Background:Collagen peptides(CP),including tripeptides and elastin peptides(EP),are known for their in vitro and in vivo anti-skin aging effects.Despite positive results in animal models,the combination effects of CP ...Background:Collagen peptides(CP),including tripeptides and elastin peptides(EP),are known for their in vitro and in vivo anti-skin aging effects.Despite positive results in animal models,the combination effects of CP and EP and the bioavailability of CP in human studies,particularly in young and middle-aged women,remain underexplored.Objective:To evaluate the effects of an orally administered collagen drink combining CP and EP on the skin health of young and middle-aged women.Materials and Methods:A single-center,randomized,double-blind,parallel-controlled trial was conducted,utilizing the WONDERLABR fish collagen tripeptide beverage.Participants consumed the drink over an 8-week period.Results:Compared to the placebo group,the collagen drink group showed significant improvements in skin hydration(39.19%increase),transepidermal water loss(33.45%decrease),skin elasticity(25.37%increase),dermal collagen content(21.64%increase),pore size(7.94%decrease),wrinkle length(18.09%decrease),skin smoothness(2.85%improvement),and skin roughness(15.32%decrease).Overall pore volume decreased by 60%,and visual assessments indicated a decrease in skin luminosity by 15.20%and smoothness index by 22.55%.Mass spectrometry demonstrated a significant increase in collagen efficacy components,including blood pH and GPH levels(P<0.05).Conclusion:The study confirmed the combination nourishing and anti-skin aging effects of EP and CP on the skin of young and middle-aged women,demonstrating significant improvements in various skin parameters and good bioavailability of collagen peptides.展开更多
Skin aging shows an imbalance between synthesis and degradation of the extracellular matrix. The overproduction of degradative enzymes (MMPs) during the chronology- and photo-induced aging leads to a degradation of th...Skin aging shows an imbalance between synthesis and degradation of the extracellular matrix. The overproduction of degradative enzymes (MMPs) during the chronology- and photo-induced aging leads to a degradation of the elastic and collagen networks. In a model of collagen and elastin destruction, we showed that the gingival fibroblast was able to preserve these macromolecules by inhibiting the overproduction of metalloproteinases by overproduction of TIMP-1 and modulation of the inflammatory cytokines activity. The objective of this study is to evaluate the effect of the gingival fibroblasts on human skin. The results in vitro and ex vivo show that the gingival fibroblast protects the skin collagen and elastic network by the inhibition of MMPs which leads to an overproduction of the TIMP-1. Moreover, the gingival fibroblast modulates the activity of some enzymes responsible for the inflammation;they inhibit the IL-1β and stimulate the production of TGF-β1. In vivo studies with a duration of six months and 50 women with pronounced wrinkles show that the culture supernatant of gingival fibroblasts diluted to 5% leads to a statistically significant decrease in the number and length of wrinkles.展开更多
为探究牦牛肺内纤维结构及其组成蛋白的增龄性变化,本研究采用Verhoeff’s Van Gieson(EVG)染色和网状纤维染色观察胶原纤维、弹性纤维及网状纤维在初生、幼年和成年牦牛肺组织中的分布情况;利用免疫组化、免疫荧光和蛋白免疫印迹对不...为探究牦牛肺内纤维结构及其组成蛋白的增龄性变化,本研究采用Verhoeff’s Van Gieson(EVG)染色和网状纤维染色观察胶原纤维、弹性纤维及网状纤维在初生、幼年和成年牦牛肺组织中的分布情况;利用免疫组化、免疫荧光和蛋白免疫印迹对不同年龄组牦牛肺组织中Ⅰ、Ⅲ、Ⅳ型胶原蛋白和弹性蛋白的分布特征及蛋白表达水平进行研究。结果显示,胶原纤维、网状纤维及三种胶原蛋白的分布位置基本一致,主要分布于牦牛肺脏支气管及血管外膜、软骨片、气管腺,支气管上皮细胞基膜、平滑肌层及肺泡隔也有较少量分布;而弹性蛋白及弹性纤维大量分布于支气管上皮细胞基膜、肺血管及肺泡隔中。四种蛋白的表达趋势基本一致,均在幼年组表达最高,初生组表达最低,且各年龄组间均差异显著(P<0.05)。结果表明,牦牛肺内弹性纤维、胶原纤维、网状纤维分布丰富,可使牦牛肺具有更好的收缩和舒张能力,有利于其对高寒缺氧环境的适应;Ⅰ、Ⅲ、Ⅳ型胶原蛋白和弹性蛋白表达量与年龄呈相关性,在幼年段表达最高,说明牦牛肺脏低氧适应性结构的形成在幼年段最为显著。本研究为进一步探究牦牛肺脏低氧适应结构提供了基础资料。展开更多
