Early filiform needle acupuncture for poststroke depression: a meta-analysis of 17 randomized controlled clinical trials

OBJECTIVE： To evaluate the effectiveness and safety of filiform needle acupuncture for poststroke depression, and to compare acupuncture with the therapeutic efficacy of antidepressant drugs. DATA RETRIEVAL： We retrieved data from the Chinese National Knowledge Infrastructure （1979-2012）, Wanfang （1980-2012）, VIP （1989-2012）, Chinese Biomedical Literature （1975- 2012）, PubMed （1966-2012）, Ovid Lww （-2012）, and Cochrane Library （-2012） Database using the internet. SELECTION CRITERIA： Randomized controlled trials on filiform needle acupuncture versus antidepressant drugs for treatment of poststroke depression were included. Moreover, the in- cluded articles scored at least 4 points on the Jadad scale. Exclusion criteria： other acupuncture therapies as treatment group, not stroke-induced depression patients, score 〈 4 points, non-ran- domized controlled trials, or animal trials. MAIN OUTCOME MEASURES： These were the Hamilton Depression Scale scores, clinical ef- fective rate, Self-Rating Depression Scale scores, Side Effect Rating Scale scores, and incidence of adverse reaction and events. RESULTS： A total of 17 randomized controlled clinical trials were included. Meta-analysis results displayed that after 4 weeks of treatment, clinical effective rate was better in patients treated with fill- form needle acupuncture than those treated with simple antidepressant drugs [relative risk = 1.11, 95% confidence interval （C/）： 1.03-1.21, P = 0.01]. At 6 weeks, clinical effective rate was similar between filiform needle acupuncture and antidepressant drug groups. At 2 weeks after filiform needle acupuncture, Hamilton Depression Scale （17 items） scores were lower than in the antide- pressant drug group （mean difference = -2.34, 95%CI： -3.46 to -1.22, P 〈 0.000,1）. At 4 weeks, Hamilton Depression Scale （24 items） scores were similar between filiform needle acupuncture and antidepressant drug groups. Self-Rating Depression Scale scores were lower in filiform needle acupuncture group than in the antidepressant drug group. Side Effect Rating Scale was used in only two articles, and no meta-analysis was conducted. Safety evaluation of the 17 arti- cles showed that gastrointestinal tract reactions such as nausea and vomiting were very common in the antidepressant drug group. Incidence of adverse reaction and events was very low in the filiform needle acupuncture group. CONCLUSION： Early filiform needle acupuncture for poststroke depression can perfectly con- trol depression. Filiform needle acupuncture is safe and reliable. Therapeutic effects of filiform needle acupuncture were better than those of antidepressant drugs.

Radiofrequency ablation combined with transcatheter arterial chemoembolization for recurrent liver cancer

BACKGROUND The recurrence rate of liver cancer after surgery is high.Radiofrequency ablation(RFA)combined with transcatheter arterial chemoembolization(TACE)is an effective treatment for liver cancer;however,its efficacy in recurrent liver cancer remains unclear.AIM To investigate the clinical effect of TACE combined with RFA in the treatment of recurrent liver cancer.METHODS Ninety patients with recurrent liver cancer were divided into 2 groups according to treatment plan:Control(RFA alone);and experimental[TACE combined with RFA(TACE+RFA)].The incidence of increased alanine aminotransferase levels,complications,and other indices were compared between the two groups before and after the procedures.RESULTS One month after the procedures,the short-term efficacy rate and Karnofsky Performance Status scores of the experimental group were significantly higher than those of the control group(P<0.05).Alpha-fetoprotein(AFP)and total bilirubin levels were lower than those in the control group(P<0.05);The overall response rate was 82.22%and 66.67%in the experimental and control groups,respectively;The disease control rate was 93.33%and 82.22%in the experimental and control groups,respectively,the differences are statistically significant(P<0.05).And there were no statistical differences in complications between the two groups(P>0.05).CONCLUSION TACE+RFA was effective for the treatment of recurrent liver cancer and significantly reduced AFP levels and improved various indices of liver function.

