Induced neural stem cells regulate microglial activation through Akt-mediated upregulation of CXCR4 and Crry in a mouse model of closed head injury

Microglial activation that occurs rapidly after closed head injury may play important and complex roles in neuroinflammation-associated neuronal damage and repair.We previously reported that induced neural stem cells can modulate the behavior of activated microglia via CXCL12/CXCR4 signaling,influencing their activation such that they can promote neurological recovery.However,the mechanism of CXCR4 upregulation in induced neural stem cells remains unclear.In this study,we found that nuclear factor-κB activation induced by closed head injury mouse serum in microglia promoted CXCL12 and tumor necrosis factor-αexpression but suppressed insulin-like growth factor-1 expression.However,recombinant complement receptor 2-conjugated Crry(CR2-Crry)reduced the effects of closed head injury mouse serum-induced nuclear factor-κB activation in microglia and the levels of activated microglia,CXCL12,and tumor necrosis factor-α.Additionally,we observed that,in response to stimulation(including stimulation by CXCL12 secreted by activated microglia),CXCR4 and Crry levels can be upregulated in induced neural stem cells via the interplay among CXCL12/CXCR4,Crry,and Akt signaling to modulate microglial activation.In agreement with these in vitro experimental results,we found that Akt activation enhanced the immunoregulatory effects of induced neural stem cell grafts on microglial activation,leading to the promotion of neurological recovery via insulin-like growth factor-1 secretion and the neuroprotective effects of induced neural stem cell grafts through CXCR4 and Crry upregulation in the injured cortices of closed head injury mice.Notably,these beneficial effects of Akt activation in induced neural stem cells were positively correlated with the therapeutic effects of induced neural stem cells on neuronal injury,cerebral edema,and neurological disorders post–closed head injury.In conclusion,our findings reveal that Akt activation may enhance the immunoregulatory effects of induced neural stem cells on microglial activation via upregulation of CXCR4 and Crry,thereby promoting induced neural stem cell–mediated improvement of neuronal injury,cerebral edema,and neurological disorders following closed head injury.

Synthetical condition assessment of long span suspension bridge based on closeness degree and FAHP

Based on the theory of pattern recognition, the concept of closeness degree between fuzzy sets is brought into the condition assessment of long span bridges. Using the fuzzy analytic hierarchy process （FAHP）, a mathematical model of a multi-objective assessment of a long span suspension bridge is set up. An example is given to show the procedure in the synthetical condition assessment of the Runyang Suspension Bridge, which includes the hierarchical division, the definition of factor weights and fuzzy membership functions, and the calculation of closeness degrees, etc. The assessment combines both the data from the health monitoring system and the manual tests. The classification of evaluation items as well as the calculation of deterministic and nondeterministic items is presented. Compared with the traditional method of point rating, this method can better describe the discreteness of monitoring data and the fuzziness in the condition assessment.

基于敏感属性值语义桶分组的t-closeness隐私模型

t-closeness模型是数据发布领域中用于抵御相似性攻击和偏斜攻击的一种有效方法,但其采用的EMD(earth mover's distance)距离没有考虑等价类与数据表间敏感属性分布的稳定性,不能全面地衡量分布间距离,在分布间稳定差异过大时会大大提高隐私泄露的风险.针对这种局限,提出了一种SABuk t-closeness模型,它在传统t-closeness模型的基础上,为更加准确地度量分布间距离,以EMD距离与KL散度(kullback-leibler divergence)结合构建距离度量标准.同时,根据敏感属性的层次树结构,对数据表进行语义相似性桶分组划分,然后采用贪心思想生成满足要求的最小等价类,并且运用k-近邻的思想来选取QI(quasi-identifiers)值相似的元组生成等价类.实验结果表明,SABuk t-closeness模型在牺牲少量时间的前提下减少了信息损失,能在有效地保护敏感信息不泄露的同时保持较高的数据效用.