文摘Background:Collagen peptides(CP),including tripeptides and elastin peptides(EP),are known for their in vitro and in vivo anti-skin aging effects.Despite positive results in animal models,the combination effects of CP and EP and the bioavailability of CP in human studies,particularly in young and middle-aged women,remain underexplored.Objective:To evaluate the effects of an orally administered collagen drink combining CP and EP on the skin health of young and middle-aged women.Materials and Methods:A single-center,randomized,double-blind,parallel-controlled trial was conducted,utilizing the WONDERLABR fish collagen tripeptide beverage.Participants consumed the drink over an 8-week period.Results:Compared to the placebo group,the collagen drink group showed significant improvements in skin hydration(39.19%increase),transepidermal water loss(33.45%decrease),skin elasticity(25.37%increase),dermal collagen content(21.64%increase),pore size(7.94%decrease),wrinkle length(18.09%decrease),skin smoothness(2.85%improvement),and skin roughness(15.32%decrease).Overall pore volume decreased by 60%,and visual assessments indicated a decrease in skin luminosity by 15.20%and smoothness index by 22.55%.Mass spectrometry demonstrated a significant increase in collagen efficacy components,including blood pH and GPH levels(P<0.05).Conclusion:The study confirmed the combination nourishing and anti-skin aging effects of EP and CP on the skin of young and middle-aged women,demonstrating significant improvements in various skin parameters and good bioavailability of collagen peptides.
文摘Skin aging shows an imbalance between synthesis and degradation of the extracellular matrix. The overproduction of degradative enzymes (MMPs) during the chronology- and photo-induced aging leads to a degradation of the elastic and collagen networks. In a model of collagen and elastin destruction, we showed that the gingival fibroblast was able to preserve these macromolecules by inhibiting the overproduction of metalloproteinases by overproduction of TIMP-1 and modulation of the inflammatory cytokines activity. The objective of this study is to evaluate the effect of the gingival fibroblasts on human skin. The results in vitro and ex vivo show that the gingival fibroblast protects the skin collagen and elastic network by the inhibition of MMPs which leads to an overproduction of the TIMP-1. Moreover, the gingival fibroblast modulates the activity of some enzymes responsible for the inflammation;they inhibit the IL-1β and stimulate the production of TGF-β1. In vivo studies with a duration of six months and 50 women with pronounced wrinkles show that the culture supernatant of gingival fibroblasts diluted to 5% leads to a statistically significant decrease in the number and length of wrinkles.
文摘为探究牦牛肺内纤维结构及其组成蛋白的增龄性变化,本研究采用Verhoeff’s Van Gieson(EVG)染色和网状纤维染色观察胶原纤维、弹性纤维及网状纤维在初生、幼年和成年牦牛肺组织中的分布情况;利用免疫组化、免疫荧光和蛋白免疫印迹对不同年龄组牦牛肺组织中Ⅰ、Ⅲ、Ⅳ型胶原蛋白和弹性蛋白的分布特征及蛋白表达水平进行研究。结果显示,胶原纤维、网状纤维及三种胶原蛋白的分布位置基本一致,主要分布于牦牛肺脏支气管及血管外膜、软骨片、气管腺,支气管上皮细胞基膜、平滑肌层及肺泡隔也有较少量分布;而弹性蛋白及弹性纤维大量分布于支气管上皮细胞基膜、肺血管及肺泡隔中。四种蛋白的表达趋势基本一致,均在幼年组表达最高,初生组表达最低,且各年龄组间均差异显著(P<0.05)。结果表明,牦牛肺内弹性纤维、胶原纤维、网状纤维分布丰富,可使牦牛肺具有更好的收缩和舒张能力,有利于其对高寒缺氧环境的适应;Ⅰ、Ⅲ、Ⅳ型胶原蛋白和弹性蛋白表达量与年龄呈相关性,在幼年段表达最高,说明牦牛肺脏低氧适应性结构的形成在幼年段最为显著。本研究为进一步探究牦牛肺脏低氧适应结构提供了基础资料。
