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Expression profile of long non-coding RNAs in the intestine of black rockfish Sebastes schlegelii in response to Edwardsiella tarda infection
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作者 Xu YAN Min CAO +4 位作者 Qiang FU Ning YANG Ningning WANG Lin SONG Chao LI 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第1期376-391,共16页
Long non-coding RNAs(lncRNAs)are a class of transcripts longer than 200 bp,which have been emerged as essential regulators in numerous biological processes.Black rockfish(Sebastes schlegelii)is an economic fish that w... Long non-coding RNAs(lncRNAs)are a class of transcripts longer than 200 bp,which have been emerged as essential regulators in numerous biological processes.Black rockfish(Sebastes schlegelii)is an economic fish that widely cultured in the coastal areas of China,Japan,and South Korea.With the expansion of aquacultural scale,various pathogens have threatened its industry and reduced its economic values.It has been reported that lncRNA were involved in the immune response and metabolic pathway in teleost,while no study is available on identification and functional analysis of lncRNAs in black rockfish so far.Herein,this study was performed to identify lncRNAs in the intestine of black rockfish after Edwardsiella tarda infection.In our results,a total of 9311 lncRNAs were identified through highthroughput sequencing,and 102 lncRNAs were significantly regulated following challenge,which were predicted to target 3348 mRNAs.Results of Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses of the se target genes showed they were function in catalytic activity,hydrolase activity,defense response and peptidase activity,which involved in metabolic pathways and immune related pathways.In addition,47 lncRNAs and 8 differentially expressed mRNAs(DEmRNAs)showed co-expression at two or more infection time points with metabolism and immunity functions.Moreover,real-time quantitative PCR(qRT-PCR)was performed to verify the reliability of sequencing gene expression analysis results.This research laid the foundation for further investigation of the regulatory roles of lncRNAs in the intestinal immune response of black rockfish. 展开更多
关键词 long non-coding RNA MRNA INTESTINE Sebastes schlegelii edwardsiella tarda
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黄颡鱼(Pelteobagrus fulvidraco)“红头病”病原菌迟钝爱德华氏菌(Edwardsiella tarda)的分离及鉴定 被引量:48
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作者 邓先余 罗文 +2 位作者 谭树华 邱山红 陈康贵 《海洋与湖沼》 CAS CSCD 北大核心 2008年第5期511-516,共6页
用ATB微生物自动鉴定系统对分离自湖南湘潭地区人工养殖的患"红头病"的黄颡鱼体内的2株细菌(即HN004和HN005)进行了鉴定,发现它们的生理生化特征完全相同,均为革兰氏阴性短杆菌、接触酶阳性、吲哚阳性、H2S阳性;氧化酶阴性、... 用ATB微生物自动鉴定系统对分离自湖南湘潭地区人工养殖的患"红头病"的黄颡鱼体内的2株细菌(即HN004和HN005)进行了鉴定,发现它们的生理生化特征完全相同,均为革兰氏阴性短杆菌、接触酶阳性、吲哚阳性、H2S阳性;氧化酶阴性、V.P测定为阴性,与迟钝爱德华氏菌的表型特征非常相似。为进一步确定2株菌的分类学地位,测定了其16SrRNA基因序列,分析了相关细菌相应序列的同源性,构建分子系统发育树。结果表明,2菌株的序列完全一致,与迟钝爱德华氏菌的亲缘关系最近,相似性为99.0%。综合上述结果,2菌株可鉴定为迟钝爱德华氏菌(Edwardsiella tarda)。 展开更多
关键词 黄颡鱼 红头病 迟钝爱德华氏菌 分离 鉴定
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迟钝爱德华氏菌(Edwardsiella tarda)研究概况 被引量:31
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作者 郑大海 麦康森 《海洋湖沼通报》 CSCD 2004年第1期52-59,共8页
迟钝爱德华氏菌 (Edwardsiellatarda)是水产养殖中危害极大的病原菌 ,本文从发病情况。
关键词 迟钝爱德华氏菌 edwardsiella tarda 感染 致病性研究 防治
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Elimination of Multidrug Resistant Plasmid pEIB202 in Fish Pathogenic Edwardsiella tarda
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作者 郑浚源 许黎黎 +1 位作者 王启要 肖婧凡 《Agricultural Science & Technology》 CAS 2012年第1期227-232,共6页
