Clonal isolation of endothelial colony-forming cells from early gestation chorionic villi of human placenta for fetal tissue regeneration

BACKGROUND Endothelial colony-forming cells(ECFCs)have been implicated in the process of vascularization,which includes vasculogenesis and angiogenesis.Vasculogenesis is a de novo formation of blood vessels,and is an essential physiological process that occurs during embryonic development and tissue regeneration.Angiogenesis is the growth of new capillaries from pre-existing blood vessels,which is observed both prenatally and postnatally.The placenta is an organ composed of a variety of fetal-derived cells,including ECFCs,and therefore has significant potential as a source of fetal ECFCs for tissue engineering.AIM To investigate the possibility of isolating clonal ECFCs from human early gestation chorionic villi(CV-ECFCs)of the placenta,and assess their potential for tissue engineering.METHODS The early gestation chorionic villus tissue was dissociated by enzyme digestion.Cells expressing CD31 were selected using magnetic-activated cell sorting,and plated in endothelial-specific growth medium.After 2-3 wks in culture,colonies displaying cobblestone-like morphology were manually picked using cloning cylinders.We characterized CV-ECFCs by flow cytometry,immunophenotyping,tube formation assay,and Dil-Ac-LDL uptake assay.Viral transduction of CVECFCs was performed using a Luciferase/tdTomato-containing lentiviral vector,and transduction efficiency was tested by fluorescent microscopy and flow cytometry.Compatibility of CV-ECFCs with a delivery vehicle was determined using an FDA approved,small intestinal submucosa extracellular matrix scaffold.RESULTS After four passages in 6-8 wks of culture,we obtained a total number of 1.8×107 CV-ECFCs using 100 mg of early gestational chorionic villus tissue.Immunophenotypic analyses by flow cytometry demonstrated that CV-ECFCs highly expressed the endothelial markers CD31,CD144,CD146,CD105,CD309,only partially expressed CD34,and did not express CD45 and CD90.CV-ECFCs were capable of acetylated low-density lipoprotein uptake and tube formation,similar to cord blood-derived ECFCs(CB-ECFCs).CV-ECFCs can be transduced with a Luciferase/tdTomato-containing lentiviral vector at a transduction efficiency of 85.1%.Seeding CV-ECFCs on a small intestinal submucosa extracellular matrix scaffold confirmed that CV-ECFCs were compatible with the biomaterial scaffold.CONCLUSION In summary,we established a magnetic sorting-assisted clonal isolation approach to derive CV-ECFCs.A substantial number of CV-ECFCs can be obtained within a short time frame,representing a promising novel source of ECFCs for fetal treatments.

Effect of Mifepristone on Transforming Growth Factor -βand its Receptor Transcription in Decidua, Villi and Serum Tumor Necrosis Factor -α Level in Early Human Gestation

Objective To investigate the role of mifepristone in regulating cytokines of materno-fetal interface and serum of human early gestationMethods Thirty-five women with early pregnancy received mifepristone 50 mg orallyon study d 1 and d 2, respectively, followed by undergoing artificial abortion to getdecidua and villi on study d 3. Twenty-five women with early pregnancy withoutmifepristone administration as control also underwent artificial abortion to get de-cidua and villi. The expressions of TGF-β1 and TGF-β1 receptor mRNA in the earlydecidua and villi were assessed by using RT-PCR . The concentrations of serum TNF-α were measured by radioimmunoassay.Results The decidual expressions of TGF- β1 mRNA and TGF-β1 receptor mRNA in thetreated group were significantly lower than those of the control (P<0.05), while thevillus expressions of TGF-β1 and TGF-β1 receptor mRNA in the treated group were notsignificantly different from those of the control (P>0.05). The serum TNF-β1 levelselevated significantly after mifepristone treatment.Conclusion The antigestational effect of mifepristone might act through suppressingthe transcription of TGF-β1 and TGF-β1 receptor in the decidua and increasing theserum TNF-α level, which interfered in the materno-fetal interface Th2 bias.

