Objective： The main objective of this study was to preliminarily determine the optimum formulation of a Chinese herbal formula that may have neuroprotective effects against rotenone-induced Parkinson＇s disease （PD...Objective： The main objective of this study was to preliminarily determine the optimum formulation of a Chinese herbal formula that may have neuroprotective effects against rotenone-induced Parkinson＇s disease （PD）. Methods： Seven recipes were made from Dihuang （DH, Rehmanniaglutinosa Libosch）, Roucongrong （RCR, Cistanche deserticola Y.C.Ma）, Niuxi （NX, Achyranthes bidentata BI.） and Shanzhuyu （SZY, Comus officinalis Sieb. et Zucc） in different proportions, according to the principles of uniform design （4 factors 7 levels）. Tyrosine hydroxylase （TH）-positive neurons in substantia nigra pars compacta （SNpc） were detected by immunohistochemistry and rotenone-exposure days necessary to induce PD symptoms were recorded. To probe one likely mechanism of the formulas, echinacoside （ECH） concentrations of all seven recipes were determined by high-performance liquid chromatography and related to number of TH-positive neu- rons. Results： The data showed that recipe 4 （DH：RCR：SZY：NX = 1：1：1：1 ） and recipe 7 （DH：RCR：SZY：NX = 7：5：3： 1） partially reversed rotenone-induced death of TH-positive neurons in the SNpc and significantly increased rotenone-e^posed days compared with model group. Pharmacologically, there was not a strong correlation between ECH concentration and TH-positive neurons. Conclusion： The investigated formulations of Chinese herbs had neuroprotective effects against PD mod- els, and the neuroprotective effects were weakly related to the proportion of key herbs. However the neu- roprotective effects of the formula may not result from a single active constituent.展开更多
基金supported by grants from Shanghai Scientific and Technical Supporting Program of China(No.12401901003)Shanghai Three-Year Action Plan to Further Accelerate the Development of Traditional Chinese Medicine(No.ZY3-CCCX-1-1015)the National Natural Science Foundation(No.NSFC81400393)
文摘Objective： The main objective of this study was to preliminarily determine the optimum formulation of a Chinese herbal formula that may have neuroprotective effects against rotenone-induced Parkinson＇s disease （PD）. Methods： Seven recipes were made from Dihuang （DH, Rehmanniaglutinosa Libosch）, Roucongrong （RCR, Cistanche deserticola Y.C.Ma）, Niuxi （NX, Achyranthes bidentata BI.） and Shanzhuyu （SZY, Comus officinalis Sieb. et Zucc） in different proportions, according to the principles of uniform design （4 factors 7 levels）. Tyrosine hydroxylase （TH）-positive neurons in substantia nigra pars compacta （SNpc） were detected by immunohistochemistry and rotenone-exposure days necessary to induce PD symptoms were recorded. To probe one likely mechanism of the formulas, echinacoside （ECH） concentrations of all seven recipes were determined by high-performance liquid chromatography and related to number of TH-positive neu- rons. Results： The data showed that recipe 4 （DH：RCR：SZY：NX = 1：1：1：1 ） and recipe 7 （DH：RCR：SZY：NX = 7：5：3： 1） partially reversed rotenone-induced death of TH-positive neurons in the SNpc and significantly increased rotenone-e^posed days compared with model group. Pharmacologically, there was not a strong correlation between ECH concentration and TH-positive neurons. Conclusion： The investigated formulations of Chinese herbs had neuroprotective effects against PD mod- els, and the neuroprotective effects were weakly related to the proportion of key herbs. However the neu- roprotective effects of the formula may not result from a single active constituent.
