Extract of the eschar tissue taken from the rat which had been given full-thickness burn of20% TBSA was found to be inhibitory to the respiratory function of the mitochondria and theenergization of the inner membrane....Extract of the eschar tissue taken from the rat which had been given full-thickness burn of20% TBSA was found to be inhibitory to the respiratory function of the mitochondria and theenergization of the inner membrane. The inhibition was also demonstrated by the 1: 2 and 1: 4and 1: 8 dilutions of the eschar extract. The extract of normal skin only showed weak inhibitory cf-fect. The 1: 4 dilution of normal skin had no (?) on mitochondrial functions. There is a widegap between the malonaldehyde (MDA) contents of these two kinds of extracts. An apparent posi-tive correlation was found between MDA content and degree of inhibition. However, on close ex-amination, it was found that the MDA content is not the direct cause of the decrease of therespiratory control ratio (RCR). The increase of MDA content is probably related to the destruc-tion of the lipid bilayer of the mitochondria. Data from the energization experiment show that theenergization process is compromised when the destruction of the membrane has proceeded to a cer-tain degrce. The inhibition of mitochondrial respiratory function becomes evident when encrgizationfunction is still normal. Therefore at least two mechanisms are operating in the inhibition process.Further work is needed to elucidate the problem.展开更多
Mediterranean spotted fever(MSF)has been diagnosed clinically in the Crimean Peninsula since the 1930s.We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms...Mediterranean spotted fever(MSF)has been diagnosed clinically in the Crimean Peninsula since the 1930s.We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever,maculopapular rash,and eschar.Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 Rickettsia protein genes.The strain causing clinical illness was characterized as Rickettsia conorii subspecies conorii Malish 7.This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management.展开更多
When febrile illnesses develop a rash in children, parents are very concerned about a serious disease. Many rashes associated with fever are caused by infectious diseases. Rashes are generally nonspecific and play a s...When febrile illnesses develop a rash in children, parents are very concerned about a serious disease. Many rashes associated with fever are caused by infectious diseases. Rashes are generally nonspecific and play a supportive role in differential diagnosis, but for some diseases, the appearance of the rash is essential in making a diagnosis. Here is presented the case of a 4-year-old boy with high fever, headache, abdominal pain, vomiting, diarrhea, and a generalized maculo-papular rash including palms and soles. On physical examination were found a black eschar, cervical lymphadenopathy, and hepatosplenomegaly. Laboratory findings resulted in moderate leukocytosis and moderate involvement of the liver and renal function. Based on this finding, a diagnosis of Mediterranean Spotted Fever was performed. The child recovered after medication with azithromycin. Because there is no reliable test that can confirm MSF in its early stages, the diagnosis is commonly made on the basis of clinical findings, so a high index of suspicion should be maintained while evaluating a child with fever and rash.展开更多