Physical properties and catalog of EW-type eclipsing binaries observed byLAMOST

EW-type eclipsing binaries(hereafter called EWs)are strong interacting systems in which both component stars usually fill their critical Roche lobes and share a common envelope.Numerous EWs were discovered by several deep photometric surveys and there were about 40 785 EW-type binary systems listed in the international variable star index(VSX)by 2017 March 13.7938 of them were observed with LAMOST by 2016 November 30 and their spectral types were identified.Stellar atmospheric parameters of 5363 EW-type binary stars were determined based on good spectroscopic observations.In the paper,those EWs are cataloged and their properties are analyzed.The distributions of orbital period(P),effective temperature(T),gravitational acceleration(log(g)),metallicity([Fe/H])and radial velocity(RV)are presented for these observed EW-type systems.It is shown that about 80.6% of sample stars have metallicity below zero,indicating that EW-type systems are old stellar populations.This is in agreement with the conclusion that EW binaries are formed from moderately close binaries through angular momentum loss via magnetic braking that takes a few hundred million to a few billion years.The unusually high metallicities of a few percent of EWs may be caused by contamination of material from the evolution of unseen neutron stars or black holes in the systems.The correlations between orbital period and effective temperature,gravitational acceleration and metallicity are presented and their scatters are mainly caused by(i)the presence of third bodies and(ii)sometimes wrongly determined periods.It is shown that some EWs contain evolved component stars and the physical properties of EWs mainly depend on their orbital periods.It is found that extremely short-period EWs may be older than their long-period cousins because they have lower metallicities.This reveals that they have a longer timescale of pre-contact evolution and their formation and evolution are mainly driven by angular momentum loss via magnetic braking.

模糊t-closeness隐私保护方法研究

针对t-closeness隐私保护方法中数据泛化机制带来的信息损失量较大的不足,基于模糊理论提出一种模糊t-closeness隐私保护方法。该方法给出数据模糊化的定义,设计并实现替代泛化机制的数据模糊化过程。该过程在模糊聚类的基础上划分出符合t-closeness隐私保护要求的模糊等价类,对元素隶属度限幅求取平均值以获取元素模糊化的替代值。同时针对元素中分类属性给出模糊化语义结构树的构造方法。进行理论证明并通过实验验证模糊t-closeness隐私保护方法降低信息损失量的同时,提高了数据的隐私保护强度。

A photometric study of an EW-type binary system: GV Leo

A photometric study of a contact binary system, GV Leo is presented. New observations were done using the B VR filter bands. We find that a revised orbital period is 0.26673171 d and the orbital period of this system is decreasing at a rate of dP/ dt = -4.95 × 10-7 d yr-1. The photometric solutions are fairly well fitted at a mass ratio of q = 0.1879, with a fillout factor of f = 17.74%. The results indicate that there exists mass transfer from the more massive component to the less massive one at a rate of relative mass exchange, 6zl/m = -1.09× 10-7 yr-1. It is possible that this weak- contact system, that shows a decreasing orbital period, may undergo contraction of the inner and outer critical Roche lobes and evolve into a deep-contact binary.

Long non-coding RNA H19 regulates neurogenesis of induced neural stem cells in a mouse model of closed head injury

Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regeneration via cell replacement.However,the neural regeneration efficiency of induced neural stem cells remains limited.In this study,we explored differentially expressed genes and long non-coding RNAs to clarify the mechanism underlying the neurogenesis of induced neural stem cells.We found that H19 was the most downregulated neurogenesis-associated lnc RNA in induced neural stem cells compared with induced pluripotent stem cells.Additionally,we demonstrated that H19 levels in induced neural stem cells were markedly lower than those in induced pluripotent stem cells and were substantially higher than those in induced neural stem cell-derived neurons.We predicted the target genes of H19 and discovered that H19 directly interacts with mi R-325-3p,which directly interacts with Ctbp2 in induced pluripotent stem cells and induced neural stem cells.Silencing H19 or Ctbp2 impaired induced neural stem cell proliferation,and mi R-325-3p suppression restored the effect of H19 inhibition but not the effect of Ctbp2 inhibition.Furthermore,H19 silencing substantially promoted the neural differentiation of induced neural stem cells and did not induce apoptosis of induced neural stem cells.Notably,silencing H19 in induced neural stem cell grafts markedly accelerated the neurological recovery of closed head injury mice.Our results reveal that H19 regulates the neurogenesis of induced neural stem cells.H19 inhibition may promote the neural differentiation of induced neural stem cells,which is closely associated with neurological recovery following closed head injury.

