含的多种化合物在医疗保健方面十分重要。花青素影响果蔬成熟、口感、色泽,对植物的非生物和生物胁迫产生保护作用,因此优化花青素含量被视为许多园艺作物的育种目标。... 花青素是一种天然色素,可以作为清除自由基的重要天然抗氧化剂,其富含的多种化合物在医疗保健方面十分重要。花青素影响果蔬成熟、口感、色泽,对植物的非生物和生物胁迫产生保护作用,因此优化花青素含量被视为许多园艺作物的育种目标。本研究阐述了乙烯响应因子(ERFs)作为乙烯信号传递的次级转录因子响应植物激素信号并能产生反馈调节,以多种方式介导了乙烯调控植物花青素生物合成的过程。在作用方式上,ERFs主要通过与转录因子互作、激活转录因子、与MBW形成调控复合物或直接激活结构基因启动子的方式调控植物花青素的生物合成。本研究旨在为后续深入阐明ERF调控不同物种花青素生物合成的机制、探究果蔬成熟后期花青素快速积累与乙烯释放量增加之间存在的联系提供理论依据。 展开更多
关键词 erf 乙烯 花青素 转录因子
下载PDF
Identification and characterization of MeERF genes and their targets in pathogen response by cassava(Manihot esculenta) 被引量:2
5
作者 Yuhui Hong Yong Xiao +8 位作者 Na Song Shousong Zhu Rui Zhao Ke Li Mengting Geng Xiaohui Yu Honggang Wang Wei Xia Yinhua Chen 《The Crop Journal》 SCIE CSCD 2021年第5期1145-1153,共9页
Cassava,Manihot esculenta Crantz (Me),is a major dietary source of calories for over 700 million people in tropical regions.The production of cassava is constantly threatened by cassava bacterial blight (CBB),caused b... Cassava,Manihot esculenta Crantz (Me),is a major dietary source of calories for over 700 million people in tropical regions.The production of cassava is constantly threatened by cassava bacterial blight (CBB),caused by Xanthomonas axonopodis pv.manihotis (Xam).The gene resources for CBB-resistant breeding of cassava are limited.In model plant species,ethylene response factors play important roles in response to pathogen infection.In this study,cassava ethylene response factors (MeERFs) were identified and characterized as the first step in studying their potential for CBB-resistant breeding of cassava.In the cassava genome 155 MeERFs were identified,of which 23 were induced by Xam infection.The promoter regions of204 genes harbored GCC-box that had the potential to interact with MeERFs.Using 37 transcriptomes derived from Xam infection treatment,four gene co-expression modules for the MeERFs and GCC-box containing genes were constructed.Six MeERFs were associated with two GCC-box containing genes:transcription initiation factor TFIIE subunit beta (MeTFIIE),and histone-lysine N-methyltransferase ASHR1 (MeASHR1).Dual-luciferase reporter assays showed that MeERF10 and MeERF58 positively regulated Me TFIIE;MeERF137 negatively regulated Me TFIIE;MeERF10 and MeERF137 positively regulated Me ASHR1;and MeERF35 negatively regulated Me ASHR1.The four MeERFs may mediate pathogen response by regulating the expression of the two GCC-box containing genes. 展开更多
关键词 CASSAVA ethylene response factor Expression profile Co-expression analysis PATHOGEN
