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Proliferation of Microorganisms in Acidic Fermentation of Elaeis guineensis L. Waste
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作者 M. Adedolapo Orimoloye A. Isaac Sanusi 《Advances in Microbiology》 2016年第9期644-649,共6页
An investigation into the type of fermentation oil palm fruit waste undergoes and the probable microorganisms involved within a short period was carried out using simple fermenter. The temperature was determined using... An investigation into the type of fermentation oil palm fruit waste undergoes and the probable microorganisms involved within a short period was carried out using simple fermenter. The temperature was determined using mercury thermometer, the pH of the medium was monitored with calibrated pH meter and the titratable acidic was determined using standard technique. The microbial profile of the medium was also evaluated using standard procedures. The highest temperature value was observed at day 0 (32.65&deg;C) and the lowest at day 1 (29.50&deg;C). The pH values of the fermentation oil palm fruit waste ranged between 4.15 - 4.60. The highest pH value was obtained at day 3 of the fermentation which was 4.60. The titratable acidity showed variation from day 0 - 2 and then with a continuous decrease till day 5. The least titratable acidity was obtained at day 5 (0.03) and the highest at day 2 (0.77). Bacteria load decreases from 1.0 × 10<sup>8</sup> - 1.6 × 10<sup>7 </sup>cfu/ml, while the fungi population increases from day 0 to day 5 of the fermentation period (1 × 10<sup>3</sup> - 2 × 10<sup>4 </sup>sfu/ml). Bacterial isolates obtained were Micrococcus leteus, Proteus vulgaris, Bacillus cereus, Baccillus subtilis, and Staphylococcus aureus while the fungal isolates obtained were Aspergillus niger, Neurospora crassa, Brachysporium spp, and Saccharomyces cerevisiae. It can be concluded that oil palm waste fermentation is an acidic fermentation that involved mesophiles microbes. And with these, several tons of oil palm epicarp waste can be optimally fermented (though with further research) and used for other purposes thereby reducing environmental pollution that would have resulted leaving this oil palm fruit waste in the environment. 展开更多
关键词 elaeis guineensis L. FERMENTATION MICROORGANISM PROLIFERATION WASTE
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Assessment of Genetic Diversity of NIFOR Oil Palm Main Breeding Parent Genotypes Using Microsatellite Markers
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作者 Maxwell N. Okoye Michael I. Uguru +2 位作者 Claude Bakoumé Rajinder Singh Christy O. Okwuagwu 《American Journal of Plant Sciences》 2016年第1期218-237,共20页
The genetic diversity among 15 NIFOR breeding parents was assessed using 10 microsatellite markers. A high genetic diversity was observed with a total of 64 alleles including 23 rare alleles or alleles at frequencies ... The genetic diversity among 15 NIFOR breeding parents was assessed using 10 microsatellite markers. A high genetic diversity was observed with a total of 64 alleles including 23 rare alleles or alleles at frequencies less than 0.05. The NIFOR tenera parents recorded the highest number of rare alleles. The average observed heterozygosity and mean gene diversity