Cytotoxic effect of methanolic extracts of Fritillaria imperialis bulbs and Eryngium caucasicum leaves on hepatoma and colon cancer cells

Objective: To evaluate antitumor activities of Fritillaria imperialis and Eryngium caucasicum methanolic extracts on human hepatoma (HepG2) and colon cancer (HCT116) cell lines in comparison to human foreskin fibroblasts as the normal cells. Methods: Methanolic extracts of Fritillaria imperialis and Eryngium caucasicum were prepared by the maceration method. The effect of the extracts at various concentrations (100, 200, 400, 600, and 800 μg/mL) on cell survival was evaluated using the MTT method. Besides, fluorescence staining was used to evaluate death patterns of the cells. Results: MTT assay showed that Fritillaria imperialis significantly decreased the viability of all cell lines after 24 and 48 hours of treatments. However, Eryngium caucasicum extract did not show any significant cytotoxicity effect on the cell lines. Fluorescence staining revealed that Fritillaria imperialis induced apoptosis of HCT116 cells at 550 μg/mL. Conclusions: Fritillaria imperialis extract has antiproliferative and cytotoxic effects on HCT116 and HepG2 cancer cells and therefore, may serve as an anticancer agent.

Ethnobotanical and traditional uses, phytochemical constituents and biological activities of Eryngium species growing in Iran

Objective: Eryngium with the 274 accepted species, is the largest genus of Apiaceae family which are distributed all over the world and have been used in traditional remedies to manage various ailments in different nations. Ten species of Eryngium have been identified in Iran including E. caeruleum M.B.(syn: E. caucasicum Trautv.), E. creticum Lam., E. bungei Boiss., E. billardieri F. Delaroche.(syn: E. kotschyi Boiss.), E. glomeratum Lam.(syn: E. parviflorum Sm.), E. bornumulleri Nab., E. pyramidale Boiss.& Husson., E. noeanum Boiss., E. wanaturi Woron.(syn: E. woronowii Bordz.), and E. thyrsoideum Boiss. The aim of the present research is to review pharmacological activity, and phytochemical constituents as well as ethnobotany and traditional uses of Iranian species of Eryngium. Materials and methods: Electronic databases including PubMed, Scopus, Science Direct (ISI Web of Knowledge) and Embase library were comprehensively searched for research on Eryngium. The search period was from 1966 to October 2018. The related articles were selected according to the inclusion and exclusion criterias in our study. Results: A total of 57 papers were enrolled in analyses. The findings showed that Iranian species of Eryngium, had a noticeable diverse of traditional medicinal uses and also broad range of pharmacological activities as well as various phytochemical compounds. Some remarkable biological and pharmacological activities of these species have been demonstrated in present scientific studies, including antimicrobial, cytotoxic and anticancer, anti-inflammatory, analgesic and antinociceptive activities as well as antioxidant, antidiabetic, anti-snake and anti-scorpion venom effects. Conclusion: Iranian Eryngium species have enormous potential for prospective preparation of herbal medicinal products and are good candidates for discovering new drugs.

滨海刺芹愈伤组织提取物中4种多酚含量测定及抗氧化、抗炎活性研究

采用热水回流法制备滨海刺芹愈伤组织提取物,基于高效液相色谱法建立同时测定滨海刺芹愈伤组织提取物中迷迭香酸、隐绿原酸、金丝桃苷、槲皮素-3-O-(6’’-O-乙酰基)-β-D-吡喃葡萄糖苷四种多酚含量的方法,测定提取物对DPPH自由基、ABTS自由基清除能力。同时通过CCK-8法检测筛选样品给药浓度,建立痤疮丙酸杆菌诱导HaCaT细胞炎症模型,评价其抗炎功效。结果表明,提取物中隐绿原酸含量最高,为5.23 mg/g;迷迭香酸、槲皮素-3-O-(6’’-O-乙酰基)-β-D-吡喃葡萄糖苷、金丝桃苷的含量依次为1.30,0.50,0.08 mg/g。提取物具有较好的抗氧化、抗炎能力,对DPPH自由基、ABTS自由基清除能力的IC_(50)值分别为0.3239,0.2965 mg/mL,对痤疮丙酸杆菌诱导的HaCaT细胞分泌IL-1β,IL-8也具有显著抑制作用(P<0.05)。