下载PDF
GsERF6基因过表达对水稻耐盐碱性的影响 被引量:3
6
作者 才晓溪 胡冰霜 +5 位作者 沈阳 王研 陈悦 孙明哲 贾博为 孙晓丽 《作物学报》 CAS CSCD 北大核心 2023年第2期561-569,共9页
乙烯响应因子(ERF)是植物特有的一类转录因子,在响应非生物胁迫中具有重要作用。本研究通过生物信息学发现,野生大豆耐盐碱ERF转录因子GsERF6与水稻ERF同源蛋白的氨基酸序列相似性很高,均包含1个高度保守的AP2结构域。为探究GsERF6基因... 乙烯响应因子(ERF)是植物特有的一类转录因子,在响应非生物胁迫中具有重要作用。本研究通过生物信息学发现,野生大豆耐盐碱ERF转录因子GsERF6与水稻ERF同源蛋白的氨基酸序列相似性很高,均包含1个高度保守的AP2结构域。为探究GsERF6基因在水稻耐盐碱应答中的作用,通过遗传转化、PCR和半定量RT-PCR鉴定,获得了2个纯合的GsERF6过表达转基因水稻株系。表型鉴定表明,200 mmol L^(-1) NaHCO3处理下GsERF6转基因水稻的存活率、相对含水量、超氧化物歧化酶、过氧化物酶、过氧化氢酶活性、可溶性糖和脯氨酸含量均显著高于对照,活性氧积累则反之。实时荧光定量PCR分析表明,40 mmol L^(-1) NaHCO3处理6 h后渗透调节基因OsP5CS2和OsLEA14在GsERF6转基因水稻中的表达量显著高于对照。本研究表明,水稻中GsERF6的过表达可通过提高ROS清除水平、渗透调节能力及胁迫应答基因的表达来提高其耐盐碱性。 展开更多
关键词 水稻 耐盐碱 乙烯响应因子 野生大豆 Gserf6
下载PDF
Identification,Structure Analyses and Expression Pattern of the ERF Transcription Factor Family in Coffea arabica
7
作者 Silvia Graciele Hülse de Souza Tiago B.dos Santos +5 位作者 Douglas S.Domingues Anne Bernadac Mondher Bouzayen Luiz F.P.Pereira Giuliano Degrassi Valéria Carpentieri-Pípolo 《Journal of Botanical Research》 2021年第1期32-45,共14页
Members of the ERF Family of Transcription Factors play an important role in plant development and gene expression that regulates responses to biotic and abiotic stress.This work identified 36 ERF family genes in Coff... Members of the ERF Family of Transcription Factors play an important role in plant development and gene expression that regulates responses to biotic and abiotic stress.This work identified 36 ERF family genes in Coffea arabica within the AP2/ERF full domain,using the EST-based genomic resource of the Brazilian Coffee Genome Project.The ERF family genes were classified into nine of the ten existing groups through phylogenetic analysis of the deduced amino acid sequences and comparison with the sequences of the ERF family genes in Arabidopsis.In addition to the AP2 domain,other conserved domains were identified,typical of members of each group.The in silico analysis and expression profiling showed high levels of expression for libraries derived from tissues of fruits,leaves and flowers as well as for libraries subjected to water stress.These results suggest the participation of the ERF family genes of C.arabica in distinct biological functions,such as control of development,maturation,and responses to water stress.The results of this work imply in the selection of promising genes for further functional characterizations that will provide a better understanding of the complex regulatory networks related to plant development and responses to stress,opening up opportunities for coffee breeding programs. 展开更多