A Srudy on Ultrastructures of Normal 5-8 Week Human Placental Villi

Fourteen normal human placental villi at a fetal age of 5-8 weeks were examined withelectron microscope. The results were vide infra.(1)The cytotrophoblasts were characterized by largenucleus with less heterochromatin and prominent nucleolus with a number of free polyribosomes as well asscattered mitochondria in the cytoplasm.(2) There was a layer of dense microvilli on the surface of thesyncytiotrophoblastic cells.The size and shape of microvilli were variable. Usually fine globular expansions were present at the apical pole of microvilli and the cell membrane between the base of microvillihad invaginations into the cytoplasm forming small vesicles and tubules. Rough endoplasmic reticulum,free polyribosomes,mitochondria, lipid droplets and few myelinlike figures were also found in the cytoplasm.Part of cytoplasm penetrated into the interspaces of cytotrophoblastic cells and became in directcontact with the basement membrane.(3) Numerous desmosomes were seen between the cells of cytotrophoblast and syncytiotrophoblast.And (4) in the strom a of villi,mesenchymal cells were abundant inrough endoplasmic reticulum and well developed Golgi complex. Mesenchymat cells were markedlyfunctionally active. Ultrastructural characteristics of placental villin in connexion with their functionwere discussed.

The contents and effect of angiotensin Ⅱ on hCG release in human placental villi during the first trimester of gestation

ＴｈｅｃｏｎｔｅｎｔｓａｎｄｅｆｆｅｃｔｏｆａｎｇｉｏｔｅｎｓｉｎⅡｏｎｈＣＧｒｅｌｅａｓｅｉｎｈｕｍａｎｐｌａｃｅｎｔａｌｖｉｌｌｉｄｕｒｉｎｇｔｈｅｆｉｒｓｔｔｒｉｍｅｓｔｅｒｏｆｇｅｓｔａｔｉｏｎＷａｎｇＨｏｎｇ（王红），ＺｏｕＡｉｍｉｎ（邹爱...

High Expression of hsMAD2 in the Villi of Spontaneously Aborted Embryo with Chromosomal Abnormality

Objective: To investigate the changes of hsMAD2 protein and gene expression levels during chromosome segregation of human embryos. Method: The embryos of spontaneous abortion were collected in our hospital from 2009 to 2013, the chromosomal numbers of the embryonic villi were subsequently detected by fluorescence in situ hybridization (FISH). The patients were then divided into the normal and abnormal groups based on the chromosome number. The hsMAD2 protein and gene expression levels in the villi tissues of the two embryo groups were detected by western blotting and qRT-PCR, respectively. The hsMAD2 protein and gene levels in the embryonic villus tissue of the patient were detected. Results: From 2009 to 2013, we collected 50 embryos from spontaneous abortion patients. The chromosome abnormality and no abnormality were 36 cases (abnormal number of 28 cases (56.0%) and chimerism in 8 cases (16.0%)) and 14 cases (28.0%), respectively. The expression of hsmad2 protein and its gene in the villi of spontaneously aborted embryo with chromosomal abnormality in the abnormal group was significantly higher than that in those without chromosomal abnormalities (0.88 ± 0.20 vs 0.61 ± 0.19, P < 0.05), (23.46 ± 0.07 vs 18.35 ± 0.10, P < 0.05). Conclusion: Abnormal number of chromosomes is closely related to spontaneous abortion Linked, hsMAD2 factor has a card effect on the cell cycle, can block the mitotic process of cells, and play an important role in maintaining the normal separation and stability of chromosomes.

The physiological characteristics of the basal microvilli microvessels in pancreatic cancers

Pancreatic cancer(PC)is a highly lethal tumor with controversial high glucose uptake and hypomicrovascularity.And,the hypomicrovasculature,which is considered to have poor perfusion,blocks the delivery of drugs to tumors.Previously,we described the preferential existence of a novel endothelial projection with trafficking vesicles in PCs,referring to basal microvilli.However,whether the basal microvilli microvessels have good perfusion or deliver nutrients to the tumor milieu is unknown.Here,we perfused multiple endothelial markers and nutrients to autochthonous PC-bearing mice to study the nutrient trafficking and perfusion status of the basal microvilli microvasculature.

The Expression of Integrin β_3 and Intercellular Adhesion Molecule(ICAM-1) in Decidua and Chorionic Villi during Mifepristone Induced Abortion

The effects of mifepristone with misoprostol on the expression of the integrin β 3 and intercellular adhesion molecule 1 (ICAM 1) in decidua and chorionic villi tissues in early pregnancy in 10 cases were investigated by immuno flow cytometry (the experiment group). At the same time, the other 10 cases induced by mechanical vacuum aspiration were collected as the control. The results showed that, the positive rate of integrin β 3 and ICAM 1 in decidua of the experiment group were 19.1±5.01% and 20.61 ±6.51%; while those in chorionic villi were 21.32±4.38% and 20.29± 6.49%, which were significantly lower than those in the control group. These results suggested that integrin β 3 and ICAM 1 may take part in the maintenance of early pregnancy. The mechanism of mifepristone induced abortion may be mediated by the down regulation of the integrin β 3 and ICAM 1 expression in decidua and chorionic villi.

