The integration of implants or medical devices into the body tissues requires of good cell–material interactions.However,most polymeric materials used for these applications lack on biological cues,which enhanced mid...The integration of implants or medical devices into the body tissues requires of good cell–material interactions.However,most polymeric materials used for these applications lack on biological cues,which enhanced mid-and long-term implant failure due to weak integration with the surrounding tissue.Commonly used strategies for tissue–material integration focus on functionalization of the material surface by means of natural proteins or short peptides.However,the use of these biomolecules involves major drawbacks such as immunogenic problems and oversimplification of the constructs.Here,designed elastin-like recombinamers(ELRs)are used to enhance poly(methyl methacrylate)surface properties and compared against the use of short peptides.In this study,cell response has been analysed for different functionalization conditions in the presence and absence of a competing protein,which interferes on surface–cell interaction by unspecific adsorption on the interface.The study has shown that ELRs can induce higher rates of cell attachment and stronger cell anchorages than short peptides,being a better choice for surface functionalization.展开更多
Alzheimer’s disease(AD)is a neurodegenerative disorder,and the etiology of AD has not been completely elucidated.It remains unknown how the components from the brain’s extracellular matrix(ECM),particularly fibrous ...Alzheimer’s disease(AD)is a neurodegenerative disorder,and the etiology of AD has not been completely elucidated.It remains unknown how the components from the brain’s extracellular matrix(ECM),particularly fibrous entities,may influence the pathogenesis of AD.Herein,we report that treatment with elastin-like polypeptides(ELPs),a component of the brain ECM,significantly increases the extracellular levels of AD-related amyloid-beta(Aβ)peptides and decreases intracellular Aβlevels in human microglial cell model HMC3 cells(HMC3).展开更多
基金This work was supported by the Spain’s Ministerio de Economı´a y Competitividad[projects MAT2008-06887-C03-01,MAT2010-15310,MAT2013-41723-R,MAT2013-42473-R,VA313U14 and VA244U13].X.P.acknowledges grant BES-2009-027524 from the Spain’s Ministerio de Economı´a y Competitividad.
文摘The integration of implants or medical devices into the body tissues requires of good cell–material interactions.However,most polymeric materials used for these applications lack on biological cues,which enhanced mid-and long-term implant failure due to weak integration with the surrounding tissue.Commonly used strategies for tissue–material integration focus on functionalization of the material surface by means of natural proteins or short peptides.However,the use of these biomolecules involves major drawbacks such as immunogenic problems and oversimplification of the constructs.Here,designed elastin-like recombinamers(ELRs)are used to enhance poly(methyl methacrylate)surface properties and compared against the use of short peptides.In this study,cell response has been analysed for different functionalization conditions in the presence and absence of a competing protein,which interferes on surface–cell interaction by unspecific adsorption on the interface.The study has shown that ELRs can induce higher rates of cell attachment and stronger cell anchorages than short peptides,being a better choice for surface functionalization.
基金from National Key R&D Program of China(grant no.2018YFA0902600)the Scientific Instrument Developing Project of the Chinese Academy of Sciences(grant no.ZDKYYQ20180001)+1 种基金the Jilin Province Science Fund for Excellent Young Scholars(20190103072JH),K.C.Wong Education Foundation(grant no.GJTD-2018-09)and the National Natural Science Foundation of China(grant nos.21704099,21877104,and 21834007)and the Cure Alzheimer’s Fund.
文摘Alzheimer’s disease(AD)is a neurodegenerative disorder,and the etiology of AD has not been completely elucidated.It remains unknown how the components from the brain’s extracellular matrix(ECM),particularly fibrous entities,may influence the pathogenesis of AD.Herein,we report that treatment with elastin-like polypeptides(ELPs),a component of the brain ECM,significantly increases the extracellular levels of AD-related amyloid-beta(Aβ)peptides and decreases intracellular Aβlevels in human microglial cell model HMC3 cells(HMC3).
