The present study investigated a possible mechanism for endogenous endothelin-1 (ET-1) regulation of atrial natriuretic peptide (ANP) secretion in isolated perfused acute hypoxic rabbit atria. Acute hypoxia significan...The present study investigated a possible mechanism for endogenous endothelin-1 (ET-1) regulation of atrial natriuretic peptide (ANP) secretion in isolated perfused acute hypoxic rabbit atria. Acute hypoxia significantly enhanced the release of ET-1 and the expression of the ET receptor (ETR) type A and B (ETR<sub>A</sub> and ETR<sub>B</sub>) in atrial tissues, with a concomitant increase in ANP secretion. The ETR<sub>A</sub> or ETR<sub>B</sub> antagonist, BQ123 (0.3 μmol/L) or BQ788 (0.3 μmol/L), respectively attenuated hypoxia-induced ANP secretion. Both antagonists significantly attenuated the levels of hypoxiainduced atrial phosphorylated (p)-extracellular signal-regulated kinase (ERK) and p-protein kinase B (Akt). The ERK and Akt inhibitors, PD098059 (30 μmol/L) and LY294002 (30 μmol/L), respectively mimicked the effect of the ETR antagonists. These results demonstrated that acute hypoxia- mediated atrial ET-1 regulated ANP secretion through ETR and the subsequent mitogenactivated protein kinase (MAPK)/ERK and ETR-phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathways. These pathways may mediate atrial endocrine functions under hypoxic conditions.展开更多
目的:探讨SGLT-2受体抑制剂治疗糖尿病患者的效果及对胰岛β细胞功能指数(HOMA-β)、内皮舒张功能(FMD)、内皮素(ET)及一氧化氮(NO)水平的影响。方法:选取2019年6月-2020年1月本院收治的糖尿病患者78例,依据治疗方案的不同将其分为对照...目的:探讨SGLT-2受体抑制剂治疗糖尿病患者的效果及对胰岛β细胞功能指数(HOMA-β)、内皮舒张功能(FMD)、内皮素(ET)及一氧化氮(NO)水平的影响。方法:选取2019年6月-2020年1月本院收治的糖尿病患者78例,依据治疗方案的不同将其分为对照组与研究组,每组39例。对照组采用利拉鲁肽治疗,研究组采用SGLT-2受体抑制剂治疗。对比两组干预前后HOMA-β、FMD、ET、NO、血糖指标及临床疗效。结果:干预后,两组HOMA-β与FMD均高于干预前,且研究组均高于对照组(P<0.05)。干预后,两组NO水平均高于干预前,且ET水平均低于干预前(P<0.05);干预后,研究组ET水平低于对照组,且NO水平高于对照组(P<0.05)。干预后,两组FBG、2 h PBG及HbA1c水平均低于干预前,且研究组均低于对照组(P<0.05)。研究组治疗总有效率高于对照组(P<0.05)。结论:SGLT-2受体抑制剂治疗糖尿病患者可有效提升临床效果,改善ET、HOMA-β、FMD、NO水平,有效调节血糖水平,效果理想,可考虑推广。展开更多
植物细胞受体类蛋白是一类重要的结构蛋白,在环境信号感知及细胞间信息传递中起重要作用。本研究克隆了三叶青(Tetrastigma hemsleyanum Diels et Gilg)的两个受体蛋白基因ThRLP1与ThRLK1,并对其进行了生物信息学分析、器官特异性表达...植物细胞受体类蛋白是一类重要的结构蛋白,在环境信号感知及细胞间信息传递中起重要作用。本研究克隆了三叶青(Tetrastigma hemsleyanum Diels et Gilg)的两个受体蛋白基因ThRLP1与ThRLK1,并对其进行了生物信息学分析、器官特异性表达分析和在块根发育不同时期的表达分析,以明确两基因与三叶青芽发育和块根形成的相关性。生物信息学分析表明,所克隆的两个基因中一个为细胞外受体类蛋白基因(ThR-LP1),其氨基酸序列具有特征的亮氨酸重复序列,亚细胞定位推测在细胞壁;另一个为质膜受体类蛋白激酶基因(ThRLK1),推测氨基酸序列中具有特征的丝氨酸/苏氨酸激酶的催化结构域,亚细胞定位推测在细胞核。器官特异性表达分析发现ThRLP1在块根中的表达量显著低于其他器官,ThRLK1在块根中的表达量显著低于枝蔓但高于其他器官,两个基因的表达水平并没有显示出与芽发育有相关性;块根发育不同时期表达量分析表明,ThRLP1在块根初始膨大期的表达量显著低于其他时期,ThRLK1表达量随块根发育进程而提高,ThR-LK1基因的表达与块根发育进程呈现出一定的正相关性。所克隆的两个受体蛋白可通过大肠杆菌原核表达体系正确表达出相应蛋白。本研究可为进一步明确受体类蛋白激酶基因在三叶青块根发育分子网络中的功能提供初步的参考依据。展开更多