[Objective] The aim of the research was to investigate the function of large plasmid pEIB202 in the pathogenesis of Edwardsiella tarda EIB202 and to eliminate the plasmid pEIB202,so as to lay the foundation for develo... [Objective] The aim of the research was to investigate the function of large plasmid pEIB202 in the pathogenesis of Edwardsiella tarda EIB202 and to eliminate the plasmid pEIB202,so as to lay the foundation for developing safe and live attenu- ated vaccine against E, tarda. [Method] sacB was used as reverse screening marker to eliminate the plasmid by using homologous recombination technique. [Result] The plasmid pEIB202 was sequenced and it was found that the plasmid encoded multiple resistant genes and some components in type IV secretion system(T4SS),which sug- gested that the plasmid might be related with the multiple drug-resistance and pathogenicity of E. tarda. The plasmid-eliminated strain EIB202Ap lost the resistance to chloramphenicol and tetracycline,but its growth,virulence and secretion of extra- cellular proteins had no significant difference with wild-type plants. [Conclusion] pEIB202 plasmid is the main reason that caused the multi-drug resistance of EIB202 and might have indirect effects in the pathogenesis of EIB202. 展开更多
关键词 edwardsiella tarda pEIB202 T4SS R plasmid VACCINE
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An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene 被引量:4
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作者 谢国驷 张庆利 +4 位作者 韩娜娜 史成银 王秀华 刘庆慧 黄倢 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第4期595-603,共9页
Edwardsiella tarda is a maj or pathogen in aquatic environments that can cause heavy economic losses. An improved method for quick and accurate detection of E. tarda by loop-mediated isothermal amplification (LAMP) ... Edwardsiella tarda is a maj or pathogen in aquatic environments that can cause heavy economic losses. An improved method for quick and accurate detection of E. tarda by loop-mediated isothermal amplification (LAMP) with two additional loop primers was developed by targeting the EsrB gene (EsrI3- LAMP). In this method, the Mg^2+ concentration, reaction temperature, and reaction time were optimized to 8 retool/L, 61℃, and 40 min, respectively. The detection limit with the EsrB gene was as low as 10 copies, which is 100 times more sensitive than that of conventional polymerase chain reaction (PCR). The EsrB-LAMP assay was shown more sensitive and rapid than previously reported LAMP assays targeting the hemolysin gene (hemolysin-LAMP) for detection ofE. tarda. The EsrB-LAMP was also highly specific to E. tarda and had no cross-reaction with 13 other strains of bacteria. The assay can be carried out in a simple heating device and the EsrB-LAMP products can be visually detected by adding fluorescent dye to the reaction mixture. Taken together, the improved EsrB-LAMP diagnostic protocol has the potential for detection ofE. tarda from indoor and outdoor samples. 展开更多
关键词 edwardsiella tarda LAMP DETECTION EsrB
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Development and validation of a TaqMan^(TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda 被引量:2
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作者 XIE Guosi HUANG Jie +3 位作者 ZHANG Qingli HAN Nana SHI Chengyin WANG Xiuhua 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2012年第4期140-148,共9页