Phenolic composition and antioxidant properties of Eryngium maritimum (sea holly)

Objective: To examine the antioxidant potential of Tunisian Eryngium maritimum (E. maritimum) leaf, root and stems extracts, as well as their phenolic compositions. Methods: The antioxidant activity of different extracts was assessed using DPPH free radical and hydrogen peroxide scavenging assays. Phenolic profiles were determined by means of liquid chromatography (HPLC-DAD). Results: All plant parts were a rich source of phenolics. Polyphenols and flavonoids were present in leaf extracts. E. maritimum leaf extracts displayed the strongest H2O2 scavenging activity (IC50 = 76.83 μg/mL) and the highest DPPH scavenging activity value (IC50 = 47.87 μg/mL) compared to other extracts. Good relationships were observed between antioxidant activities and the total phenolic and flavonoid contents. Nine bioactive compounds were detected in E. maritimum extracts. Conclusions: Our results provided evidence that E. maritimum could be an interesting source of natural antioxidant that can be used to treat divers diseases.

刺芫荽总黄酮提取工艺优化及其抑菌活性研究

目的:刺芫荽总黄酮提取工艺优化并研究抑菌作用。方法:采用超声提取方法提取刺芫荽中的总黄酮,基于单因素实验分析并利用正交实验来优化刺芫荽中的总黄酮提取工艺,通过滤纸片法和肉汤稀释法实验测出最小抑菌浓度(MIC),进而研究刺芫荽黄酮类成分对大肠杆菌、金黄色葡萄球菌的抑制能力。结果:最佳提取工艺为超声温度70℃、超声时间40 min、乙醇体积分数80%、料液比1∶25(g∶mL),此条件下总黄酮提取率为1.480%。刺芫荽总黄酮对金黄色葡萄球菌的抑制作用大于大肠杆菌,最小抑菌浓度分别为0.250 mg/mL和0.500 mg/mL。结论:优化后的超声提取工艺方法可行性强,稳定性好,操作简单,为刺芫荽总黄酮的进一步开发奠定了基础。

滨海刺芹的化学成分及其功效研究进展

滨海刺芹(Eryngium maritimum),别名海冬青,属于伞形科刺芹属。由于生长环境特殊,其所含的次生代谢产物具有独特的结构以及功能。对滨海刺芹的多种活性成分进行了总结,并对其美白、抗衰、祛痘等多种功效作用进行了综述,对其在化妆品领域中的应用具有一定意义。

中国伞形科变豆菜亚科的果实解剖特征及其系统学意义

对中国伞形科变豆菜亚科 (Saniculoideae) 2属 (变豆菜属SaniculaL .和刺芹属EryngiumL .) 13种植物的果实形态结构进行了比较解剖学观察 ,从果实横切面形状及其合生面宽度、外果皮和中果皮外突所呈皮刺和鳞片或瘤状体的形态及皮刺中木化厚壁细胞组织的分布、外果皮细胞形状及其角质层厚度、中果皮细胞层数和结晶体类型及分布、色素块沉积、伴生分泌管和油管的大小及分布等方面对上述各属种进行了观察和分析 ,归纳出各属的果实解剖特征 ,探讨了属间演化水平 ,认为刺芹属果实较变豆菜属果实演化程度高。结合已有的研究结果 ,讨论了该亚科与伞形科天胡荽亚科 (Hydrocotyloideae)间的区别及其演化关系。

