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Proliferation of Microorganisms in Acidic Fermentation of Elaeis guineensis L. Waste
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作者 M. Adedolapo Orimoloye A. Isaac Sanusi 《Advances in Microbiology》 2016年第9期644-649,共6页
An investigation into the type of fermentation oil palm fruit waste undergoes and the probable microorganisms involved within a short period was carried out using simple fermenter. The temperature was determined using... An investigation into the type of fermentation oil palm fruit waste undergoes and the probable microorganisms involved within a short period was carried out using simple fermenter. The temperature was determined using mercury thermometer, the pH of the medium was monitored with calibrated pH meter and the titratable acidic was determined using standard technique. The microbial profile of the medium was also evaluated using standard procedures. The highest temperature value was observed at day 0 (32.65&deg;C) and the lowest at day 1 (29.50&deg;C). The pH values of the fermentation oil palm fruit waste ranged between 4.15 - 4.60. The highest pH value was obtained at day 3 of the fermentation which was 4.60. The titratable acidity showed variation from day 0 - 2 and then with a continuous decrease till day 5. The least titratable acidity was obtained at day 5 (0.03) and the highest at day 2 (0.77). Bacteria load decreases from 1.0 × 10<sup>8</sup> - 1.6 × 10<sup>7 </sup>cfu/ml, while the fungi population increases from day 0 to day 5 of the fermentation period (1 × 10<sup>3</sup> - 2 × 10<sup>4 </sup>sfu/ml). Bacterial isolates obtained were Micrococcus leteus, Proteus vulgaris, Bacillus cereus, Baccillus subtilis, and Staphylococcus aureus while the fungal isolates obtained were Aspergillus niger, Neurospora crassa, Brachysporium spp, and Saccharomyces cerevisiae. It can be concluded that oil palm waste fermentation is an acidic fermentation that involved mesophiles microbes. And with these, several tons of oil palm epicarp waste can be optimally fermented (though with further research) and used for other purposes thereby reducing environmental pollution that would have resulted leaving this oil palm fruit waste in the environment. 展开更多
关键词 elaeis guineensis l. FERMENTATION MICROORGANISM PROlIFERATION WASTE
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油棕(Elaeis guineensis)中果皮发育过程中miRNA的表达动态分析 被引量:5
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作者 方良 梁远学 +2 位作者 李东栋 曹献英 郑育声 《植物科学学报》 CAS CSCD 北大核心 2013年第3期304-312,共9页
以CTAB法提取油棕(Elaeis guineensis)中果皮5个不同发育时期(G1~G5)的小RNA。从前期研究获得的油棕小RNA测序数据库中筛选12个候选miRNA,实时荧光定量PCR法(qRT-PCR)检测其在果实发育过程中的表达量变化,并进一步对显著差异表达的miRN... 以CTAB法提取油棕(Elaeis guineensis)中果皮5个不同发育时期(G1~G5)的小RNA。从前期研究获得的油棕小RNA测序数据库中筛选12个候选miRNA,实时荧光定量PCR法(qRT-PCR)检测其在果实发育过程中的表达量变化,并进一步对显著差异表达的miRNA进行靶基因预测。结果表明:中果皮5个不同发育时期小RNA的OD260/OD280比值在1.7~2.0之间;浓度分别是289、364、476、213、390 ng/μL;qRT-PCR检测结果显示,12个候选miRNA在5个发育时期均显著性差异表达,特别是在中果皮发育第4个时期(G4)和第5个时期(G5)表达量极显著增高,其中miR395和miR156在第4个时期表达量最高;miR395和miR528在发育第5时期表达量最高;靶基因预测结果显示差异表达的部分miRNA,其靶基因可能参与了脂肪酸代谢通路,如磷脂酸磷酸脂酶和磷脂酶D。本研究筛选的与脂肪酸代谢相关的miRNA为今后油棕脂肪酸代谢调控通路研究提供了可能的线索。 展开更多
关键词 油棕 脂肪酸代谢 MIRNA 实时定量PCR 靶基因预测
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Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines 被引量:1
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作者 Soundararajan Vijayarathna Sreenivasan Sasidharan 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2012年第10期826-829,共4页
Objective:To investigate the cytotoxic effect of Elaeis guineensis methanol extract on MCF-7and Vero cell.Methods:In vitro cytotoxicity was evaluated in by MTT assay.Cell morphological changes were observed by using l... Objective:To investigate the cytotoxic effect of Elaeis guineensis methanol extract on MCF-7and Vero cell.Methods:In vitro cytotoxicity was evaluated in by MTT assay.Cell morphological changes were observed by using light microscope.Results:The MTT assay indicated that methanol extract of the plant exhibited significant cytotoxic effects on MCF-7.Morphological alteration of the cell lines after exposure with lilaeis guineensis extract were observed under phase contrast microscope in the dose dependent manner.Conclusions:The results suggest the probable use of the Elaeis guineensis methanol extract in preparing recipes for cancer-related ailments.Further studies on isolation of metabolites and their in vivo cytotoxicity are under investigation. 展开更多