Edwardsiella tarda is one of the most important emerging pathogens in tile global aquaculture industries. As such, an accurate diagnosis and quantitative analytical methods are urgently needed for this bacterium. In t... Edwardsiella tarda is one of the most important emerging pathogens in tile global aquaculture industries. As such, an accurate diagnosis and quantitative analytical methods are urgently needed for this bacterium. In this study, primers and a TaqMan probe specific to the conservative sequences of the 16S rRNA gene of E. tarda were designed. The concentration of primers and TaqMan probe were optimized to 200 nmol/L and 120 nmol/L, respectively. The detection sensitivity of the FQ- PCR assay was determined to be as low as five copies of the target sequence per reaction using the pGEM-16S rDNA recombinant plasmid as a template, which was 100 times more sensitive than conventional PCR. A standard curve by plotting the threshold cycle values (y) against the common logarithmic copies (logl0n~ as x; n~ is copy number) of pGEM-16S rDNA was generated. The results of intra- and inter-assay variability tests demonstrate that the established FQ-PCR method was highly reproducible. The assay was specific for E. tarda as it showed that there was no cross-reactivity to eight additional bacterial pathogen strains in aquaculture. Thus, the FQ-PCR assay has the potential for diagnostic purposes and for other applications, especially for the rapid detection and quantification of low-grade E. tarda infections. 展开更多
关键词 edwardsiella tarda TAQMAN real-time PCR detection 16S rDNA
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A real-time PCR targeted to the upstream regions of HlyB for specific detection of Edwardsiella tarda 被引量:2
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作者 谢国驷 黄倢 +4 位作者 张庆利 韩娜娜 史成银 王秀华 刘庆慧 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第5期731-737,共7页
Edwardsiella tarda has become one of the most important emerging pathogens in aquaculture industry. Therefore, a rapid, reproducible, and sensitive method for detection and quantification of this pathogen is needed ur... Edwardsiella tarda has become one of the most important emerging pathogens in aquaculture industry. Therefore, a rapid, reproducible, and sensitive method for detection and quantification of this pathogen is needed urgently. To achieve this purpose, we developed a TaqMan-based real-time PCR assay for detection and quantification orE. tarda. The assay targets the hemolysin activator HlyB domain protein of E. tarda. Our optimized TaqMan assay is capable of detecting as little as 40 fg of genomic DNA per reaction. A standard curve was generated from the threshold cycle values (y) against log10 (E. tarda genomic DNA concentration) as x. The intra- and inter-assay coefficient of variation (CV) values were less than 2.06% and 1.05% respectively, indicating that the assay had good reproducibility. This method is highly specific to E. tarda strains, as it shows no cross-reactivity to Edwardsiella ictaluri, a member of the same genus, or to nine other fish-pathogenic bacteria species belonging to three other genera. This sensitive and specific real-time PCR assay provides a valuable tool for diagnostic quantitation of E. tarda in clinical samples. 展开更多
关键词 edwardsiella tarda TAQMAN real-time PCR DETECTION
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Expressions of Toll Like Receptor(TLR)Genes in Paralichthys olivaceus After Induced by Different Extracts of Edwardsiella tarda 被引量:2
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作者 SHAN Yanan ZHENG Jinhui +1 位作者 GAO Hong SUN Jinsheng 《Journal of Ocean University of China》 SCIE CAS CSCD 2022年第4期1027-1036,共10页
