Seed Priming with MgCl_(2),CaCl_(2),and ZnCl_(2) as a Biofortification Based-Approach Induces Changes in Anise Seedlings Emergence

Aromatic and medicinal plant species having small seeds havefield emergence problems due to low nutrient supply.Therefore,Pimpinella anisum seeds were hydro and osmoprimed with 100 mM MgCl_(2),CaCl_(2),and ZnCl_(2),for 2,4,and 8 h each to compare their growth attributes during germination and seedling establishment stages.Nontreated seeds were used as control.Both hydro and osmo primed seeds were dried for 48 h before,they were sown in plastic trays in growth room conditions to see the impact of treatments on seedling emergence and growth.The maximum root length(12.90 cm),fresh weight(256.30 mg plant^(-1)),and mean emergence time(MET)were recorded from 8 h MgCl_(2) primed seeds.Similarly,the maximum(97.50,and 98.00%)emergence percentage was observed using 8 h MgCl_(2) primed seeds and nontreated seeds(control treatment).The evaluation of parameters like chlorophyll contents and electrical conductivity showed the 8 h MgCl_(2) priming as the optimum treatment.The evaluation of parameters like chlorophyll contents and electrical conductivity showed the 8 h MgCl_(2) priming as the optimum treatment.The result suggests MgCl_(2) priming worked synergistically and improved seed-ling growth attributes under greenhouse conditions.The chlorophyll content ranged 25.94–35.69 SPAD unit.The highest chlorophyll content was obtained from the seedlings obtained from 4 h CaCl_(2) treatment,which were statistically similar to the chlorophyll contents of the seedlings obtained after 8 h MgCl_(2) treatment and nontreated seeds(control treatment).All other treatments showed inhibition in the chlorophyll contents and growth attributes of the seedlings.In conclusion,MgCl_(2) osmopriming treatments were significantly promotive and better compared to hydro-priming and osmopriming treatments including control treatment in terms of anise seeds germination and emergence.

Assessing frost hardiness of Pinus bungeana shoots and needles by electrical impedance spectroscopy with and without freezing tests

Aims Nursery and forest operations require that frost hardiness results be produced faster than can be provided by controlled freezing tests.There is a great challenge to develop a rapid method for predicting frost hardiness that might not necessitate controlled freezing tests.The aim of this study was to examine the assessment of the frost hardiness of shoots and needles of Pinus bungeana by electrical impedance spectroscopy(EIS)with and without controlled exposure to freezing.Methods The frost hardiness of current-year shoots and needles of P.bungeana in an 8-year-old provenance field trial was measured at Shisanlin Nursery in Beijing,China,from September 2006 to January 2007 by means of EIS and conventional electrolyte leakage(EL).In the same plants,but without controlled freezing test,were monitored the EIS parameters in current-year shoots and needles.Important Findings The results showed that(i)after controlled freezing tests,the frost hardiness estimated by EIS parameters(extracellular resistance,re,and membrane time constant,sm)was significantly correlated with the frost hardiness assessed by EL method(r=0.95)and(ii)for the samples not exposed to controlled freezing treatment,the relaxation time s1 for shoots and b for needles had greater correlations with the frost hardiness estimated by EL after controlled freezing tests relative to the other parameters(r=
0.90 for shoots and r=0.84 for needles,respectively).The parameters re of shoots and needles and sm of needles might be applied for measuring frost hardiness of samples after exposed to controlled freezing tests.The frost hardiness results can be obtained within 48 h.The parameters s1 of shoots and b of needles could be used for estimating the frost hardiness of samples without using a controlled freezing test.The frost hardiness results can be obtained within 24 h.

Expression of multi-domain type III antifreeze proteins from the Antarctic eelpout (Lycodichths dearborni) in transgenic tobacco plants improves cold resistance

Type III antifreeze proteins(AFPIIIs)are a group of small globular proteins found in some polar fishes to protect them against freezing damage.Transgenic expression of AFPs has been shown to confer cold tolerance to commercially important plants and animals.We have previously isolated multiple AFPIII genes in the Antarctic eelpout(Lycodichthys dearborni)that encode larger AFPIII isoforms with up to 12 of the conventional domains.Here we have introduced the fish AFPIII genes that encode for the monomer(ld1),dimer(ld2),trimer(ld3)and tetramer(ld4)AFPIII isoforms in tobacco plants.Pot-grown 4-week-old transgenic tobacco plants were exposed to cold stress at 4◦C for 30 days and the results show that ld1,ld2,ld3 and ld4 transgenic plants present relatively lower electrolyte leakage and lower content of malondialdehyde(MDA),but accumulated higher content of proline when compared to control plants.This indicates considerable improved membrane integrity under low temperature stress and improvement of the plant cold resistance.The plants transformed with the AFPIII tetramer-and trimer-domains demonstrated a higher cold-tolerant levels when compared with plants transformed with the dimer-and monomer AFPIII domains.Our study further supports that fish AFPIIIs,especially the multidomain proteins,protect cells from non-freezing hypothermic stresses,apart from there well-known function as ice inhibitors molecules at freezing temperature.

基于受体修饰的双层有机场效应晶体管化学传感器用于高灵敏检测锂离子电池电解液泄漏

易燃易爆的锂离子电池电解液泄漏是电池故障的早期症状之一,甚至会导致电池自燃或电动汽车爆炸.因此,有必要找到一种快速简单的方法来监测电解液泄漏.现有的传感器难以有效且快速地检测微量电解液泄漏.我们首次提出了一种将有机场效应晶体管(OFET)的灵敏度和联二脲受体的选择性相结合,以检测电池电解液泄漏的策略.复合后的传感器与没有受体的原始传感器相比,灵敏度显著提高,传感器对碳酸二乙酯的检测限低至1.4 ppm.并且可以在几秒钟内有效地检测到微量的泄漏, 200 n L电解液泄漏能够导致3%的响应.受体复合后的OFET传感器的优异性能使其成为锂离子电池安全监测的良好候选者,并为传感技术的发展提供了一个有前途的平台.