关键词 CYTOTOXICITY Growth INHIBITORY elaeis guineensis CEll MORPHOlOGY
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Morphogenesis of Oil Palm Fruit (Elaeis guineensis Jacq.) in Mesocarp and Endocarp Development 被引量:1
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作者 Hermine Bille Ngalle Joseph Martin Bell +3 位作者 Georges Franck Ngando Ebongue Lambert Nyobe Felix Chancelin Ngangnou Godswill Ntsefong Ntsomboh 《Journal of Life Sciences》 2013年第2期153-158,共6页
This work aims to study the development of the pericarp of the fruit of Elaeis guineensis Jacq. var. dura. The thickness, the water and the oil contents of its tissues are evaluated every two weeks, from pollination t... This work aims to study the development of the pericarp of the fruit of Elaeis guineensis Jacq. var. dura. The thickness, the water and the oil contents of its tissues are evaluated every two weeks, from pollination to the maturity of the fruit. The development of the oil palm fruit takes 5.5 months. The endocarp reaches its maximum thickness at the 70th DPP (day post-pollination), with a water content of 72%. It then starts its dehydration, while sclerifying. It therefore isolates the seed at start and later protects it. The mesocarp is visible at anthesis and its water content is close to 92%. From the 100th DPP, it begins a continuous dehydration associated, from the 130th DPP, with an active lipids biosynthesis. Ultimately, the pericarp of the oil palm fruit fulfills both functions, namely to protect the seed by early sclerification of the endocarp and ensure the dissemination of the species by the high oil content of the mesocarp. A comparative anatomy of the pericarp tissues of the three genotypes.of E. guineensis Jacq., during the first three weeks of fruit development, will enhance the understanding of the primary effect of sh gene. 展开更多
关键词 elaeis guineensis Jacq. FRUIT ENDOCARP MESOCARP development.
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Changes in calcium accumulation and utilization efficiency and their impact on recycling,immobilization,and export across the oil palm cycle
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作者 Ismael de Jesus Matos Viégas Luma Castro de Souza +4 位作者 Eric Victor de Oliveira Ferreira Milton Garcia Costa Glauco André dos Santos Nogueira Vitor Resende do Nascimento Candido Ferreira de Oliveira Neto 《Oil Crop Science》 CSCD 2024年第3期143-150,共8页
Effective calcium(Ca)management is crucial for optimizing oil palm cultivation and enhancing crop yield.This study aimed to gain insights into the dynamics of Ca concentration,accumulation,exportation,immobilization,a... Effective calcium(Ca)management is crucial for optimizing oil palm cultivation and enhancing crop yield.This study aimed to gain insights into the dynamics of Ca concentration,accumulation,exportation,immobilization,and recycling in various oil palm organs relative to plant age.The experiment was conducted at the Agropalma enterprise site in the northeastern region of Para State,Brazil,evaluating seven plant age treatments:2,3,4,5,6,7,and 8 years old.Employing a completely randomized design with four replications.The results demonstrated an age-related increase in Ca concentration in petioles,rachis,arrows,male inflorescences,peduncles,and fruits.Furthermore,Ca accumulation exhibited an upward trend in all organs with progressing plant age.Notably,the study revealed an enhanced Ca use efficiency across all plant organs in correlation with the age of oil palm cultivation.These findings underscore the dynamic nutritional demands of oil palm,influencing Ca immobilization,cycling,and export throughout its developmental stages. 展开更多
关键词 elaeis guineensis Jacq Ca cycling Ca export Ca immobilization Ca use efficiency Plant nutrition AMAZON
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Genetic Diversity and Interrelationship among Some Dura ×Tenera Oil Palm (Elaeis guineensis Jacq,) Genotypes in Cameroon
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作者 Mondjeli Constantin Sobir Ridwani +2 位作者 Muhamad Syukur Willy Bayuardi Suwamo Ntsomboh-Ntsefong Godswill 《Journal of Agricultural Science and Technology(A)》 2017年第2期81-90,共10页