Toll like receptors(TLRs)are the main innate immune‘pattern recognition receptors’of animals,which play a central role in host cell recognition and responses to invasive pathogens,particularly common structures of m... Toll like receptors(TLRs)are the main innate immune‘pattern recognition receptors’of animals,which play a central role in host cell recognition and responses to invasive pathogens,particularly common structures of microbial pathogens.In this study,the gene expression profiles of TLRs in the spleen,head kidney,gill,small intestine,liver,muscle,and heart of healthy Paralichthys olivaceus were detected by real-time quantitative PCR(qPCR).The TLR family members were widely expressed in different tissues with different basic expression profiles.The highest expressions of TLR1,5m,7,8,9,14,and 21 were found in the spleen;the highest expressions of TLR3 and TLR21 were found in the gill;the highest expressions of TLR2 and 5s were found in the small intestine.The second highest expressions of TLR3,7,and 8 were found in small intestine.The gene expression profiles of TLRs stimulated with Edwardsiella tarda DNA,RNA,and lipopolysaccharide(LPS)were also detected in spleen,head kidney and gill.TLR9 and TLR21 were sensitive to E.tarda DNA;TLR 8 and TLR21 were sensitive to E.tarda RNA;and TLR1 and TLR14 were sensitive to E.tarda LPS.The expressions of the other TLR genes showed no significant changes.The results imply that the expressions of these TLR genes in P.olivaceus are differently regulated in the whole body and play important roles in the immune response against E.tarda infection. 展开更多
关键词 edwardsiella tarda Paralichthys olivaceus TLRS EXPRESSION real-time fluorescence quantitative PCR
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Identification and Biological Characteristics of Edwardsiella tarda Isolated from Pelteobagrus fulvidraco 被引量:1
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作者 Dongren ZHOU Xueping YE +2 位作者 Yizhi LUO Weida SHI Xiaoying HANG 《Agricultural Biotechnology》 CAS 2014年第5期42-44,共3页
Over the past five years, an epidemic disease broke out in Pelteobagrus fulvidraco ponds of Zhejiang Province. The diseased fish mainly exhibits head splitting and surface bleeding; dissection results reveal hepatonep... Over the past five years, an epidemic disease broke out in Pelteobagrus fulvidraco ponds of Zhejiang Province. The diseased fish mainly exhibits head splitting and surface bleeding; dissection results reveal hepatonephromegaly and abdominal bleeding. A bacterial strain HSY201301 was isolated from the liver tissue of P. fulvidraco with typical symptoms. Artificial infection experiment confirmed that the isolated strain had a strong virulence to healthy P. fidvidraco, leading to similar symptoms to naturally infected P. fulvidraco. The isolated strain was identified as an Edwardsiella tarda strain according to conventional morphological, physiological, biochemical characteristics and 16S rRNA gene sequence. Results of drug susceptibility test indicated that the isolated strain was sensitive to cipro- floxacin, doxitard, penicillin, doxycycline, and rocephin. This study laid solid foundation for effective prevention and control of E. tarda. 展开更多
关键词 Pelteobagrus fulvidraco edwardsiella tarda 16S rRNA
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两种多糖作为迟缓爱德华氏菌(Edwardsiella tarda)灭活疫苗佐剂对大菱鲆(Scophthalmus maximus)的免疫保护效果 被引量:7
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作者 隋虎辰 谢国驷 +3 位作者 边慧慧 王秀华 张晓华 黄倢 《海洋与湖沼》 CAS CSCD 北大核心 2012年第5期1001-1007,共7页