BACKGROUND Neuroendocrine neoplasms(NENs)are a heterogeneous group of neoplasms arising from neuroendocrine cells,which contribute a small fraction of gastrointestinal malignancies.Duodenal neuroendocrine tumors(dNETs...BACKGROUND Neuroendocrine neoplasms(NENs)are a heterogeneous group of neoplasms arising from neuroendocrine cells,which contribute a small fraction of gastrointestinal malignancies.Duodenal neuroendocrine tumors(dNETs)represent 2%of all gastroenteropancreatic NENs.NENs are heterogeneous in terms of clinical symptoms,location,and prognosis.Non-functional NETs are mostly asymptomatic and need a high degree of clinical suspicion.Diagnosis of NETs is by endoscopic,endosonographic biopsy,and histopathological examination with immunohistochemistry staining for synaptophysin and chromogranin A.CASE SUMMARY We present case reports of 5 patients obtained over a period of 10 years in our center with dNETs.One patient had moderately differentiated NET and the remaining four had well-differentiated NET.Surveillance endoscopy was recommended in all the patients and is kept under regular follow-up after performing endoscopic therapy using endoscopic mucosal resection in 4 of them and one patient was advised to undergo a Whipple procedure.CONCLUSION Recently,the number of reported cases of NETs has increased due to advancements in diagnostic modalities and prevalence because of longer survival duration.The management differs based on the site,size,proliferation grade,and locally invasive pattern.They are slow-growing tumors with a good overall prognosis.The prognosis correlates with local lymph node status and metastasis.展开更多
文摘The present study investigated a possible mechanism for endogenous endothelin-1 (ET-1) regulation of atrial natriuretic peptide (ANP) secretion in isolated perfused acute hypoxic rabbit atria. Acute hypoxia significantly enhanced the release of ET-1 and the expression of the ET receptor (ETR) type A and B (ETR<sub>A</sub> and ETR<sub>B</sub>) in atrial tissues, with a concomitant increase in ANP secretion. The ETR<sub>A</sub> or ETR<sub>B</sub> antagonist, BQ123 (0.3 μmol/L) or BQ788 (0.3 μmol/L), respectively attenuated hypoxia-induced ANP secretion. Both antagonists significantly attenuated the levels of hypoxiainduced atrial phosphorylated (p)-extracellular signal-regulated kinase (ERK) and p-protein kinase B (Akt). The ERK and Akt inhibitors, PD098059 (30 μmol/L) and LY294002 (30 μmol/L), respectively mimicked the effect of the ETR antagonists. These results demonstrated that acute hypoxia- mediated atrial ET-1 regulated ANP secretion through ETR and the subsequent mitogenactivated protein kinase (MAPK)/ERK and ETR-phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathways. These pathways may mediate atrial endocrine functions under hypoxic conditions.
