Purpose:To investigate the mechanism and sequence of formation of ring-shaped mitochondria in retinal pigment epithelial ce3lls of a chick model of gyrate atro-phy.Methods:Electron microscopic analysis of the ultrastr...Purpose:To investigate the mechanism and sequence of formation of ring-shaped mitochondria in retinal pigment epithelial ce3lls of a chick model of gyrate atro-phy.Methods:Electron microscopic analysis of the ultrastructure of retinal pigment epithelial(RPE)mitochondria was carried out in chicks injected intravitreally with formoguanamine regularly(every4days)over the first 2weeksor4weeks post-hatching.Formoguanamine is a triazine drug which induces hyperor-nithinemic symptoms in the chick eye similar to those seen in human gyrate atro-phy.Results:A large population of irregularly shaped mitochondria was observed in the RPE of both peripheral and central retina.They showed extensive morpholog-ical changes.At 2wk,the mitochondria appeared enlarged and abnormal in shape with vacuolisation,partial loss of their double membrane and reduced mitochon-drial cristae.By 4wk,the mitochondria had assumed a rounder,almost circular profile,many with central holes,so-called ring mitochondria.Conclusion:The appearance of ring-shaped mitochondria has been previously as-cribed to the section of cupshaped three-dimensional structures.We present evi-dence that ring-shaped mitochondrial profiles arise through at least two different mechanisms of membrane breakdown and intraorganelle vacuoolisation.The nature of the three dimensional structures of these abnormal mit.展开更多
AIMTo report the appearance of choriocapillaris atrophy after combined high dose intravitreal triamcinolone acetonide (TA) and photodynamic therapy (PDT) to treat choroidal neovascularization (CNV) associated with age...AIMTo report the appearance of choriocapillaris atrophy after combined high dose intravitreal triamcinolone acetonide (TA) and photodynamic therapy (PDT) to treat choroidal neovascularization (CNV) associated with age related macular degeneration (AMD).展开更多
基金a grant from the Australian Research Council (to DPC and SGC) a grant from the Australian Retinitis Pigmentosa Association.
文摘Purpose:To investigate the mechanism and sequence of formation of ring-shaped mitochondria in retinal pigment epithelial ce3lls of a chick model of gyrate atro-phy.Methods:Electron microscopic analysis of the ultrastructure of retinal pigment epithelial(RPE)mitochondria was carried out in chicks injected intravitreally with formoguanamine regularly(every4days)over the first 2weeksor4weeks post-hatching.Formoguanamine is a triazine drug which induces hyperor-nithinemic symptoms in the chick eye similar to those seen in human gyrate atro-phy.Results:A large population of irregularly shaped mitochondria was observed in the RPE of both peripheral and central retina.They showed extensive morpholog-ical changes.At 2wk,the mitochondria appeared enlarged and abnormal in shape with vacuolisation,partial loss of their double membrane and reduced mitochon-drial cristae.By 4wk,the mitochondria had assumed a rounder,almost circular profile,many with central holes,so-called ring mitochondria.Conclusion:The appearance of ring-shaped mitochondria has been previously as-cribed to the section of cupshaped three-dimensional structures.We present evi-dence that ring-shaped mitochondrial profiles arise through at least two different mechanisms of membrane breakdown and intraorganelle vacuoolisation.The nature of the three dimensional structures of these abnormal mit.
文摘AIMTo report the appearance of choriocapillaris atrophy after combined high dose intravitreal triamcinolone acetonide (TA) and photodynamic therapy (PDT) to treat choroidal neovascularization (CNV) associated with age related macular degeneration (AMD).