采用黄芪多糖、葡聚糖作为免疫佐剂,与迟缓爱德华氏菌灭活疫苗配伍后注射免疫大菱鲆,测定免疫28d后血清中溶菌酶活力、SOD活力、抗体效价和各免疫组的相对保护率(RPS)。结果表明,添加多糖免疫佐剂能提高疫苗免疫的大菱鲆的各免疫指标,... 采用黄芪多糖、葡聚糖作为免疫佐剂,与迟缓爱德华氏菌灭活疫苗配伍后注射免疫大菱鲆,测定免疫28d后血清中溶菌酶活力、SOD活力、抗体效价和各免疫组的相对保护率(RPS)。结果表明,添加多糖免疫佐剂能提高疫苗免疫的大菱鲆的各免疫指标,添加佐剂的免疫组的血清溶菌酶活力、SOD活力和血清效价比单纯的疫苗免疫组显著提高(P<0.05),2.5mg/ml黄芪多糖混合疫苗免疫组和5mg/ml葡聚糖混合疫苗免疫组的相对保护率最高,分别达(78.7±1.3)%和(64.0±8.9)%,且溶菌酶活力、SOD活力及血清效价等指标较其他各组有提高。 展开更多
关键词 迟缓爱德华氏菌 大菱鲆 黄芪多糖 葡聚糖 疫苗 佐剂
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牙鲆(Paralichthys olivaceus)TLR21基因在迟缓爱德华氏菌(Edwardsiella tarda)感染后的表达特征 被引量:2
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作者 张洁 郑津辉 +5 位作者 李庆亚 耿绪云 孙金生 潘宝平 孙世南 高虹 《海洋与湖沼》 CAS CSCD 北大核心 2015年第6期1502-1508,共7页
应用荧光定量PCR技术,检测了TLR21基因在牙鲆(Paralichthys olivaceus)感染迟缓爱德华氏菌(Edwardsiella tarda)后,在0 h、1 h、3 h、6 h、12 h、1 d、3 d、6 d后,在心脏、肝脏、脾脏、头肾、鳃、小肠、肌肉和血的时空表达特征,并探讨... 应用荧光定量PCR技术,检测了TLR21基因在牙鲆(Paralichthys olivaceus)感染迟缓爱德华氏菌(Edwardsiella tarda)后,在0 h、1 h、3 h、6 h、12 h、1 d、3 d、6 d后,在心脏、肝脏、脾脏、头肾、鳃、小肠、肌肉和血的时空表达特征,并探讨了它们与牙鲆先天免疫反应的关系。结果表明,大多组织在感染病原6 h后TLR21基因表达明显上调,尤其是头肾和小肠。头肾6 h的表达量达到了对照组的59.3倍,小肠6 h的表达量为对照组的38.6倍。迟缓爱德华氏菌感染引起牙鲆体内各组织中TLR21的上调表达和变化,为研究牙鲆对迟缓爱德华氏菌的防御机制提供了理论依据。 展开更多
关键词 TLR21基因 牙鲆 迟缓爱德华氏菌 表达 实时荧光PCR
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迟缓型爱德华氏菌(Edwardsiella tarda)诱导牙鲆(Paralichthys olivaceus)TLR1及TLR2基因的表达分析 被引量:1
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作者 李庆亚 周密 +5 位作者 张洁 郑津辉 耿绪云 潘宝平 孙金生 高虹 《海洋与湖沼》 CAS CSCD 北大核心 2017年第4期848-856,共9页
Toll样受体是一类重要的蛋白质分子,参与固有免疫系统,在哺乳动物在受到细菌感染的时候,TLR1和TLR2基因可以形成异源二聚体,进而启动宿主的固有免疫。本文应用实时荧光定量PCR的技术,检测了TLR1和TLR2基因在牙鲆健康组织以及牙鲆腹腔注... Toll样受体是一类重要的蛋白质分子,参与固有免疫系统,在哺乳动物在受到细菌感染的时候,TLR1和TLR2基因可以形成异源二聚体,进而启动宿主的固有免疫。本文应用实时荧光定量PCR的技术,检测了TLR1和TLR2基因在牙鲆健康组织以及牙鲆腹腔注射迟缓型爱德华氏菌(Edwardsiella tarda)后各组织中的表达变化,并探讨了它们与牙鲆(Paralichthys olivaceus)固有免疫反应的关系。结果表明,TLR1和TLR2基因广泛表达于健康牙鲆的各种组织中,其中,TLR1在脾脏组织中表达量最高,其次是心脏、肌肉;TLR2在小肠组织中表达量最高,其次是肝脏、心脏。免疫刺激实验表明,多数组织在感染病原6h后TLR1基因表达达到峰值,其中脾脏中基因的表达量最大,是0时间点的290倍(P<0.01)。TLR2基因在感染病原1h后在脾脏中表达量最高,为0时间点的17.8倍(P<0.01),在感染病原1d后心脏组织中基因的表达量为对照组的14倍(P<0.01),其余时间点表达变化不明显。结果表明TLR1和TLR2参与了牙鲆对迟缓型爱德华氏菌的免疫应答反应。实验结果还显示,在牙鲆感染迟缓型爱德华氏菌后,MyD88、TNF-α和IL-1基因的表达也都同步上调,暗示迟缓型爱德华氏菌有可能通过TLR1通路上调MyD88的表达,并最终导致炎症因子TNF-α和IL-1的基因表达上调,以应答病原菌的感染。 展开更多
关键词 TLR1 TLR2 牙鲆 迟缓型爱德华氏菌 表达 实时荧光PCR
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抗迟缓爱德华菌(Edwardsiella tarda)单克隆抗体的制备及双抗夹心ELISA检测方法的建立
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作者 杨川 秦艺丹 +1 位作者 胡潜 李强 《生物技术通报》 CAS CSCD 北大核心 2016年第7期200-205,共6页
以迟缓爱德华菌株2CDM001为抗原,利用杂交瘤技术制备了迟缓爱德华菌的单抗8D11、8H4、2F4、18C12、16E2、20D3、19G2、5F7、4E6、7C5、18H9。在特异性方面,单抗8H4和2F4特异性较差,与美人鱼弧菌ATCC33539、鮰爱德华菌ATCC33202等参考菌... 以迟缓爱德华菌株2CDM001为抗原,利用杂交瘤技术制备了迟缓爱德华菌的单抗8D11、8H4、2F4、18C12、16E2、20D3、19G2、5F7、4E6、7C5、18H9。在特异性方面,单抗8H4和2F4特异性较差,与美人鱼弧菌ATCC33539、鮰爱德华菌ATCC33202等参考菌株均出现不同程度的交叉反应,而其余9株单抗特异性强,与实验中用到的除迟缓爱德华菌外的参考菌株均不结合;但与迟缓爱德华菌分离株的检测结果表明,仅有20D3和8H4可与所有分离株结合,其余单抗不能与实验中所有迟缓爱德华菌发生反应。由于单抗8H4与美人鱼弧菌ATCC33539有交叉反应,因此选单抗20D3和兔源多抗建立迟缓爱德华菌的双抗夹心ELISA检测方法,该方法特异性强,灵敏度达到5×10^7CFU/mL。本研究扩充了迟缓爱德华菌单克隆抗体库,为迟缓爱德华菌单克隆抗体的进一步应用提供更多参考数据和可选择的材料。 展开更多
关键词 迟缓爱德华菌 单克隆抗体 双抗夹心ELISA
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线纹海马肠道菌群结构与功能对迟钝爱德华氏菌(Edwardsiella tarda)感染的响应特征研究
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作者 张乐乐 邹强 +6 位作者 田雅楠 吕春晖 郑诗怡 姜广峻 高龙坤 侯玉平 王凯 《热带海洋学报》 CAS CSCD 北大核心 2022年第2期177-188,共12页