关键词 AP2/erf COFFEE ethylene Transcription factor
下载PDF
芒果乙烯信号转导关键基因MiCTR1的克隆与序列分析
8
作者 袁芳 王春艳 +4 位作者 李丽 许丹妮 李志红 甘婷 隆宇涵 《热带作物学报》 CSCD 北大核心 2024年第10期2035-2043,共9页
芒果属于典型的呼吸跃变型果实,采后代谢旺盛且对乙烯非常敏感,CTR1是乙烯信号转导途径的负调控因子,在乙烯信号通路中发挥着核心作用。为了研究芒果CTR1在芒果采后贮藏过程中的表达模式及可能的作用,本研究从台农1号芒果转录组数据库... 芒果属于典型的呼吸跃变型果实,采后代谢旺盛且对乙烯非常敏感,CTR1是乙烯信号转导途径的负调控因子,在乙烯信号通路中发挥着核心作用。为了研究芒果CTR1在芒果采后贮藏过程中的表达模式及可能的作用,本研究从台农1号芒果转录组数据库中筛选1个CTR基因(MiCTR1),利用生物学方法对其编码的蛋白质进行测序分析,并对MiCTR1基因在芒果后熟过程中经乙烯抑制剂1-MCP处理后的表达模式进行分析。结果表明:MiCTR1的开放阅读框(ORF)长度为1551 bp,编码516个氨基酸,预测蛋白的分子式为C_(2503)H_(3932)N_(712)O_(797)S_(26),总原子数为7970,分子量为57.58 kDa,理论等电点(pI)为5.72,负电荷和正电荷的氨基酸残基总数分别为64和50个,脂肪系数为70.78,亲水性总平均值为–0.596,有111个磷酸化位点,且以丝氨酸(Ser)的磷酸化修饰为主,苏氨酸(Thr)为辅,无跨膜结构,无信号肽,亚细胞预测分析显示其定位于细胞核。蛋白质结构域预测发现,MiCTR1蛋白含有1个保守的PB1结构域,位于氨基酸序列189~285处。系统进化分析表明,MiCTR1与扁桃(Prunus dulcis)、桃(Prunus persica)、甜樱桃(Prunus avium)、杏(Prunus armeniaca)、梅(Prunus mume)的亲缘关系较近。荧光定量PCR结果显示,MiCTR1的相对表达量在芒果后熟过程中升高,且经1-MCP处理后其表达量下调。本研究结果为芒果后熟的分子机制提供基础。 展开更多
关键词 芒果 乙烯响应转录因子 CTR 克隆 表达分析
下载PDF
Cotton Major Latex Protein 28 Functions as a Positive Regulator of the Ethylene Responsive Factor 6 in Defense against Verticillium dahliae 被引量:13
9
作者 Chun-Lin Yang 《Molecular Plant》 SCIE CAS CSCD 2015年第3期399-411,共13页
In this study, we identified a defense-related major latex protein (MLP) from upland cotton (designated GhMLP28) and investigated its functional mechanism. GhMLP28 transcripts were ubiquitously present in cotton p... In this study, we identified a defense-related major latex protein (MLP) from upland cotton (designated GhMLP28) and investigated its functional mechanism. GhMLP28 transcripts were ubiquitously present in cotton plants, with higher accumulation in the root. Expression of the GhMLP28 gene was induced by Verticillium dahliae inoculation and was responsive to defense signaling molecules, including ethylene, jas- monic acid, and salicylic acid. Knockdown of GhMLP28 expression by virus-induced gene silencing re- sulted in increased susceptibility of cotton plants to V. dahliae infection, while ectopic overexpression of GhMLP28 in tobacco improved the disease tolerance of the transgenic plants. Further analysis revealed that GhMLP28 interacted with cotton ethylene response factor 6 (GhERF6) and facilitated the binding of GhERF6 to GCC-box element. Transient expression assay demonstrated that GhMLP28 