Expression and molecular mechanism of PCNA,Caspase-3,IL-6 and Survivin proteins in chorionic villi and decidual tissue of early embryo damage

Objective:This study aims to explore its role in embryo damage and the relationship between them by testing the expression of PCNA,Caspase-3,IL-6 and Survivin protein in the chorionic villi and decidual tissue of patients with unexplained early embryo damage and normal early pregnancy at the same time voluntarily requested uterine aspiration abortion in order to clarify the pathogenesis of early embryo damage from the cellular and molecular perspectives,and provide theoretical basis for the diagnosis and treatment of embryo damage.Methods:From July 2018 to July 2019,30 patients with unexplained embryo damage were selected as embryo damage group,thirty normal early pregnancy patients with aspiration abortion were selected as the normal early pregnancy group.The decidual tissue and chorionic villi of the two groups were collected to observe the structural and pathological changes.Immunohistochemical method was used to detect the distribution of PCNA,Caspase-3,IL-6 and Survivin in the chorionic villi and decidual tissue,as well as the expression changes of the above four proteins.Results:Compared with the normal early pregnancy group,the chorionic villi in the early embryo damage group were obviously dysplasia,degenerative changes,structural disorders,homogeneous destruction,obvious reduction or even disappearance of trophoblast cells,inflammatory cell infiltration,more neutrophils and lymphocytes,interstitial edema and cell atrophy,accompanied by hemorrhage;The decidual tissue of the intima was not good,most of the cells were spindle-shaped,the structure was disordered,the stroma has edema,inflammatory cells can be seen,and hemorrhage existed.The results of immunohistochemistry showed that the expression of PCNA protein in chorionic villi and decidual tissue of early embryo damage group decreased significantly(P<0.05),while the expression of Caspase-3 protein increased significantly(P<0.05).The expression of IL-6 protein decreased significantly(P<0.05),and the expression of Survivin protein decreased significantly(P<0.05).Conclusion:During early pregnancy,due to the down-regulation of the expression of proliferating cell nuclear antigen PCNA and apoptosis inhibitor protein Survivin in chorionic and decidual tissues,the balance between proliferation and apoptosis is broken,which has an increase in Caspase-3 expression and a decrease in IL-6 expression.It may be that the balance of Th1/Th2 in chorionic and decidual tissues inclines to Th1,which leads to excessive apoptosis of chorionic and decidual tissues and the cessation of early embryo damage.

膜状胎盘19例临床妊娠结局分析

目的 探讨膜状胎盘发病机制及对妊娠结局的影响。方法 检索2010年4月至2021年6月甘肃省妇幼保健院产前检查并住院终止妊娠的170 621例孕妇的临床资料,选取其中产前超声诊断并经产后病理证实的19例膜状胎盘或部分性膜状胎盘病人作为研究对象。查阅病历收集19例孕妇的年龄、孕次、孕周、超声检查资料、胎儿宫内生长情况、终止妊娠的孕周、分娩方式、产前及产后出血情况及围产儿结局等资料进行回顾性分析。结果 膜状胎盘的发生率0.011 1%(19/170 621),其中既往有刮宫史12例、胎儿生长受限的16例、羊水过少15例、15例出现脐动脉血流异常、11例出现产前出血、引产经阴道分娩16例、剖宫产3例;围产儿17例死胎分娩、2例新生儿存活。结论 膜状胎盘可导致早产、流产、胎死宫内、羊水过少、胎儿宫内生长受限等严重不良围产儿结局,也可导致产前及产后大量出血,对于膜状胎盘孕妇,需要制定合理的诊疗方案,早诊断,早干预。

EPHB4与复发性流产绒毛血管生成相关性研究

目的探讨复发性流产(RSA)患者绒毛组织中EPHB4下调与绒毛血管生成障碍的关系。方法收集19例RSA患者(RSA组)和14例早孕要求终止妊娠患者(NC组)的绒毛组织样本,通过RT-qPCR和Westernblotting检测EPHB4的表达,通过免疫组化染色标记CD31计算绒毛微血管密度,并分析两者间的相关性。结果RSA组患者的组织中EPHB4的表达、微血管密度低于NC组(P<0.05)。EPHB4mRNA与绒毛微血管密度成中度正相关(r=0.4961,P=0.0362)。结论EPHB4在RSA患者中表达下调,可能通过抑制绒毛血管生成参与RSA的发生发展。