Application of fiberoptic bronchscopy in patients with acute exacerbations of chronic obstructive pulmonary disease during sequential weaning of invasive-noninvasive mechanical ventilation

BACKGROUND:Early withdrawal of invasive mechanical ventilation(IMV) followed by noninvasive MV(NIMV) is a new strategy for changing modes of treatment in patients with acute exacerbations of chronic obstructive pulmonary disease(AECOPD) with acute respiratory failure(ARF).Using pulmonary infection control window(PIC window) as the switch point for transferring from invasive to noninvasive MV,the time for early extubation can be more accurately judged,and therapy efficacy can be improved.This study aimed to prospectively investigate the clinical effectiveness of fiberoptic bronchscopy(FOB) in patients with AECOPD during sequential weaning of invasive-noninvasive MV.METHODS:Since July 2006 to January 2011,106 AECOPD patients with ARF were treated with comprehensive medication and IMV after hospitalization.Patients were randomly divided into two groups according to whether fiberoptic bronchoscope is used(group A,n=54) or not(group B,n=52) during sequential weaning from invasive to noninvasive MV.In group A,for sputum suction and bronchoalveolar lavage(BAL),a fiberoptic bronchoscope was put into the airway from the outside of an endotracheal tube,which was accompanied with uninterrupted use of a ventilator.After achieving PIC window,patients of both groups changed to NIMV mode,and weaned from ventilation.The following listed indices were used to compare between the groups after treatment:1) the occurrence time of PIC,the duration of MV,the length of ICU stay,the success rate of weaning from MV for the first time,the rate of reventilatJon and the occurrence rate of ventilator-associated pneumonia(VAP);2) the convenience and safety of FOB manipulation.The results were compared using Student's f test and the Chi-square test.RESULTS:The occurrence time of PIC was(5.01 ±1.49) d,(5.87±1.87) d in groups A and B,respectively(P<0.05);the duration of MV was(6.98±1.84) d,(8.69±2.41) d in groups A and B,respectively(P<0.01);the length of ICU stay was(9.25±1.84) d,(11.10±2.63) d in groups A and B,respectively(P<0.01);the success rate of weaning for the first time was 96.30%,76.92%in groups A and B,respectively(P<0.01);the rate of reventilation was 5.56%,19.23%in groups A and B,respectively(P<0.05);and the occurrence rate of VAP was 3.70%,23.07%in groups A and B,respectively(P<0.01).Moreover,it was easy and safe to manipulate FOB,and no side effect was observed.CONCLUSIONS:The application of FOB in patients with AECOPD during sequential weaning of invasive-noninvasive MV is effective in ICU.It can decrease the duration of MV and the length of ICU stay,increase the success rate from weaning MV for the first time,reduce the rate of reventilation and the occurrence rate of VAP.In addition,such a method is convenient and safe in patients of this kind.

Effects of herbal medicine in gastroesophageal reflux disease symptoms: a systematic review and meta-analysis

Background: Pyrosis and regurgitation are the cardinal symptoms of gastroesophageal reflux disease. Severalherbs have been used for treating gastrointestinal disorders worldwide. This systematic review was conducted toinvestigate the effects of medicinal herbs on gastroesophageal reflux disease and adverse events. Methods:MEDLINE (via PubMed;The United States National Library of Medicine, USA), Scopus, ScienceDirect,Cochrane Central Register of Controlled Trials, Web of Science, Magiran, and Scientific Information Databasewere systematically searched for human studies, without a time frame, using medical subject heading terms such as“gastroesophageal reflux disease”, “reflux”, “esophagitis” and “herbs”. Manual searches completed the electronicsearches. Results: Thirteen randomized controlled trials were identified, including 1,164 participants from 1,509publications. In comparing herbal medicine to placebo, there were no significant differences in terms of heartburn(P = 0.23 and 0.48), epigastric or abdominal pain (P = 0.35), reflux syndrome (P = 0.12), and effective rate (P =0.60), but there was a significant difference in terms of acid regurgitation (P = 0.01). In comparing herbal medicineto drugs, there was a significant difference in terms of effective rate (P = 0.001), and there was one trial thatreported a significant difference in terms of epigastric pain (P = 0.00001). Also, in comparing herbal medicine todrugs, there were no significant differences in terms of acid regurgitation (P = 0.39). Conclusion: Thismeta-analysis showed that herbal medicines are effective in treating gastroesophageal reflux disease. Furtherstandardized researches with a large-scale, multicenter, and rigorous design are needed.

