The possibility of gene flow between two varieties of transgenic rice with bar gene (Y0003 and 99-t) (male) and barnyard grass(Echinochloa crusgalli var. mitis ) (female) was studied by means of reproductive biology. ...The possibility of gene flow between two varieties of transgenic rice with bar gene (Y0003 and 99-t) (male) and barnyard grass(Echinochloa crusgalli var. mitis ) (female) was studied by means of reproductive biology. The germination and growth of rice pollen grains on barnyard grass stigmas at 30 min, and 1-4 h after crossing by hand were observed with an optical microscope. The results were compared with the germination and growth of barnyard grass pollen grains at the corresponding time after self-pollination. The results showed that germination and growth of the pollen grains of the two varieties were similar on barnyard grass stigmas, but differed significantly from self-pollination of barnyard grass. Pollen grains germinated and pollen tubes penetrated stigmas normally, and the number of pollen grains being condensing or releasing their inclusions or having released them increased with the time after self-pollination. Pollen grains of transgenic rice on the stigmas of barnyard grass couldn't germinate or grow normally after crossing, neither could they penetrate the stigmas of barnyard grass. Therefore, it could be concluded that the sexual incompatibility between transgenic rice with bar gene and barnyard grass is due to the rice pollen being unable to penetrate the stigma of barnyard grass. Further proof of incompatibility lies in the fact that the emasculated barnyard grass pollinated with the rice pollen grains could not seed.展开更多
【研究目的】目前已从许多种植物中克隆了其PPDK基因,但是对于其蛋白结构的分析尚未报道,此文通过生物信息学方法对家稗PPDK的序列进行了分析,并对其三维结构进行了建模分析;【研究方法】利用DNASTAR、Vector NTI 9、SignalP等生物信息...【研究目的】目前已从许多种植物中克隆了其PPDK基因,但是对于其蛋白结构的分析尚未报道,此文通过生物信息学方法对家稗PPDK的序列进行了分析,并对其三维结构进行了建模分析;【研究方法】利用DNASTAR、Vector NTI 9、SignalP等生物信息学平台对[Echinochloa crusgalli var frumentacea(Roxb.)W.F.Wight]丙酮酸磷酸双激酶(Pyruvate orthophosphate dikinase)进行了序列分析和三维结构建模;【研究结果】家稗丙酮酸磷酸双激酶(PPDK)同来自于高等植物的PPDK具有较高的同源性,无信号肽,存在17个跨膜结构区,二级结构是由许多螺旋、转角和少量折叠构成。同时在一二级结构分析的基础上,利用同源建模的方法完成了三维结构的建模。【结论】家稗PPDK蛋白同来自高等植物中的更为相近,无信号肽,有17个跨膜结构区,其三维结构为一同源四聚体。展开更多
基金the Ministry ofScience and Technology,P.R.ChinaEvaluation of Safety of Transgenic Crops(E200102).
文摘The possibility of gene flow between two varieties of transgenic rice with bar gene (Y0003 and 99-t) (male) and barnyard grass(Echinochloa crusgalli var. mitis ) (female) was studied by means of reproductive biology. The germination and growth of rice pollen grains on barnyard grass stigmas at 30 min, and 1-4 h after crossing by hand were observed with an optical microscope. The results were compared with the germination and growth of barnyard grass pollen grains at the corresponding time after self-pollination. The results showed that germination and growth of the pollen grains of the two varieties were similar on barnyard grass stigmas, but differed significantly from self-pollination of barnyard grass. Pollen grains germinated and pollen tubes penetrated stigmas normally, and the number of pollen grains being condensing or releasing their inclusions or having released them increased with the time after self-pollination. Pollen grains of transgenic rice on the stigmas of barnyard grass couldn't germinate or grow normally after crossing, neither could they penetrate the stigmas of barnyard grass. Therefore, it could be concluded that the sexual incompatibility between transgenic rice with bar gene and barnyard grass is due to the rice pollen being unable to penetrate the stigma of barnyard grass. Further proof of incompatibility lies in the fact that the emasculated barnyard grass pollinated with the rice pollen grains could not seed.
文摘【研究目的】目前已从许多种植物中克隆了其PPDK基因,但是对于其蛋白结构的分析尚未报道,此文通过生物信息学方法对家稗PPDK的序列进行了分析,并对其三维结构进行了建模分析;【研究方法】利用DNASTAR、Vector NTI 9、SignalP等生物信息学平台对[Echinochloa crusgalli var frumentacea(Roxb.)W.F.Wight]丙酮酸磷酸双激酶(Pyruvate orthophosphate dikinase)进行了序列分析和三维结构建模;【研究结果】家稗丙酮酸磷酸双激酶(PPDK)同来自于高等植物的PPDK具有较高的同源性,无信号肽,存在17个跨膜结构区,二级结构是由许多螺旋、转角和少量折叠构成。同时在一二级结构分析的基础上,利用同源建模的方法完成了三维结构的建模。【结论】家稗PPDK蛋白同来自高等植物中的更为相近,无信号肽,有17个跨膜结构区,其三维结构为一同源四聚体。