细菌性肠炎对海马养殖业影响巨大,但病原对海马肠道菌群的具体影响尚不清楚。文章利用已分离的病原细菌Edwardsiella tarda YT1和海马细菌性肠炎模型,结合16S rDNA高通量测序技术,探究病原细菌侵染对海马肠道菌群的影响。结果发现,E.ta... 细菌性肠炎对海马养殖业影响巨大,但病原对海马肠道菌群的具体影响尚不清楚。文章利用已分离的病原细菌Edwardsiella tarda YT1和海马细菌性肠炎模型,结合16S rDNA高通量测序技术,探究病原细菌侵染对海马肠道菌群的影响。结果发现,E.tarda侵染改变了海马肠道菌群的结构组成、多样性和丰度,并显著降低了其多样性(p<0.05);显著增加了海马肠道变形菌门(Proteobacteria)的相对丰度(p<0.05),减少了放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)的相对丰度(p<0.05);导致致病菌爱德华氏菌属(Edwardsiella)在属水平的相对丰度极显著增加(p<0.01),而肠道固有菌群嗜冷杆菌属(Psychrobacter)和罗氏菌属(Rothia)极显著减少(p<0.01),以及球菌属(Macrococcus)与动球菌属(Planococcus)显著减少(p<0.05)。研究结果表明,E.tarda能通过改变海马肠道固有优势菌群的相对丰度导致菌群失调。菌群功能变化及其相关性分析表明,E.tarda可能通过显著提高细菌趋化性、鞭毛组装、ABC转运蛋白、磷酸转运酶系统以及脂多糖生物合成途径的活性(p<0.05),抑制肠道核心菌群如嗜冷杆菌属、动球菌属和谷氨酸杆菌属的丰度及其核糖体、RNA降解、核苷酸剪切修复与脂肪酸生物合成途径的活性(p<0.05),导致肠道菌群功能失调,并诱发肠炎。 展开更多
关键词 海马 肠道菌群 肠炎 失调 迟钝爱德华氏菌
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中华鳖(Pelodiscus sinensis)迟缓爱德华氏菌(Edwardsiella tarda)的分离鉴定与药物敏感性分析 被引量:7
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作者 朱凝瑜 曹飞飞 +1 位作者 郑晓叶 郑天伦 《中国渔业质量与标准》 2018年第4期65-71,共7页
目前,细菌性疾病是中华鳖养殖中的常见疾病。实验采用常规方法从呈白底板症状的中华鳖(Pelodiscus sinensis)肝脏、脾脏、肾脏、胃积液等中分离纯化病原菌,经形态观察、生理生化鉴定和16S r RNA序列分析确定其种属,通过回归感染实验判... 目前,细菌性疾病是中华鳖养殖中的常见疾病。实验采用常规方法从呈白底板症状的中华鳖(Pelodiscus sinensis)肝脏、脾脏、肾脏、胃积液等中分离纯化病原菌,经形态观察、生理生化鉴定和16S r RNA序列分析确定其种属,通过回归感染实验判断分离菌株致病性,并以纸片扩散法测定其药物敏感性。结果显示:自病鳖中分离可得到一种优势菌落,其表面湿润光滑、微隆起、半透明,呈灰黄色;基于VITEK 2的64个生化反应的分析提示,分离菌理化特性与迟缓爱德华氏菌(Edwardsiella tarda)基本一致;16S rRNA序列分析显示,其与迟缓爱德华氏菌同源性达98%以上。腹腔注射回归感染实验引起稚鳖死亡,且细菌分离能再次得到相同分离株,表明分离到的迟缓爱德华氏菌具有致病性。药敏试验表明该菌对庆大霉素、阿米卡星、新霉素、头孢哌酮、氨曲南等5种抗生素较为敏感。本研究可为中华鳖病害的正确诊断和科学防治提供参考。 展开更多
关键词 中华鳖 白底板病 迟缓爱德华氏菌 分离鉴定 药敏实验 系统进化树 生理生化鉴定
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Antibiotics Resistance Pattern and Plasmid Profiling of <i>Edwardsiella tarda</i>Isolated from <i>Heterobranchus longifilis</i>
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作者 F. C. Ogbonne E. R. Ukazu F. C. Egbe 《Journal of Biosciences and Medicines》 2018年第4期95-105,共11页
A study was carried out to investigate antibiotic resistance patterns and plasmid profiling of Edwardsiella tarda isolated from farmed-cultured Heterobranchus longifilis in Lagos State, Southwest of Nigeria. A total o... A study was carried out to investigate antibiotic resistance patterns and plasmid profiling of Edwardsiella tarda isolated from farmed-cultured Heterobranchus longifilis in Lagos State, Southwest of Nigeria. A total of 44 Edwardsiella isolates were recovered from 80 fish samples collected from the 10 fish farms using selective random stratification. It was observed that Edwardsiella tarda isolates were 100% resistant to Amoxicillin, Chloranphenicol, Levofloxacin, Streptomycin and 90% resistant to Nalidixic Acid respectively. All the isolates were 100% susceptible to Spectinomycin and Ciprofloxacin, while Ofloxacin, Gentamycin, and Pefloxacin vary in their level of susceptibility with 90%, 80% and 70% sensitivity respectively. Conversely, 8 out of 10 fish farm locations studied were observed to have antibiotic-resistant strains, and 5 out of 8 drug-resistant strains were found to carry plasmid and the sizes of the plasmid ranges between 20.027 kb to 23.130 kb. The plasmid after treatment with mitomycin C and ethidium bromide were lost during the process of plasmid curing confirming that the multiple drug resistant exhibited by the isolates was plasmid mediated. There are fewer studies on antibiotic resistance in Edwardsiella tarda from aquaculture enterprises and this study provides further support to the view that there is a potential risk of transfer of resistant bacteria and their genes to human pathogen through the food chain. Although, in Nigeria there is no antibiotic product registered for aquaculture usage, yet fish farmers use them off-label for bacterial diseases prevention. 展开更多