基于网状Meta分析对8个经典名方治疗不稳定型心绞痛的临床疗效评价

目的:运用网状Meta分析方法系统评价8种经典名方联合西医常规治疗不稳定型心绞痛(UAP)的临床疗效,并分别进一步比较不同经典名方治疗实证和虚证(包括虚实夹杂)2大证型的疗效。方法:检索并筛选中文科技期刊数据库(CCD)、中国生物医学文献数据库(CBM)、中国国家知识基础设施数据库(CNKI)与中国学术期刊数据库(CSPD)等数据库中关于8个经典名方(血府逐瘀汤、补阳还五汤、四妙勇安汤、丹参饮、炙甘草汤、枳实薤白桂枝汤、瓜蒌薤白半夏汤、化痰祛瘀通脉汤)联合西医常规治疗UAP的随机对照试验(RCT),检索时限从各数据库建库至2022年1月14日,采用Stata 16.0软件进行网状Meta分析。结果:共纳入60个RCT,总计5088例UAP患者,涉及9种干预措施。其中,UAP属实证的有41个RCT,UAP患者共3432例,涉及5种干预措施;虚证或虚实夹杂共13个,共1130例患者,涉及4种干预措施。网状Meta分析结果提示,枳实薤白桂枝汤+西医常规治疗总有效率最优,UAP属实证者,四妙勇安汤+西医常规治疗最佳;虚证或虚实夹杂者,补阳还五汤+西医常规治疗最佳;心电图改善方面,四妙勇安汤疗效最佳;枳实薤白桂枝汤+西医常规在降低超敏C反应蛋白(hs-CRP)改善炎症反应方面更优。结论:与西医常规治疗UAP比较,枳实薤白桂枝汤联合西药常规治疗疗效较显著。但仍需要纳入更多高质量的RCT证实。

2022-2023年度湖南省油菜病虫害防控植保贡献率评价

为科学评估病虫害对油菜生产的影响程度和植保措施减损增产的重要作用,湖南省植保植检站在祁东、鼎城、桃江、永兴组织开展2022—2023年度油菜病虫害防控植保贡献率评价工作。通过设置严格防控、统防统治、农户自防和完全不防治病虫(对照)处理,采用大区试验,评估不同试验县(区)不同油菜病虫害防控处理的植保贡献率。结果表明:各试点处理的实际损失率为0.17%~67.56%,病虫害防控处理的植保贡献率为4.10%~67.56%。调查统计结果显示,参试县(区)油菜病虫害防控(不含杂草)植保贡献率分别为祁东25.73%、鼎城35.49%、桃江23.50%、永兴33.36%;湖南省油菜病虫防控植保贡献率为28.51%,据此测算,2022—2023年度全省通过病虫害防控约挽回菜籽损失7.38亿kg。

