In this research, 3-day-old etiolated wheat seedlings of Triticum aestivum L. cv. Ceyhan-99 （salt-sensitive） and T. durum Desf. cv. Firat-93 （salt-tolerant） were grown in control and salt （150 mmol/L NaCl） treat...In this research, 3-day-old etiolated wheat seedlings of Triticum aestivum L. cv. Ceyhan-99 （salt-sensitive） and T. durum Desf. cv. Firat-93 （salt-tolerant） were grown in control and salt （150 mmol/L NaCl） treatments at a 15/25℃ temperature regime in the light for 12 days. Soluble proteins extracted from the first leaf tissues of two cultivars were analyzed by twodimensional （2-D） electrophoresis in order to detect NaCl-induced changes. The soluble leaf protein profiles of cultivars were observed to be similar. However, quantitative differences in 74 proteins were detected in the salt treatment group, compared to the control. Among the 74 protein spots, 14 were common for two cultivars. As a result of NaCl treatment, two low-molecular-weight （LMW） proteins （28.9 and 30.0 kDa） and one intermediate-molecular-weight （IMW） protein （44.3 kDa） in cv. Ceyhan-99 and six LMW proteins （18.6, 19.4, 25.7, 25.9, 26 and 27.6 kDa） in cv. Firat-93 were newly synthesized. The newly synthesized proteins were specific to each cultivar. In the Firat-93 cultivar, four proteins with LMW （24.8-27.9 kDa） were completely lost in NaCl treatment. Moreover, these four protein spots were not observed in both protein profiles of cv. Ceyhan-99. Most of these proteins were in acidic character （pl 〈6.0-6.9） and low molecular weight （〈31.6 kDa）. It is suggested that the newly synthesized or completely lost LMW proteins may be important for cultivars differing in sensitivity towards NaCl.展开更多
The protein binding constant, binding sites of the Strychnos alkaloid-strychnine and bovine serum albumin （BSA） was determined by capillary electrophoretic frontal analysis （CE-FA） for the first time. The experime...The protein binding constant, binding sites of the Strychnos alkaloid-strychnine and bovine serum albumin （BSA） was determined by capillary electrophoretic frontal analysis （CE-FA） for the first time. The experiment was carried out in a polyacrylamide-coated fused silica capillary （48.4 cm×50 μm i.d., 38.1 cm effective length） with 20 mmol/L citrate/MES buffer （pH 6.0, ionic strength 0.17）. The applied voltage was 12 kV and detection wavelength was set at 257 nm. The plateau height of the peak was employed to determine the unbound concentration of drug in BSA equilibrated sample solution based on the external drug standard in the absence of protein. The present method provides a convenient, accurate technique for the early stage of drug screening.展开更多
The dromedary camel （Camelus dromedarius） is a significant socioeconomic importance in several arid and semi-arid regions of North Africa and Middle East, and its milk constitutes an important component of human die...The dromedary camel （Camelus dromedarius） is a significant socioeconomic importance in several arid and semi-arid regions of North Africa and Middle East, and its milk constitutes an important component of human diets in these regions. The camel milk plays a vital role in the food of the Algerian nomads in the Sahara. During February and September, 20 samples of the raw camel＇s milk were taken starting from different livestock of camels from three different Sahariennes regions （Bechar, EI-Bayadh and Naama）. These 20 collected samples were analyzed by physico-chemical and microbiological methods. The results of physicochemical analyze obtained from two hot and cold seasons are respectively the following： T ~C （35.83 and 33.95）, pH （6.36 and 6.49）, density （1.031 and 1.032）, dornic acidity （18.6 and 18.3 ~D）, dry matter （93.4 and 144.8 g/L）, fat contents （30 and 52.1 g/L）, total protein （26.3 and 33.1 g/L） and ashes （7.46 and 8.66 g/L）. The protein profile obtained by electrophoretic analysis （SDS-PAGE） showed that camel milk contains several types of proteins and some have a molecular weight identical to major proteins of the cow＇s milk. The final results showed that camel milk has generally a comparable composition to that of bovine milk. The microbiological analysis, of these samples, detected a significant number of the total microflora, Staphylococcus aureus and total coliforms. The absence of Clostridium and fecal coliforms was observed. Several species of lactic acid bacteria were detected such as Lactococcus lactis subsp, lactis, Lactococcus lactis subsp, lactis biovar, diacetylactis, Weissella cibaria and Enteroccocusfeacalis.展开更多
