Platelet-activating factor (PAF) exhibits a variety of biological activities and it be thought to involved in various pathophysiological process. In this paper, some studies were summarized about those roles of PAF in...Platelet-activating factor (PAF) exhibits a variety of biological activities and it be thought to involved in various pathophysiological process. In this paper, some studies were summarized about those roles of PAF in a variety productive processes of female of mammalian that include fertilization, implantation and parturition, and that was involved in the concentration, degradation and some assay methods of PAF. The relationship between PAF and early pregnancy factor(EPF was reviewed.展开更多
Early pregnancy factor (EPF) was purified from the pooled sera of 21 0 pregnant women at 3- 8 weeks of gestation. Sera from healthy nonpregnant women were used as control. The samples (G-Ⅱ . G-Ⅲ and G-Ⅳ) obtained f...Early pregnancy factor (EPF) was purified from the pooled sera of 21 0 pregnant women at 3- 8 weeks of gestation. Sera from healthy nonpregnant women were used as control. The samples (G-Ⅱ . G-Ⅲ and G-Ⅳ) obtained from pregnant women had EPF activity but no HCG activity. Polyacrylamide gel electrophoresis showed that the major bands in pregnant G-Ⅲ and G-Ⅳ were at similar positions in tube gels. The results of SDS-PAGE showed 3 bands in pregnant G-Ⅳ: 57. 0 kD. 38. 0 kD and 19. 0 kD. The basic active form of EPF may be a small peptide of 1 9. 0 kD. The isoelectric points of pregnant G-Ⅳ were 6. 45 and 8. 20.展开更多
文摘Platelet-activating factor (PAF) exhibits a variety of biological activities and it be thought to involved in various pathophysiological process. In this paper, some studies were summarized about those roles of PAF in a variety productive processes of female of mammalian that include fertilization, implantation and parturition, and that was involved in the concentration, degradation and some assay methods of PAF. The relationship between PAF and early pregnancy factor(EPF was reviewed.
文摘Early pregnancy factor (EPF) was purified from the pooled sera of 21 0 pregnant women at 3- 8 weeks of gestation. Sera from healthy nonpregnant women were used as control. The samples (G-Ⅱ . G-Ⅲ and G-Ⅳ) obtained from pregnant women had EPF activity but no HCG activity. Polyacrylamide gel electrophoresis showed that the major bands in pregnant G-Ⅲ and G-Ⅳ were at similar positions in tube gels. The results of SDS-PAGE showed 3 bands in pregnant G-Ⅳ: 57. 0 kD. 38. 0 kD and 19. 0 kD. The basic active form of EPF may be a small peptide of 1 9. 0 kD. The isoelectric points of pregnant G-Ⅳ were 6. 45 and 8. 20.