2023年邹城市春花生植保贡献率的测算评估

为了客观反映植保工作在花生生产中对病虫草害的控制效果,评价植保工作在花生生产中的作用,2023年选取全国花生绿色防控示范县邹城为测试点,开展花生重大病虫害植保贡献率的评价。试验设严格综防区、农户自防区和空白对照区,通过试验示范区的防控效果、花生产量、投入成本三个指标进行综合评估。结果表明:2023年邹城市试验区花生病虫草害的防控植保贡献率为33.1%,严格综防区较农户自防区增收11642.85元/hm^(2),表明农户自防区还有很大的增收空间。本项工作通过科学防控可实现增产增效目的,为其他地区春花生植保贡献率的评估提供了技术参考。

湖北省2023年度小麦病虫害防控植保贡献率初步评估

为了科学评估植保技术措施对防灾减灾的重要作用,2023年度湖北省植物保护总站在荆州区、仙桃市、宜城市、随县、安陆市等地开展了小麦病虫害防控植保贡献率评价试验。通过采用多点试验测产,结果表明:2023年湖北省小麦病虫害防控成效显著,严格科学防控区、统防统治区、农民自防区的植保贡献率分别为31.48%、25.79%、18.98%,其中严格科学防控区比农民自防区的植保贡献率高12.5百分点,统防统治区比农民自防区的植保贡献率高6.81百分点。如果在完全不防治的情况下,全省小麦病虫害造成的损失率将达31.48%。选择5个试验县的结果,经科学评估,湖北小麦病虫害防控植保贡献率为22.56%;根据湖北小麦实际总产量,以此测算,2023年湖北省小麦病虫害防控挽回粮食损失10.21亿kg。

门冬胰岛素治疗2型糖尿病患者对不良反应发生率的影响

目的探究门冬胰岛素治疗2型糖尿病患者对不良反应发生率的影响。方法选取2020年1月—2023年1月江阴市第三人民医院收治的60例2型糖尿病患者作为研究对象,并随机分为对照组和观察组,每组30例。其中,对照组采用二甲双胍治疗,观察组采用门冬胰岛素治疗,比较两组的血糖水平(FBG、2hPG、HbAlc)、不良反应发生率(胃肠道反应、乳酸性酸中毒、局部皮肤过敏)及治疗3个月后病情控制有效率(显效、有效、无效)。结果治疗前,两组血糖水平差异不显著(P>0.05);治疗后,观察组空腹血糖、餐后2h血糖及糖化血红蛋白均低于对照组(P<0.05)。观察组的不良反应总发生率显著低于对照组(P<0.05)。治疗3个月后,观察组的病情控制显效高于对照组,无效低于对照组,总有效率显著高于对照组(P<0.05)。结论门冬胰岛素可以显著降低2型糖尿病患者的不良反应发生率。

中医药治疗慢性肾脏病临床效果的meta分析

目的采用系统评价与meta分析探讨中医药治疗慢性肾脏病的临床效果。方法计算机检索国内外数据库,并按照纳入和排除标准筛选出符合条件的文献,经文献数据提取、质量评价后采用RevMan5.3软件进行meta分析。结果纳入17篇随机对照试验(RCT),共1151例患者,大多数纳入试验质量偏低。meta分析结果显示,试验组的总有效率(I2=33%,RR=1.31,95%CI:1.22~1.40)、肾小球滤过率(I2=50%,WMD=4.04,95%CI:2.04~6.05)高于对照组,血肌酐(I2=29%,WMD=-15.42,95%CI:-18.32~-12.51)、尿素氮(I2=77%,WMD=-1.27,95%CI:-1.81~-0.73)、中医证候积分(I2=89%,WMD=-6.97,95%CI:-9.47~-4.47)、24 h尿蛋白定量(I2=0%,WMD=-0.12,95%CI:-0.21~-0.03)低于对照组,差异有统计学意义(P<0.05)。结论中医药联合西医常规治疗慢性肾脏病,效果更佳,可改善中医症状和肾功能。