海南野芫荽挥发油成分分析

目的分析海南野芫荽全草的挥发油成分。方法以海南野芫荽为原料,以水蒸气蒸馏法提取挥发油,采用气相色谱-质谱联用技术对挥发油成分进行分析,用面积归一法来测定各成分的相对百分含量。结果从海南野芫荽挥发油中共鉴定出38种化合物,占挥发油总量的100%,其中主要成分为十二烯醛(22.18%)、2,4,5三甲基苯甲醛(14.27%)、1,11-十二二烯(11.15%)、2-十二碳烯酸(8.95%)、十二烷酸(7.78%)、环癸烷(7.32%)、N-棕榈酸(5.15%)等。结论通过对海南野芫荽挥发油成分的分析与鉴定,可看出海南产野芫荽与云南产野芫荽挥发油成分明显不同,所得实验结果为合理开发利用海南野芫荽提供了科学依据。

刺芹总黄酮的微波辅助提取及抑菌性研究

以云南刺芹为原料,微波辅助提取其中的总黄酮,并对刺芹总黄酮粗提浓缩物对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌3种细菌进行抑菌性测试。在单因素试验的基础上,采用正交试验优化了刺芹总黄酮的提取工艺。最佳工艺条件为料液比1∶25 g/m L,乙醇体积分数70%,微波功率210 W,提取时间60 s,总黄酮平均得率为5.07%。刺芹总黄酮粗提液对金黄色葡萄球菌、枯草芽孢杆菌的抑菌效果较强,对大肠杆菌无抑制效果。

洋芫荽对胸膜炎模型大鼠炎性细胞因子的影响

选用大鼠胸膜炎模型，观察洋芫荽对实验动物炎性细胞因子如肿瘤坏死因子（ＴＮＦ－α）、白细胞介素－６（ＩＬ－６）、一氧化氮（ＮＯ） 的影响，结果表明：洋芫荽能降低 ＴＮＦ－α、ＩＬ－６、及ＮＯ的含量水平，提示其治疗感染性疾病的作用可能与之有关。

刺芫荽水提液中总酚酸含量的测定

目的：测定刺芫荽水提液中总酚酸（TPA）的含量。方法：总酚酸的含量测定采用可见分光光度法,以没食子酸为对照品,以0.6%Fe Cl3~0.9%K3[Fe（CN）6]（1∶0.9）混合液为显色剂,在720 nm波长处进行测定。结果：分光光度法测定总酚酸在所试浓度范围内（0.224~1.120μg/m L）线性关系良好,r=0.9996。总酚酸的平均回收率为97.3%,RSD为1.7%。结论：本法简单快捷,重现性好,可作为刺芫荽总酚酸的质控方法。

刺芫荽挥发性成分研究

以野生刺芫荽为原料,采用同时蒸馏萃取法提取其挥发性成分,应用气相色谱―质谱法对提取物进行化学成分分析.实验结果表明,野生刺芫荽提取物中共鉴定出24种化合物,占总峰面积的65.94%,质量分数达到1.00%以上的有6种物质,其挥发性成分中醛酮类化合物相对质量分数达到58.05%.

3种药食植物雾培生长效果研究

采用雾培的方法,对奇亚、土人参和刺芫荽3种药食植物进行生长效果研究,结果表明,雾培的奇亚株高和根长分别是土培的1.6和3.8倍,雾培的土人参和刺芫荽植株平均鲜重分别是土培的2.9和3.4倍,说明雾培能明显促进3种药食植物的生物量积累。

洋芫荽挥发油成分分析

采用气质联用仪对洋芫荽的挥发性成分进行了系统分析。初步鉴定出16种化合物,并弄清洋芫荽的主要挥发性成分。

刺芫荽中总黄酮含量的测定

目的用紫外分光光度法测定刺芫婪中总黄酮的含量。方法用60％的乙醇提取总黄酮，以芦丁为对照品，在紫外510nm处测定。结果芦丁检测浓度在0．0128—0．064mg／L范围内与吸光度呈现良好的线性关系，R^2=0．9996，加样平均回收率101．69％，RSD=0．57％（n=6）；用乙醇回流提取法提取的总黄酮含量可达到1．079％。结论本方法简便、快速、准确，操作简单易行，适用于黄酮类物质含量的测定。