In this study, the multivariate tools, namely principal component analysis (PCA) and cluster analysis, were used to classify and measure the pattern of genetic diversity and evaluate the correlation of nine oil palm... In this study, the multivariate tools, namely principal component analysis (PCA) and cluster analysis, were used to classify and measure the pattern of genetic diversity and evaluate the correlation of nine oil palm traits in 25 progenies. Fresh fruit bunch weight (FFB), kernel to fruit (K/F) and kemel to bunch (K/B) ratios showed significant variance, while bunch number (BN), kernel yield (KY) and oil yield (OY) showed little variance. Positive significant correlation between these traits and yield was appreciated through PCA, where 90.55% of the variation was explained by the first three principal components. Progeny grouping was performed and revealed three clusters of oil palm progenies. Cluster I contained progenies with high production of FFB, BN, OY and KY, while low height increment (HI) of palm trees was found in cluster II. However, most of progenies with high mean values of bunch spikelet weight (SpW), average fruit weight (AFW), K/F and K/B were grouped in cluster III. This grouping could help oil palm breeders to identify progenies with the traits of interest for breeding and commercial seed production. 展开更多
关键词 Oil palm elaeis guineensis Jacq. cluster analysis CORRElATION genetic diversity principal component analysis.
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Morphogenesis of Oil Palm (Elaeis guineensis Jacq.) Fruit in Seed Development
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作者 Hermine Bille Ngalle Joseph Martin Bell +3 位作者 Georges Franck Ngando-Ebongue Hernild Eman-Evina Godswill Ntsefong Ntsomboh Armand Nsimi-Mva 《Journal of Life Sciences》 2014年第12期946-954,共9页
The place of the oil palm, Elaeis guineensis Jacq., in the market for fats of vegetable commodities makes it a strategic plant which requires continuous improvement. In this context, it seems appropriate to better des... The place of the oil palm, Elaeis guineensis Jacq., in the market for fats of vegetable commodities makes it a strategic plant which requires continuous improvement. In this context, it seems appropriate to better describe the effects of the Sh gene in the developing fruit. This study aims to set a benchmark for the development of the seed in the natural palm (Elaeis guineensis var. dura) Thus the growth and development of the two major seed tissues were monitored every two weeks from pollination to maturity of the fruit. The results show that the endosperm is still liquid six weeks after pollination. It then begins an accelerated development which leads it, 11 weeks later, to completely fill the seed cavity, with an average mass of 0.81 g. This mass remains stable until the maturity of the fruit. The embryo is only visible when the endosperm is gelatinous, around 70 DPP (days post-pollination). It then has an average length of 1.00 mm. At 126 DPP, the embryo has finished growing and measures 2.82 mm on average. This length also remains stable until 168 DPP (3.04 mm). In perspective, a detailed follow-up of the development of the zygote from the pollination to 100 DPP is proposed. In parallel, the analysis of the chemical composition of the endosperm between 100 DPP and 168 DPP is necessary. These two complementary studies will allow to better specifying the benchmark of seed development in Elaeis guineensis var. dura. 展开更多
关键词 elaeis guineensis Jacq. EMBRYO ENDOSPERM SEED DEVElOPMENT
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油棕组培苗Mantled变异研究进展 被引量:2
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作者 张大鹏 王永 +2 位作者 石鹏 金龙飞 曹红星 《热带农业科学》 2017年第12期52-55,69,共5页
油棕作为高产木本油料作物,因其较长的育种周期,组织培养技术成为遗传改良的主要手段,但该技术存在Mantled变异问题。从Mantled变异的出现与形态特征、Mantled变异的分子标记研发、Mantled变异候选基因的推定、油棕的表观遗传学四个方... 油棕作为高产木本油料作物,因其较长的育种周期,组织培养技术成为遗传改良的主要手段,但该技术存在Mantled变异问题。从Mantled变异的出现与形态特征、Mantled变异的分子标记研发、Mantled变异候选基因的推定、油棕的表观遗传学四个方面综述了Mantled变异当前的研究现状,并对后续研究做出了展望。随着油棕转座子基因karma功能的确定,Mantled变异早期检测技术将成为后续研究重点,恢复基因功能和开发分子标记用于剔除变异苗将是Mantled变异的两个重要研究方向。而早期检测技术的突破,将有利于推动油棕产业的发展。 展开更多
关键词 油棕 组织培养 Mantled 体细胞变异
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Toxicity and antifeedant activity of essential oils from three aromatic plants grown in Colombia against Euprosterna elaeasa and Acharia fusca(Lepidoptera:Limacodidae) 被引量:1
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作者 Ricardo Hernández-Lambrao Karina Caballero-Gallardo Jesus Olivero-Verbel 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2014年第9期695-700,共6页