Objective:To study epidemiology,clinical manifestations,and laboratory parameters of scrub typhus in Hadoti region of Rajasthan,India.Methods:A cross-sectional study was conducted on 50 patients with scrub typhus betw...Objective:To study epidemiology,clinical manifestations,and laboratory parameters of scrub typhus in Hadoti region of Rajasthan,India.Methods:A cross-sectional study was conducted on 50 patients with scrub typhus between August 2022 and November 2022.Scrub typhus was diagnosed by ELISA IgM scrub typhus antibody.The data about demography,vital parameters,and lab investigations were collected and analyzed.Results:Ninety-two percent of the patients were from a rural background(92%),86% were farmers and the majority were females.Most patients were 40-60 years old.The major complaints were fever(100%),myalgia(100%),headache(82%),and body swelling(80%).Major complications were pneumonia(68%),multiple organ dysfunction syndrome(62%),hepatitis(44%),and acute kidney injury(42%).Triglyceride levels were>200 mg/dL in 80% of patients,albumin<3.5 g/dL in 98% and eschar mark was present in 24%of patients.Conclusions:Scrub typhus is a serious acute febrile illness that can lead to multi-organ dysfunction and is associated with significant mortality.Increasing awareness regarding disease in endemic regions,early screening of patients,and treatment as early as possible could help prevent the patient from severe life-threatening complications.展开更多
目的探讨右美托咪定对大面积烧伤患者静脉全身麻醉（全麻）切痂植皮术应激反应的影响。方法选择择期行大面积烧伤削痂植皮术患者60例,ASAⅠ、Ⅱ级,随机均分成生理盐水对照组（S组）和右美托咪定组（D组）。诱导前10 min D组静脉泵注负...目的探讨右美托咪定对大面积烧伤患者静脉全身麻醉（全麻）切痂植皮术应激反应的影响。方法选择择期行大面积烧伤削痂植皮术患者60例,ASAⅠ、Ⅱ级,随机均分成生理盐水对照组（S组）和右美托咪定组（D组）。诱导前10 min D组静脉泵注负荷量的右美托咪定（1μg/kg,10 min）。泵完以0.5μg/（kg·h）的右美托咪定维持,S组泵注相同剂量的生理盐水。两组均以氯胺酮1.0mg/kg,丙泊酚1.0mg/kg静注诱导,氯胺酮2.0-4.0mg/（kg·h）泵注复合丙泊酚2-4μg/mL靶控输注维持麻醉深度,使患者BIS值维持在46-55。观察两组诱导前（T0）、诱导后（T1）,削痂（T2）、取皮（T3）、植皮（T4）、手术结束（T5）各时间点MAP、HR变化及测定血浆肾上腺素（Epinephrine,E）、去甲肾上腺素（Norepinephrine,NE）、皮质醇（Cortisol,COR）、血糖（Glucose,GLU）含量。记录从停药到意识恢复时间、出现呼吸抑制患者例数及苏醒期发生恶心呕吐、躁动患者例数。结果与T0时相比较,S组在T2-T5时HR明显增快,MAP明显增高（P〈0.05）,且均高于D组（P〈0.05）,而D组在T0-T5HR、MAP无明显变化（P〉0.05）。在T2-T5时,S组的E、NE、COR、GLU明显高于T0时和D组（P〈0.05）。两组的意识恢复时间,出现呼吸抑制患者例数无明显差异。但是,S组术后恶心呕吐、躁动的发生率明显高于D组（P〈0.05）。结论右美托咪定可降低大面积烧伤患者静脉全麻切痂植皮术的应激反应,并且可以较少相关并发症。展开更多
文摘Extract of the eschar tissue taken from the rat which had been given full-thickness burn of20% TBSA was found to be inhibitory to the respiratory function of the mitochondria and theenergization of the inner membrane. The inhibition was also demonstrated by the 1: 2 and 1: 4and 1: 8 dilutions of the eschar extract. The extract of normal skin only showed weak inhibitory cf-fect. The 1: 4 dilution of normal skin had no (?) on mitochondrial functions. There is a widegap between the malonaldehyde (MDA) contents of these two kinds of extracts. An apparent posi-tive correlation was found between MDA content and degree of inhibition. However, on close ex-amination, it was found that the MDA content is not the direct cause of the decrease of therespiratory control ratio (RCR). The increase of MDA content is probably related to the destruc-tion of the lipid bilayer of the mitochondria. Data from the energization experiment show that theenergization process is compromised when the destruction of the membrane has proceeded to a cer-tain degrce. The inhibition of mitochondrial respiratory function becomes evident when encrgizationfunction is still normal. Therefore at least two mechanisms are operating in the inhibition process.Further work is needed to elucidate the problem.
文摘Mediterranean spotted fever(MSF)has been diagnosed clinically in the Crimean Peninsula since the 1930s.We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever,maculopapular rash,and eschar.Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 Rickettsia protein genes.The strain causing clinical illness was characterized as Rickettsia conorii subspecies conorii Malish 7.This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management.