水蒸气蒸馏法提取广东刺芫荽挥发油的成分分析

目的:分析广东刺芫荽植物的挥发油成分。方法:2020年3月在广州医科大学附属第六医院通过水蒸气蒸馏法提取广东刺芫荽的挥发油成分,采用气相色谱–质谱联用技术对挥发油成分进行分析,并比较其他地区刺芫荽挥发油成分的差异。结果:从广东刺芫荽挥发油中共鉴定出23种化学物,其中含量大于5 %的成分有反式–2–十二烯醛(35.82 %)、2–十二烯醛(24.53 %)、反式–2–十四醛(7.42 %)。结论:提取广东刺芫荽的挥发油成分分析,与云南、海南刺芫荽挥发油成分明显有区别,为今后进一步开发研究广东刺芫荽提供依据。

刺芫荽中多糖的提取工艺优化及含量测定

对刺芫荽中多糖的提取工艺进行优化并测定其含量。采用水提法提取了刺芫荽多糖,以葡萄糖作为对照品,用苯酚硫酸法测定多糖含量;对刺芫荽多糖的提取进行料液比、提取时间、提取温度、提取次数单因素考察,并根据其结果以正交设计法优化提取工艺。优选出最佳提取工艺:料液比1∶20,提取时间150 min,提取温度80℃,提取次数2次。优化后的提取工艺方法可行,操作简单,为刺芫荽多糖的进一步开发奠定了基础。

正交试验法优选刺芫荽总黄酮提取工艺的研究

为了优选刺芫荽总黄酮的提取工艺。以总黄酮提取量为指标,考察提取温度、乙醇浓度、回流时间、料液比等单因素对刺芫荽总黄酮提取率的影响,通过正交试验筛选出最佳提取工艺。结果表明,影响刺芫荽总黄酮提取率的因素依次为:提取温度(A)>乙醇浓度(D)>提取时间(B)>料液比(C);筛选出刺芫荽中总黄酮的最佳条件为A_3B_3C_1D_1,即提取温度90℃、料液比为1∶30,提取时间1.5 h,乙醇浓度50%。在此条件下刺芫荽总黄酮的提取率可达到1.879%。该提取工艺合理,方法简单,可为刺芫荽进一步的研究开发提供依据。

刺芫荽种子萌发特性初步研究

为探究刺芫荽种子萌发的适宜条件,进行了室温贮藏时间、温度、播种基质含水量及播种深度对剌芫荽种子萌发影响的测定。结果表明:刺芫荽种子在室温下贮藏时,随着贮藏时间的延长,其活力逐渐下降,在生产实践中,种子在室温下的贮藏时间不宜超过12个月;刺芫荽种子萌发的适宜温度为30℃左右,适宜的播种深度为土壤表层以下不超过1.5cm,种子萌发出苗要求较高的基质含水量,适宜的基质含水量在60%左右。

刺芫荽总黄酮超声提取工艺及抗氧化活性研究

本研究目的是对刺芫荽中总黄酮超声辅助提取的工艺条件进行优化,并对刺芫荽总黄酮的抗氧化活性进行评价。以总黄酮得率为指标,采用单因素试验,分别对超声波提取时乙醇浓度、料液比、提取温度和提取时间的适宜水平进行筛选;在此基础上,采用正交试验设计对总黄酮超声提取条件进行优化,结果显示,刺芫荽总黄酮超声提取的最佳工艺条件为乙醇浓度50%、料液比1∶25(g/mL)、水浴温度80℃、超声时间50 min,在此条件下总黄酮得率为1.385%。并通过测定总黄酮提取物对羟基自由基(·OH)和DPPH自由基的清除率以及对大豆油和玉米油的抗油脂过氧化活性,评价了其体外的抗氧化活性,结果显示,刺芫荽总黄酮对·OH和DPPH自由基均具有较好的清除作用,并具有较好的抗油脂过氧化活性。利用正交试验方法得到的刺芫荽总黄酮超声辅助提取最佳工艺条件提取到的总黄酮具有较好的抗氧化活性。