Strong evidence has accumulated to show a correlation between depression symptoms and inflammatory responses.Moreover,anti-inflammatory treatment has shown partial effectiveness in alleviating depression symptoms.Lyci...Strong evidence has accumulated to show a correlation between depression symptoms and inflammatory responses.Moreover,anti-inflammatory treatment has shown partial effectiveness in alleviating depression symptoms.Lycium barbarum polysaccharide(LBP),derived from Goji berries,exhibits notable antioxidative and anti-inflammatory properties.In our recent double-blinded randomized placebo-controlled trial,we found that LBP significantly reduced depressive symptoms in adolescents with subthreshold depression.It is presumed that the antidepressant effect of LBP may be associated with its influence on inflammatory cytokines.In the double-blinded randomized controlled trial,we enrolled 29 adolescents with subthreshold depression and randomly divided them into an LBP group and a placebo group.In the LBP group,adolescents were given 300 mg/d LBP.A 6-week follow up was completed by 24 adolescents,comprising 14 adolescents from the LBP group(15.36±2.06 years,3 men and 11 women)and 10 adolescents from the placebo group(14.9±1.6 years,2 men and 8 women).Our results showed that after 6 weeks of treatment,the interleukin-17A level in the LBP group was lower than that in the placebo group.Network analysis showed that LBP reduced the correlations and connectivity between inflammatory factors,which were associated with the improvement in depressive symptoms.These findings suggest that 6-week administration of LBP suppresses the immune response by reducing interleukin-17A level,thereby exerting an antidepressant effect.展开更多
Objective:To assess the best suitable condition for virus inactivation.and to study the immunogenic potential and protective efficacy of a circulating West Nile virus(WNV) strain in Assam.Methods:Bulk preparation of c...Objective:To assess the best suitable condition for virus inactivation.and to study the immunogenic potential and protective efficacy of a circulating West Nile virus(WNV) strain in Assam.Methods:Bulk preparation of circulating WNV:WNIRGC07(GeneBank ID:HQ246154).was undertaken in a bioreaclor using eytodex-1.Virus Inactivation was done in three different conditions:22 ℃.4 ℃ and room temperature.The virus preparations were evaluated for antigenicity by ELISA and toxicity by cell proliferation kit.Virus efficacy was done in-viro on swiss albino mice against standard Indian WNV and Japanese encephalitis virus(JKV)strain.Humoral and cell mediated immune response was evaluated in mice sera by ELISA and neutralization assay.Results:Inactivation at 22 ℃ was found to be more suitable in terms of less toxicity and high antigenicity.The same was selected to study the immune response and efficacy in mice.It induced neutralizing antibody titre of 1:625 and high EgG response.In vivo experiment showed 100% protective efficacy against WNV and 20.8% cross protective efficacy against JEV.Further assessment of cellular immunity through immunized mice revealed augmentation of high levels of pro-inflammatory cytokines and moderate levels of anti—cytokines indicating a mixed balance of Th1 and Th2 response.Conclusions:Findings suggest that formalin inactivated Indian WNV strain has a good immunogenic potential.This is the first study on assessment of immunogenic potential of a lineage 5 strain of WNV.Our study reveals that it would be a promising and effective candidate for vaccine studies which warrants further evaluation.展开更多
目的:探讨痰热清用于治疗胸外科术后患者的临床疗效,及其对血清TNF-α、IL-6、IL-8等炎性细胞因子的影响。方法:选择本院胸外科2014年2月至2015年12月诊治的90例胸外科术后(肺癌术后48例,食管癌术后42例)的患者作为研究对象,采用随机数...目的:探讨痰热清用于治疗胸外科术后患者的临床疗效,及其对血清TNF-α、IL-6、IL-8等炎性细胞因子的影响。方法:选择本院胸外科2014年2月至2015年12月诊治的90例胸外科术后(肺癌术后48例,食管癌术后42例)的患者作为研究对象,采用随机数字表法随机分为2组,每组各45例。对照组给予西医常规的抗感染方案行抗感染治疗。观察组则在对照组的基础上加用痰热清注射液治疗,于术后即给予痰热清20 m L静脉滴注治疗,连续治疗7 d。观察2组的临床疗效。同时采用ELISA方法检测2组患者血清中治疗前及治疗后TNF-α、IL-6、IL-8含量的变化。结果:经治疗后,观察组的临床疗效优于对照组,差异有统计学意义(P<0.05);2组患者治疗后白细胞、中性粒细胞的含量均有所下降,差异均具有统计学意义(P<0.05);白细胞、中性粒细胞下降明显低于对照组,差异均具有统计学意义(P<0.05);观察组气道分泌物有效清除率、肺部感染发生率等优于对照组,差异均具有统计学意义(P<0.05);2组患者血清中治疗后TNF-α、IL-6、IL-8的含量均有所下降,差异均具有统计学意义(P<0.05);而观察组的下降程度较对照组更为明显,差异均具有统计学意义(P<0.05)。结论:胸外科术后即使用痰热清注射液联合抗生素抗感染治疗更能有效地改善胸外科术后患者的临床症状,并能降低其血清中TNF-α、IL-6、IL-8等炎性细胞因子的含量,说明痰热清注射液发挥抑制机体炎性反应,改善临床症状,可能与之通过抑制组织中TNF-α、IL-6、IL-8等炎性反应因子的生成或释放进入血清,降低血清中炎性细胞因子的含量有关,但其具体机制仍需后期进一步深入探讨。展开更多
基金supported by the National Natural Science Foundation of China,No.81671347(to KL)the Science and Technology Program of Guangzhou of China,No.202007030012(to KFS and KL)the Science and Technology Program of Guangzhou of China,No 202102020735(to RW).