Objective:To determine the biological effects of essential oils(EOs) isolated from Cymbopogon iiardus,Cymbopogon Jlexuosus and Cvrnbopogon marlinii grown in Colombia against two Lepidoptera larvae,common pests in the ... Objective:To determine the biological effects of essential oils(EOs) isolated from Cymbopogon iiardus,Cymbopogon Jlexuosus and Cvrnbopogon marlinii grown in Colombia against two Lepidoptera larvae,common pests in the oil palm.Methods:Specimens were captured in the field and the antifeedant activity and dermal contact lethality of EOs were measured against Acharia fusca and Euprosterna elaeasa(Lepidoptera:I.imacodidae) at various concentrations 0.002-0.600 μL/cm^3 and 0.002-8 μL/g,respectively.Results:All EOs exhibited strong antifeedant and toxicity activity toward Acharia fusca and Euprosterna elaeasa larvae.Cymbopogon marlinii oil was llie most active againsl both pest insect species,although all tested EOs were better than the synthetic;repellent IR535 on both insects.Conclusions:Colombian EOs have potential for integrated pest management programs in the oil palm industry. 展开更多
关键词 elaeis guineensis PEST lEPIDOPTERA Defoliators
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Towards Sustainable Oil Palm Plantation Management: Effects of Plantation Age and Soil Parent Material 被引量:1
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作者 Georges Kogge Kome Fritz Oben Tabi 《Agricultural Sciences》 2020年第1期54-70,共17页
This study was conducted to generate information required to guide plantation management in relation to replanting on soils derived from different parent materials. Six oil palm estates in coastal lowlands of southwes... This study was conducted to generate information required to guide plantation management in relation to replanting on soils derived from different parent materials. Six oil palm estates in coastal lowlands of southwest Cameroon were considered. Oil palm yield data (in t&#8901;ha&#8722;1 of fresh fruit bunch, FFB) and corresponding age of palms (in years after planting, YAP) were obtained for the various estates. In all the estates, average yields were &#8901;FFB&#8901;ha&#8722;1 and highly variable. Plantation age, solely, explained between 20% - 58% of the variation in yield. The highest average yields (11.5 t&#8901;FFB&#8901;ha&#8722;1) were obtained in plantations aged between 9 and 18 YAP and the lowest (4.66 t&#8901;FFB&#8901;ha&#8722;1) were obtained in old plantations (>23 YAP). Plantations located on volcanic parent materials generally had higher yields compared to those established on sedimentary parent materials. In order to intensify production and increase yields while conserving the environment, one important measure to consider is the replacement of aged palms, and the recommended optimal replanting age in coastal plains of southwest Cameroon should be at most 23 YAP. Estimated mean yields, if aged palms are replanted on time, can increase by 43% - 65%. Additionally, site-specific nutrient management options should be considered in plantation intensification programs. 展开更多
关键词 elaeis guineensis Yield Variation COASTAl PlAIN Soils INTENSIFICATION Oil PAlM REPlANTING Cameroon
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Assessment of Genetic Diversity of NIFOR Oil Palm Main Breeding Parent Genotypes Using Microsatellite Markers
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作者 Maxwell N. Okoye Michael I. Uguru +2 位作者 Claude Bakoumé Rajinder Singh Christy O. Okwuagwu 《American Journal of Plant Sciences》 2016年第1期218-237,共20页
The genetic diversity among 15 NIFOR breeding parents was assessed using 10 microsatellite markers. A high genetic diversity was observed with a total of 64 alleles including 23 rare alleles or alleles at frequencies ... The genetic diversity among 15 NIFOR breeding parents was assessed using 10 microsatellite markers. A high genetic diversity was observed with a total of 64 alleles including 23 rare alleles or alleles at frequencies less than 0.05. The NIFOR tenera parents recorded the highest number of rare alleles. The average observed heterozygosity and mean gene diversity across all parental groups were 0.6889 and 0.7029, respectively. Higher genetic diversity was detected among the NIFOR dura and tenera parents compared to that of the Deli dura parents in absolute terms. Analysis of molecular variance (AMOVA) showed that 87% of the total variation (p < 0.001) observed was due to differences among parents. Rogers’ genetic distance ranged from 0.2988 to 0.8000 (mean = 0.5570). The dendrogram constructed on the basis of Rogers’ genetic distance clustered the parents in three groups. They generally clustered in heterotic manner rather than by geographic origins. The groupings obtained through PCoA confirmed the results obtained by cluster analysis. The results obtained are strong assets for NIFOR breeding programme. 展开更多
