As an aging-associated degenerative disease,Alzheimer’s disease is characterized by the deposition of amyloid beta(Aβ),oxidative stress,inflammation,dysfunction and loss of cholinergic neurons.Colla Corii Asini(CCA)...As an aging-associated degenerative disease,Alzheimer’s disease is characterized by the deposition of amyloid beta(Aβ),oxidative stress,inflammation,dysfunction and loss of cholinergic neurons.Colla Corii Asini(CCA)is a traditional Chinese medicine which has been used for feebleness-related diseases and anti-aging.CCA might delay aging-induced degenerative changes in neurons.In the present study,we evaluated antioxidant activity,cytoprotective effects,and Aβremovability of enzyme-digested Colla Corii Asini(CCAD).Oxygen radical absorbance capacity(ORAC)activity assay showed that,as compared to gelatins from the skin of porcine,bovine and cold water fish,CCA exhibited the highest ORAC activity.The ORAC activity of CCA and CCAD was increased gradually by the length of time in storage.Ultrastructure analysis by scanning electron microscopy showed that among CCA manufactured in 2008,2013,2017 and gelatin from cold water fish skin,CCA manufactured in 2008 presented the smoothest surface structure.We further tested the protective effects of CCAD(manufactured in 2008)and enzyme-digested gelatin from cold water fish skin(FGD)on hydrogen peroxide(H2O2)-induced cell death in nerve growth factor-differentiated neuronal-like PC12 cells.Presto blue assay showed that both FGD and CCAD at 0.5 mg/m L increased cell viability in H2O2-treated neuronal-like PC12 cells.The protection of CCAD was significantly superior to that of FGD.Acetylcholinesterase(Ach E)assay showed that both FGD and CCAD inhibited Ach E activity in nerve growth factor-differentiated neuronal-like PC12 cells to 89.1%and 74.5%of that in non-treated cells,respectively.The data suggest that CCAD might be able to increase the neurotransmitter acetylcholine.Although CCAD inhibited Ach E activity in neuronal-like PC12 cells,CCAD prevented H2O2-induced abnormal deterioration of Ach E.ELISA and neprilysin activity assay results indicated that CCAD reduced amyloid beta accumulation and increased neprilysin activity in Aβ1–42-treated neuronal-like PC12 cells,suggesting that CCAD can enhance Aβclearance.Our results suggest that CCA might be useful for preventing and treating Alzheimer’s disease.展开更多
E-jiao(Colla Corii Asini,CCA)has been widely used as a healthy food and Chinese medicine.Although authentic CCA is characterized by its typical sweet and neutral fragrance,its aroma components have been rarely investi...E-jiao(Colla Corii Asini,CCA)has been widely used as a healthy food and Chinese medicine.Although authentic CCA is characterized by its typical sweet and neutral fragrance,its aroma components have been rarely investigated.This work investigated the aroma-active components and antioxidant activity of 19 CCAs from different geographical origins.CCA extracts obtained by simultaneous distillation and extraction were analyzed by gas chromatography-mass spectrometry(GC-MS),gas chromatography-olfactometry(GC-O)and sensory analysis.The antioxidant activity of CCAs was determined by ABTS and DPPH assays.A total of 65 volatile compounds were identified and quantified by GC-MS and 23 aroma-active compounds were identified by GC-O and aroma extract dilution analysis.The most powerful aroma-active compounds were identified based on the flavor dilution factor and their contents were compared among the 19 CCAs.Principal component analysis of the 23 aroma-active components showed 3 significant clusters.Canonical correlation analysis between antioxidant assays and the 23 aroma-active compounds indicates strong correlation(r=0.9776,p=0.0281).Analysis of aroma-active components shows potential for quality evaluation and discrimination of CCAs from different geographical origins.展开更多