Evaluation Indexes of Degree of Closeness between Strategy and Project Portfolio Allocation

The main activities in project portfolio allocation management are selecting the right project components given a strategy. It is crucial to establish a scientific system of evaluation indexes to guarantee the closeness between strategy and project portfolio allocation optimally. With organizations growing in sizes, the functions and objectives of project components are becoming more and more different. It is necessary to set evaluation indexes of the degree of closeness from the perspectives of financial, market share, social effects, and so on according to the strategy-oriented process of project portfolio allocation. This paper proposes a project portfolio allocation process under strategic orientation and evaluation indexes of the degree of closeness between strategy and project portfolio allocation. This will help projects managers make portfolio allocation decisions.

Design and analysis of an advanced thermal management system for the solar close observations and proximity experiments spacecraft

In this paper,the mission and the thermal environment of the Solar Close Observations and Proximity Experiments(SCOPE)spacecraft are analyzed,and an advanced thermal management system(ATMS)is designed for it.The relationship and functions of the integrated database,the intelligent thermal control system and the efficient liquid cooling system in the ATMS are elaborated upon.For the complex thermal field regulation system and extreme space thermal environment,a modular simulation and thermal field planning method are proposed,and the feasibility of the planning algorithm is verified by numerical simulation.A solar array liquid cooling system is developed,and the system simulation results indicate that the temperatures of the solar arrays meet the requirements as the spacecraft flies by perihelion and aphelion.The advanced thermal management study supports the development of the SCOPE program and provides a reference for the thermal management in other deep-space exploration programs.

Knowing one’s parents’ friends: association with parental closeness

Is one knowing about their parents’social lives associated with how close they are to them?An obvious answer might be yes.We first found that in a sample of Chinese students,they are more aware of the number of friends their mother has tham the friends their dads have.We then found that,for girls,knowing their mom and dad's friends predicts closeness with dad.Closeness to mother is not associated with knowledge of either parents’social network.For boys,however,knowing mothers’friends(but not the father’s friends)predicts their closeness to the father.As with girls,knowing either parent’s network is not associated with maternal closeness.This research implies that there are different ways closeness emerges between mothers and fathers and their children.

Research on perturbation of neutron fluence rate in a closed thermal neutron field due to medium materials

The neutron radiation field has vital applications in areas such as biomedicine,geology,radiation safety,and many others for neutron detection and neutron metrology.Correcting neutron fluence rate perturbation accurately is an important yet challenging problem.This study proposes a correction method that analyzes three physical processes.This method,which transforms the detection process from point detection to area detection,is based on a novel physical model and has been validated through theoretical analyses,experiments,and simulations.According to the average differences between the calculated and experimental results,the new method(1.67%)demonstrated better accuracy than the traditional simulation(2.17%).In a closed thermal neutron radiation field,the detector or strong neutron absorption material significantly perturbs the neutron fluence rate,whereas its impact on the energy spectrum shape and neutron directionality is relatively minor.Furthermore,based on the calculation results of the perturbation rate formula for medium materials with different compositions and sizes,the larger the volume and capture cross section of the medium,the higher the perturbation rate generated in the closed radiation field.