文摘Strong evidence has accumulated to show a correlation between depression symptoms and inflammatory responses.Moreover,anti-inflammatory treatment has shown partial effectiveness in alleviating depression symptoms.Lycium barbarum polysaccharide(LBP),derived from Goji berries,exhibits notable antioxidative and anti-inflammatory properties.In our recent double-blinded randomized placebo-controlled trial,we found that LBP significantly reduced depressive symptoms in adolescents with subthreshold depression.It is presumed that the antidepressant effect of LBP may be associated with its influence on inflammatory cytokines.In the double-blinded randomized controlled trial,we enrolled 29 adolescents with subthreshold depression and randomly divided them into an LBP group and a placebo group.In the LBP group,adolescents were given 300 mg/d LBP.A 6-week follow up was completed by 24 adolescents,comprising 14 adolescents from the LBP group(15.36±2.06 years,3 men and 11 women)and 10 adolescents from the placebo group(14.9±1.6 years,2 men and 8 women).Our results showed that after 6 weeks of treatment,the interleukin-17A level in the LBP group was lower than that in the placebo group.Network analysis showed that LBP reduced the correlations and connectivity between inflammatory factors,which were associated with the improvement in depressive symptoms.These findings suggest that 6-week administration of LBP suppresses the immune response by reducing interleukin-17A level,thereby exerting an antidepressant effect.
文摘Objective:To assess the best suitable condition for virus inactivation.and to study the immunogenic potential and protective efficacy of a circulating West Nile virus(WNV) strain in Assam.Methods:Bulk preparation of circulating WNV:WNIRGC07(GeneBank ID:HQ246154).was undertaken in a bioreaclor using eytodex-1.Virus Inactivation was done in three different conditions:22 ℃.4 ℃ and room temperature.The virus preparations were evaluated for antigenicity by ELISA and toxicity by cell proliferation kit.Virus efficacy was done in-viro on swiss albino mice against standard Indian WNV and Japanese encephalitis virus(JKV)strain.Humoral and cell mediated immune response was evaluated in mice sera by ELISA and neutralization assay.Results:Inactivation at 22 ℃ was found to be more suitable in terms of less toxicity and high antigenicity.The same was selected to study the immune response and efficacy in mice.It induced neutralizing antibody titre of 1:625 and high EgG response.In vivo experiment showed 100% protective efficacy against WNV and 20.8% cross protective efficacy against JEV.Further assessment of cellular immunity through immunized mice revealed augmentation of high levels of pro-inflammatory cytokines and moderate levels of anti—cytokines indicating a mixed balance of Th1 and Th2 response.Conclusions:Findings suggest that formalin inactivated Indian WNV strain has a good immunogenic potential.This is the first study on assessment of immunogenic potential of a lineage 5 strain of WNV.Our study reveals that it would be a promising and effective candidate for vaccine studies which warrants further evaluation.
文摘目的:探讨痰热清用于治疗胸外科术后患者的临床疗效,及其对血清TNF-α、IL-6、IL-8等炎性细胞因子的影响。方法:选择本院胸外科2014年2月至2015年12月诊治的90例胸外科术后(肺癌术后48例,食管癌术后42例)的患者作为研究对象,采用随机数字表法随机分为2组,每组各45例。对照组给予西医常规的抗感染方案行抗感染治疗。观察组则在对照组的基础上加用痰热清注射液治疗,于术后即给予痰热清20 m L静脉滴注治疗,连续治疗7 d。观察2组的临床疗效。同时采用ELISA方法检测2组患者血清中治疗前及治疗后TNF-α、IL-6、IL-8含量的变化。结果:经治疗后,观察组的临床疗效优于对照组,差异有统计学意义(P<0.05);2组患者治疗后白细胞、中性粒细胞的含量均有所下降,差异均具有统计学意义(P<0.05);白细胞、中性粒细胞下降明显低于对照组,差异均具有统计学意义(P<0.05);观察组气道分泌物有效清除率、肺部感染发生率等优于对照组,差异均具有统计学意义(P<0.05);2组患者血清中治疗后TNF-α、IL-6、IL-8的含量均有所下降,差异均具有统计学意义(P<0.05);而观察组的下降程度较对照组更为明显,差异均具有统计学意义(P<0.05)。结论:胸外科术后即使用痰热清注射液联合抗生素抗感染治疗更能有效地改善胸外科术后患者的临床症状,并能降低其血清中TNF-α、IL-6、IL-8等炎性细胞因子的含量,说明痰热清注射液发挥抑制机体炎性反应,改善临床症状,可能与之通过抑制组织中TNF-α、IL-6、IL-8等炎性反应因子的生成或释放进入血清,降低血清中炎性细胞因子的含量有关,但其具体机制仍需后期进一步深入探讨。