Objective:To identify Ejiao and its adulterants at the DNA level by using DNA molecula marker.Ejiao(Asini Corii Colla)is a commonly used medicinal material.However,its adulteration is a serious concern.Due to the morp...Objective:To identify Ejiao and its adulterants at the DNA level by using DNA molecula marker.Ejiao(Asini Corii Colla)is a commonly used medicinal material.However,its adulteration is a serious concern.Due to the morphological characteristics of Asini Corii Colla and its adulterants,traditional identification techniques often complex and professional,which is not conducive to the circulation management and safety of the medicinal materials.To improve the distinction between Asini Corii Colla and its adulterants accurately,this study identified and its adulterant samples based on the CytB sequence.Sequence characteristics,Basic Local Alignment Search Tool(BLAST)application,genetic distance,construction of phylogenetic tree showed the CytB sequence to accurately identify Asini Corii Colla from its adulterants.Furthermore,in this study,we designed a specific primer,based on the CytB sequence,and established a PCR detection system for rapid,sensitive,and specific identification of Asini Corii Colla.Compared to DNA barcoding technology,this method has shorter detection time,stronger specificity,and higher sensitivity,which lays the foundation for the rapid identification of Asini Corii Colla.展开更多
Objective:As an important food therapy product with traditional Chinese medicine(TCM) applications,donkey-hide gelatin(Asini Corii Colla,ACC) has been used for thousands of years.However,till now few effective strateg...Objective:As an important food therapy product with traditional Chinese medicine(TCM) applications,donkey-hide gelatin(Asini Corii Colla,ACC) has been used for thousands of years.However,till now few effective strategy had been proposed to distinguish ACC from other animal hide gelatins,especially closely related horse-and mule-hide gelatins,which was an embarrassment of ACC quality control.Methods:Combined mass spectrometry and bioinformatic methods have been applied to identify and verify two ACC-specific peptides(Pep-1 and Pep-2) capable of distinguishing ACC from other closely related animal gelatins with high selectivity.Results:It confirmed that these two peptides could be not only used for distinguishing ACC from highly homologous horse-hide and mule-hide gelatins as well as other animal hide gelatins.Conclusion:The present study provides a simple method for species-specific peptides discovery,which can be used for assessing the quality of animal gelatin products,and ensure they are authenticable and traceable.展开更多
基金supported in part by the Japan Society for the Promotion of Science(JSPS)Grant-in-Aid for Scientific Research,No.17K11813(to LX)Tai Shan Industrial Experts Program of China No.TSCY20170233(to FL)。
文摘As an aging-associated degenerative disease,Alzheimer’s disease is characterized by the deposition of amyloid beta(Aβ),oxidative stress,inflammation,dysfunction and loss of cholinergic neurons.Colla Corii Asini(CCA)is a traditional Chinese medicine which has been used for feebleness-related diseases and anti-aging.CCA might delay aging-induced degenerative changes in neurons.In the present study,we evaluated antioxidant activity,cytoprotective effects,and Aβremovability of enzyme-digested Colla Corii Asini(CCAD).Oxygen radical absorbance capacity(ORAC)activity assay showed that,as compared to gelatins from the skin of porcine,bovine and cold water fish,CCA exhibited the highest ORAC activity.The ORAC activity of CCA and CCAD was increased gradually by the length of time in storage.Ultrastructure analysis by scanning electron microscopy showed that among CCA manufactured in 2008,2013,2017 and gelatin from cold water fish skin,CCA manufactured in 2008 presented the smoothest surface structure.We further tested the protective effects of CCAD(manufactured in 2008)and enzyme-digested gelatin from cold water fish skin(FGD)on hydrogen peroxide(H2O2)-induced cell death in nerve growth factor-differentiated neuronal-like PC12 cells.Presto blue assay showed that both FGD and CCAD at 0.5 mg/m L increased cell viability in H2O2-treated neuronal-like PC12 cells.The protection of CCAD was significantly superior to that of FGD.Acetylcholinesterase(Ach E)assay showed that both FGD and CCAD inhibited Ach E activity in nerve growth factor-differentiated neuronal-like PC12 cells to 89.1%and 74.5%of that in non-treated cells,respectively.The data suggest that CCAD might be able to increase the neurotransmitter acetylcholine.Although CCAD inhibited Ach E activity in neuronal-like PC12 cells,CCAD prevented H2O2-induced abnormal deterioration of Ach E.ELISA and neprilysin activity assay results indicated that CCAD reduced amyloid beta accumulation and increased neprilysin activity in Aβ1–42-treated neuronal-like PC12 cells,suggesting that CCAD can enhance Aβclearance.Our results suggest that CCA might be useful for preventing and treating Alzheimer’s disease.