关键词 Allelic Diversity elaeis guineensis Jacq Genetic Distance HETEROSIS Molecular Characterization NIFOR Population Genetics Selection SSR Markers
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Utilization of Mill Effluent for Growth, Availability and Uptake of Nutrients by Palm Oil Seedlings
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作者 Nenny Nurlaeny Mahfud Arifin Denny Sobardini 《Journal of Agricultural Science and Technology(B)》 2017年第5期332-338,共7页
A large amount of palm oil mill effluent is one of the issues faced by palm oil producers in Indonesia. To alleviate the environmental pollution, it is necessary to reduce the problem by using it as a liquid organic f... A large amount of palm oil mill effluent is one of the issues faced by palm oil producers in Indonesia. To alleviate the environmental pollution, it is necessary to reduce the problem by using it as a liquid organic fertilizer. A pot experiment to determine the effects of mill effluent on growth of palm oil seedlings (Elaeis guineensis Jacq.), availability and uptake of macronutrients was conducted in Tandun Plantation Unit, province of Riau, Indonesia. The experiment used a randomized block design, consisting nine combinations of mill effluent doses (3.2, 6.4 and 9.6 L) and recommended fertilizer doses (100%, 75%, 50% NPKMg) in 20 kg soil and one control. The experiment was replicated three times. The results showed that plant height, stem diameter and number of fronds of palm oil seedlings at 26 weeks after planting (WAP) were significantly affected by application of 9.6 L mill effluent with 50%-75% NPKMg, The enhancement of soil organic C (Co,g) content, soil pH and cation exchange capacity due to the application of 9.6 L mill effluent combined with 50% NPKMg caused the availability of soil P and total N (Ntot) increased significantly, while exchangeable K was affected by application of 6.4 L mill effluent combined with 100% NPKMg. All treatments did not affect soil exchangeable Mg. A positive correlation between availability of soil N, P, K and its uptake by palm oil seedlings at 26 WAP were indicated by r = 0.61, 0.63 and 0.57, respectively. 展开更多
关键词 K Mg mill effluent N palm oil elaeis guineensis Jacq.) phosphate.
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Modelling Causes of Temporal Genotype-By-Environment Interaction in Oil Palm Bunch Yield in Nigeria
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作者 M. N. Okoye C. O. Okwuagwu +1 位作者 M. I. Uguru K. P. Baiyeri 《Journal of Agricultural Science and Technology(B)》 2011年第2期241-250,共10页
The objectives of this research were to understand pattern of temporal (year to year) stability in oil palm genotypes and then identify weather variable(s) associated with temporal stability. Stability analysis wa... The objectives of this research were to understand pattern of temporal (year to year) stability in oil palm genotypes and then identify weather variable(s) associated with temporal stability. Stability analysis was performed using the GGE biplot method. The best genotype in one year was not same always in the other year due to changes in the weather conditions over the years. Test environment evaluation revealed that the year 2000 (Y00) was the best representative of the overall environments and most powerful to discriminate genotypes with superior mean yield and temporal stability. The relationship of the climatic factors on the basis of their joint contribution to the development of specific trait varied from the pattern observed when their roles on the same traits were individually assessed. Maximum relative humidity, sunshine and rainfall could help explain the yield fluctuation due to their contribution to the overall yield variation. However, genotypes GI0 and G2 which exhibited high mean performance and stability for BN would be beneficial to the oil palm growers in ensuring steady cash flow from regular bunch yield production. 展开更多
关键词 elaeis guineensis Jacq GGE biplot analysis genotype x year interaction climatic factors principal component.