Factors Associated with Closely Spaced Pregnancies at the Departmental Hospital Centre of Zou-Collines (Benin) in 2022

Introduction: Birth spacing to prevent closely-spaced pregnancies, which are a source of maternal and neonatal morbidity and mortality, is not always respected by our women, as recommended by the World Health Organization (WHO). The objective was to study the factors associated with closely-spaced pregnancies among women followed at the Departmental Hospital Center du Zou et du Colline (DHC/Z-C) in 2022. Study Method: This was a descriptive, analytical cross-sectional study with prospective data collection from 20 May to 30 June 2022. Results: At the end of the study, 83 of the 117 women surveyed had close pregnancies, a frequency of 70.94%. They had an average age of 27.0 ± 6.3 years and were of Fon ethnicity and related in 91.57% of cases. The majority were married (96.60%). The factors significantly associated with these closely spaced pregnancies were age between 25 and 30 years, household occupation, age at first birth, age at marriage, women’s perception of contraceptive method (CM), women’s knowledge of the existence of a center providing family planning services and non-use of MC. Conclusion: Close pregnancies are still very common in the commune of Abomey. It is therefore important to increase the use of contraceptive methods in this part of the country.

On Fuzzy Regularly Closed Filters in Michálek Fuzzy Topological Space

C.L. Chang’s introduction of fuzzy topology in 1981 opened up new avenues for parallel theories in topology. However, Chang’s work appears to focus more on the topology of fuzzy sets rather than fuzzy topology itself. In 1975, Michálek presented a functional definition of ordinary topology and later developed fuzzy topology as a distinct extension of this idea, setting it apart from Chang’s approach. While there has been significant research on Chang’s fuzzy topology, Michálek’s version has not received as much attention. This paper introduces the concept of fuzzy regularly closed filters, or FRCM filters, within Michálek’s fuzzy topological space and explores some properties of FRCM ultrafilters.

Formation of a Rapidly Rotating Classical Be-star in a Massive Close Binary System

This paper investigates the spin-up of a mass-accreting star in a close binary system passing through the first stage of mass exchange in the Hertzsprung gap. Inside an accreting star, angular momentum is carried by meridional circulation and shear turbulence. The circulation carries part of the angular momentum of the accreted layers to the accretor's surface. The greater the rate of arrival of angular momentum in the accretor is, the greater this part. It is assumed that this part of the angular momentum can be removed by the disk further from the accretor. If the angular momentum in the matter entering the accretor is more than half the Keplerian value, then the angular momentum obtained by the accretor during mass exchange stage does not depend on the rate of arrival of angular momentum. The accretor may have the characteristics of a Be-star immediately after the end of mass exchange.

Sensing-Communication-Computing-Control Closed Loop for NOMA-UAV Systems

In the areas without terrestrial communication infrastructures,unmanned aerial vehicles(UAVs)can be utilized to serve field robots for mission-critical tasks.For this purpose,UAVs can be equipped with sensing,communication,and computing modules to support various requirements of robots.In the task process,different modules assist the robots to perform tasks in a closed-loop way,which is referred to as a sensing-communication-computing-control(SC3)loop.In this work,we investigate a UAV-aided system containing multiple SC^(3)loops,which leverages non-orthogonal multiple access(NOMA)for efficient resource sharing.We describe and compare three different modelling levels for the SC^(3)loop.Based on the entropy SC^(3)loop model,a sum linear quadratic regulator(LQR)control cost minimization problem is formulated by optimizing the communication power.Further for the assure-to-be-stable case,we show that the original problem can be approximated by a modified user fairness problem,and accordingly gain more insights into the optimal solutions.Simulation results demonstrate the performance gain of using NOMA in such task-oriented systems,as well as the superiority of our proposed closed-loop-oriented design.

Design and Test of Closed Hydraulic Transmission System of 4WD High-clearance Wheeled Sprayer

In conjunction with the working characteristics of the high-clearance wheeled sprayer and the benefits of the closed hydraulic system,a series of reasonable working parameters should be established,and a hydraulic system that fulfills the requisite specifications should be designed.The AMESim software model is employed to construct a closed hydraulic transmission system,and the simulation analysis is then performed according to the data of hydraulic components.According to analysis results,the prototype can be optimized and upgraded,and a verification test is further carried out.The test results demonstrate that the designed closed hydraulic transmission system meets the actual working requirements of the high-clearance wheeled sprayer and provides a stable experimental platform for intelligent control of agricultural machinery.