关键词 edwardsiella tarda PLASMID PROFILING Antibiotic Resistance Heterobranchus longifilis
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Edwardsiella tarda and Aeromonas hydrophila isolated from diseased Southern flounder(Paralichthys lethostigma)are virulent to channel catfish and Nile tilapia
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作者 Julia W.Pridgeon Phillip H.Klesius +2 位作者 Gregory A.Lewbart Harry V.Daniels Megan Jacob 《Journal of Coastal Life Medicine》 2014年第5期337-343,共7页
Objective:To identify bacteria isolated from diseased Southern flounder and determine whether they are virulent to channel catfish and Nile tilapia.Methods:Gram-negative bacteria isolates were recovered from five tiss... Objective:To identify bacteria isolated from diseased Southern flounder and determine whether they are virulent to channel catfish and Nile tilapia.Methods:Gram-negative bacteria isolates were recovered from five tissues of diseased Southern flounder(Paralichthys lethostigma).The isolates were subjected to biochemical and molecular identification followed by virulence study in fish.Results:Based on biochemical analysis,the 25 isolates were found to share homologies with either Edwardsiella tarda(E.tarda)or Aeromonas hydrophila(A.hydrophila).Based on sequencing results of partial 16S rRNA gene,15 isolates shared 100%identities with the 16S rRNA sequence of previously identified E.tarda strain TX1,whereas the other 10 isolates shared 100%identities with the 16S rRNA sequence of previously identified A.hydrophila strain An4.When healthy fish were exposed to flounder isolate by intracoelomic injection,the LD50 values of flounder isolate E.tarda to channel catfish or Nile tilapia[(10±2)g]were 6.1×10^(4)and 1.1×10^(7)CFU/fish,respectively,whereas that of flounder isolate A.hydrophila to channel catfish and Nile tilapia were 1.4×10^(7)and 5.6×10^(7)CFU/fish,respectively.Conclusions:This is the first report that E.tarda and A.hydrophila isolated from diseased Southern flounder are virulent to catfish and tilapia. 展开更多
关键词 edwardsiella tarda Aeromonas hydrophila Southern flounder PATHOGEN VIRULENCE
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Estimation of heritabilities of disease resistance to Edwardsiella tarda and genetic correlations between resistance and growth traits in Chinese tongue sole (Cynoglossus semilaevis)
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作者 Ming Li Yingming Yang +3 位作者 Weiwei Zheng Zhongming Li Jiayu Cheng Yangzhen Li 《Aquaculture and Fisheries》 2020年第6期289-293,共5页
Chinese tongue sole(Cynoglossus semilaevis)is an important marine flatfish which is widely cultured in coastal areas in China.Infectious bacterial diseases such as Edwardsiella tarda imposed serious threats to this sp... Chinese tongue sole(Cynoglossus semilaevis)is an important marine flatfish which is widely cultured in coastal areas in China.Infectious bacterial diseases such as Edwardsiella tarda imposed serious threats to this species.A potential strategy to prevent this disease is to select resistance strains.The aim of the present work was to estimate the genetic variation of disease resistance to E.tarda and to evaluate the genetic correlations between resistance traits and growth traits.Three types of models were fitted by using different trait definitions(binary,continuous and categorical).After a 9-day challenge test,the overall survival was 75.4%(ranging from 6.4%to 100%in families),and at test day 6,the overall survival was 50.7%.We set test day 6 and 9 as cut-off point times respectively.The