enhanced the tran- scription factor activity of GhERF6, which led to the augmented expression of some GCC-box genes. GhMLP28 proteins were located in both the nucleus and cytoplasm and their nuclear distribution was dependent on the presence of GhERF6. Collectively, these results demonstrate that GhMLP28 acts as a positive regulator of GhERF6, and synergetic actions of the two proteins may contribute substantially to protection against V. dahliae infection in cotton plants. 展开更多
关键词 major latex protein ethylene responsive factor interaction disease tolerance COTTON
原文传递
Ethylene Response Factor TERF1 Enhances Glucose Sensitivity in Tobacco through Activating the Expression of Sugar-related Genes 被引量:4
10
作者 Ang Li Zhijin Zhang +1 位作者 Xue-Chen Wang Rongfeng Huang 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2009年第2期184-193,共10页
Ethylene response factor (ERF) proteins are important plant-specific transcription factors. Increasing evidence shows that ERF proteins regulate plant pathogen resistance, abiotic stress response and plant developme... Ethylene response factor (ERF) proteins are important plant-specific transcription factors. Increasing evidence shows that ERF proteins regulate plant pathogen resistance, abiotic stress response and plant development through interaction with different stress responsive pathways. Previously, we revealed that overexpression of TERF1 in tobacco activates a cluster gene expression through interacting with GCC box and dehydration responsive element (DRE), resulting in enhanced sensitivity to abscisic acid (ABA) and tolerance to drought, and dark green leaves of mature plants, indicating that TERF1 participates in the integration of ethylene and osmotic responses. Here we further report that overexpression of TERF1 confers sugar response in tobacco. Analysis of the novel isolated tomato TERF1 promoter provides information indicating that there are many cis-acting elements, including sugar responsive elements (SURE) and W box, suggesting that TERF1 might be sugar inducible. This prediction is confirmed by results of reverse transcription-polymerase chain reaction amplification, indicating that transcripts of TERF1 are accumulated in tomato seedlings after application of glucose. Further investigation indicates that the expression of TERF1 in tobacco enhances sensitivity to glucose during seed germination, root and seedling development, showing a decrease of the fresh weight and root elongation under glucose treatment. Detailed investigations provide evidence that TERF1 interacts with the sugar responsive cis-acting element SURE and activates the expression of sugar response genes, establishing the transcriptional regulation of TERF1 in sugar response. Therefore, our results deepen our understanding of the glucose response mediated by the ERF protein TERF1 in tobacco. 展开更多