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Germination of Seeds of Interspecific Hybrid Caiaue×Oil Palm Submitted to the Mechanical Depulping
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作者 Thais Moura Maquine Alex Queiroz Cysne +3 位作者 Wanderlei Antonio Alves de Lima Samuel Campos Abreu Marcia Green Sara de Almeida Rios 《American Journal of Plant Sciences》 2014年第20期2965-2972,共8页
Oil palm seeds respond well to protocols of dormancy break, however, the seeds of interspecific hybrid (HIE) (caiaué × oil palm) have lower germination rates. The mechanical depulping can affect the potentia... Oil palm seeds respond well to protocols of dormancy break, however, the seeds of interspecific hybrid (HIE) (caiaué × oil palm) have lower germination rates. The mechanical depulping can affect the potential of germination of seeds, therefore the objective of this study was to evaluate the mechanical depulping on the seed germination, vigor and viability of embryos HIE BRS Manicoré. A randomized block design with five treatments, based on the depulping time and on the percentage of mesocarp on the seed with four replications was used. The percentage of seed germination, the germination speed index (GSI), the percentage of fungi and abnormal seeds and vigor and viability of embryos were evaluated. There was no statistical difference (p < 0.05) between treatments for all variables. The average of germination at 35 days was 45.34% and embryos showed high to medium vigor by the tetrazolium test. The mechanical depulping do not affect the seed germination, nor the viability and vigor of embryos HIE BRS Manicoré. It is recommended that the fruits of HIE BRS Manicoré remain in depulper machine for 40 minutes to completely remove the mesocarp residue. 展开更多
关键词 elaeis oleifera E.guineensis Percentage of Germination Mechanical Damage Tetrazolium Test
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油棕(Elaeis guineensis Jacq.)中果皮3个生长时期酚类化合物含量变化分析
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作者 李小丽 石佳 +2 位作者 孙汝浩 李东栋 郑育声 《分子植物育种》 CAS CSCD 北大核心 2017年第6期2394-2402,共9页