Simulation Modelling and Techno-Economics of Supercritical Carbon Dioxide Recompression Closed Brayton Cycle

In recent years, there has been global interest in meeting targets relating to energy affordability and security while taking into account greenhouse gas emissions. This has heightened major interest in potential investigations into the use of supercritical carbon dioxide (sCO2) power cycles. Climate change mitigation is the ultimate driver for this increased interest;other relevant issues include the potential for high cycle efficiency and a circular economy. In this study, a 25 MWe recompression closed Brayton cycle (RCBC) has been assessed, and sCO2 has been proposed as the working fluid for the power plant. The methodology used in this research work comprises thermodynamic and techno-economic analysis for the prospective commercialization of this sCO2 power cycle. An evaluated estimation of capital expenditure, operational expenditure, and cost of electricity has been considered in this study. The ASPEN Plus simulation results have been compared with theoretical and mathematical calculations to assess the performance of the compressors, turbine, and heat exchangers. The results thus reveal that the cycle efficiency for this prospective sCO2 recompression closed Brayton cycle increases (39% - 53.6%) as the temperature progressively increases from 550˚C to 900˚C. Data from the Aspen simulation model was used to aid the cost function calculations to estimate the total capital investment cost of the plant. Also, the techno-economic results have shown less cost for purchasing equipment due to fewer components being required for the cycle configuration as compared to the conventional steam power plant.

基于k-means++的多敏感属性t-closeness隐私保护

隐私保护是数据共享的主要关注点之一,特别是当涉及个人敏感信息时。k匿名是传统的微数据隐私保护模型,存在同质泄露问题。t-closeness是k匿名的扩展方法之一,它限制了属性披露的风险,并提供了严格的隐私保证,现有的t-closeness工作主要针对单敏感属性,并没有考虑到多敏感属性。本文提出一种基于聚类的多敏感属性t-closeness算法,首先用k-means++算法依据敏感属性将数据集划分成k个分组,然后分别从每个簇中抽取一个样本以生成满足t-closeness的等价类。为提高隐私保护程度,扫描所有等价类,将不满足t-closeness的等价类合并到质心与其最近的等价类中。实验表明,我们的方法具有良好效果。

Study on the Effect of Behavioral Interventions Based on the Theory of“Knowledge,Attitude,Practice”on Tuberculosis Cognitive Level of Close Contacts

Objective:Based on the theory of“Knowledge,Attitude,Practice,”health education was carried out for close contact with tuberculosis(TB)at a university in Beijing,and its impact on the students’awareness of TB was studied.This study could provide a reference for effective health education interventions among close contacts of TB in undergraduates.Methods:102 undergraduates who had close contact with one confirmed case of pulmonary TB in April 2021 at a university were randomly divided into a control group and an intervention group.The intervention group was given the“Knowledge,Attitude,Practice”health education intervention by professionally trained doctors,and were asked to complete a self-designed TB-related questionnaire before and after the intervention.Both groups performed the Tuberculin Skin Test(TST)and chest X-ray(CR)examination.Results:The rate of the TST test was 18.6%,and CR examination showed 0 cases of pulmonary TB.The survey results showed that the TB awareness rate(96.1%,49/51)in the intervention group was significantly higher than that of the control group(51.0%,26/51),and the differences in awareness rate between the two groups were statistically significant(P<0.05).Regarding the attitude/skills toward TB,the prevalence rate of tuberculosis-related attitude/behavior in the intervention group(94.1%,47/51)was significantly higher than that of the control group(60.8%,31/51),and the differences between the two groups were statistically significant(P<0.05).Conclusion:Both self-controlled and parallel-controlled studies showed that the health education intervention of“Knowledge,Attitude,Practice”improved the awareness of TB among close contacts of TB in undergraduates.