改良型十二指肠旷置术对2型糖尿病大鼠糖代谢的影响

目的探讨防反流的改良型十二指肠旷置术对2型糖尿病(type 2 diabetes mellitus,T2DM)大鼠糖代谢的影响,明确十二指肠在维持葡萄糖稳态中的作用。方法取40只5周龄的雄性SD大鼠,通过高脂饲料喂养联合低剂量链脲霉素诱导T2DM。将36只达到T2DM模型标准的大鼠随机分为3组:单纯十二指肠旷置术组(DE组)、防反流的改良型十二指肠旷置术组(MDE组)及假手术组(SO组),每组12只。术后第4周行胃肠道造影,并分别于术前及术后1、2、4、8周检测各组大鼠的体重、空腹血糖浓度、血清胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)浓度;术后8周处死大鼠,取每组大鼠胆胰袢1 cm肠段,行病理切片的苏木精-伊红(hematoxylin and eosin,HE)染色后在光学显微镜下观察肠黏膜绒毛长度。结果胃肠造影显示DE组中造影剂明显向十二指肠肠腔反流,而MDE组未见反流现象。术后1周,3组大鼠的体重较术前均短暂性显著下降(P<0.05),之后各组大鼠体重均随时间增长,组间体重无显著差异(P>0.05)。与SO组比较,MDE组和DE组术后各个时间点的空腹血糖浓度明显下降(P<0.05),GLP-1浓度显著升高(P<0.05);MDE组术后各个时间点的空腹血糖浓度均比DE组低(P<0.05),但MDE组和DE组之间术后各个时间点的血清GLP-1浓度无显著差异(P>0.05)。在肠黏膜形态学上,MDE组大鼠的胆胰袢黏膜绒毛长度均明显短于DE组和SO组(P<0.05)。结论防反流的改良型十二指肠旷置术可以有效改善T2DM大鼠的糖代谢,避免食糜向旷置的十二指肠反流,从而加强其降糖作用。

胎盘绒毛跨膜蛋白CD81、Ang与复发性流产女性早孕期复发流产的关系

目的:探究胎盘绒毛跨膜蛋白CD81、血管生成素(Ang)与复发性流产(RSA)女性早孕期复发流产的关系。方法:选择126例RSA患者为研究对象(RSA组),另选取94例正常早孕行人工流产术者为对照组。采用RT-qPCR、Western blot法检测胎盘绒毛组织CD81、Ang-1、Ang-2 mRNA及蛋白表达水平,采用电化学发光法检测血清人绒毛膜促性腺激素(HCG)水平,计算Ang-1/Ang-2 mRNA及蛋白比值,比较两组患者检测结果。结果:RSA组CD81、Ang-1、Ang-2的mRNA及蛋白表达和Ang-1/Ang-2 mRNA及蛋白表达比值均高于对照组,血清HCG水平低于对照组(P<0.05)。RSA组CD81 mRNA与Ang-1、Ang-2 mRNA呈正相关(P<0.05);RSA组CD81蛋白表达量与Ang-1、Ang-2蛋白表达量呈正相关(P<0.05),血清HCG水平与其他指标无相关性(P>0.05)。CD81、Ang-1/Ang-2的mRNA及蛋白和血清HCG均可用于预测RSA女性早孕期复发流产。结论:RSA女性早孕期复发流产与胎盘绒毛CD81、Ang-1、Ang-2 mRNA及蛋白表达量异常高表达有关,CD81、Ang-1、Ang-2可能影响胎盘血管形成而致流产。

米非司酮对人蜕膜与绒毛糖脂及雌孕激素受体的影响

本文从糖脂与激素受体等方面观察了米非司酮抗早孕的作用机理。采用Ｌａｄｉｓｃｈ微量分部、ＳｅｐｈａｄｅｘＧ－２５柱层析及高效薄层定量分析（ＨＰＴＬＣ），发现米非司酮引起蜕膜与绒毛糖酯含量及组分的变化；应用ＤＣＣ法测定蜕膜组织中雌、孕激素受体（ＥＲ、ＰＲ）。结果表明米非司酮使蜕膜组织中ＥＲ上升，ＰＲ下降，干扰了ＥＲ与ＰＲ之间的平衡。以上研究提示来非司酮抗早孕作用是多环节的，蜕膜是其重要的作用部位。