heritabilities of survival traits were ranging from 0.10 to 0.36.Considerably higher heritability values were obtained at day 6 than at day 9,regardless of which model or trait definition used(except Trait 2 in LIN).The genetic correlations between disease resistance traits and growth traits(i.e.body weight and total length)were low and not significant from zero(−0.12–0.24).There is a substantial re-ranking of families when defined resistance as categorical and continuous traits compared to binary trait.These results confirm the existence of genetic variation for resistance against E.tarda and weak genetic correlations indicate that joint genetic improvement of E.tarda resistance and growth is scarcely available. 展开更多
关键词 HERITABILITY Genetic parameter Disease resistance Genetic correlation edwardsiella tarda Cynoglossus semilaevis
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Prevalence of Edwardsiella tarda in commercially important finfish and shellfish of Bihar and West Bengal,India
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作者 Pankaj Kumar Harresh Adikesavalu Thangapalam Jawahar Abraham 《Journal of Coastal Life Medicine》 2016年第1期30-35,共6页
Objective:To study the prevalence of Edwardsiella tarda(E.tarda)in finfish and shellfish of West Bengal and Bihar,India and their antibiogram.Methods:Fish samples were enriched overnight in Edwardsiella ictaluri broth... Objective:To study the prevalence of Edwardsiella tarda(E.tarda)in finfish and shellfish of West Bengal and Bihar,India and their antibiogram.Methods:Fish samples were enriched overnight in Edwardsiella ictaluri broth and plated onto Edwardsiella ictaluri agar.Typical colonies were identified conventionally and by VITEK 2 compact system.Antibiogram was done by agar disc diffusion assay.Results:Of the 118 fish samples screened,only 14.41%had E.tarda.The incidence was marginally high in wild fish(15.30%)than in cultured fish(13.26%).Maximum incidence was in intestine(12.98%)followed by gills(6.60%)and skin(2.38%).All E.tarda strains were sensitive to ciprofloxacin and exhibited varying degrees of resistance to other antibiotics.Multiple antibiotic resistance was seen in 84.00%–87.50%of the E.tarda strains.Majority of them had high minimal inhibitory concentration values(200μg/mL)for oxytetracycline and gentamycin.Conclusions:The results suggested that considerable proportions of commercially important finfish are carriers of multiple antibiotic resistance E.tarda.This calls for proper sanitary measures to eliminate this pathogen in fish and fishery products. 展开更多
关键词 Fish market edwardsiella tarda Incidence rate Antibiotic resistance Minimal inhibitory concentration
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迟缓爱德华氏菌(Edwardsiella tarda)产生的二聚和三聚吲哚类生物碱 被引量:1
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作者 杨春丽 韩茵 +2 位作者 王乂 张晓华 朱伟明 《微生物学通报》 CAS CSCD 北大核心 2010年第9期1325-1330,共6页
为了开发利用病原菌资源,研究了迟缓爱德华氏菌(Edwardsiella tarda)的次生代谢产物,采用凝胶柱色谱、反相高效液相色谱等分离纯化手段,从该菌发酵产物的乙酸乙酯提取物中分离得到5个吲哚类生物碱1-5。结合其理化性质和波谱学特征,化合... 为了开发利用病原菌资源,研究了迟缓爱德华氏菌(Edwardsiella tarda)的次生代谢产物,采用凝胶柱色谱、反相高效液相色谱等分离纯化手段,从该菌发酵产物的乙酸乙酯提取物中分离得到5个吲哚类生物碱1-5。结合其理化性质和波谱学特征,化合物1-5的结构依次鉴定为:bis(1H-indol-3-yl)phenylmethane(1)、1,1-bis(1H-indol-3-yl)-2-phenylethane(2)、(2S)-3,3-bis(1H-indol-3-yl)propane-1,2-diol(3)、1H,1′H,1′′H-3,2′:3′,3′′-terindole(4)和(3,2′:2′,3′′-terindo-lin)-3′-one(5),其中化合物1、2和4为新天然产物。利用药敏纸片法、二倍稀释法和SRB法,对5个单体化合物的抗菌和细胞毒活性进行了初步评价。其中化合物1和3对产气杆菌有微弱的抑制作用,最小抑菌浓度(MIC)均为0.125g/L。 展开更多
关键词 迟缓爱德华氏菌 二聚和三聚吲哚类生物碱 抗细菌活性
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