关键词 ethylene response factor protein Terf1 GLUCOSE sugar responsive gene TOBACCO
原文传递
New Role for an Old Rule: N-end Rule-Mediated Degradation of Ethylene Responsive Factor Proteins Governs Low Oxygen Response in Plants 被引量:1
11
作者 Francesco Licausi Chiara Pucciariello Pierdomenico Perata 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2013年第1期31-39,共9页
The N-end rule pathway regulates protein degradation, which depends on exposed N-terminal sequences in prokaryotes and eukaryotes. In plants, conserved and specific enzymes stimulate selective proteolysis. Although a ... The N-end rule pathway regulates protein degradation, which depends on exposed N-terminal sequences in prokaryotes and eukaryotes. In plants, conserved and specific enzymes stimulate selective proteolysis. Although a number of developmental and growth phenotypes have been reported for mutants in the N-end rule, its function has remained unrelated to specific physiological pathways. The first report of the direct involvement of the N-end rule in stress responses focused on hypoxic signaling and how the oxygen-dependent oxidation of cystein promotes the N-end rule-mediated degradation of ethylene responsive factor (ERF)-VII proteins, the master regulators of anaerobic responses. It has been suggested that plants have evolved specific mechanisms to tune ERF-VII availability in the nucleus. In this review, we speculate that ERF-VII proteins are reversibly protected from degradation via membrane sequestration. The oxidative response in plants subjected to anoxic conditions suggests that reactive oxygen and nitrogen species (reactive oxygen species and reactive nitrogen species) may interact or interfere with the N-end rule pathway-mediated response to hypoxia. 展开更多
关键词 ethylene response factor FLOODING HYPOXIA N-end rule pathway ubiquitin.
原文传递
小麦ERF转录因子W17互作蛋白的筛选和解析 被引量:9
12
作者 邱志刚 徐兆师 +3 位作者 郑天慧 李连城 陈明 马有志 《作物学报》 CAS CSCD 北大核心 2011年第5期803-810,共8页