诊断级超声波辐照对人早孕绒毛组织的影响

目的 :研究早孕时经超声辐照后绒毛组织的超微结构变化和细胞化学反应变化。方法 :15例妊娠 6～ 8周健康妇女分为 4组 ,A组 (对照组 ) 3例 ,未行超声辐照 ;B组 4例 ,超声辐照 10 min;C组、D组各 4例 ,辐照 2 0 m in。B、C组超声辐照后即时取材 ,D组超声辐照后 3 d取材。观察绒毛组织超微结构和细胞化学反应的变化。结果 :C组 ,即超声辐照 2 0 m in后即时取材组 ,与正常组相比较绒毛组织有变化 ,合体滋养细胞线粒体嵴间隙及内质网扩张 ,过氧化氢细胞化学反应为阳性 ,合体滋养细胞微绒毛上有致密物沉积。A组、B组、D组均无明显异常。结论

太湖猪与大白猪小肠发育及其免疫功能形态学比较

应用组织学方法分别对 2 8头太湖猪和大白猪各发育时期小肠各段的形态学变化进行比较研究。结果表明 ,太湖猪十二指肠绒毛于 3日龄达最长 (14 7 4 9μm) ,大白猪则于 2 0日龄达到最长 (16 2 90 μm)。随着年龄的增加 ,绒毛的长度逐渐变短 ,但其宽度增加 ,肠皱襞越来越发达 ,以 90日龄和 12 0日龄最为显著。肠壁厚度随着年龄的增加而增加。太湖猪回肠的淋巴组织尽管随着生长逐渐发育 ,但不及同期大白猪。提示太湖猪与大白猪生长速度的明显差异与小肠组织发育有关 ,小肠发育是动物生长的重要因素之一。

小麦基础饲料添加木聚糖酶对尼罗罗非鱼肠道食糜粘度和绒毛、微绒毛发育的影响

以尼罗罗非鱼[体重(106.16±16.77)g]为实验对象,小麦基础饲料为对照,小麦基础饲料中分别添加不同水平的木聚糖酶(0.05%、0.10%、0.15%)作为试验饲料。每个处理设5个重复,每个重复放养40尾雄性尼罗罗非鱼。饱食投喂,饲养75d后测定尼罗罗非鱼肠道食糜粘度以及前、中、后肠的肠绒毛显微与超微结构。结果表明,对照组与0.05%、0.10%、0.15%木聚糖酶试验组的肠道食糜粘度分别为(15.21±0.29)cps、(13.75±0.45)cps、(12.23±0.16)cps和(11.92±0.22)cps,试验组的肠道食糜粘度极显著低于对照组(P<0.01)。随着木聚糖酶添加量的增加,试验组尼罗罗非鱼前、中、后肠绒毛表面的粘附性颗粒数量较对照组明显减少,颗粒直径减小;试验组前、中、后肠微绒毛的密度和高度均得到不同程度的改善。0.10%木聚糖酶试验组前、中、后肠绒毛高度、宽度、密度均极显著高于对照组(P<0.01)。

