Dominant early heading(DEH)in rice(Oryza sativa L.)is of interest in both breeding and genetics.The genetic mechanisms underlying DEH have remained largely unclear.We have developed a near-isogenic DEH line without yi...Dominant early heading(DEH)in rice(Oryza sativa L.)is of interest in both breeding and genetics.The genetic mechanisms underlying DEH have remained largely unclear.We have developed a near-isogenic DEH line without yield drag named DEH_229 by sister-line backcross(BC)breeding with MH63,a restorer,as the genetic background.We conducted a pilot genetic investigation under both short-day(SD)and long-day(LD)conditions.The DEH line harbored only 1.06%variation in the genome sequence relative to MH63.The variants were distributed throughout the genome.Using QTL mapping by sequencing(QTL-seq)on an F_(2) population derived from a cross of MH63×DEH_229,57 loci were detected under the SD condition.Joint mapping employing a genome-wide association study with accessions from the 3000 rice genome sequencing project(3K-RG),reduced the number of QTL by 43.9%.Using Rice Functional Genomics&Breeding(RFGB)database,the number of SNP cluster regions within the QTL regions reduced by 27.3%.Further comparison of the genome variation between DEH_229 and MH63 in addition to gene annotation information revealed a new DEH allele of DTH3 with multiple variable sites as a possible major factor underlying the early-heading phenotype of DEH_229.An InDel marker,ZMEH_1,was designed based on the variation between DEH_229 and MH63 within this region.It accounted for 86.0%of heading date variation under both SD and LD conditions in 109 randomly chosen progeny derived from extreme lines of the MH63×DEH_229 population.This study reveals the genetic complexity of DEH in the near-isogenic line and may provide useful material and marker information for plant molecular breeding.展开更多
本研究报道了一个来自EMS诱变优良恢复系浙恢7954的新水稻早衰突变体lst(Leaf senescence at tillering stage),其最主要的表型特点是叶片上出现锈色衰老斑,通过荧光共聚焦倒置显微镜观察和透射电子显微镜镜观察表明lst的叶绿素荧光减弱...本研究报道了一个来自EMS诱变优良恢复系浙恢7954的新水稻早衰突变体lst(Leaf senescence at tillering stage),其最主要的表型特点是叶片上出现锈色衰老斑,通过荧光共聚焦倒置显微镜观察和透射电子显微镜镜观察表明lst的叶绿素荧光减弱,叶绿体结构异常。生理生化分析发现lst突变体剑叶在早衰性状出现时,与野生型相比,其叶绿素和类胡萝卜素含量显著降低;O2-、H2O2、MDA含量升高,保护酶系统SOD和CAT活性降低。遗传分析表明,该突变体受1对隐性核基因控制,利用200株日本晴/lst的F2隐性定位群体,最终将LST基因定位在第3染色体SSR标记RM3646和InDel标记IAC120537-1,2之间,共171kb,含两个BAC,30个基因,这为基因的克隆和功能研究奠定了基础。展开更多
基金We appreciate supports from the National Key Research and Development Program of China(2016YFD0101801)the National Natural Science Foundation of China(31871715)+2 种基金the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ICS2020YJ07BX,1610092015003-10,and Y2020PT24)the Open Program from the Guangxi Key Laboratory of Rice Genetics and Breeding(2018-05-Z06-KF01)the“Green Super Rice”Project from Bill&Melinda Gates’Foundation(OPP1130530).
文摘Dominant early heading(DEH)in rice(Oryza sativa L.)is of interest in both breeding and genetics.The genetic mechanisms underlying DEH have remained largely unclear.We have developed a near-isogenic DEH line without yield drag named DEH_229 by sister-line backcross(BC)breeding with MH63,a restorer,as the genetic background.We conducted a pilot genetic investigation under both short-day(SD)and long-day(LD)conditions.The DEH line harbored only 1.06%variation in the genome sequence relative to MH63.The variants were distributed throughout the genome.Using QTL mapping by sequencing(QTL-seq)on an F_(2) population derived from a cross of MH63×DEH_229,57 loci were detected under the SD condition.Joint mapping employing a genome-wide association study with accessions from the 3000 rice genome sequencing project(3K-RG),reduced the number of QTL by 43.9%.Using Rice Functional Genomics&Breeding(RFGB)database,the number of SNP cluster regions within the QTL regions reduced by 27.3%.Further comparison of the genome variation between DEH_229 and MH63 in addition to gene annotation information revealed a new DEH allele of DTH3 with multiple variable sites as a possible major factor underlying the early-heading phenotype of DEH_229.An InDel marker,ZMEH_1,was designed based on the variation between DEH_229 and MH63 within this region.It accounted for 86.0%of heading date variation under both SD and LD conditions in 109 randomly chosen progeny derived from extreme lines of the MH63×DEH_229 population.This study reveals the genetic complexity of DEH in the near-isogenic line and may provide useful material and marker information for plant molecular breeding.
基金中央高校基本科研业务费(XDJK2013A023)国家自然科学基金项目(31171178)资助+1 种基金supported by the Fundamental Research Funds for the Central Universities(XDJK2013A023)the National Natural Science Foundation of China(31171178)
文摘本研究报道了一个来自EMS诱变优良恢复系浙恢7954的新水稻早衰突变体lst(Leaf senescence at tillering stage),其最主要的表型特点是叶片上出现锈色衰老斑,通过荧光共聚焦倒置显微镜观察和透射电子显微镜镜观察表明lst的叶绿素荧光减弱,叶绿体结构异常。生理生化分析发现lst突变体剑叶在早衰性状出现时,与野生型相比,其叶绿素和类胡萝卜素含量显著降低;O2-、H2O2、MDA含量升高,保护酶系统SOD和CAT活性降低。遗传分析表明,该突变体受1对隐性核基因控制,利用200株日本晴/lst的F2隐性定位群体,最终将LST基因定位在第3染色体SSR标记RM3646和InDel标记IAC120537-1,2之间,共171kb,含两个BAC,30个基因,这为基因的克隆和功能研究奠定了基础。
