电子邮件自动分拣一法

常见的E-mail软件没有分拣功能,来自不同信箱的邮件统统放入"收件箱".如果能根据信箱账号等信息,把邮件分类放入不同文件夹,将有助于大量邮件的管理.最新的Outlook Express 5提供了按规则自动分拣邮件的功能.

实用软件技巧荟萃

邮件出现乱码的解决办法 首先用Outlook Express将乱码的邮件收取下来，然后打开邮件，选择View(查看)→Encoding(编码)，然后调整其下的编码设置，比如选择Chinese Simple(简体中文)、Chinese Traditional(繁体中文)、中文HZ或Unicode试试看。一般情况，绝大多数乱码的邮件都可以转成中文。如果你觉得这样很麻烦，可以将GB2312设置为默认的字体，方法如下(以Outlook Express5为例)：通过“工具选项→阅读→字体”，在“编码”处选好GB2312，然后选“设为默认值”。

Relationship between RGS5 expression and differentiation and angiogenesis of gastric carcinoma

AIM:To explore the regulator of G-protein signaling 5 (RGS5) expression in gastric carcinoma and its association with differentiation and microvascular density (MVD).METHODS:Expression of RGS5 and CD34 were examined in 76 cases of gastric carcinoma,including 22 cases with lymph node metastasis and 54 cases without lymph node metastasis determined by immunohistochemistry (IHC).MVD was assessed using CD34 monoclonal antibody.The presence of RGS5 and CD34 was analyzed by IHC using the Envision technique.RESULTS:The RGS5 expression in gastric carcinoma was positively correlated with the differentiation of the tumor (r=0.345,P < 0.001),but not related with age,gender,tumor size,clinical stage and lymph node metastasis (P > 0.05).The average MVD in the group with lymph node metastasis was significantly higher than that in the group without lymph node metastasis (P < 0.05).RGS5 expression was negatively correlated with the average MVD (P < 0.05).CONCLUSION:RGS5 expression level in gastric carcinoma is associated with the differentiation and MVD of the tumor,and may be used as an important parameter for determining the prognosis of gastric carcinoma patients.

Mutations of t-complex testis expressed gene 5 transcripts in the testis of sterile t-haplotype mutant mouse

Aim： To determine the possible roles of the t-complex testis expressed gene 5 （Tctex5） on sperm functions, the fulllength sequence of mRNA was studied and compared in the testis between the normal wild-type and the sterile t-haplotype mutant mice. Methods： We applied rapid amplification of cDNA ends, Northern blot and reverse transcription polymerase chain reaction to analyze the full length of Tctex5 mRNAs isolated from testes of the wild-type and the t-haplotype mice. Reverse transcription polymerase chain reaction was used to semi-quantitatively compare expression of Tctex5 transcripts in the 16 tissues and 9.5 day stage embryos in the wild-type mice. E-translation was applied to estimate the amino acid sequences. Results： One long and one short transcript of Tctex5 mRNA were discovered in mouse testis of wild-type （Tctex5^long-＋ and Tctex5^short-＋） and t-haplotype （Tctex5^long-＋ and Tctex5^short-＋） mice, respectively. Being enhanced only in the testis, Tctex5^long-＋ had 17 point mutations and one 15-bp-deletion in the exon 1 region, comparing with the Tctex5^long-＋, whereas the Tctex5^short-＋ was similar to the Tctex5^short-＋. The short isoforms of Tctex5 mRNAs in the two models encoded exactly the same peptides, but the long isoforms did not. The estimated peptide encoded by Tctex5^long-＋ had significant mutations on putative sites of phosphorylation and PP1 binding. Conclusion： We established that mutations that occur in the Tctex5 long transcript of the t-haplotype mice are important for normal sperm function, whereas the short transcript of Tctex5 might have a conserved function among different tissues. （Asian J Androl 2008 Mar; 10： 219-226）

问题解答

液晶显示器在技术方面有什么特点，为什么价格那么贵，难道没有适合家用的产品吗?

5-aza-2'-deoxycitydine induces demethylation and up-regulates transcription of p16^(INK4A) gene in human gastric cancer cell lines

Background To investigate the effects of DNA methylation on the expression of tumor-associated genes and the cell cycle in human gastric cancer cells. Methods Two gastric cancer cell lines (MKN-45 and HGC-27) were treated with DNA methyltransferase (DNMT) inhibitor,5-aza-2’-deoxycytidine (5-aza-dC). The expressions of p16 INK4A,p21 WAF1,p53 , p73 ,c-Ha-ras and c-myc genes mRNA were detected by using reverse transcription PCR (RT-PCR). DNA methylation status of p16 INK4A gene promoter was assayed by bisulfite modification and sequencing. The cell cycle was analyzed by using flow cytometry (FCM). Results 5-aza-dC induced the demethylation of p16 INK4A gene promoter. The expression of p16 INK4A mRNA was obviously up-regulated by treatment with 10 μmol/L (MKN-45 cells) or 5 μmol/L (HGC-27 cells) of 5-aza-dC for 24 hours. However,5-aza-dC treatment failed to regulate the expressions of p21 WAF1,p53 , p73 ,c-Ha-ras and c-myc genes in MKN-45 and HGC-27 cells. Furthermore,5-aza-dC induced the cell cycle arrest in G1 phase in HGC-27 cell,but not in MKN-45 cell. Conclusions DNA methylation regulates the transcription of p16 INK4A but not p21 WAF1 and proto-oncogenes in human gastric cancer cell lines MKN-45 and HGC-27.

Pattern analysis of stem cell differentiation during in vitro Arabidopsis organogenesis

Plant somatic cells have the capability to switch their cell fates from differentiated to undifferen-tiated status under proper culture conditions,which is designated as totipotency.As a result,plant cells can easily regenerate new tissues or organs from a wide variety of explants.However,the mechanism by which plant cells have such remarkable regeneration ability is still largely unknown.In this study,we used a set of meristem-specific marker genes to analyze the patterns of stem cell differentiation in the processes of somatic embryogenesis as well as shoot or root organogenesis in vitro.Our studies furnish preliminary and important information on the patterns of the de novo stem cell differentiation during various types of in vitro organogenesis.