蒲城县小麦与玉米病虫害防控植保贡献率测算试验

为科学评估蒲城县小麦、玉米生产中植物保护工作的贡献而设计试验,设置严格防治区、统防统治区、农户自防区和空白对照区,采用对角线5点取样调查的方法,在小麦返青期杂草化学除草后和灌浆期调查病虫草害发生情况,在玉米整个生育期调查病虫草害发生情况;在小麦、玉米成熟期进行理论测产和实际测产,测算不同防治水平下的亩产量、挽回损失、植保贡献率等,根据取样代表面积在全县种植面积占比计算县域植保贡献率。结果表明,植保工作可有效控制小麦、玉米病虫草害的发生,通过防治小麦、玉米病虫害发生种类和程度均明显减轻;通过计算得出蒲城县小麦生产中植保贡献率为20.56%;玉米生产中植保贡献率为23.5%。

油菜病虫害防控效果暨植保贡献率评价试验初报--以祁东县为例

本文通过祁东县油菜病虫草害绿色防控植保贡献率评价试验,显示了重、中、轻三个不同发生区的严格综防区油菜虫害和草害防效均达85%~95%,油菜菌核病防效达88.87%~90.55%,年度重、中、轻三个不同发生区平均植保贡献率分别为45.96%、27.95%、25.68%;严格综防、统防统治和农民自防植保贡献率分别为41.98%、34.8%、18.17%,全县加权平均植保贡献率为25.73%。

阴道用乳酸杆菌活菌胶囊辅助治疗念珠菌性阴道炎的应用效果分析

目的探讨念珠菌性阴道炎患者经阴道用乳酸杆菌活菌胶囊辅助治疗后获得的临床效果。方法方便选取2020年2月—2022年4月临沂市河东医院68例念珠菌性阴道炎患者为研究对象。依据投掷硬币法分为参照组(n=34)选择特比萘芬药物治疗,研究组(n=34)在参照组基础上,选择阴道用乳酸杆菌活菌胶囊治疗。比较两组患者用药总有效率、临床病症评分(外阴灼痛感、外阴瘙痒)以及疾病复发率。结果研究组用药总有效率97.06%高于参照组用药总有效率76.47%,差异有统计学意义(χ^(2)=6.275,P<0.05);治疗后,研究组念珠菌性阴道炎患者外阴灼痛感、外阴瘙痒评分高于参照组,差异有统计学意义(P均<0.05);研究组念珠菌性阴道炎患者疾病复发率低于参照组,差异有统计学意义(P<0.05)。结论临床对念珠菌性阴道炎患者在特比萘芬药物治疗基础上,采用阴道用乳酸杆菌活菌胶囊辅助治疗,可显著提升疾病治疗效果,有效改善临床病症,降低疾病复发率,促进念珠菌性阴道炎患者良好预后。

2022年全国玉米病虫害防控植保贡献率评价报告

为客观反映粮食作物病虫害防控的成效和贡献率,2022年全国农业技术推广服务中心组织河北、吉林、河南和云南4省植保体系开展玉米重大病虫防控植保贡献率评价工作.通过统一试验方案,设置严格综防区、统防统治区、农民自防区和完全不防区,采用多点测产,经科学评估,2022年全国玉米病虫害(不包括草害和鼠害)防控植保贡献率为18.74%.统计分析表明,严格防控情况下,植保贡献率比农户自防高8.44%;统防统治条件下,植保贡献率也比农户自防高4.26%.玉米“虫口夺粮”仍有较大潜力可挖.