across all parental groups were 0.6889 and 0.7029, respectively. Higher genetic diversity was detected among the NIFOR dura and tenera parents compared to that of the Deli dura parents in absolute terms. Analysis of molecular variance (AMOVA) showed that 87% of the total variation (p < 0.001) observed was due to differences among parents. Rogers’ genetic distance ranged from 0.2988 to 0.8000 (mean = 0.5570). The dendrogram constructed on the basis of Rogers’ genetic distance clustered the parents in three groups. They generally clustered in heterotic manner rather than by geographic origins. The groupings obtained through PCoA confirmed the results obtained by cluster analysis. The results obtained are strong assets for NIFOR breeding programme. 展开更多
关键词 Allelic Diversity elaeis guineensis Jacq Genetic Distance HETEROSIS Molecular Characterization NIFOR Population Genetics Selection SSR Markers
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不同油棕品种对干旱胁迫及复水的生理响应及抗旱性评价 被引量:2
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作者 周丽霞 杨蒙迪 赵志浩 《分子植物育种》 CAS 北大核心 2023年第12期4066-4077,共12页
本研究以9个油棕品种盆栽一年生苗为试验材料,研究干旱胁迫对各品种油棕叶片中丙二醛(MDA)、游离脯氨酸(Pro)、可溶性糖(SS)、可溶性蛋白(SP)含量、相对电导率,以及超氧化物歧化酶(SOD)和过氧化物酶(POD)活性等生理指标的影响,并采用主... 本研究以9个油棕品种盆栽一年生苗为试验材料,研究干旱胁迫对各品种油棕叶片中丙二醛(MDA)、游离脯氨酸(Pro)、可溶性糖(SS)、可溶性蛋白(SP)含量、相对电导率,以及超氧化物歧化酶(SOD)和过氧化物酶(POD)活性等生理指标的影响,并采用主成分分析综合评价不同油棕品种的抗旱性。结果表明,随着低温胁迫时间的延长,9个品种的丙二醛(MDA)、游离脯氨酸(Pro)、可溶性糖(SS)含量和相对电导率逐渐上升;可溶性蛋白(SP)含量逐渐下降;SOD和POD的活性先升高后下降。通过主成分分析发现游离脯氨酸(Pro)含量、相对电导率、可溶性糖(SS)含量和SOD活性可作为油棕抗寒能力评价的生理指标,9个油棕品种的抗寒性由强到弱为:RY9>NG1>RY8>CR4>CR6>CR2>CR7>CR3>CR5。即油棕品种RY9、NG1和RY8的抗旱性相对较好,CR7、CR3和CR5的抗旱性相对较差。 展开更多
关键词 油棕(elaeis guineensis) 干旱胁迫 复水 生理指标 抗旱性评价
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油棕果肉发育过程中的代谢组学和转录组学联合分析 被引量:1
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作者 韦露 司成成 +3 位作者 冯美利 张安妮 张淑岩 曹红星 《分子植物育种》 CAS 北大核心 2023年第19期6315-6326,共12页
油棕是世界上生产效率最高的油料作物,果肉中含有丰富的脂肪酸。研究油棕果肉的脂质代谢物的动态变化规律及重要脂肪酸代谢相关调控基因有利于改善棕榈油品质和提高油棕果肉含油量。选用不同发育时期的油棕果肉[授粉后95 d(MT1),授粉后1... 油棕是世界上生产效率最高的油料作物,果肉中含有丰富的脂肪酸。研究油棕果肉的脂质代谢物的动态变化规律及重要脂肪酸代谢相关调控基因有利于改善棕榈油品质和提高油棕果肉含油量。选用不同发育时期的油棕果肉[授粉后95 d(MT1),授粉后125 d(MT2),授粉后185 d(MT3)]为研究对象,结合液相色谱串联质谱技术的代谢组学和高通量测序技术的转录组学,分析果肉发育过程中脂肪酸代谢物含量的动态变化规律,并根据果肉不同发育时期的差异表达基因寻找出脂质代谢物生物合成与累积的关联酶基因,结果表明,油棕果肉发育过程中游离肉豆蔻酸的含量分别从44.33、225.33 nmol/g增加到371.80 nmol/g,硬脂酸含量从451.25、1922.86 nmol/g增加到2988.75 nmol/g,棕榈油酸含量从25.22、80.37 nmol/g增加到118.02 nmol/g,亚油酸含量从1586.44、6680.46 nmol/g增加到10188.00 nmol/g。这4种游离脂肪酸的含量变化可作为判别MT1、MT2和MT3的依据;乙酰辅酶A羧化酶(ACC)、生物素羧化酶(BC)、脂烯酰基-酰基载体蛋白还原酶Ⅰ(FabⅠ)、3-氧酰基-酰基载体蛋白还原酶(FabG)、3-氧酰基-酰基载体蛋白合成酶Ⅰ(FabB)和丙二酰-辅酶A-酰基载体蛋白转移酶(FabD)基因的表达量与油棕果肉中游离硬脂酸、油酸和棕榈酸的含量呈正相关。且相关系数均大于0.6,6个关键酶基因的表达对游离硬脂酸、油酸和棕榈酸的累积具有积极的促进作用,而与游离月桂酸含量呈显著负相关,且相关系数均小于-0.8,6个关键酶基因的表达对游离月桂酸的合成起到抑制作用。本研究结果可为改善棕榈油品质提供科学的理论依据。 展开更多
关键词 油棕(elaeis guineensis) 代谢组学 转录组学 油脂合成 游离脂肪酸
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油棕中果皮MYB转录因子家族的鉴定与表达分析 被引量:1
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作者 徐鑫 郑育声 李东栋 《分子植物育种》 CAS 北大核心 2021年第19期6277-6289,共13页
