期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
敲除ebony基因导致二化螟体表黑色素沉积 被引量:2
1
作者 孙浩 黄镜梅 +5 位作者 刘艳 葛文超 王帅 杨凤霞 高聪芬 吴顺凡 《昆虫学报》 CAS CSCD 北大核心 2021年第12期1367-1376,共10页
【目的】二化螟Chilo suppressalis是中国和其他亚洲地区为害水稻的重要害虫。由于缺乏遗传学操作工具,对二化螟的功能基因组学研究受到严重限制。本研究的目的是借助一个标记基因ebony,在二化螟体内建立CRISPR/Cas9介导的基因编辑体系... 【目的】二化螟Chilo suppressalis是中国和其他亚洲地区为害水稻的重要害虫。由于缺乏遗传学操作工具,对二化螟的功能基因组学研究受到严重限制。本研究的目的是借助一个标记基因ebony,在二化螟体内建立CRISPR/Cas9介导的基因编辑体系。【方法】以家蚕Bombyx mori ebony蛋白作为参考序列,通过TBLASTN程序在二化螟基因组数据库获得二化螟ebony基因的cDNA序列;通过PCR技术克隆二化螟ebony全长序列并进行生物信息学分析;运用qRT-PCR技术分析二化螟ebony基因在不同发育阶段(卵、幼虫、预蛹、蛹和雌雄成虫)和4龄幼虫不同组织(头、表皮、脂肪体、肠道和马氏管)中的表达模式;借助基于CRISPR/Cas9的基因编辑技术,把sgRNA/Cas9蛋白复合物显微注射到二化螟新鲜的卵内(产卵后2 h内),靶向敲除二化螟ebony基因。【结果】克隆得到二化螟Csebony基因(GenBank登录号:MZ846208)的全长cDNA序列,开放阅读框长为2586 bp,共编码861个氨基酸。Csebony蛋白质的分子质量为9.5 kD,理论等电点为5.10,且在N端没有信号肽。结构域分析显示,Csebony有3个保守的结构域。系统发育树显示,Csebony与玉米螟Ostrinia furnacalis的ebony亲缘关系最近。qPT-PCR结果显示Csebony基因在蛹期和头部高表达。敲除Csebony导致二化螟幼虫、蛹以及成虫黑色素沉积。【结论】本研究结果表明,Csebony参与了二化螟表皮颜色模式的调控;CRISPR/Cas9基因组编辑技术可在二化螟中有效应用。借助标记基因在非模式生物中快速建立CRISRP/Cas9基因编辑体系,可为二化螟的功能基因组学研究提供有价值的遗传学工具。 展开更多
关键词 二化螟 基因编辑 CRISPR/Cas9 ebony 标记基因 表型 脱靶效应
下载PDF
C++的快速应用开发工具Ebony
2
作者 孙岗 《电脑技术——Hello-IT》 1997年第5期22-24,共3页
关键词 C++ 应用开发工具 ebony 软件工具
下载PDF
Surrounded by Ebony
3
作者 Liu Wei 《ChinAfrica》 2011年第11期44-44,共1页
Makonde woodcarving gains an audience in China through couple Li Songshan and Han Rong THE Makonde are an ethnic group living along the Tanzania-Mozambique border.They are considered some of Africa’s most talented wo... Makonde woodcarving gains an audience in China through couple Li Songshan and Han Rong THE Makonde are an ethnic group living along the Tanzania-Mozambique border.They are considered some of Africa’s most talented woodcarvers and their craftsmanship has been passed down from generation to generation.Makonde woodcarving is often referred to as the grandfather of modern African wood sculpture.Its exaggerated human and animal patterns reflect the essence of traditional African culture,full of imagination and inspired by nature. 展开更多
关键词 Surrounded by ebony
下载PDF
CRISPR/Cas9-mediated ebony knockout results in puparium melanism in Spodoptera litura 被引量:7
4
作者 Hong-Lun Bi Jun Xu +3 位作者 Lin He Yong Zhang Kai Li Yong-Ping Huang 《Insect Science》 SCIE CAS CSCD 2019年第6期1011-1019,共9页
Insect body pigmentation and coloration are critical to adaption to the environment. To explore the mechanisms that drive pigmentation, we used the clustered regularly interspaced short palindromic repeats (CRISPR)/CR... Insect body pigmentation and coloration are critical to adaption to the environment. To explore the mechanisms that drive pigmentation, we used the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing system to target the ebony gene in the non-model insect Spodoptera litura. Ebony is crucial to melanin synthesis in insects. By directly injecting Cas9 messenger RNA and ebony-specific guide RNAs into S. litura embryos, we successfully induced a typical ebony-deficient phenotype of deep coloration of the puparium and induction of melanin formation during the pupal stage. Polymerase chain reaction-based genotype analysis demonstrated that various mutations had occurred at the sites targeted in ebony. Our study clearly demonstrates the function of ebony in the puparium coloration and also provides a potentially useful marker gene for functional studies in S. litura as well as other lepidopteran pests. 展开更多
关键词 CR1SPR/Cas9 ebony MARKER gene SPODOPTERA litura
原文传递
Characteristics of a Laboratory Strain of Coleomegilla maculata with a Novel Heritable Wing Spot Pattern Trait
5
作者 Margaret Louise Allen 《Advances in Entomology》 2016年第1期47-60,共14页
The lady beetle Coleomegilla maculata is a common New World insect that is naturally colored pink to red or orange with black spots on the forewings of the adult stage. Previous laboratory in-breeding resulted in sele... The lady beetle Coleomegilla maculata is a common New World insect that is naturally colored pink to red or orange with black spots on the forewings of the adult stage. Previous laboratory in-breeding resulted in selection for a strain lacking red pigment in the cuticle and eyes. An additional strain selected for a novel spotting pattern is described here. The inheritance of the new trait, “ten spotted” (10sp), was determined by classical crossing experiments. Inheritance of the trait was autosomal and exhibited incomplete dominance. Bionomic strain measurements were compared to the parental strains and were similar overall. Two expressed sequences from C. maculata that may be related to the new phenotype were compared to model insect genes encoding a melanin biosynthesis enzyme and a patterning transcription factor. 展开更多
关键词 COCCINELLIDAE Mutant Phenotype MELANIN ebony Wing Pattern
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部