文摘When febrile illnesses develop a rash in children, parents are very concerned about a serious disease. Many rashes associated with fever are caused by infectious diseases. Rashes are generally nonspecific and play a supportive role in differential diagnosis, but for some diseases, the appearance of the rash is essential in making a diagnosis. Here is presented the case of a 4-year-old boy with high fever, headache, abdominal pain, vomiting, diarrhea, and a generalized maculo-papular rash including palms and soles. On physical examination were found a black eschar, cervical lymphadenopathy, and hepatosplenomegaly. Laboratory findings resulted in moderate leukocytosis and moderate involvement of the liver and renal function. Based on this finding, a diagnosis of Mediterranean Spotted Fever was performed. The child recovered after medication with azithromycin. Because there is no reliable test that can confirm MSF in its early stages, the diagnosis is commonly made on the basis of clinical findings, so a high index of suspicion should be maintained while evaluating a child with fever and rash.
文摘Objective:To study epidemiology,clinical manifestations,and laboratory parameters of scrub typhus in Hadoti region of Rajasthan,India.Methods:A cross-sectional study was conducted on 50 patients with scrub typhus between August 2022 and November 2022.Scrub typhus was diagnosed by ELISA IgM scrub typhus antibody.The data about demography,vital parameters,and lab investigations were collected and analyzed.Results:Ninety-two percent of the patients were from a rural background(92%),86% were farmers and the majority were females.Most patients were 40-60 years old.The major complaints were fever(100%),myalgia(100%),headache(82%),and body swelling(80%).Major complications were pneumonia(68%),multiple organ dysfunction syndrome(62%),hepatitis(44%),and acute kidney injury(42%).Triglyceride levels were>200 mg/dL in 80% of patients,albumin<3.5 g/dL in 98% and eschar mark was present in 24%of patients.Conclusions:Scrub typhus is a serious acute febrile illness that can lead to multi-organ dysfunction and is associated with significant mortality.Increasing awareness regarding disease in endemic regions,early screening of patients,and treatment as early as possible could help prevent the patient from severe life-threatening complications.
文摘目的探讨右美托咪定对大面积烧伤患者静脉全身麻醉（全麻）切痂植皮术应激反应的影响。方法选择择期行大面积烧伤削痂植皮术患者60例,ASAⅠ、Ⅱ级,随机均分成生理盐水对照组（S组）和右美托咪定组（D组）。诱导前10 min D组静脉泵注负荷量的右美托咪定（1μg/kg,10 min）。泵完以0.5μg/（kg·h）的右美托咪定维持,S组泵注相同剂量的生理盐水。两组均以氯胺酮1.0mg/kg,丙泊酚1.0mg/kg静注诱导,氯胺酮2.0-4.0mg/（kg·h）泵注复合丙泊酚2-4μg/mL靶控输注维持麻醉深度,使患者BIS值维持在46-55。观察两组诱导前（T0）、诱导后（T1）,削痂（T2）、取皮（T3）、植皮（T4）、手术结束（T5）各时间点MAP、HR变化及测定血浆肾上腺素（Epinephrine,E）、去甲肾上腺素（Norepinephrine,NE）、皮质醇（Cortisol,COR）、血糖（Glucose,GLU）含量。记录从停药到意识恢复时间、出现呼吸抑制患者例数及苏醒期发生恶心呕吐、躁动患者例数。结果与T0时相比较,S组在T2-T5时HR明显增快,MAP明显增高（P〈0.05）,且均高于D组（P〈0.05）,而D组在T0-T5HR、MAP无明显变化（P〉0.05）。在T2-T5时,S组的E、NE、COR、GLU明显高于T0时和D组（P〈0.05）。两组的意识恢复时间,出现呼吸抑制患者例数无明显差异。但是,S组术后恶心呕吐、躁动的发生率明显高于D组（P〈0.05）。结论右美托咪定可降低大面积烧伤患者静脉全麻切痂植皮术的应激反应,并且可以较少相关并发症。