油棕(Elaeis guineensis Jacq.)是世界上最重要的油料作物之一,其中果皮含有的棕榈油极其丰富。MYB蛋白是一类广泛存在于真核生物中的转录因子,在植物的生长发育中发挥重要调控作用。本研究基于油棕中果皮转录组的测序结果为基础,利用... 油棕(Elaeis guineensis Jacq.)是世界上最重要的油料作物之一,其中果皮含有的棕榈油极其丰富。MYB蛋白是一类广泛存在于真核生物中的转录因子,在植物的生长发育中发挥重要调控作用。本研究基于油棕中果皮转录组的测序结果为基础,利用生物信息学对油棕MYB基因家族进行全面鉴定,并对这些基因的结构、保守域和果实发育不同时期动态表达进行研究,解析油棕MYB转录因子在油棕发育过程中的生物学功能。结果发现,本研究中的38个油棕MYB转录因子,包含26个R2R3-MYB型和8个MYB-related型,以及2个3R-MYB型,不包含4R-MYB类型;MYB蛋白编码约200~700个氨基酸,等电点在4.5~9.5之间;亚细胞定位预测结果显示,所有MYB基因均定位于细胞核中;油棕MYB蛋白具有相似的保守基序组成,38个基因主要分布在13条染色体上,含有1到10个外显子数目;油棕MYB转录因子与多个物种的MYB转录因子具有序列同源性和进化亲缘性;表达模式分析发现,MYB家族基因存在差异表达,且具有几种不同的表达趋势。该研究结果为今后油棕MYB转录因子的脂类代谢途径研究以及功能和调控机制提供重要的研究基础。 展开更多
关键词 油棕(elaeis guineensis) MYB转录因子 生物信息学 表达模式
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油棕植物激素相关基因的生物信息学分析
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作者 刘新星 曾异征 +1 位作者 李东栋 郑育声 《分子植物育种》 CAS CSCD 北大核心 2020年第11期3493-3501,共9页
油棕(Elaeis guineensis Jacq.)享有"世界油王"的称誉,油棕中果皮的棕榈油(Palm oil)含量非常丰富,占干重的80%。众所周知,赤霉素(gibberellins,GAs)、生长素(Auxin)和乙烯(Ethane)是分布较广的植物激素,参与多种植物的油脂... 油棕(Elaeis guineensis Jacq.)享有"世界油王"的称誉,油棕中果皮的棕榈油(Palm oil)含量非常丰富,占干重的80%。众所周知,赤霉素(gibberellins,GAs)、生长素(Auxin)和乙烯(Ethane)是分布较广的植物激素,参与多种植物的油脂代谢和脂肪酸积累过程,且这3种激合成相关基因素在油棕中的研究鲜有报道。基于前期对非洲油棕果壳转录组基础上,本研究针对油棕赤霉素相关基因EgGA20ox 1-D和EgGA20ox 2,生长素相关基因EgYUC1和乙烯相关基因EgACS,进行生物信息学分析及生物学功能预测。结果发现,EgGA20ox1-D、EgGA20ox 2、Eg YUC1和EgACS蛋白均为疏水性蛋白,且具有多个功能位点(糖基化位点和磷酸化位点)。除了EgYUC1蛋白有2个跨膜结构区外,其余无跨膜结构区。此外,这四类蛋白二级结构的主要元件为无规则卷曲和α螺旋。该研究结果对深入解析油棕这3类植物激素相关基因在油脂代谢中的功能及其调控机理提供科学参考。 展开更多
关键词 油棕(elaeis guineensis) 赤霉素 生长素 乙烯 生物信息学
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Spatial-temporal distribution of fatal yellowing in different oil palm genetic materials in eastern amazon
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作者 Bruno Borella Anhe Artur Vinicius Ferreira dos Santos +2 位作者 Thiago Alan Ferreira da Silva Lana Leticia Barbosa de Carvalho Paulo Roberto Silva Farias 《Information Processing in Agriculture》 EI 2022年第3期365-377,共13页
Oil palm (Elaeis guineensis Jacq.) is one of the agricultural crops with the greatest potentialfor vegetable oil production in Brazil. However, a disease of unknown etiology popularlyknown as Fatal Yellowin (FY) has c... Oil palm (Elaeis guineensis Jacq.) is one of the agricultural crops with the greatest potentialfor vegetable oil production in Brazil. However, a disease of unknown etiology popularlyknown as Fatal Yellowin (FY) has caused damage to Brazilian farmers particularly in theeastern region of the Amazon. So, the objective of this study was to evaluate the spatialdependence of FY on three oil palm genotypes, grown for many years in an organic produc-tion system in the Amazon region. The study area had 4104 ha, divided into 139 plots. Ineach plot, the monthly incidence of disease was monitored forming a database. The num-ber of diseased plants per year, number of accumulated diseased plants, number of dis-eased plants per hectare, growth rate of diseased plants and incidence of accumulateddisease were evaluated. The results indicated spatial distribution of the variables adjustedto the gaussian, spherical and exponential models, with predominance of the first model.This increases the purpose that FY is caused by biotic factors. The highest range achievedin the study was 2929 m indicating the susceptibility of the tested genotypes. Some plotsclose to the river had the highest incidence of the disease on the study, probably associatedwith higher soil moisture. 展开更多
关键词 elaeis guineensis Geostatiscal analysis KRIGING Palm oil Spatial dependence
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