[Objective]The aim was to obtain effective microsatellite markers from the public strawberry ESTs data.[Method]The distribution frequency and density of simple sequence repeats（SSRs） in strawberry EST was analyzed b...[Objective]The aim was to obtain effective microsatellite markers from the public strawberry ESTs data.[Method]The distribution frequency and density of simple sequence repeats（SSRs） in strawberry EST was analyzed by MISA（Microsatellite） software,and the redundancy was analyzed with CAP3 software.[Result]A total of 10 129 SSR sequence was received in 17 565 of EST sequences,and the distance among SSRs was about 0.90 kb,in which the Hexanucleotide repeats gained the greatest abundance,which was accounted for 61.0%,while the Trimeric,Monomeric,Dimeric,Tetrameric and Pentameric repeats accounted for 14.3%,13.1%,6.2%,4.3% and 1.1% respectively.The most abundant motif was A/T,AG,AAG and AAAG in the Monomeric,Dimeric,Trimeric and Tetrameric repeat motifs,while the CG was not found in the coding region.In these six types of repeat motifs,there was no significant difference between redundant and non redundant ESTs.[Conclusion]The availability of microsatellite markers could be expected to be achieved in the public strawberry database.展开更多
第22届欧洲胸外科医师学会(European Society of Thoracic Surgeons,ESTS)年会将于2014年6月15日至18日在丹麦首都哥本哈根的Bella会议中心举行。来自世界各地的数千名专家学者将参与这一盛会。ESTS年会历来以学术流派众多、内容全面深...第22届欧洲胸外科医师学会(European Society of Thoracic Surgeons,ESTS)年会将于2014年6月15日至18日在丹麦首都哥本哈根的Bella会议中心举行。来自世界各地的数千名专家学者将参与这一盛会。ESTS年会历来以学术流派众多、内容全面深入、形式丰富多样、前沿与实用并重而著称,目前已成为世界上普胸外科领域最顶级的盛会之一。科研时间(amegroups)编辑团队已抵达哥本哈根会议现场,继AATS现场直击后,又将为大家带来一系列ESTS大会现场报道。欢迎大家光顾丁香园英文期刊展位(AME Publishing Company:展位号A2/3-026)。先请大家关注由前线特邀客座记者,上海中山医院胸外科医生汪灏为我们带来的ESTS五大看点分享。展开更多
基金The National Basic Research Program of China(200611D100203)The Program for Changjiang Scholars and Innovative Research Teamin University,Ministry of Education of China(200558)+2 种基金The Cultivation Fund of the Key Scientific and Technical Innovation Project,Ministry of Education of China(2004-295)The National Nature Science Foundation(30671350)111 project(B07049)
基金Supported by the National Natural Science Foundation of China(30370743)Foundation of Jiangxi Normal University (2441,2732)~~
文摘[Objective]The aim was to obtain effective microsatellite markers from the public strawberry ESTs data.[Method]The distribution frequency and density of simple sequence repeats（SSRs） in strawberry EST was analyzed by MISA（Microsatellite） software,and the redundancy was analyzed with CAP3 software.[Result]A total of 10 129 SSR sequence was received in 17 565 of EST sequences,and the distance among SSRs was about 0.90 kb,in which the Hexanucleotide repeats gained the greatest abundance,which was accounted for 61.0%,while the Trimeric,Monomeric,Dimeric,Tetrameric and Pentameric repeats accounted for 14.3%,13.1%,6.2%,4.3% and 1.1% respectively.The most abundant motif was A/T,AG,AAG and AAAG in the Monomeric,Dimeric,Trimeric and Tetrameric repeat motifs,while the CG was not found in the coding region.In these six types of repeat motifs,there was no significant difference between redundant and non redundant ESTs.[Conclusion]The availability of microsatellite markers could be expected to be achieved in the public strawberry database.
文摘第22届欧洲胸外科医师学会(European Society of Thoracic Surgeons,ESTS)年会将于2014年6月15日至18日在丹麦首都哥本哈根的Bella会议中心举行。来自世界各地的数千名专家学者将参与这一盛会。ESTS年会历来以学术流派众多、内容全面深入、形式丰富多样、前沿与实用并重而著称,目前已成为世界上普胸外科领域最顶级的盛会之一。科研时间(amegroups)编辑团队已抵达哥本哈根会议现场,继AATS现场直击后,又将为大家带来一系列ESTS大会现场报道。欢迎大家光顾丁香园英文期刊展位(AME Publishing Company:展位号A2/3-026)。先请大家关注由前线特邀客座记者,上海中山医院胸外科医生汪灏为我们带来的ESTS五大看点分享。