以油棕果为材料,分别提取3个生长时期(G1~G3)油棕中果皮酚类物质,采用高效液相色谱法分离,检测其中没食子酸、原儿茶酸、咖啡酸、丁香酸、香草酸、阿魏酸、香豆素等成分和含量,并分析其变化规律。结果表明,所采用提取和分离方法可获得... 以油棕果为材料,分别提取3个生长时期(G1~G3)油棕中果皮酚类物质,采用高效液相色谱法分离,检测其中没食子酸、原儿茶酸、咖啡酸、丁香酸、香草酸、阿魏酸、香豆素等成分和含量,并分析其变化规律。结果表明,所采用提取和分离方法可获得油棕中果皮中没食子酸、原儿茶酸、咖啡酸、丁香酸、香草酸、阿魏酸、香豆素,并完全分离。油棕中果皮3个发育时期总酚类化合物含量分别为:677.6μg/10 g、1 384.1μg/10 g和1 456.2μg/10 g,表明随着油棕果实发育成熟,酚类物质含量也随之增加。可溶性游离形态(SFP)、不溶性结合形态(ISBP)和酯化形态(EFP)3种酚类化合物中ISBP和EFP的含量较为丰富;EFP呈现极显著升高趋势。7种酚酸物质中,香草酸、阿魏酸和香豆素的含量相当丰富,香草酸含量尤为凸显,3个不同发育时期的含量分别为185.9μg/10 g、959.4μg/10 g、1 009.7μg/10 g,各占总酚类化合物的27.4%、69.3%、69.3%。咖啡酸、丁香酸和香草酸随油棕中果皮发育成熟含量递增;而没食子酸、原儿茶酸和阿魏酸则呈递减趋势,其中阿魏酸最为显著。 展开更多
关键词 油棕 酚类化合物 高效液相色谱
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山核桃(Carya cathayensis)与油棕(Elaeis guineensis)成油基因的比较 被引量:1
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作者 唐研耀 陈文充 +1 位作者 徐亚楠 曾燕如 《分子植物育种》 CAS CSCD 北大核心 2020年第23期7688-7699,共12页
山核桃是木本油料树种中含油率极高的经济树种。为了识别山核桃成油的关键基因,本研究将山核桃种子生长发育期胚转录组数据与油棕中果皮发育期转录组数据进行了比对分析。结果发现山核桃与油棕比对上的脂类代谢及碳水化合物代谢基因比... 山核桃是木本油料树种中含油率极高的经济树种。为了识别山核桃成油的关键基因,本研究将山核桃种子生长发育期胚转录组数据与油棕中果皮发育期转录组数据进行了比对分析。结果发现山核桃与油棕比对上的脂类代谢及碳水化合物代谢基因比例很高,其中有15个脂类代谢基因在油棕中有表达,而在山核桃中表达量极低;有35个脂类代谢基因在山核桃中相对表达量较高,在油棕中相对表达量较低,但只有14个基因与种子成油有关。有13个碳水化合物代谢相关基因在油棕中表达,而在山核桃中表达量低或并不是各时间点都能检测到;有26个基因在山核桃中表达,而在油棕中表达量低或未检测到,且所有这些基因均与脂肪酸合成的碳源供应有关。山核桃胚中识别的这些基因有3个基因的表达与含油率变化趋势一致,且在山核桃和油棕中的表达模式相似,但只有基因OBO(编码Oil-body oleosin,油体油菜素)表达量很高;油棕中高表达的基因山核桃中有8个,但它们的表达量均不如基因OBO。研究说明不同油料物种的成油相关基因表达有异同,基因OBO对山核桃高油脂的积累具有特殊的作用。 展开更多
关键词 山核桃(Carya cathayensis) 油棕 成油基因 脂类代谢 碳水化合物代谢
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低温胁迫对10个油棕新品种生理生化特性的影响 被引量:23
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作者 李静 陈秀龙 +2 位作者 李志阳 郑永清 曹建华 《华南农业大学学报》 CAS CSCD 北大核心 2013年第1期62-66,共5页
对10个油棕Elaeis guineensis Jacg新品种(RYL31~RYL40)半年生实生苗进行短期低温处理,测定油棕在低温逆境下的生理生化指标.结果表明:随着温度的降低,植物叶片叶绿素含量表现为先上升后下降然后再上升的趋势;游离脯氨酸、可溶性蛋白... 对10个油棕Elaeis guineensis Jacg新品种(RYL31~RYL40)半年生实生苗进行短期低温处理,测定油棕在低温逆境下的生理生化指标.结果表明:随着温度的降低,植物叶片叶绿素含量表现为先上升后下降然后再上升的趋势;游离脯氨酸、可溶性蛋白呈现先升高后降低的趋势;油棕可溶性糖含量受温度影响较大,且随低温胁迫强度的增加而增加;丙二醛(MDA)含量、质膜相对透性随温度降低表现为先下降后上升的趋势,其中RYL37、RYL38和RYL39品种的MDA含量一直呈下降趋势;不同生理指标对低温的敏感度不同,轻度低温能刺激植物防御机能增强,重度低温则会扰乱植物本身的防御机能. 展开更多
关键词 油棕 生理生化特性 低温胁迫
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基于SSR分子标记的油棕遗传多样性分析 被引量:11
