Objective:In order to elucidate the biological activity of the Co-cultured adventitious roots(ARs) of Echinacea pallida and Echinacea purpurea and provide theoretical basis for its application,and the antiinflammatory...Objective:In order to elucidate the biological activity of the Co-cultured adventitious roots(ARs) of Echinacea pallida and Echinacea purpurea and provide theoretical basis for its application,and the antiinflammatory activities and potential mechanisms of Co-cultured ARs were studied.Methods:The experimental materials were obtained by bioreactor co-culture technology and used in the activity research.In this study,mouse macrophages induced by lipopolysaccharide(LPS) were used as in vitro model.Different concentrations of AR extract(50-400 g/mL) were used to treat cells.The expression of pro-inflammatory cytokines was determined using enzyme linked immunosorbent assay.The inducible nitric oxide synthase and cyclooxygenase-2 expression,mitogen-activated protein kinase(MAPK) phosphorylation,and the inhibitor of nuclear factor-kappa B-a levels were determined by the Western blot analysis.Results:In the co-cultured ARs,total flavonoids and total caffeic acid were determined,and the contents of both bioactive compounds were significantly higher than those ARs from the single-species culture.Compared with the control group,the large amount of pro-inflammatory mediators was released after LPS stimulation.However,in the extract groups with different concentrations(25,50,and 100 g/mL),the production of these pro-inflammatory mediators was inhibited in a dose-dependent manner.Furthermore,the levels of phosphorylation of MAPK proteins,including p-p38, p-c-Jun N-terminal kinase,and p-extracellular regulated protein kinases were significantly(P <0.05) decreased in the extract groups,revealing that the AR extract probably involved in regulating the MAPK signaling pathway.Conclusion:Collectively,our findings suggested that the co-cultured ARs of E.pallida and E.purpurea can inhibit production of pro-inflammatory mediators in mouse peritoneal macrophages and possess the anti-inflammatory effect by regulating MAPK signaling pathways.展开更多
目的:探索松果菊的耐盐能力和耐盐机理。方法:以松果菊为研究对象,设置6个盐度水平(0、30、60、90、120、150 mmol/L Na Cl),采用滤纸床发芽和盆栽耐盐法,研究了盐胁迫对松果菊种子萌发和植株生理生化等耐盐指标的影响。结果:盐胁迫抑...目的:探索松果菊的耐盐能力和耐盐机理。方法:以松果菊为研究对象,设置6个盐度水平(0、30、60、90、120、150 mmol/L Na Cl),采用滤纸床发芽和盆栽耐盐法,研究了盐胁迫对松果菊种子萌发和植株生理生化等耐盐指标的影响。结果:盐胁迫抑制了松果菊种子的萌发,诱导渗透调节物质脯氨酸、可溶性糖和K+含量的上调,以及活性氧清除物质POD和SOD活性的增加,但同时也导致了Na+的积累和K+/Na+值的减小。结论:松果菊可抵抗一定程度的盐胁迫,但当盐浓度过高时,严重的盐害作用将显著抑制松果菊的生长。展开更多
基金supported by grants the Jilin Scientific and Technological Development Program (20180101278JC) for the financial supportthe National Natural Science Foundation of China (31370388 and 31660080)。
文摘Objective:In order to elucidate the biological activity of the Co-cultured adventitious roots(ARs) of Echinacea pallida and Echinacea purpurea and provide theoretical basis for its application,and the antiinflammatory activities and potential mechanisms of Co-cultured ARs were studied.Methods:The experimental materials were obtained by bioreactor co-culture technology and used in the activity research.In this study,mouse macrophages induced by lipopolysaccharide(LPS) were used as in vitro model.Different concentrations of AR extract(50-400 g/mL) were used to treat cells.The expression of pro-inflammatory cytokines was determined using enzyme linked immunosorbent assay.The inducible nitric oxide synthase and cyclooxygenase-2 expression,mitogen-activated protein kinase(MAPK) phosphorylation,and the inhibitor of nuclear factor-kappa B-a levels were determined by the Western blot analysis.Results:In the co-cultured ARs,total flavonoids and total caffeic acid were determined,and the contents of both bioactive compounds were significantly higher than those ARs from the single-species culture.Compared with the control group,the large amount of pro-inflammatory mediators was released after LPS stimulation.However,in the extract groups with different concentrations(25,50,and 100 g/mL),the production of these pro-inflammatory mediators was inhibited in a dose-dependent manner.Furthermore,the levels of phosphorylation of MAPK proteins,including p-p38, p-c-Jun N-terminal kinase,and p-extracellular regulated protein kinases were significantly(P <0.05) decreased in the extract groups,revealing that the AR extract probably involved in regulating the MAPK signaling pathway.Conclusion:Collectively,our findings suggested that the co-cultured ARs of E.pallida and E.purpurea can inhibit production of pro-inflammatory mediators in mouse peritoneal macrophages and possess the anti-inflammatory effect by regulating MAPK signaling pathways.
文摘目的:探索松果菊的耐盐能力和耐盐机理。方法:以松果菊为研究对象,设置6个盐度水平(0、30、60、90、120、150 mmol/L Na Cl),采用滤纸床发芽和盆栽耐盐法,研究了盐胁迫对松果菊种子萌发和植株生理生化等耐盐指标的影响。结果:盐胁迫抑制了松果菊种子的萌发,诱导渗透调节物质脯氨酸、可溶性糖和K+含量的上调,以及活性氧清除物质POD和SOD活性的增加,但同时也导致了Na+的积累和K+/Na+值的减小。结论:松果菊可抵抗一定程度的盐胁迫,但当盐浓度过高时,严重的盐害作用将显著抑制松果菊的生长。