文摘目的:探讨SGLT-2受体抑制剂治疗糖尿病患者的效果及对胰岛β细胞功能指数(HOMA-β)、内皮舒张功能(FMD)、内皮素(ET)及一氧化氮(NO)水平的影响。方法:选取2019年6月-2020年1月本院收治的糖尿病患者78例,依据治疗方案的不同将其分为对照组与研究组,每组39例。对照组采用利拉鲁肽治疗,研究组采用SGLT-2受体抑制剂治疗。对比两组干预前后HOMA-β、FMD、ET、NO、血糖指标及临床疗效。结果:干预后,两组HOMA-β与FMD均高于干预前,且研究组均高于对照组(P<0.05)。干预后,两组NO水平均高于干预前,且ET水平均低于干预前(P<0.05);干预后,研究组ET水平低于对照组,且NO水平高于对照组(P<0.05)。干预后,两组FBG、2 h PBG及HbA1c水平均低于干预前,且研究组均低于对照组(P<0.05)。研究组治疗总有效率高于对照组(P<0.05)。结论:SGLT-2受体抑制剂治疗糖尿病患者可有效提升临床效果,改善ET、HOMA-β、FMD、NO水平,有效调节血糖水平,效果理想,可考虑推广。
文摘植物细胞受体类蛋白是一类重要的结构蛋白,在环境信号感知及细胞间信息传递中起重要作用。本研究克隆了三叶青(Tetrastigma hemsleyanum Diels et Gilg)的两个受体蛋白基因ThRLP1与ThRLK1,并对其进行了生物信息学分析、器官特异性表达分析和在块根发育不同时期的表达分析,以明确两基因与三叶青芽发育和块根形成的相关性。生物信息学分析表明,所克隆的两个基因中一个为细胞外受体类蛋白基因(ThR-LP1),其氨基酸序列具有特征的亮氨酸重复序列,亚细胞定位推测在细胞壁;另一个为质膜受体类蛋白激酶基因(ThRLK1),推测氨基酸序列中具有特征的丝氨酸/苏氨酸激酶的催化结构域,亚细胞定位推测在细胞核。器官特异性表达分析发现ThRLP1在块根中的表达量显著低于其他器官,ThRLK1在块根中的表达量显著低于枝蔓但高于其他器官,两个基因的表达水平并没有显示出与芽发育有相关性;块根发育不同时期表达量分析表明,ThRLP1在块根初始膨大期的表达量显著低于其他时期,ThRLK1表达量随块根发育进程而提高,ThR-LK1基因的表达与块根发育进程呈现出一定的正相关性。所克隆的两个受体蛋白可通过大肠杆菌原核表达体系正确表达出相应蛋白。本研究可为进一步明确受体类蛋白激酶基因在三叶青块根发育分子网络中的功能提供初步的参考依据。
文摘BACKGROUND Neuroendocrine neoplasms(NENs)are a heterogeneous group of neoplasms arising from neuroendocrine cells,which contribute a small fraction of gastrointestinal malignancies.Duodenal neuroendocrine tumors(dNETs)represent 2%of all gastroenteropancreatic NENs.NENs are heterogeneous in terms of clinical symptoms,location,and prognosis.Non-functional NETs are mostly asymptomatic and need a high degree of clinical suspicion.Diagnosis of NETs is by endoscopic,endosonographic biopsy,and histopathological examination with immunohistochemistry staining for synaptophysin and chromogranin A.CASE SUMMARY We present case reports of 5 patients obtained over a period of 10 years in our center with dNETs.One patient had moderately differentiated NET and the remaining four had well-differentiated NET.Surveillance endoscopy was recommended in all the patients and is kept under regular follow-up after performing endoscopic therapy using endoscopic mucosal resection in 4 of them and one patient was advised to undergo a Whipple procedure.CONCLUSION Recently,the number of reported cases of NETs has increased due to advancements in diagnostic modalities and prevalence because of longer survival duration.The management differs based on the site,size,proliferation grade,and locally invasive pattern.They are slow-growing tumors with a good overall prognosis.The prognosis correlates with local lymph node status and metastasis.