点阵激光联合复方倍他米松注射液治疗斑秃的临床效果分析

目的分析点阵激光联合复方倍他米松注射液治疗斑秃的临床效果。方法选取内蒙古科技大学包头医学院第一附属医院皮肤科门诊接收的60例斑秃患者,随机分为参照组和观察组,各30例。参照组患者给予复方倍他米松注射液导入治疗,观察组患者给予点阵激光联合复方倍他米松注射液导入治疗。比较两组治疗前和治疗后的毛发镜检查情况、斑秃面积、生活质量、血液流变学、免疫学指标、甲襞微循环、治疗总有效率、疾病复发率以及不良反应发生率。结果治疗后,观察组患者毛囊密度(89.51±3.82)个/cm^(2)和毛发密度(123.42±3.99)根/cm^(2)高于参照组,毳毛率(10.29±1.67)%低于参照组(P<0.05)。观察组的斑秃面积(2.75±0.27)cm^(2)少于参照组,生活质量评分(3.79±2.82)分低于参照组(P<0.05)。观察组患者全血黏度高切、全血黏度低切水平低于参照组,TNF-α和TGF-β1也低于参照组(P<0.05)。观察组患者模糊管襻、畸形管襻、粒线流发生率均低于参照组(P<0.05)。观察组的治疗总有效率为96.77%,高于参照组的73.33%(P<0.05)。观察组的疾病复发率为3.33%,低于参照组的26.67%(P<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论斑秃患者给予点阵激光联合复方倍他米松注射液治疗,可优化毛发镜检查情况,维持患者较好生活质量,提高治疗总有效率,且不良反应发生较少。

增强型体外反搏治疗老年稳定性冠心病的临床效果及对心脏重塑、神经内分泌因子和心率减速力的影响

目的探讨增强型体外反搏(EECP)治疗老年稳定性冠心病(SCAD)患者的临床效果及对心脏重塑、神经内分泌因子和心率减速力(DC)的影响。方法选取2019年5月—2022年5月至成都市第三人民医院心内二科就诊的老年SCAD患者110例,根据信封法随机分成2组:增强型体外反搏(EECP)组(n=55)和对照组(n=55)。对照组予常规药物治疗,EECP组在对照组基础上增加EECP治疗。比较2组治疗前后心室重构指标、神经内分泌因子水平、DC及治疗后临床疗效、不良反应。结果研究期间脱落3例,EECP组完成55例,对照组完成52例,最终共107例患者纳入本研究。治疗后,EECP组左室收缩末期内径(LVESD)和容积(LVESV)、左室舒张末期内径(LVEDD)和容积(LVEDV)数值及PTH、AngⅡ、ALD、PRA水平均明显低于对照组(P<0.05);EECP组DC、临床疗效显著高于对照组(P<0.05);2组患者不良反应发生率差异无统计学意义(P>0.05)。结论使用EECP治疗老年SCAD,疗效显著,可有效延缓心室重构,改善神经内分泌因子水平及DC,具有一定的应用价值。

拔毒生肌散治疗慢性难愈性创面的系统评价和Meta分析

目的:探究拔毒生肌散治疗慢性难愈性创面的临床疗效。方法:计算机检索国家知识基础设施数据库(CNKI)、中国学术期刊数据库(CSPD)、中文科技期刊数据库(CCD)、中国生物医学文献数据库(CBM)、美国医学文献数据库(Medicine)及考克兰图书馆数据库(the Cochrane Library),并通过搜查关键词检索筛选相关文献,搜集拔毒生肌散治疗慢性难愈性创面疗效的临床随机对照试验(RCT),检索时限均从建库至2022年10月。由2名评价者分别按照纳排标准筛选文献、提取资料并评价纳入研究的偏倚风险后,采用RevMan5.3软件进行系统评价和Meta分析。结果:共纳入14篇RCT文献,包括787例患者,其中观察组394例,对照组393例。Meta分析结果显示:观察组创面的愈合率高于对照组(OR=4.08,95%CI为2.55~6.55,P<0.00001);观察组创面的有效率高于对照组(OR=6.08,95%CI为3.45~10.73,P<0.00001);观察组创面的愈合时间低于对照组(MD=-3.07,95%CI为-4.12~-2.03,P<0.00001);观察组的疼痛评分低于对照组(MD=-1.18,95%CI为-2.03~-0.33,P=0.006)。结论:临床应用拔毒生肌散可以提高慢性难愈性创面的愈合率和有效率,并且缩短创面的愈合时间,减轻疼痛。但因纳入研究的局限性,上述结论仍然需要更多高质量研究验证。

