Morroniside consists of two anomeric isomers, α-morroniside and β-morroniside. These two anomeric compounds have been separated by reversed-phase high-performance liquid chromatography(HPLC) using a gradient mobile ...Morroniside consists of two anomeric isomers, α-morroniside and β-morroniside. These two anomeric compounds have been separated by reversed-phase high-performance liquid chromatography(HPLC) using a gradient mobile phase of acetonitrile-water at 7 ℃, and verified to be anomers of morroniside by ESI-TOF-MS. The higher peak in the chromatogram was appointed to α-morroniside according to NMR data reported in previous literature. The molar ratio of α-morroniside to β-morroniside was found to be 2.855∶1 at 25 ℃. Temperature appeared to be an important factor, which affects the separation result, and a better separation was achieved at a lower temperature. The relative peak height of β-morroniside in the total ion current chromatogram measured with positive ion ESI-TOF mass spectrometer was only a half of that in UV chromatogram. This indicates that β-morroniside is less sensitive to positive ion electrospray ionization than the α-isomer.展开更多
文摘Morroniside consists of two anomeric isomers, α-morroniside and β-morroniside. These two anomeric compounds have been separated by reversed-phase high-performance liquid chromatography(HPLC) using a gradient mobile phase of acetonitrile-water at 7 ℃, and verified to be anomers of morroniside by ESI-TOF-MS. The higher peak in the chromatogram was appointed to α-morroniside according to NMR data reported in previous literature. The molar ratio of α-morroniside to β-morroniside was found to be 2.855∶1 at 25 ℃. Temperature appeared to be an important factor, which affects the separation result, and a better separation was achieved at a lower temperature. The relative peak height of β-morroniside in the total ion current chromatogram measured with positive ion ESI-TOF mass spectrometer was only a half of that in UV chromatogram. This indicates that β-morroniside is less sensitive to positive ion electrospray ionization than the α-isomer.