基金Authors are grateful to the financial support from the National Natural Science Foundation of China(Grants Nos.21706233,21776321,21576297,21665022,21476270)Lu Xu is financially supported by Tongren Culture,Science and Technology Industry Innovation Research Center and the China Postdoctoral Science Project(No.2016M602719)+3 种基金Guizhou Engineering Research Center(QJHKYZ[2017]024)Students Innovation and Entrepreneurship Training Center of Food Science and Technology in Tongren University(No.2016SJDCZX001)the Open Research Program(Nos.2015ZD001,2015ZD002 and 2015ZY006)the Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei province(South-Central University for Nationalities).
文摘E-jiao(Colla Corii Asini,CCA)has been widely used as a healthy food and Chinese medicine.Although authentic CCA is characterized by its typical sweet and neutral fragrance,its aroma components have been rarely investigated.This work investigated the aroma-active components and antioxidant activity of 19 CCAs from different geographical origins.CCA extracts obtained by simultaneous distillation and extraction were analyzed by gas chromatography-mass spectrometry(GC-MS),gas chromatography-olfactometry(GC-O)and sensory analysis.The antioxidant activity of CCAs was determined by ABTS and DPPH assays.A total of 65 volatile compounds were identified and quantified by GC-MS and 23 aroma-active compounds were identified by GC-O and aroma extract dilution analysis.The most powerful aroma-active compounds were identified based on the flavor dilution factor and their contents were compared among the 19 CCAs.Principal component analysis of the 23 aroma-active components showed 3 significant clusters.Canonical correlation analysis between antioxidant assays and the 23 aroma-active compounds indicates strong correlation(r=0.9776,p=0.0281).Analysis of aroma-active components shows potential for quality evaluation and discrimination of CCAs from different geographical origins.
基金supported by Scientific Research Fund Projects of Hubei Food and Drug Administration in 2016-2018 under Grant No.20160212.
文摘Objective:To identify Ejiao and its adulterants at the DNA level by using DNA molecula marker.Ejiao(Asini Corii Colla)is a commonly used medicinal material.However,its adulteration is a serious concern.Due to the morphological characteristics of Asini Corii Colla and its adulterants,traditional identification techniques often complex and professional,which is not conducive to the circulation management and safety of the medicinal materials.To improve the distinction between Asini Corii Colla and its adulterants accurately,this study identified and its adulterant samples based on the CytB sequence.Sequence characteristics,Basic Local Alignment Search Tool(BLAST)application,genetic distance,construction of phylogenetic tree showed the CytB sequence to accurately identify Asini Corii Colla from its adulterants.Furthermore,in this study,we designed a specific primer,based on the CytB sequence,and established a PCR detection system for rapid,sensitive,and specific identification of Asini Corii Colla.Compared to DNA barcoding technology,this method has shorter detection time,stronger specificity,and higher sensitivity,which lays the foundation for the rapid identification of Asini Corii Colla.
基金funded by the National Natural Science Foundation of China (No.81973450)the National Key R&D Program of China (No.2018YFC1706100)+4 种基金the Jiangsu Qinglan Projectthe Jiangsu “333” Projectthe Young Researchers Training Project of China Association of Traditional Chinese Medicine (No.QNRC2C14)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No.19KJB360020)the Open Project of Chinese Materia Medica First-Class Discipline of Nanjing University of Chinese Medicine (2020YLXK009)。
文摘Objective:As an important food therapy product with traditional Chinese medicine(TCM) applications,donkey-hide gelatin(Asini Corii Colla,ACC) has been used for thousands of years.However,till now few effective strategy had been proposed to distinguish ACC from other animal hide gelatins,especially closely related horse-and mule-hide gelatins,which was an embarrassment of ACC quality control.Methods:Combined mass spectrometry and bioinformatic methods have been applied to identify and verify two ACC-specific peptides(Pep-1 and Pep-2) capable of distinguishing ACC from other closely related animal gelatins with high selectivity.Results:It confirmed that these two peptides could be not only used for distinguishing ACC from highly homologous horse-hide and mule-hide gelatins as well as other animal hide gelatins.Conclusion:The present study provides a simple method for species-specific peptides discovery,which can be used for assessing the quality of animal gelatin products,and ensure they are authenticable and traceable.
