Plant communities on sandbars were important, and restoration of degraded wetland were significant to biological conservation in the East Dongting Lake region. In this study, typical wetland community types on sandbar...Plant communities on sandbars were important, and restoration of degraded wetland were significant to biological conservation in the East Dongting Lake region. In this study, typical wetland community types on sandbars and their site elevation were surveyed to explore relationship between community distribution and site elevation. Results show that eight major communities in this region were wetland communities dominated by Juncus effusus, Carex brevicuspis, Polygonum hydropiper, Salix triandra, Oenanthe Javanica, Phalaris arundinacea, Miscanthus sacchariflorus and Phragmites communis respectively. These communities had obvious constructive species and companion species, while site elevation could affect community composition and their species diversity. On sandbars in the East Dongting Lake, vegetation was largely composed of hygrophytes companied with a few mesophytes. The higher the site elevation was, the nearer the site was to the water, and the lower community diversity became. Except soil humidity and site elevation, growth period of plants after inundation could also affect species richness in the communities.展开更多
Objective In March 2012, an H7N7 subtype avian influenza virus （AIV） named A/wild goose/Dongting/PC0360/2022 （H7N7） （DT/PC0360） was recovered from a wild goose in East Dongting Lake. We performed whole-genome se...Objective In March 2012, an H7N7 subtype avian influenza virus （AIV） named A/wild goose/Dongting/PC0360/2022 （H7N7） （DT/PC0360） was recovered from a wild goose in East Dongting Lake. We performed whole-genome sequencing of the isolate, and analyzed the phylogenetic and molecular characterization. Methods RNA was extracted from environment samples （including fecal samples from wild bird or domestic ducks, and water samples） for detecting the presence of Influenza A Virus targeting Matrix gene, using realtime RT-PCR assay. The positive samples were performed virus isolation with embryonated eggs. The subtype of the isolates were identified by RT-PCR assay with the HI-HI6 and N1-N9 primer set. The whole-genome sequencing of isolates were performed. Phylogenetic and molecular characterizations of the eight genes of the isolates were analyzed. Results Our results suggested that all the eight gene segments of DT/PC0360 belonged to the Eurasian gene pool, and the HA gene were belonged to distinct sublineage with H7N9 AIV which caused outbreaks in China's Mainland in 2013. The hemagglutinin cleavage site of HA of DT/PC0360 showed characterization of low pathogenic avian influenza virus. Conclusion Strengthening the surveillance of AlVs of wild waterfowl and poultry in this region is vita for our knowledge of the ecology and mechanism of transmission to prevent an influenza pandemic.展开更多
基金supported by the National Key Technology R&D Program (2006BAD03A15)
文摘Plant communities on sandbars were important, and restoration of degraded wetland were significant to biological conservation in the East Dongting Lake region. In this study, typical wetland community types on sandbars and their site elevation were surveyed to explore relationship between community distribution and site elevation. Results show that eight major communities in this region were wetland communities dominated by Juncus effusus, Carex brevicuspis, Polygonum hydropiper, Salix triandra, Oenanthe Javanica, Phalaris arundinacea, Miscanthus sacchariflorus and Phragmites communis respectively. These communities had obvious constructive species and companion species, while site elevation could affect community composition and their species diversity. On sandbars in the East Dongting Lake, vegetation was largely composed of hygrophytes companied with a few mesophytes. The higher the site elevation was, the nearer the site was to the water, and the lower community diversity became. Except soil humidity and site elevation, growth period of plants after inundation could also affect species richness in the communities.
文摘Objective In March 2012, an H7N7 subtype avian influenza virus （AIV） named A/wild goose/Dongting/PC0360/2022 （H7N7） （DT/PC0360） was recovered from a wild goose in East Dongting Lake. We performed whole-genome sequencing of the isolate, and analyzed the phylogenetic and molecular characterization. Methods RNA was extracted from environment samples （including fecal samples from wild bird or domestic ducks, and water samples） for detecting the presence of Influenza A Virus targeting Matrix gene, using realtime RT-PCR assay. The positive samples were performed virus isolation with embryonated eggs. The subtype of the isolates were identified by RT-PCR assay with the HI-HI6 and N1-N9 primer set. The whole-genome sequencing of isolates were performed. Phylogenetic and molecular characterizations of the eight genes of the isolates were analyzed. Results Our results suggested that all the eight gene segments of DT/PC0360 belonged to the Eurasian gene pool, and the HA gene were belonged to distinct sublineage with H7N9 AIV which caused outbreaks in China's Mainland in 2013. The hemagglutinin cleavage site of HA of DT/PC0360 showed characterization of low pathogenic avian influenza virus. Conclusion Strengthening the surveillance of AlVs of wild waterfowl and poultry in this region is vita for our knowledge of the ecology and mechanism of transmission to prevent an influenza pandemic.