糖萜素对固始鸡小肠黏膜结构的影响

本研究旨在探讨饲粮中添加不同水平糖萜素对固始鸡小肠黏膜结构的影响。将300只固始鸡公雏随机分成5组：对照组（饲喂基础饲粮）、300mg/kg糖萜素组、500mg/kg糖萜素组、700mg/kg糖萜素组和金霉素（200mg/kg）组,每组3个重复,每个重复20只鸡。在21、35、49日龄时,分别从各组随机选取6只固始鸡,放血致死。应用常规石蜡切片,苏木精-伊红染色技术观察固始鸡十二指肠、空肠、回肠组织黏膜结构,测定其绒毛高度、隐窝深度、绒毛高度/隐窝深度（V/C）值及肠壁厚度。结果表明：1）在固始鸡的不同日龄,各组间十二指肠、空肠和回肠的长度及肠壁厚度差异均不显著（P〉0.05）。2）各日龄时,500mg/kg糖萜素组和700mg/kg糖萜素组固始鸡十二指肠绒毛高度均较高,2组间差异不显著（P〉0.05）,但21日龄时均显著高于其他组（P〈0.05）,35~49日龄时均与其他组差异不显著（P〉0.05）;各日龄时,500mg/kg糖萜素组和金霉素（200mg/kg）组固始鸡十二指肠的V/C值均较高,2组间差异不显著（P〉0.05）,但21~35日龄时均显著高于对照组（P〈0.05）,49日龄时均与其他组差异不显著（P〉0.05）。3）各日龄时,500mg/kg糖萜素组固始鸡空肠绒毛高度最高,21~35日龄时均显著高于对照组（P〈0.05）,但与700mg/kg糖萜素组和金霉素（200mg/kg）组差异不显著（P〉0.05）,49日龄时均与其他组差异不显著（P〉0.05）;各日龄时,500mg/kg糖萜素组、700mg/kg糖萜素组和金霉素（200mg/kg）组固始鸡空肠的V/C值均较高,3组间差异不显著（P〉0.05）,但均显著高于对照组（P〈0.05）。4）各日龄时,700mg/kg糖萜素组和金霉素（200mg/kg）组固始鸡回肠的绒毛高度均较高,21~35日龄时均显著高于对照组和300mg/kg糖萜素组（P〈0.05）,但与500mg/kg糖萜素组差异不显著（P〉0.05）,49日龄时均与其他组差异不显著（P〉0.05）;各日龄时,500mg/kg糖萜素组、700mg/kg糖萜素组和金霉素（200mg/kg）组固始鸡回肠的V/C值均较高,3组间差异不显著（P〉0.05）,但均显著高于对照组（P〈0.05）。结果提示,糖萜素能促进肉鸡肠绒毛生长,降低隐窝深度,从而增加肠道对营养的吸收面积,综合各方面得出500mg/kg为最适添加水平。

甘露寡糖对SPF大鼠结肠结构的影响

目的研究甘露寡糖对SPF大鼠结肠结构的影响。方法将体重100±100g、同性、同品系SPF试验大鼠分为生理盐水对照组、什露寡聚糖组、大肠菌液组、甘露寡聚糖＋乳酸菌液组共4组，每组10～20只，分开饲养。每天称小鼠体重，摄食量、水量随意。采集饲养28d的大鼠结肠，通过同定、染色，得到组织切片。结果饲喂甘露寡糖有益于结肠粘膜上皮细胞的排列，并能改善对号肠绒毛长度，肠道肌层厚度。结论饲喂不同水平的甘露寡糖对大鼠结肠组织影响较大，直接影响其组织结构。

人早孕期绒毛组织和滋养细胞趋化因子受体转录水平

目的:研究早孕期绒毛组织及滋养细胞18种趋化因子受体的转录水平,以揭示趋化因子受体在母-胎界面生理性调节作用。方法:提取人早孕期绒毛组织及滋养细胞总RNA,半定量RT-PCR检测绒毛组织和滋养细胞18种趋化因子受体mRNA的表达水平。结果:CXCR4及CXCR6在绒毛组织中普遍高表达;CCR6、CCR7、XCR1及CX3CR1呈普遍中等表达;CCR1-CCR5、CCR8-CCR10、CXCR1-CXCR3在部分绒毛组织中表达,部分绒毛组织不表达或表达量很低;早孕人绒毛组织不表达CXCR5。早孕期滋养细胞表达CCR1、CCR3-CCR5、CCR8-CCR9、CXCR1-CXCR4、CXCR6、XCR1、CX3CR1;不表达CCR2、CCR6、CCR7、CCR10及CXCR5。结论:早孕期绒毛组织及滋养细胞表达多种趋化因子受体,它们在正常妊娠中具有重要的生理学意义。

稽留流产绒毛中HIF-1α、VEGF和PLGF的表达及意义

目的:检测缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)及胎盘生长因子(PLGF)在稽留流产(MA)绒毛组织中的表达,探讨3种因子在维持正常妊娠中的作用。方法:选取昆明市第一人民医院产科收治的MA患者36例(研究组)及行人工流产的正常早孕妇女28例(对照组)。应用免疫组化法检测两组绒毛组织中HIF-1α、VEGF和PLGF的表达。结果:研究组绒毛水肿,细胞滋养细胞增生、数量增加,合体滋养细胞明显减少,甚至断裂消失。HIF-1α、VEGF、PLGF在两组妇女绒毛滋养细胞的胞浆均有表达,但研究组HIF-1α、VEGF、PLGF的阳性表达率均低于对照组(16.67 vs 57.14%,66.67%vs 100.00%,77.78%vs 100.00%,P<0.05)。结论:HIF-1a、VEGF和PLGF与正常妊娠的维持有关,其表达异常可能导致MA。