7.2亿CFU/g嘧泰多抗复合微生物菌剂对烟草靶斑病和野火病的防效研究

为研究新生防制剂7.2亿CFU/g嘧泰多抗复合微生物菌剂对烟草靶斑病和野火病的防效,开展了不同药剂室内抑菌试验和田间药效试验。结果表明,7.2亿CFU/g嘧泰多抗复合微生物菌剂300倍液对烟草靶斑病病原菌和野火病病原菌的室内抑菌率均在60%以上;第3次药后,7.2亿CFU/g嘧泰多抗复合微生物菌剂300倍液对烟草靶斑病和野火病的田间防效均在65%以上,持效期长。7.2亿CFU/g嘧泰多抗复合微生物菌剂可作为兼防烟草靶斑病和野火病的推荐生防药剂。

万安县水稻病虫害综合防控植保贡献率

【目的】水稻作为最重要的粮食作物,水稻螟虫、稻纵卷叶螟、稻飞虱、稻瘟病和纹枯病对水稻产量影响显著,为评估水稻病虫害防控的植保贡献率提高作物产量。【方法】从万安县3个乡镇区域选择代表性样地,分别以水稻病虫害完全不防治为对照,开展了水稻病虫害科学防治区、统防统治区、农户自防区、虫害不防治区和病害不防区6种处理控制试验,以分析水稻病虫害防控的植保贡献率。【结果】5种措施的植保防控贡献率分别为38.60%、28.97%、27.16%、17.84%、22.73%。结合2022年万安县域水稻田间病虫常年轻发生、中等发生和重发生统计面积与区域,植保贡献率分别为19.96%、21.59%和26.01%,县域水稻病虫害防控总植保贡献率为22.19%。【结论】研究结果对评估区域水稻病虫害绿色防控和农药科学管理具有理论意义和实践指导价值。

贝莱斯芽孢杆菌ZHX-7的分离鉴定及抑菌促生效果

为获得高效拮抗花生病害的生防微生物,以花生冠腐病菌为指示菌,采用稀释涂布平板法和平板对峙法,从山东莱西采集的花生连作田的病、健株根际土壤样品中筛选到1株高效生防菌株,编号为ZHX-7。结合形态学特征和16S rDNA、gyrB序列分析,确定ZHX-7为贝莱斯芽孢杆菌(Bacillus velezensis)。ZHX-7的菌悬液、无菌发酵液和挥发性气体对花生白绢病菌、基腐病菌、网斑病菌和轮斑病菌菌丝生长的抑制均达到极显著的水平,平板对峙试验表明ZHX-7的菌悬液对花生白绢病菌、基腐病菌、网斑病菌和轮斑病菌的抑菌率分别为64.40%、70.37%、77.43%和65.32%。在以ZHX-7无菌发酵液稀释10倍的PDA培养基上,花生白绢病菌、基腐病菌、网斑病菌和轮斑病菌菌丝的生长抑制率分别为67.81%、52.16%、23.52%和69.93%。双皿对扣试验表明ZHX-7的挥发性气体对花生白绢病菌、基腐病菌、网斑病菌和轮斑病菌的抑菌率分别为86.82%、35.40%、81.70%和44.19%。ZHX-7可产生蛋白酶、纤维素酶和淀粉酶等次级代谢产物;此外,在温室盆栽条件下,ZHX-7有菌发酵液明显促进了花生的生长,同对照相比,鲜质量、干质量分别增加31.24%和26.47%,并且ZHX-7有菌发酵液明显提高了花生对白绢病的抗性,防效达到51.76%。综上所述,本研究筛选获得的一株贝莱斯芽孢杆菌ZHX-7,能够抑制多种花生病原真菌,同时促进花生生长,是一株具有宽广拮抗谱的多功能菌株,具有较好的生防应用潜力。