来自小麦的ERF转录因子W17基因参与胁迫应答,过表达W17可显著提高转基因拟南芥的抗旱性和抗病性。本研究构建了小麦cDNA文库,通过酵母双杂技术筛选W17的互作蛋白,进一步解析ERF蛋白的作用机制。将pGBKT7-W17质粒、pGADT7和小麦文库混合... 来自小麦的ERF转录因子W17基因参与胁迫应答,过表达W17可显著提高转基因拟南芥的抗旱性和抗病性。本研究构建了小麦cDNA文库,通过酵母双杂技术筛选W17的互作蛋白,进一步解析ERF蛋白的作用机制。将pGBKT7-W17质粒、pGADT7和小麦文库混合转入酵母细胞AH109,在SD/-Trp/-Leu/-His/-Ade营养缺陷型平板上培养,挑选直径大于2mm的克隆,在SD/Raf/Gal/X-gal平板上划线培养,筛选蓝色克隆。将筛出的克隆测序、BLAST分析,得到4类与W17相互作用的候选蛋白,分别是胁迫相关功能蛋白、翻译后修饰蛋白、1,5-二磷酸核酮糖羧化酶/加氧酶(Rubisco)大亚基/小亚基以及功能未知蛋白。互作验证表明,HSP90和PPR蛋白与W17有相互作用关系。这些候选蛋白参与信号转导或免疫过程,暗示W17在植物的逆境信号转导、下游基因转录调控,甚至在翻译过程都有重要作用。 展开更多
关键词 酵母双杂交系统 erf 蛋白互作 信号转导 小麦
下载PDF
ERFs转录因子及其在植物胁迫应答中的作用 被引量:8
13
作者 卢丞文 潘晓琪 朱本忠 《生物技术通报》 CAS CSCD 北大核心 2012年第3期22-27,共6页
ERFs(ethylene-responsive factors)是植物特有的一类转录因子,位于乙烯信号转导途径的下游,具有一个高度保守的含58或59个氨基酸的DNA结合域,通过结合相关基因的启动子顺式作用元件调控植物生物和非生物胁迫反应。综述ERF转录因子的结... ERFs(ethylene-responsive factors)是植物特有的一类转录因子,位于乙烯信号转导途径的下游,具有一个高度保守的含58或59个氨基酸的DNA结合域,通过结合相关基因的启动子顺式作用元件调控植物生物和非生物胁迫反应。综述ERF转录因子的结构特征和分类及其在植物抗逆作用中的研究进展。 展开更多
关键词 erfs 转录因子 乙烯 胁迫应答
下载PDF
病原诱导的小麦转录因子TaERF1b基因的分离和表达 被引量:7
14
作者 董娜 张增艳 辛志勇 《中国农业科学》 CAS CSCD 北大核心 2008年第4期946-953,共8页
【目的】研究一个病原诱导的小麦ERF转录因子基因在对纹枯病菌、赤霉病菌防御反应中的作用。【方法】应用生物信息学、RT-PCR、RACE方法,从赤霉病菌诱导的小麦中,分离ERF转录因子基因的全长cDNA序列;利用半定量RT-PCR方法,分析该基因对... 【目的】研究一个病原诱导的小麦ERF转录因子基因在对纹枯病菌、赤霉病菌防御反应中的作用。【方法】应用生物信息学、RT-PCR、RACE方法,从赤霉病菌诱导的小麦中,分离ERF转录因子基因的全长cDNA序列;利用半定量RT-PCR方法,分析该基因对小麦纹枯病菌、赤霉病菌及MeJA、ET和SA处理的应答表达情况。【结果】从赤霉病菌诱导的抗赤霉病小麦品种苏麦3号cDNA中,分离出1个编码ERF转录因子基因的全长cDNA序列。该基因暂被命名为TaERF1b,编码由280个氨基酸组成的蛋白质TaERF1b。TaERF1b具有保守的ERF/AP2结构域,但TaERF1b蛋白全长氨基酸序列与已克隆的ERF蛋白同源性较低(<36.5%)。TaERF1b基因表达分析的结果表明,纹枯病菌、赤霉病菌侵染可快速诱导抗病小麦中TaERF1b基因的上调表达,该基因转录表达水平在防卫相关激素乙烯、茉莉酸处理早期显著增强,而且TaERF1b对外源乙烯、茉莉酸处理的响应时期早于对纹枯病菌、赤霉病菌的响应时期。【结论】分离出一个受病原诱导的小麦ERF新基因TaERF1b,其编码蛋白TaERF1b为植物ERF转录因子家族的一个新成员,可能通过茉莉酸、乙烯信号途径介导小麦对纹枯病菌、赤霉病菌的防御反应。 展开更多
关键词 小麦 erf转录因子 克隆 纹枯病菌 赤霉病菌 表达 防御反应
下载PDF
ERF转录因子在植物抗逆境胁迫的研究进展 被引量:13
15
作者 刘建光 王永强 +3 位作者 张寒霜 赵俊丽 郭娴 孟宪鹏 《华北农学报》 CSCD 北大核心 2013年第S1期214-218,共5页
ERF(Ethylene responsive factors)转录因子广泛存在于各类植物中,通过识别和结合不同的顺式元件参与植物逆境胁迫的应答。主要介绍ERF转录因子结构特征及其功能特性,在植物应答生物和非生物的胁迫中可能的调控机制,并讨论了今后的研究... ERF(Ethylene responsive factors)转录因子广泛存在于各类植物中,通过识别和结合不同的顺式元件参与植物逆境胁迫的应答。主要介绍ERF转录因子结构特征及其功能特性,在植物应答生物和非生物的胁迫中可能的调控机制,并讨论了今后的研究重点。 展开更多
关键词 erf转录因子 转录调控 胁迫应答 信号转导途径
下载PDF
病原诱导的中间偃麦草ERF转录因子基因的克隆及其表达特性 被引量:3
16
作者 姚乌兰 张增艳 +1 位作者 陈亮 辛志勇 《作物学报》 CAS CSCD 北大核心 2007年第9期1405-1410,共6页
应用RT-PCR、RACE技术,从病原诱导的中间偃麦草叶片cDNA中,分离出1个编码ERF基因的全长cDNA序列,该基因暂命名为TiERF1 a,编码由292个氨基酸组成的蛋白质,具有ERF转录因子典型的结构,即保守的AP2/ERF DNA结合域、核定位位点和酸性激活区... 应用RT-PCR、RACE技术,从病原诱导的中间偃麦草叶片cDNA中,分离出1个编码ERF基因的全长cDNA序列,该基因暂命名为TiERF1 a,编码由292个氨基酸组成的蛋白质,具有ERF转录因子典型的结构,即保守的AP2/ERF DNA结合域、核定位位点和酸性激活区。TiERF1a的氨基酸序列与一个水稻ERF蛋白OsBIERF3具有66%的一致性,与拟南芥AtERF1(O80337)一致性仅39.7%,为植物ERF转录因子家族B3亚群的一个新成员。表达分析结果表明,纹枯病菌、赤霉病菌侵染可诱导TiERF1 a基因的上调表达,与防卫相关的激素乙烯、茉莉酸也可诱导该基因上调表达,且TiERF1 a对外源乙烯、茉莉酸的响应时期早于对纹枯病菌、赤霉病菌响应时期,说明TiERF1 a可能通过乙烯、茉莉酸信号途径参与寄主调控对纹枯病菌、赤霉病菌的防御反应。 展开更多