文摘In this research, 3-day-old etiolated wheat seedlings of Triticum aestivum L. cv. Ceyhan-99 （salt-sensitive） and T. durum Desf. cv. Firat-93 （salt-tolerant） were grown in control and salt （150 mmol/L NaCl） treatments at a 15/25℃ temperature regime in the light for 12 days. Soluble proteins extracted from the first leaf tissues of two cultivars were analyzed by twodimensional （2-D） electrophoresis in order to detect NaCl-induced changes. The soluble leaf protein profiles of cultivars were observed to be similar. However, quantitative differences in 74 proteins were detected in the salt treatment group, compared to the control. Among the 74 protein spots, 14 were common for two cultivars. As a result of NaCl treatment, two low-molecular-weight （LMW） proteins （28.9 and 30.0 kDa） and one intermediate-molecular-weight （IMW） protein （44.3 kDa） in cv. Ceyhan-99 and six LMW proteins （18.6, 19.4, 25.7, 25.9, 26 and 27.6 kDa） in cv. Firat-93 were newly synthesized. The newly synthesized proteins were specific to each cultivar. In the Firat-93 cultivar, four proteins with LMW （24.8-27.9 kDa） were completely lost in NaCl treatment. Moreover, these four protein spots were not observed in both protein profiles of cv. Ceyhan-99. Most of these proteins were in acidic character （pl 〈6.0-6.9） and low molecular weight （〈31.6 kDa）. It is suggested that the newly synthesized or completely lost LMW proteins may be important for cultivars differing in sensitivity towards NaCl.
基金The authors are grateful to the National Natural Science Foundation of China(Grant No.20235020 and 20372062)for financial support.
文摘The protein binding constant, binding sites of the Strychnos alkaloid-strychnine and bovine serum albumin （BSA） was determined by capillary electrophoretic frontal analysis （CE-FA） for the first time. The experiment was carried out in a polyacrylamide-coated fused silica capillary （48.4 cm×50 μm i.d., 38.1 cm effective length） with 20 mmol/L citrate/MES buffer （pH 6.0, ionic strength 0.17）. The applied voltage was 12 kV and detection wavelength was set at 257 nm. The plateau height of the peak was employed to determine the unbound concentration of drug in BSA equilibrated sample solution based on the external drug standard in the absence of protein. The present method provides a convenient, accurate technique for the early stage of drug screening.
文摘The dromedary camel （Camelus dromedarius） is a significant socioeconomic importance in several arid and semi-arid regions of North Africa and Middle East, and its milk constitutes an important component of human diets in these regions. The camel milk plays a vital role in the food of the Algerian nomads in the Sahara. During February and September, 20 samples of the raw camel＇s milk were taken starting from different livestock of camels from three different Sahariennes regions （Bechar, EI-Bayadh and Naama）. These 20 collected samples were analyzed by physico-chemical and microbiological methods. The results of physicochemical analyze obtained from two hot and cold seasons are respectively the following： T ~C （35.83 and 33.95）, pH （6.36 and 6.49）, density （1.031 and 1.032）, dornic acidity （18.6 and 18.3 ~D）, dry matter （93.4 and 144.8 g/L）, fat contents （30 and 52.1 g/L）, total protein （26.3 and 33.1 g/L） and ashes （7.46 and 8.66 g/L）. The protein profile obtained by electrophoretic analysis （SDS-PAGE） showed that camel milk contains several types of proteins and some have a molecular weight identical to major proteins of the cow＇s milk. The final results showed that camel milk has generally a comparable composition to that of bovine milk. The microbiological analysis, of these samples, detected a significant number of the total microflora, Staphylococcus aureus and total coliforms. The absence of Clostridium and fecal coliforms was observed. Several species of lactic acid bacteria were detected such as Lactococcus lactis subsp, lactis, Lactococcus lactis subsp, lactis biovar, diacetylactis, Weissella cibaria and Enteroccocusfeacalis.