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作者 周丽霞 吴翼 肖勇 《南方农业学报》 CAS CSCD 北大核心 2017年第2期216-221,共6页
【目的】应用SSR分子标记对8个油棕品种进行遗传结构及多样性分析,以期通过分析具有高杂合度油棕品种的遗传结构来辅助育种。【方法】利用SSR分子标记及PCR银染显色技术筛选多态性引物,分析8个油棕品种的等位基因频率(P)、观测杂合度(Ho... 【目的】应用SSR分子标记对8个油棕品种进行遗传结构及多样性分析,以期通过分析具有高杂合度油棕品种的遗传结构来辅助育种。【方法】利用SSR分子标记及PCR银染显色技术筛选多态性引物,分析8个油棕品种的等位基因频率(P)、观测杂合度(Ho)和期望杂合度(He),计算每个转座子的平均等位基因(Na)、每个多态转座子的平均等位基因数(Na/pl)及有效等位基因数(Ne),计算固定指数(Fis)和F-统计量值(Fit和Fst),并基于遗传距离对8个油棕品种进行聚类分析。【结果】开发出27对多态性SSR引物,从中选取15对结果较好的多态性引物对油棕大样本进行检测,挖掘出57个等位基因(Na),平均每个转座子的Na为3.8个,表明8个油棕品种的遗传变异较明显。平均有效等位基因数(Na)和期望杂合度(He)分别为0.6248和0.5902。此外,发现一个高水平的种群分化,F-统计量值(Fst)变化范围为0.1029~0.6010,平均为0.3664。利用多态性SSR分析8个油棕品种的遗传距离,结果发现品种1和品种3的遗传距离最远(1.674),品种3和品种5的遗传距离最近(0.065)。【结论】8个油棕品种的多态性相对丰富,物种间杂交程度较小,物种亲缘关系较远,遗传多样性良好。 展开更多
关键词 油棕 SSR分子标记 遗传多样性 遗传距离
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油棕果实不同发育时期类胡萝卜素的含量变化 被引量:5
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作者 李小丽 梁远学 +2 位作者 郜凌超 李东栋 郑育声 《华中农业大学学报》 CAS CSCD 北大核心 2015年第1期23-27,共5页
以油棕果为材料,分别提取油棕果5个发育时期的类胡萝卜素,采用高效液相色谱法进行分离,鉴定其中叶黄素、蕃茄红素、α-胡萝卜素、β-胡萝卜素的成分和含量,并分析其变化规律。结果表明,高效液相色谱法可对油棕果中的叶黄素、蕃茄红素、... 以油棕果为材料,分别提取油棕果5个发育时期的类胡萝卜素,采用高效液相色谱法进行分离,鉴定其中叶黄素、蕃茄红素、α-胡萝卜素、β-胡萝卜素的成分和含量,并分析其变化规律。结果表明,高效液相色谱法可对油棕果中的叶黄素、蕃茄红素、α-胡萝卜素、β-胡萝卜素完全分离并进行定性和定量分析。随着油棕果实成熟度的增加,α-胡萝卜素和β-胡萝卜素的含量逐渐增加,其中在果实发育后期,β-胡萝卜素是α-胡萝卜素的3~4倍;相反地,叶黄素从青果期到成熟期的含量是逐渐减少的;蕃茄红素的含量变化不大。油棕在成熟过程中总类胡萝卜素含量不断增加,主要转化为以α-胡萝卜素和β-胡萝卜素为主。 展开更多
关键词 油棕 类胡萝卜素 高效液相色谱 叶黄素 蕃茄红素 α-胡萝卜素 Β-胡萝卜素
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2012年广东江门油棕寒害调查及品种抗寒性分析 被引量:5
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作者 李静 陈秀龙 +4 位作者 马帅鹏 郑丽 冯朝阳 陶忠良 曾宪海 《农学学报》 2013年第11期1-4,共4页
为了比较油棕新品种RYL31—40 10个油棕新品种的抗寒性,在经历了2012年冬季低温寒害之后,于2013年4月对广东省江门市油棕抗寒种植前哨点的油棕试种基地进行调查,测定各油棕新品种的寒害指数。参考《油棕品种区域适应性试种观测技术规范... 为了比较油棕新品种RYL31—40 10个油棕新品种的抗寒性,在经历了2012年冬季低温寒害之后,于2013年4月对广东省江门市油棕抗寒种植前哨点的油棕试种基地进行调查,测定各油棕新品种的寒害指数。参考《油棕品种区域适应性试种观测技术规范》中寒害调查方法,将所调查的油棕进行分级,然后根据权数计算寒害指数。结果表明,RYL38和RYL32的寒害指数较大,分别为0.286、0.270,RYL40、RYL31和RYL39的寒害指数相对较小,分别为0.157,0.177和0.183。以寒害指数作为评价油棕新品种抗寒性的指标,结果得出10个不同新品种油棕在江门地区能够顺利过冬,其中RYL40、RYL31和RYL39的抗寒性相对较强。 展开更多
关键词 油棕 寒害 抗寒性 江门
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