关键词 中间偃麦草 erf转录因子 克隆 表达
下载PDF
植物ERFs类转录因子在逆境胁迫中的作用 被引量:5
17
作者 李聪 郭天麒 +2 位作者 梁小红 王英博 韩烈保 《生物技术通报》 CAS CSCD 北大核心 2011年第4期1-6,共6页
Ethylene responsive factors(ERFs)是植物中特有的一类重要的转录因子。ERFs转录因子含有一段高度保守的DNA结合区域,被称之为ERF区域。ERFs类转录因子可以直接与GCC-box等启动子结合,从而转录激活功能基因的表达,调节植株的抗性应答... Ethylene responsive factors(ERFs)是植物中特有的一类重要的转录因子。ERFs转录因子含有一段高度保守的DNA结合区域,被称之为ERF区域。ERFs类转录因子可以直接与GCC-box等启动子结合,从而转录激活功能基因的表达,调节植株的抗性应答。它们参与植物的生长发育、代谢、生物胁迫和非生物胁迫相关的应答过程,并且在水杨酸和脱落酸和茉莉酸信号转导途径中发挥一定的作用,调控植物多个信号转导途径。现针对ERFs类转录因子在植物逆境胁迫中的研究进展进行讨论。 展开更多
关键词 植物 erf转录因子 结构 逆境应答
下载PDF
病原诱导的小麦ERF转录因子TaERF1b的原核表达及纯化 被引量:6
18
作者 董娜 张增艳 辛志勇 《植物遗传资源学报》 CAS CSCD 2008年第3期283-287,共5页
为了得到纯化的TaERF1b活性蛋白,将TaERF1 b基因含有AP2/ERF结构域的片段插入原核表达载体pGEX-4T-1的多克隆位点中,构建GST-TaERF1b融合蛋白表达载体,并转化到大肠杆菌BL21(DE3)中。0.1mmol/L IPTG即能诱导融合蛋白表达,37℃诱导4h或3... 为了得到纯化的TaERF1b活性蛋白,将TaERF1 b基因含有AP2/ERF结构域的片段插入原核表达载体pGEX-4T-1的多克隆位点中,构建GST-TaERF1b融合蛋白表达载体,并转化到大肠杆菌BL21(DE3)中。0.1mmol/L IPTG即能诱导融合蛋白表达,37℃诱导4h或30℃诱导8h,融合蛋白均以包涵体的形式表达,16℃诱导12h,融合蛋白不表达。包涵体经溶解及稀释复性后,过GST亲和层析柱,获得纯化的融合蛋白,考马斯亮蓝法测得纯化蛋白的浓度约为0.5μg/μl,凝胶阻滞实验表明包涵体复性成功,所得蛋白具有生物活性。 展开更多
关键词 erf(ethylene-responsive factor) Taerflb 原核蛋白表达 包涵体
下载PDF
ERF转录因子及其在烟草抗逆性改良中的应用 被引量:12
19
作者 李文正 张海文 +1 位作者 王俊英 黄荣峰 《生物技术通报》 CAS CSCD 2006年第4期30-34,共5页
转录因子调控的基因表达是植物逆境应答反应的关键环节。乙烯反应因子(ERF:ethylene responsive factor)在植物抗逆反应中发挥重要的调控作用,并成为近年来植物分子生物学领域的研究前沿。本文综述目前ERF及其对烟草抗逆遗传改良的相关... 转录因子调控的基因表达是植物逆境应答反应的关键环节。乙烯反应因子(ERF:ethylene responsive factor)在植物抗逆反应中发挥重要的调控作用,并成为近年来植物分子生物学领域的研究前沿。本文综述目前ERF及其对烟草抗逆遗传改良的相关研究进展。 展开更多
关键词 乙烯应答因子 烟草 抗逆性 遗传改良
下载PDF
杂交杨转录因子Pdt-ERF3抗锈病基因表达 被引量:7
20
作者 王峰 程诗评 +4 位作者 牛春阳 李丹蕾 陈俏丽 张瑞芝 沈圣智 《森林工程》 2016年第6期10-14,共5页
落叶松-杨栅锈菌(Melampsora larici-populina)严重危害多种杨树。为探究转录调控因子ERF3在杂交杨抗锈菌反应中的分子机理,克隆到弱感病性树种杂交杨(Populus deltoides×P.trichocarpa)和感病树种欧美杨(Populus×euramerican... 落叶松-杨栅锈菌(Melampsora larici-populina)严重危害多种杨树。为探究转录调控因子ERF3在杂交杨抗锈菌反应中的分子机理,克隆到弱感病性树种杂交杨(Populus deltoides×P.trichocarpa)和感病树种欧美杨(Populus×euramericana)ERF3基因:Pdt-ERF3和Pnd-ERF3。采用荧光定量Q-PCR技术对接种E4强致病性落叶松-杨栅锈菌前后PdtERF3和Pnd-ERF3基因表达量变化进行分析。落叶松-杨栅锈菌接种后,Pdt-ERF3表达量在12 hpi达到高峰期,PndERF3表达量在24 hpi显著上调,在96 hpi达到高峰期。Pnd-ERF3比Pdt-ERF3表达量高峰期推迟84 h。除168 hpi时Pnd-ERF3与Pdt-ERF3表达均有下降外,2个基因表达量在锈菌侵染的多个阶段均存在差异。根据拟南芥(Arabidopsis thaliana)同源基因预测Pdt-ERF3受丝裂原活化蛋白激酶MAPK6调控。本研究为进一步系统研究杨树ERF基因与抗性反应的调控机理及杨树抗病育种工作提供理论依据。 展开更多
关键词 转录调控因子 erf 乙烯 落叶松-杨栅锈菌
下载PDF
上一页 1 2 5 下一页 到第
使用帮助 返回顶部