Objective： To observe the influence of electroacupuncture （EA） at different time in a clay on the number of duodenal endocrine cells （argentaffin cells） in rats. Methods. Forty-eight male Wistar rats were evenly ...Objective： To observe the influence of electroacupuncture （EA） at different time in a clay on the number of duodenal endocrine cells （argentaffin cells） in rats. Methods. Forty-eight male Wistar rats were evenly randomized into control and electroaoupuncfure （EA） groups which were further divided into 5：00, 11：00, 17：00 and 23：00 subgroups respectively. EA （4- 16 Hz in the frequency, 3 V in the strength, intermittent dense-sparse waves, and duration of 20 min） was applied to bilateral “Huan- tiao” （环跳 GB 30） at 5：00 o＇clock, 11：00 o＇clock, 17：00 o＇clock and 23：00 o＇clock separately for different subgroups. After EA, the animals were killed at once for sempling the middle part of cluodenum which was fixed in 10% formalin for 2 hours, then embed in paraffin. The tissue was cut into sections （ 10 μm）, mounted on glass slides and then processed according to Grimelius＇s methods. Results： The rumber of argentaffin cells in EA group was significantly fewer than that in control group at the same timewindow （23：00, P〈0.05）. No significant differences were found between control and EA groups in the number of cells at other time window （5：00, 11：00 and 17：00 o＇clock, P〉0.05）, suggesting that EA of “Huantiao”can markedly promote the secretion activity of endocrine cells at 11：00 o＇clock during night ferent time window in a day. Conclusion. The results suggest that the effects of EA are different at clifferent time window in a day.展开更多
文摘Objective： To observe the influence of electroacupuncture （EA） at different time in a clay on the number of duodenal endocrine cells （argentaffin cells） in rats. Methods. Forty-eight male Wistar rats were evenly randomized into control and electroaoupuncfure （EA） groups which were further divided into 5：00, 11：00, 17：00 and 23：00 subgroups respectively. EA （4- 16 Hz in the frequency, 3 V in the strength, intermittent dense-sparse waves, and duration of 20 min） was applied to bilateral “Huan- tiao” （环跳 GB 30） at 5：00 o＇clock, 11：00 o＇clock, 17：00 o＇clock and 23：00 o＇clock separately for different subgroups. After EA, the animals were killed at once for sempling the middle part of cluodenum which was fixed in 10% formalin for 2 hours, then embed in paraffin. The tissue was cut into sections （ 10 μm）, mounted on glass slides and then processed according to Grimelius＇s methods. Results： The rumber of argentaffin cells in EA group was significantly fewer than that in control group at the same timewindow （23：00, P〈0.05）. No significant differences were found between control and EA groups in the number of cells at other time window （5：00, 11：00 and 17：00 o＇clock, P〉0.05）, suggesting that EA of “Huantiao”can markedly promote the secretion activity of endocrine cells at 11：00 o＇clock during night ferent time window in a day. Conclusion. The results suggest that the effects of EA are different at clifferent time window in a day.
